scholarly journals Lethal Effects of a Commercial Diatomaceous Earth Dust Product on Amblyomma americanum (Ixodida: Ixodidae) Larvae and Nymphs

2020 ◽  
Vol 57 (5) ◽  
pp. 1575-1581 ◽  
Author(s):  
Allan T Showler ◽  
Nicole Flores ◽  
Ryan M Caesar ◽  
Robert D Mitchel ◽  
Adalberto A Perez De León
Keyword(s):  
Diatomaceous Earth ◽  
Amblyomma Americanum ◽  
Powder Form ◽  
Model Species ◽  
Alternative Control ◽  
Lethal Effects ◽  
Development Of Resistance ◽  
Cattle Fever Tick ◽  
Laboratory Bioassays ◽  
Natural Organic Substance

Abstract With increasing development of resistance to conventional synthetic acaricides in economically and medically important ixodid species, interest in finding alternative control tactics has intensified. Laboratory bioassays were conducted, using the lone star tick, Amblyomma americanum (L.), as a model species, to assess the efficacy of a diatomaceous earth-based product, Deadzone, in comparison with a silica gel-based product, CimeXa. CimeXa is already known to be highly lethal against A. americanum larvae and nymphs. The two dust treatments were 100% effective against larvae and nymphs within 24 h after contact occurred by immersion in dry dusts and after crawling across a surface treated with the dry dusts. Contact by crawling on a dried aqueous film of the dusts, even at a concentration of 10%, was not as effective as exposure to the dusts in dry powder form. As has been demonstrated with CimeXa, it is likely that Deadzone will be capable of providing prophylactic protection of cattle from economically important one-host ixodids, such as the southern cattle fever tick, Rhipicephalus microplus (Canestrini), which vectors the causal agents of babesiosis. Diatomaceous earth can be stored indefinitely, will remain efficacious for as long as sufficient quantities remain on the substrate, it is a natural (organic) substance, and it might be amenable for limited use in environmentally protected habitats.

scholarly journals Lethal Effects of a Silica Gel + Thyme Oil (EcoVia) Dust and Aqueous Suspensions on Amblyomma americanum (Ixodida: Ixodidae) Larvae and Nymphs

2020 ◽  
Vol 57 (5) ◽  
pp. 1516-1524
Author(s):  
Allan T Showler ◽  
Bailee N Dorsey ◽  
Ryan M Caesar
Keyword(s):  
Silica Gel ◽  
Larval Mortality ◽  
Amblyomma Americanum ◽  
Life Stages ◽  
Alternative Tactics ◽  
Temporary Immersion ◽  
Thyme Oil ◽  
Aqueous Suspensions ◽  
Cattle Fever Tick ◽  
Laboratory Bioassays

Abstract Ixodids suck blood from host animals and transmit pathogens that cause important medical and veterinary diseases. As synthetic conventional acaricide resistance becomes increasingly common, alternative tactics are likely to enhance control efforts. Laboratory bioassays assessed the lethality of CimeXa, a commercial silica gel desiccant dust, and EcoVia, a commercial product containing silica gel + thyme oil, against lone star tick, Amblyomma americanum (L.), larvae and nymphs. Both life stages were completely killed by CimeXa by 24 h, and EcoVia achieved total larval mortality within 1 h when they were initially immersed in the dusts and when they crawled across treated substrate. Larvae were killed faster than nymphs. Temporary immersion of A. americanum larvae and nymphs in aqueous suspensions of the dusts were less effective than exposure to the dusts applied dry. Larval and nymphal mortality associated with crawling on dried aqueous suspensions of the products was also not as strong as when the immature life stages crawled across substrate treated with dry dusts, and EcoVia was not more efficient than CimeXa. CimeXa and EcoVia can likely be used to protect cattle in a prophylactic context because silica gel does not degrade, and EcoVia might be effective at eliminating ticks that are feeding on cattle as well. We suggest that the two dust products be assessed for efficacy against other ixodid species, such as the southern cattle fever tick, Rhipicephalus microplus (Canestrini), which transmits the causal agents of babesiosis to cattle.

scholarly journals Evaluation of a Greek Diatomaceous Earth for Stored Product Insect Control and Techniques That Maximize Its Insecticidal Efficacy

