scholarly journals Visualization of Arabidopsis Root System Architecture in 3D by Refraction-Contrast X-Ray Micro-Computed Tomography

Microscopy ◽  
2021 ◽  
Author(s):  
Tomofumi Kurogane ◽  
Daisuke Tamaoki ◽  
Sachiko Yano ◽  
Fumiaki Tanigaki ◽  
Toru Shimazu ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Root System ◽  
System Architecture ◽  
Root Systems ◽  
Root System Architecture ◽  
Field Of View ◽  
Imaging Method ◽  
Wide Field ◽  
Crop Species ◽  
X Ray

Abstract Plant roots change their morphological traits in order to adapt themselves to different environmental conditions, resulting in alteration of the root system architecture. To understand this mechanism, it is essential to visualize morphology of the entire root system. To reveal effects of long-term alteration of gravity environment on root system development, we have performed an experiment in the International Space Station using Arabidopsis plants and obtained dried root systems grown in rockwool slabs. X-ray computed tomography (CT) technique using industrial X-ray scanners has been introduced to visualize root system architecture of crop species grown in soil in 3D non-invasively. In the case of the present study, however, root system of Arabidopsis is composed of finer roots compared with typical crop plants and rockwool is also composed of fibers having similar dimension to that of the roots. A higher spatial resolution imaging method is required for distinguishing roots from rockwool. Therefore, in the present study, we tested refraction-contrast X-ray micro-CT using coherent X-ray optics available at the beamline of the synchrotron radiation facility SPring-8 for bio-imaging. We have found that wide field of view but with low resolution obtained at the experimental Hutch 3 of this beamline provided an overview map of the root systems, while narrow field of view but with high resolution obtained at the experimental Hutch 1 provided extended architecture of the secondary roots, by clear distinction between roots and individual rockwool fibers, resulting in successful tracing of these roots from their basal regions.

scholarly journals Complementary Phenotyping of Maize Root Architecture by Root Pulling Force and X-Ray Computed Tomography

2021 ◽  
Author(s):  
Mon-Ray Shao ◽  
Ni Jiang ◽  
Mao Li ◽  
Anne Howard ◽  
Kevin Lehner ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Root System ◽  
System Architecture ◽  
Abiotic Stress Tolerance ◽  
Root System Architecture ◽  
Maize Root ◽  
Crop Species ◽  
X Ray ◽  
X Ray Computed ◽  
Pulling Force

ABSTRACTThe root system is critical for the survival of nearly all land plants and a key target for improving abiotic stress tolerance, nutrient accumulation, and yield in crop species. Although many methods of root phenotyping exist, within field studies one of the most popular methods is the extraction and measurement of the upper portion of the root system, known as the root crown, followed by trait quantification based on manual measurements or 2D imaging. However, 2D techniques are inherently limited by the information available from single points of view. Here, we used X-ray computed tomography to generate highly accurate 3D models of maize root crowns and created computational pipelines capable of measuring 71 features from each sample. This approach improves estimates of the genetic contribution to root system architecture, and is refined enough to detect various changes in global root system architecture over developmental time as well as more subtle changes in root distributions as a result of environmental differences. We demonstrate that root pulling force, a high-throughput method of root extraction that provides an estimate of root biomass, is associated with multiple 3D traits from our pipeline. Our combined methodology can therefore be used to calibrate and interpret root pulling force measurements across a range of experimental contexts, or scaled up as a stand-alone approach in large genetic studies of root system architecture.

scholarly journals Quantifying the impact of soil compaction on root system architecture in tomato (Solanum lycopersicum) by X-ray micro-computed tomography

2012 ◽  
Vol 110 (2) ◽  
pp. 511-519 ◽  
Author(s):  
Saoirse R. Tracy ◽  
Colin R. Black ◽  
Jeremy A. Roberts ◽  
Craig Sturrock ◽  
Stefan Mairhofer ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Root System ◽  
Solanum Lycopersicum ◽  
System Architecture ◽  
Soil Compaction ◽  
Root System Architecture ◽  
Micro Computed Tomography ◽  
X Ray ◽  
The Impact

Quantifying the effect of soil moisture content on segmenting root system architecture in X-ray computed tomography images

2013 ◽  
Vol 370 (1-2) ◽  
pp. 35-45 ◽  
Author(s):  
Susan Zappala ◽  
Stefan Mairhofer ◽  
Saoirse Tracy ◽  
Craig J. Sturrock ◽  
Malcolm Bennett ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Soil Moisture ◽  
Moisture Content ◽  
Root System ◽  
System Architecture ◽  
Soil Moisture Content ◽  
Root System Architecture ◽  
X Ray ◽  
Computed Tomography Images ◽  
X Ray Computed

scholarly journals X-Ray Computed Tomography Reveals the Response of Root System Architecture to Soil Texture

