Use of Deuterated Internal Standards for Quantitation of T-2 and HT-2 Toxins in Human Blood by Tandem Mass Spectrometry
Abstract Deuterated acetyl derivatives (3-trideutero-acetyl-T-2 and 15-trideutero- HT-2) were prepared for use as internal standards for the quantitation of T-2 and HT-2 in blood by tandem mass spectrometry. The method used was multiple reaction monitoring (MRM), which essentially involves the selection of a parent ion for analysis followed by monitoring of the daughter ions generated by collision activated decomposition. The parent ions chosen for the trifluoroacetate derivative of T-2 and HT-2 were m/z+ 478 and 532, respectively. Both parents yield the same daughter ions, i.e., 180, 138, and 121. HT-2 and T-2 were added to blood extracts in amounts ranging from 1 to 20 ppb. The limit of detection is about 0.5 ppb with an effective detection limit of 1.0 ppb in a range of 1-20 ppb. The recovery is about 90%. This method can be used by veterinarians for purposes of diagnostics. It can be used for urine as well as blood.
Putative multiple reaction monitoring strategy for the comparative pharmacokinetics of postoral administration Renshen–Yuanzhi compatibility through liquid chromatography–tandem mass spectrometry