2020 ◽  
Vol 10 (18) ◽  
pp. 6441
Author(s):  
Georgia V. Baliota ◽  
Christos G. Athanassiou
Keyword(s):  
Diatomaceous Earth ◽  
Sitophilus Oryzae ◽  
Rhyzopertha Dominica ◽  
Insect Control ◽  
Stored Products ◽  
Cryptolestes Ferrugineus ◽  
Wide Range ◽  
Insecticidal Efficacy ◽  
Confused Flour Beetle ◽  
Laboratory Bioassays

Laboratory bioassays were conducted to evaluate the insecticidal efficacy of a diatomaceous earth deposit from Greece, for a wide range of stored product insects. In this context, populations of five different insect species, Tribolium confusum Jacquelin DuVal, the confused flour beetle; Sitophilus oryzae (L.), the rice weevil; Rhyzopertha dominica (F.), the lesser grain borer; Oryzaephilus surinamensis (L.), the sawtoothed grain beetle; Cryptolestes ferrugineus (Stephens), the rusty grain beetle, which cover a major spectrum of insects species of stored products worldwide, were used in the bioassays. The different treatment of diatomaceous earth (DE) rocks (grinding, diatomaceous enrichment, powder granulometry) led to the creation of five types of formulations (namely DE1, DE2, DE3, DE5 and DE6) that exhibited significant fluctuations in their insecticidal efficacy when applied on wheat. In general, some of the modified formulations were found to be very effective against species such as R. dominica and T. confusum that may be difficult to control at the current labeled doses of commercial DE formulations. Overall, our data clearly indicate that this specific Greek deposit has considerable insecticidal properties, which can be further utilized in designing commercial formulations for insect control at the postharvest stages of durable agricultural commodities, provided that the deposit will be modified at specific enrichment and granulometry levels.

scholarly journals Alternative control ofAethina tumidaMurray (Coleoptera: Nitidulidae) with lime and diatomaceous earth

Apidologie ◽  
2009 ◽  
Vol 40 (5) ◽  
pp. 535-548 ◽  
Author(s):  
Sven Buchholz ◽  
Katharina Merkel ◽  
Sebastian Spiewok ◽  
Jeff S. Pettis ◽  
Michael Duncan ◽  
...  
Keyword(s):  
Diatomaceous Earth ◽  
Alternative Control

scholarly journals Diatomaceous earth used against insect pests, applied alone or in combination with Metarhizium anisopliae and Beauveria bassiana

2014 ◽  
Vol 54 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Yasaman Shafighi ◽  
Masumeh Ziaee ◽  
Yobert Ghosta
Keyword(s):  
Beauveria Bassiana ◽  
Tribolium Castaneum ◽  
Metarhizium Anisopliae ◽  
Diatomaceous Earth ◽  
Insect Pests ◽  
Rhyzopertha Dominica ◽  
Continuous Darkness ◽  
Resistant Species ◽  
Insecticidal Efficacy ◽  
Laboratory Bioassays

Abstract Laboratory bioassays were conducted to assess the insecticidal efficacy of the formulation SilicoSec® used alone or in combination with isolates of entomopathogenic fungi, Metarhizium anisopliae (Metschinkoff) Sorokin and Beauveria bassiana (Balsamo) Vuillemin. SilicoSec® is a commercial diatomaceous earth (DE) formulation. Wheat was treated with 200 mg/kg of DE, 400 mg/kg of each isolates alone or a combination of them, against Tribolium castaneum Herbst, Rhyzopertha dominica (F.), and Oryzaephilus surinamensis L. The experiments were carried out at 27±1°C and 65±5% relative humidity (RH) in continuous darkness. The pathogenicity of all isolates was significantly low even after 7 days of exposure, with the exception of R. dominica. The isolates were virulent to the beetles, but the efficacy of the isolates was enhanced in combination with the DE. Tribolium castaneum was the most resistant species, followed by R. dominica. The findings indicated that the addition of the DE to the isolates increased the pathogenicity especially at the highest exposure interval. The addition of DE may provide satisfactory control of the insect-pests of stored products.

scholarly journals Effects of Formic Acid on Amblyomma americanum (Ixodida: Ixodidae) Larvae and Nymphs

2020 ◽  
Vol 57 (4) ◽  
pp. 1184-1192
Author(s):  
Allan T Showler ◽  
Bailee N Dorsey ◽  
Ryan M Caesar
Keyword(s):  
Formic Acid ◽  
Varroa Destructor ◽  
Direct Contact ◽  
Acid Treatment ◽  
Blood Feeding ◽  
Amblyomma Americanum ◽  
Alternative Control ◽  
Temporary Immersion ◽  
Varroa Mites

