Identification and Quantitation of Corticosteroids and Their Esters in Pharmaceutical Preparations of Creams and Ointments by Thin-Layer Chromatography and Densitometry

1994 ◽  
Vol 77 (6) ◽  
pp. 1435-1438 ◽  
Author(s):  
Kajal Datta ◽  
Saroj K Das
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Excellent Agreement ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Preparations ◽  
Insoluble Material ◽  
Ointment Base ◽  
Layer Chromatography

Abstract A rapid, simple, and specific thin-layer chromatographic (TLC) method is described for estimation of various corticosteroids and their esters when present in combination with other drugs in pharmaceutical preparation of creams and ointments. The cream or ointment is dissolved in chloroform; water and insoluble material, if any, are removed by centrifugation, and the steroid is separated from other ingredients by TLC on silica gel G with n-hexane as the first developing solvent (to wash out cream or ointment base) and chloroform–ethyl acetate (1 + 1 , v/v, for free steroids or 2 + 1 , v/v, for steroid esters) as the second developing solvent. The separated steroid or ester is visualized under shortwave UV (254 nm) and estimated by spectrodensitometry at 240 nm. Recoveries of the steroids are between 98.0 and 101.7%. Results were compared with those obtained by official methods, where applicable, and excellent agreement was found.

Identification and Quantitation of Panthenol and Pantothenic Acid in Pharmaceutical Preparations by Thin-Layer Chromatography and Densitometry

1992 ◽  
Vol 75 (5) ◽  
pp. 898-901 ◽  
Author(s):  
Saurendra S Nag ◽  
Saroj K Das
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Benzyl Alcohol ◽  
Silica Gel ◽  
Pantothenic Acid ◽  
Pharmaceutical Preparations ◽  
Ninhydrin Reaction ◽  
Layer Chromatography

Abstract A rapid, simple, and specific thin-layer chromatographic (TLC) method has been developed for the estimation of panthenol and pantothenic acid in pharmaceutical preparations containing other vitamins, amino acids, syrups, enzymes, etc. The vitamin is extracted with ethanol (tablets and capsules) or benzyl alcohol (liquid oral preparations) and isolated from other ingredients by TLC on silica gel with isopropanol-water (85 + 15, v/v) as developing solvent. β-Alanine (pantothenate) or β-alanol (panthenol) is liberated by heating for 20 min at 160°C. The liberated amines are visualized by ninhydrin reaction and estimated by spectrodensitometry at 490 nm. Recoveries for panthenol and pantothenic acid were 99.8 ± 2.25% and 100.2 ± 1.7%, respectively.

Identifikasi Sediaan Jamu Kuat yang Beredar di Kecamatan Banguntapan dan Pleret Kabupaten Bantul dengan Metode KLT

2019 ◽  
Vol 14 (2) ◽  
pp. 103
Author(s):  
Dewi Umniyatul ◽  
Dwi Kurniawati Sambodo
Keyword(s):  
Herbal Medicine ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Plant Material ◽  
Uv Light ◽  
Sildenafil Citrate ◽  
Quota Sampling ◽  
Layer Chromatography

Background: Traditional herbs are ingredients or herbs in the form of plant material, animal ingredients, mineral ingredients, galenic preparations or mixtures of these ingredients, it has been used as medicine traditionally.Objective: This study is aimed to Identify of Sildenafil Citrate on “Jamu Kuat” (Herbal Medicine) products.Methods: This type of research was descriptive and the sample was determined by quota sampling. The method used Thin Layer Chromatography (TLC) which was a physicochemical method. There were seven samples were extracted by the soxhletation method until a thick extract was obtained to be spotted in the quite phase of TLC silica gel F254. The motion phase used to identify sildenafil citrate was chloroform: ethyl acetate (6: 4). Spots detection was done by observation under UV light 366 nm and the spots that appeared were calculated of Rf value and compared with the comparable Rf value of sildenafil citrate.Results: From this study, there were samples containing sildenafil citrate. Sample C with a sample Rf value of 0.75 and sample D with Rf value of sample 0.78 and a comparable Rf of sildenafil citrate 0.77.Conclusion: The seven samples of “Jamu Kuat” contained sildenafil citrate in Banguntapan and Pleret Districts, Bantul Regency. Keywords: Jamu Kuat (Herbal Medicine), Sildenafil citrate, TLC

Improved Method for the Thin Layer Chromatographic Determination of Patulin in Apple Juice

1973 ◽  
Vol 56 (4) ◽  
pp. 813-816 ◽  
Author(s):  
Peter M Scott ◽  
Barry P C Kennedy
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Apple Juice ◽  
Chromatographic Determination ◽  
Improved Method ◽  
Layer Chromatography ◽  
Hydrochloride Solution

