Validation of Liquid Chromatographic Method for Assay of Calcitriol and Alfacalcidol in Capsule Formulations
Keyword(s):
Abstract The validation of a liquid chromatographic procedure suitable for the determination of calcitriol and alfacalcidol in their respective formulations labeled to contain at least 0.25 μ.g drug per unit is described. The capsule content is diluted and chromatographed in 15-20 min on silica columns (5 μm) with a mobile phase of hexane-tetrahydrofuranmethylene dichloride-isopropanol (72 + 12 + 12 + 4, v/v) with detection at 254 nm. The calibration curve is linear. Recoveries of “spikes” averaged 101% with a standard deviation of 2%. Precision was better than 1.5%.
2009 ◽
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pp. 846-854
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1993 ◽
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