Abstract Ixodids are blood-feeding ectoparasitic vectors of many disease agents that infect humans, livestock, and wild animals. As ixodid resistance to conventional synthetic acaricides becomes increasingly problematic, natural products are receiving greater attention as possible alternative control tactics. Formic acid, produced by ants, is a commercially available product for fumigating varroa mites, Varroa destructor Anderson & Trueman, infesting honey bee, Apis mellifera L., hives, and it has been reported to repel ixodids. Lone star tick, Amblyomma americanum (L.), larvae and nymphs were used as a model ixodid to investigate deterrent, repellent, and lethal effects of formic acid as a fumigant and contact toxin in vitro in the laboratory. Although formic acid failed to deter or repel A. americanum, it was highly toxic as a fumigant to larvae at a 1% concentration even when exposure was limited to 5 min. Contact by crawling on wet, moist, and dry treated substrates under ventilated conditions causes >90% mortality to larvae in 5% formic acid concentration treatments within 30–120 min, and temporary immersion killed ≈60% of the larvae by 24 h after they were removed from the 5% formic acid treatment solution. Substantial nymphal mortality occurred after 1–1.5 h following exposure to substrate treated with the 10% concentration and immersion killed ≈45% of the nymphs. It appears that formic acid volatiles are more lethal to A. americanum immatures than direct contact with the external integument.

scholarly journals Bio-Efficacy of Diatomaceous Earth, Household Soaps, and Neem Oil against Spodoptera frugiperda (Lepidoptera: Noctuidae) Larvae in Benin

Insects ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 18
Author(s):  
Crépin T. S. Aniwanou ◽  
Antonio A. C. Sinzogan ◽  
Jean M. Deguenon ◽  
Rachidatou Sikirou ◽  
David A. Stewart ◽  
...  
Keyword(s):  
Spodoptera Frugiperda ◽  
Diatomaceous Earth ◽  
Cost Benefit Analysis ◽  
Cost Benefit ◽  
Current Control ◽  
Field Conditions ◽  
Neem Oil ◽  
Development Of Resistance ◽  
Maize Grain ◽  
Lepidoptera Noctuidae

Spodoptera frugiperda was first reported in Africa in 2016 and has since become a serious threat to maize/cereal production on the continent. Current control of the pest relies on synthetic chemical insecticides, which can negatively impact the environment and promote the development of resistance when used indiscriminately. Therefore, great attention is being paid to the development of safer alternatives. In this study, several biorational products and a semi-synthetic insecticide were evaluated. Two household soaps (“Palmida” and “Koto”) and a detergent (“So Klin”) were first tested for their efficacy against the larvae under laboratory conditions. Then, the efficacy of the most effective soap was evaluated in field conditions, along with PlantNeem (neem oil), Dezone (diatomaceous earth), and Emacot 19 EC (emamectin benzoate), in two districts, N’Dali and Adjohoun, located, respectively, in northern and southern Benin. The soaps and the detergent were highly toxic t second-instar larvae with 24 h lethal concentrations (LC50) of 0.46%, 0.44%, and 0.37% for So Klin, Koto, and Palmida, respectively. In field conditions, the biorational insecticides produced similar or better control than Emacot 19 EC. However, the highest maize grain yields of 7387 and 5308 kg/ha were recorded, respectively, with Dezone (N’Dali) and Emacot 19 EC (Adjohoun). A cost-benefit analysis showed that, compared to an untreated control, profits increased by up to 90% with the biorational insecticides and 166% with Emacot 19 EC. Therefore, the use of Palmida soap at 0.5% concentration, neem oil at 4.5 L/ha, and Dezone at 7.5 kg/ha could provide an effective, environmentally friendly, and sustainable management of S. frugiperda in maize.

scholarly journals Efficacy of Novaluron + Pyriproxyfen (Tekko Pro) Insect Growth Regulators Against Amblyomma americanum (Acari: Ixodidae), Rhipicephalus (Boophilus) annulatus, Rhipicephalus (Boophilus) microplus, and Rhipicephalus sanguineus

2019 ◽  
Vol 56 (5) ◽  
pp. 1338-1345 ◽  
Author(s):  
Allan T Showler ◽  
William A Donahue ◽  
Jessica L Harlien ◽  
Michael W Donahue ◽  
Bret E Vinson ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Metabolic Activity ◽  
Residual Activity ◽  
Rhipicephalus Sanguineus ◽  
Amblyomma Americanum ◽  
Boophilus Microplus ◽  
Insect Growth Regulators ◽  
Insect Growth ◽  
Boophilus Annulatus ◽  
Cattle Fever Tick