Abstract Apple juice from a freshly opened container is extracted 3 times with ethyl acetate. The extract is dried, concentrated, diluted with benzene, and added to a silica gel column. Patulin is eluted by benzene-ethyl acetate (75+25) and detected by thin layer chromatography, using a 3-methyl-2-benzothiazolinone hydrazone hydrochloride solution as the spray reagent. Satisfactory recoveries were obtained for patulin added to apple juice at levels of 25–400 μg/L.

scholarly journals Triterpenoids from the wood of Willughbeia cochinchinensis

2018 ◽  
Vol 1 (T5) ◽  
pp. 137-142
Author(s):  
Linh Thi My Lam ◽  
Phu Hoang Dang ◽  
Hai Xuan Nguyen ◽  
Nhan Trung Nguyen ◽  
Mai Thi Thanh Nguyen
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Column Chromatography ◽  
Normal Phase ◽  
Reverse Phase ◽  
Chemical Structures ◽  
First Time ◽  
Layer Chromatography

From the wood of n-hexane extract of Willughbeia cochinchinensis, perform column chromatography on a normal phase silica gel with n-hexane: ethyl acetate obtained in 8 fractions. From GD-D and GD-F fractions, multiple column chromatography on silica gel was performed in combination with normal and reverse phase thin-layer chromatography, four triterpene compounds as epifriedelanol (1), taraxeryl acetate (2), ambolic acid (3) and α- amyrin (4) were isolated. Their chemical structures were determined by spectrocopic methods as well as comparing with data in the literature. These compounds were isolated for the first time from Willughbeia cochinchinensis.

Determination of 3-Methoxy-4-Hydroxymandelic Acid (VMA) in Urine by Thin-Layer Chromatography

1965 ◽  
Vol 11 (10) ◽  
pp. 905-913 ◽  
Author(s):  
J S Annino ◽  
M Lipson ◽  
L A Williams
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Potassium Carbonate ◽  
Several Variables ◽  
Fast Red ◽  
Layer Chromatography

Abstract From studies of several variables, a method has been developed for the separation and quantitation of 3-methoxy-4-hydroxymandelic acid (VMA) in urine by thin-layer chromatography. The urine is pretreated with acid and Florisil, and then extracted with ethyl acetate. Thin-layer chromatography is performed on silica gel with a butanol: acetic acid:water solution. The VMA spot is located by spraying with fast red GG and potassium carbonate. After removal from the plate, maximum color is developed and quantitated by reading in a spectrophotometer at 510 mµ.

scholarly journals Development and Validation of a Method for Determination of Caffeine in Diuretic Tablets and Capsules by High-Performance Thin-Layer Chromatography on Silica Gel Plates with a Concentration Zone Using Manual Spotting and Ultraviolet Absorption Densitometry

2005 ◽  
Vol 88 (5) ◽  
pp. 1537-1543 ◽  
Author(s):  
Caitlin Sullivan ◽  
Joseph Sherma
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Ultraviolet Absorption ◽  
Concentration Zone ◽  
Layer Chromatography

Abstract A new quantitative method using silica gel high-performance thin-layer chromatography plates with channels and a concentration zone, manual application of standards and samples, development with methanol–ethyl acetate (15 + 85) mobile phase, and ultraviolet absorption densitometry is reported for the determination of caffeine in diuretic pharmaceutical preparations. Tablet and capsule products containing potassium salicylate, acetaminophen, and salicylamide as active ingredients were analyzed to test the applicability of the new method, and precision, accuracy, linearity, limits of detection and quantitation, and selectivity were validated. The milligrams of caffeine in each tablet ranged from 48.0 to 51.0, and the milligrams in each capsule from 37.9 to 40.3. Within-day precision was 1.48 and 1.78% (n = 6), and interday precision 0.723 and 1.26% (n = 5) for analysis of 2 tablets and 2 capsules, respectively. Accuracy validation of the tablet and capsule results produced errors of 1.0 and 1.9% for spiked blank analyses and 2.6 and 3.5% for standard addition analyses, respectively. A comparative study using a caffeine standard solution and a multicomponent analgesic tablet solution containing caffeine, acetaminophen, and acetylsalicylic acid showed that manual application on the concentration zone, instrumental application on the concentration zone, and instrumental application on the silica gel gave quite similar results in terms of number of theoretical plates, resolution, limit of detection, and linearity.

scholarly journals Analisis Kualitatif Kandungan Fenolik dalam Fraksi Etil Asetat dan Fraksi Metanol dari Ekstrak Kulit Jagung (Zea mays L.)