2016 ◽  
Vol 171 (3) ◽  
pp. 2028-2040 ◽  
Author(s):  
Eric D. Rogers ◽  
Daria Monaenkova ◽  
Medhavinee Mijar ◽  
Apoorva Nori ◽  
Daniel I. Goldman ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Root System ◽  
Soil Texture ◽  
System Architecture ◽  
Root System Architecture ◽  
X Ray ◽  
X Ray Computed

scholarly journals Complementary Phenotyping of Maize Root System Architecture by Root Pulling Force and X-Ray Imaging

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
M. R. Shao ◽  
N. Jiang ◽  
M. Li ◽  
A. Howard ◽  
K. Lehner ◽  
...  
Keyword(s):  
Root System ◽  
System Architecture ◽  
Abiotic Stress Tolerance ◽  
3D Models ◽  
Developmental Time ◽  
Root System Architecture ◽  
Maize Root ◽  
Crop Species ◽  
X Ray ◽  
Pulling Force

The root system is critical for the survival of nearly all land plants and a key target for improving abiotic stress tolerance, nutrient accumulation, and yield in crop species. Although many methods of root phenotyping exist, within field studies, one of the most popular methods is the extraction and measurement of the upper portion of the root system, known as the root crown, followed by trait quantification based on manual measurements or 2D imaging. However, 2D techniques are inherently limited by the information available from single points of view. Here, we used X-ray computed tomography to generate highly accurate 3D models of maize root crowns and created computational pipelines capable of measuring 71 features from each sample. This approach improves estimates of the genetic contribution to root system architecture and is refined enough to detect various changes in global root system architecture over developmental time as well as more subtle changes in root distributions as a result of environmental differences. We demonstrate that root pulling force, a high-throughput method of root extraction that provides an estimate of root mass, is associated with multiple 3D traits from our pipeline. Our combined methodology can therefore be used to calibrate and interpret root pulling force measurements across a range of experimental contexts or scaled up as a stand-alone approach in large genetic studies of root system architecture.

scholarly journals High-throughput three-dimensional visualization of root system architecture of rice using X-ray computed tomography

Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Shota Teramoto ◽  
Satoko Takayasu ◽  
Yuka Kitomi ◽  
Yumiko Arai-Sanoh ◽  
Takanari Tanabata ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Root System ◽  
High Throughput ◽  
System Architecture ◽  
Three Dimensional ◽  
Root System Architecture ◽  
X Ray ◽  
X Ray Computed

In Situ Visualization and Quantification of Three-Dimensional Root System Architecture and Growth Using X-Ray Computed Tomography

2014 ◽  
Vol 13 (8) ◽  
pp. vzj2014.03.0024 ◽  
Author(s):  
Nicolai Koebernick ◽  
Ulrich Weller ◽  
Katrin Huber ◽  
Steffen Schlüter ◽  
Hans-Jörg Vogel ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Root System ◽  
System Architecture ◽  
Three Dimensional ◽  
Root System Architecture ◽  
X Ray ◽  
In Situ Visualization ◽  
X Ray Computed

scholarly journals Response of Mungbean Root System Architecture to Phosphorus Application Methods

Proceedings ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 173
Author(s):  
Vijaya Singh ◽  
Marisa Collins ◽  
Colin Andrew Douglas ◽  
Michael Bell
Keyword(s):  
Root System ◽  
System Architecture ◽  
Root Systems ◽  
Root System Architecture ◽  
Grain Legumes ◽  
Coarse Root ◽  
Root Parameters ◽  
P Application ◽  
Application Methods ◽  
Phosphorus Application

In recent years phosphorus application methods have become an important management strategy for optimising the uptake of the immobile nutrient phosphorus (P). Root system architecture (RSA) could play a particularly important role in the uptake of P by grain legumes, due to their relatively coarse root systems. The objective of this study was to understand the response of mungbean root systems to P application methods. Four mungbean varieties were grown in purpose-built soil filled root chambers that received five P application methods. Phosphorus treatments consisted of a control (no application of P) compared with 30 mg P/kg soil throughout the soil volume (high P treatment) or restricted to 10cm deep layers in the topsoil or in a layer from 20-30cm deep. A fifth treatment consisted of the same amount of P as applied in deeper dispersed layer applied in a concentrated band at 25cm depth. After 50 days of growth, plant were destructively harvested and shoot and root parameters were measured. Mungbean varieties responded differently to P application methods, with Jade and Berken varieties showing greater root proliferation at depth and greater shoot growth in response to banded and deeper dispersed P applications, relative to the late maturing variety Putland. Shallow dispersed P and the no-P control both resulted in poor root growth in all the genotypes except Celera II, which did not respond to P application from any placement strategy. Results suggest that P application strategies may need to vary with variety to maximize the uptake of P.

Sign in / Sign up

Export Citation Format

Share Document