Abstract Ixodids are globally distributed pests that transmit many disease agents. Increasing resistance to conventional acaricides raises the need for alternative tactics. Novaluron and pyriproxyfen are insect growth regulators (IGRs) that have variable potencies against acarines. We conducted in vitro and in vivo experiments to assess novaluron + pyriproxyfen (marketed as Tekko Pro) against four ixodid species. Laboratory assays on the brown dog tick, Rhipicephalus sanguineus (Latreille) (Ixodida: Ixodidae), and the lone star tick, Amblyomma americanum (L.) (Ixodida: Ixodidae), reduced metabolic activity in larvae and nymphs. Concentrations of novaluron + pyriproxyfen dried on filter paper impeded molting of larval R. sanguineus (less effective against nymphs). Molting A. americanum larvae were reduced by >95% using 4 and 8 µg/cm2 eliminated molting; nymphal molting was reduced but not halted even at 16 µg/cm2. On calves, novaluron + pyriproxyfen stopped larval A. americanum metabolic function 1 d post-treatment and larvae did not molt. When larvae were released 30 d after treatment, metabolic activity was reduced by 95% and molting was reduced by 94%. Southern cattle fever tick, Rhipicephalus (Boophilus) microplus (Canestrini) (Ixodida: Ixodidae), larvae released 1 d after treatment on calves were 99% prevented from reaching adulthood. The treatment did not interfere with larval development when larvae were released 52 d after treatment. The cattle fever tick, Rhipicephalus (Boophilus) annulatus (Say) (Ixodida: Ixodidae), failed to reach adulthood when larvae were released on calves a day after treatment (residual activity was not assessed for R. annulatus). These IGRs, and possibly others, offer an alternative to conventional acaricides for ixodid control on cattle.

Contact micropredation may play a more important role than exotoxicity does in the lethal effects of Karlodinium australe blooms: Evidence from laboratory bioassays

Harmful Algae ◽  
2020 ◽  
Vol 99 ◽  
pp. 101926
Author(s):  
Xiaoying Song ◽  
Zhangxi Hu ◽  
Lixia Shang ◽  
Chui Pin Leaw ◽  
Po Teen Lim ◽  
...  
Keyword(s):  
Lethal Effects ◽  
Laboratory Bioassays

scholarly journals Effects of Silica-Based CimeXa and Drione Dusts Against Lone Star Tick (Ixodida: Ixodidae) on Cattle

2019 ◽  
Author(s):  
Allan T Showler ◽  
Jessica L Harlien
Keyword(s):  
Egg Production ◽  
Life Stage ◽  
Amblyomma Americanum ◽  
Boophilus Microplus ◽  
Ixodid Ticks ◽  
Life Stages ◽  
Lone Star Tick ◽  
Desiccant Dust ◽  
Cattle Fever Tick ◽  
Calf Skin

Abstract Ixodid ticks vector pathogens of humans, livestock, and wildlife, and occur in tropical and temperate regions worldwide. We used the lone star tick, Amblyomma americanum (L.) (Ixodida: Ixodidae), as a model for other ixodid species, including the southern cattle fever tick, Rhipicephalus (Boophilus) microplus (Canestrini) (Ixodida: Ixodidae). We assessed the lethality of CimeXa, an inert silica-based desiccant dust, and Drione, a silica-based desiccant dust with pyrethrin and piperonyl butoxide (PBO), against A. americanum larvae and nymphs on calves. CimeXa was highly lethal to larvae released onto shaved calf skin 72–96 h previously, and moderately toxic to nymphs. It did not affect immature life stages treated after they began feeding because desiccation was offset by ingestion of blood. Being inert, CimeXa can protect cattle from infestation before feeding occurs for a potentially indefinite period of time. Drione killed immatures within 24 h when released on treated skin and when applied to feeding ixodids because of the pyrethrin and PBO (PBO will also reduce resistance to pyrethrin if it develops). When CimeXa was applied to calves with the hair intact, ≈90% and ≈70% of A. americanum larvae and nymphs, respectively, were killed within 24 h and Drione killed 100% of each life stage. Drione also prevented and drastically reduced egg production and hatching. While CimeXa protects against infestation by immatures, Drione will also control ticks that are feeding. When pyrethrin and PBO in Drione eventually degrade, the silica aerogel carrier can continue to protect against infestation indefinitely.

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