2021 ◽  
Vol 2 (1) ◽  
pp. 109
Author(s):  
Yuniarthi Dwi Suputri ◽  
Agus Dwi Ananto ◽  
Yayuk Andayani
Keyword(s):  
Qualitative Analysis ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Ethyl Acetate Fraction ◽  
Methanol Fraction ◽  
Corn Husk ◽  
Separation Result ◽  
Layer Chromatography

ABSTRAKProduksi tanaman jagung yang semakin meningkat menyebabkan peningkatan limbah kulit jagung semakin tinggi. Kulit jagung dapat dimanfaatkan dalam bidang kesehatan dan berpotensi sebagai antioksidan. Kulit jagung mengandung senyawa fenolik dan flavonoid yang dapat bertindak sebagai antioksidan. Tujuan dari penelitian ini adalah untuk mengetahui kandungan senyawa pada fenolikfraksi etil asetat dan fraksi metanol kulit jagung. Ekstraksi kulit jagung dilakukan dengan metode maserasi menggunakan pelarut etanol 96%. Fraksinasi ekstrak etanol kulit jagung dilakukan dengan fraksinasi bertingkat menggunakan pelarut metanol dan etil asetat. Kedua fraksi yang diperoleh kemudian dianalisis secara kualitatif dengan metode Kromatografi Lapis Tipis. Fase diam yang digunakan adalah plat silica gel G60F254 dan fase gerak yang digunakan adalah kombinasi eluen kloroform: etil asetat: n-butanol: asam format (5:2:2:1). Hasil pemisahan ditandai dengan munculnya noda bercak saat diamati dengan sinar UV 254 nm dan 366 nm. Nilai Rf bercak yang muncul kemudian dihitung lalu dibandingkan dengan nilai Rf literatur untuk mengidentifikasikan senyawa yang terpisah. Hasil analisis kualitatif fraksi etil asetat dan fraksi metanol kulit jagung menunjukkan keberadaan senyawa fenolik. Hasil ini ditunjukkan dengan munculnya noda bercak dengan nilai Rf 0,75 yang memiliki kemiripan dengan nilai Rf asam galat (0,76). Kata kunci : Kulit jagung; Kromatografi lapis tipis; Analisis kualitatif. ABSTRACTCorn production keeps increasing causing increasing in corn husk waste. Cornhusk can be used in medical field and have potential as an antioxidant. Cornhusk contains phenol and flavonoid metabolites that can act as antioxidants. The aim of this study is to determine the presence of phenol metabolites in ethyl acetate and methanol fraction of cornhusk. Cornhusk extraction was carried out by a maceration method using ethanol 96%. Fractionation of ethanolic cornhusk extract was carried out by liquid-liquid extraction using a separating funnel with methanol and ethyl acetate solvent. The two fractions then analyzed qualitative by Thin Layer Chromatography. The stationary phase used is silica gel G60F254. The mobile phase used is a combination of chloroform: ethyl acetate: n-butanol: formic acid (5:2:2:1). The separation result marked by the presence of stains when observed by UV 254 nm and 366 nm light. The Rf value then measured and compared to Rf value in the literature to determine the separated substance. The qualitative analysis result of corn husk ethyl acetate fraction and methanol fraction indicated the presence of phenolic metabolites. This result is showed by the presence of stain with Rf value 0,75 that has similarity with Rf value of gallic acid (0,76). Keywords : Cornhusk; Thin layer chromatography; Qualitative analysis.

Direct Fluorescent Detection of Organothiophosphorus Pesticides and Some of Their Sulfur-containing Breakdown Products After Thin Layer Chromatography

1967 ◽  
Vol 50 (5) ◽  
pp. 1088-1098
Author(s):  
Mohamed Tawfik H Ragab
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Ultraviolet Light ◽  
Congo Red ◽  
Fluorescent Detection ◽  
Bromine Vapor ◽  
Layer Chromatography ◽  
Sulfur Containing ◽  
Dark Blue

Abstract A rapid, simple, convenient, and widely applicable method for the direct fluorescent detection of organothiophosphorus pesticides and some postulated breakdown products of these compounds is presented. The coinpounds were spotted on thin layer chromatographic sheets, developed in ethyl acetate :nhexane, and made visible by exposure to bromine vapor followed by spraying with ferric chloride and 2-(o-hydroxy phenyl) benzoxazole. Of the 47 compounds tested, 32 compounds produced fluorescent blue spots vinder longwave ultraviolet light; these consisted of 25 organothiophosphorus pesticides, 5 sulfur-containing breakdown products, and phosphoric and hypophosphorous acids. A superimposed Congo red spray destroyed the fluorescence and resulted in dark blue spots against a red backgrovind. The sensitivity of this method is in the range of 0.2 to 5.0 μg, depending on the specific compound.

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