Effect of Zinc Source and Picolinic Acid on 65Zn Uptake in an in Vitro Continuous-Flow Perfusion System for Pig and Poultry Intestinal Segments

1987 ◽  
Vol 117 (10) ◽  
pp. 1704-1707 ◽  
Author(s):  
Dale A. Hill ◽  
Ernest R. Peo ◽  
Austin J. Lewis
Keyword(s):  
Continuous Flow ◽  
Picolinic Acid ◽  
Perfusion System ◽  
Zinc Source ◽  
Flow Perfusion ◽  
65Zn Uptake ◽  
Intestinal Segments

scholarly journals Dynamic Seeding and in Vitro Culture of Hepatocytes in a Flow Perfusion System

2000 ◽  
Vol 6 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Stephen S. Kim ◽  
Cathryn A. Sundback ◽  
Satoshi Kaihara ◽  
Mark S. Benvenuto ◽  
Byung-Soo Kim ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Perfusion System ◽  
Flow Perfusion ◽  
Culture Of Hepatocytes

PERFUSION OF OVARIES IN VITRO AND IN VIVO

1972 ◽  
Vol 68 (2_Supplb) ◽  
pp. S285-S309 ◽  
Author(s):  
Kurt Ahrén ◽  
Per Olof Janson ◽  
Gunnar Selstam
Keyword(s):  
Perfusion System ◽  
Perfusion Technique ◽  
Preliminary Results ◽  
Advantages And Disadvantages

ABSTRACT This paper discusses in vivo and in vitro ovarian perfusion systems described so far in the literature. The interest is not focussed primarily on the results of these studies but rather on the advantages and disadvantages of the techniques and methods used. Another part of the paper summarizes the points which are most important, in our opinion, to take into consideration when developing an in vitro perfusion technique of the ovary. The last part of the paper gives a description of and some preliminary results from an in vitro perfusion system of the rabbit ovary which is under development in this laboratory.

Apoprotein C effect on triglyceride transport in tandem-perfused rat hind end and liver

1984 ◽  
Vol 247 (3) ◽  
pp. G305-G310
Author(s):  
W. J. Kortz ◽  
J. R. Nashold ◽  
M. R. Greenfield ◽  
H. Hilderman ◽  
S. H. Quarfordt
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Fatty Acyl ◽  
Liver Fat ◽  
Molar Ratio ◽  
Perfusion System ◽  
In Vitro Perfusion ◽  
Arterial Inflow ◽  
High Concentrations

The metabolism of double-labeled triglyceride in a synthetic emulsion was defined in an in vitro perfusion system of rat hind end and liver described previously [Am. J. Physiol. 245 (Gastrointest. Liver Physiol. 8): G106-G112, 1983]. The metabolism of [3H]glycerol-[14C]triolein was defined in the absence of added apoproteins and with additions of human CII and both CII and CIII. Without apoprotein, a pronounced lipolysis of the triglyceride was recognized by high concentrations of radiolabeled glycerol and free fatty acid in the perfusate. The removal of an aliquot of hind-end venous effluent 5 min after adding the labeled triglyceride emulsion to the arterial inflow demonstrated a brisk lipolysis of the substrate when incubated outside the perfusion system. The addition of CII protein to the emulsion before its introduction into the tandem system eliminated perfusate lipolysis, both within the perfusion system and in incubations of aliquots withdrawn from the system. Intravascular lipolysis was not seen with triglyceride emulsions containing both CII and CIH or when an aliquot of hind-end venous effluent was incubated with triglycerides that had not been exposed to the perfusion system. The intravascular lipolysis observed for the [14C]triglyceride added to the tandem system without apoproteins was associated with relatively greater recoveries of 14C-fatty acyl in liver, fat, and muscle and relatively greater recoveries of 14CO2 than when CII alone or both CII and CIII were added with the triglyceride. The addition of CIII to CII in a 1:1 molar ratio increased the recovery of 14C-fatty acyl in muscle and the recovery as 14CO2.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Transcriptome comparisons of in vitro intestinal epithelia grown under static and microfluidic gut-on-chip conditions with in vivo human epithelia

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kornphimol Kulthong ◽  
Guido J. E. J. Hooiveld ◽  
Loes Duivenvoorde ◽  
Ignacio Miro Estruch ◽  
Victor Marin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Culture Conditions ◽  
Gene Set Enrichment Analysis ◽  
Shear Stresses ◽  
Static Culture ◽  
Dynamic Culture ◽  
Intestinal Segments ◽  
On Chip

AbstractGut-on-chip devices enable exposure of cells to a continuous flow of culture medium, inducing shear stresses and could thus better recapitulate the in vivo human intestinal environment in an in vitro epithelial model compared to static culture methods. We aimed to study if dynamic culture conditions affect the gene expression of Caco-2 cells cultured statically or dynamically in a gut-on-chip device and how these gene expression patterns compared to that of intestinal segments in vivo. For this we applied whole genome transcriptomics. Dynamic culture conditions led to a total of 5927 differentially expressed genes (3280 upregulated and 2647 downregulated genes) compared to static culture conditions. Gene set enrichment analysis revealed upregulated pathways associated with the immune system, signal transduction and cell growth and death, and downregulated pathways associated with drug metabolism, compound digestion and absorption under dynamic culture conditions. Comparison of the in vitro gene expression data with transcriptome profiles of human in vivo duodenum, jejunum, ileum and colon tissue samples showed similarities in gene expression profiles with intestinal segments. It is concluded that both the static and the dynamic gut-on-chip model are suitable to study human intestinal epithelial responses as an alternative for animal models.

scholarly journals Impact of Chronic Tetracycline Exposure on Human Intestinal Microbiota in a Continuous Flow Bioreactor Model

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 886
Author(s):  
Youngbeom Ahn ◽  
Ji Young Jung ◽  
Ohgew Kweon ◽  
Brian T. Veach ◽  
Sangeeta Khare ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Intestinal Microbiota ◽  
Continuous Flow ◽  
Antimicrobial Drug ◽  
Bioreactor Culture ◽  
Analysis Techniques ◽  
Human Intestinal Microbiota ◽  
Traditional Cultures ◽  
The Impact

Studying potential dietary exposure to antimicrobial drug residues via meat and dairy products is essential to ensure human health and consumer safety. When studying how antimicrobial residues in food impact the development of antimicrobial drug resistance and disrupt normal bacteria community structure in the intestine, there are diverse methodological challenges to overcome. In this study, traditional cultures and molecular analysis techniques were used to determine the effects of tetracycline at chronic subinhibitory exposure levels on human intestinal microbiota using an in vitro continuous flow bioreactor. Six bioreactor culture vessels containing human fecal suspensions were maintained at 37 °C for 7 days. After a steady state was achieved, the suspensions were dosed with 0, 0.015, 0.15, 1.5, 15, or 150 µg/mL tetracycline, respectively. Exposure to 150 µg/mL tetracycline resulted in a decrease of total anaerobic bacteria from 1.9 × 107 ± 0.3 × 107 down to 2 × 106 ± 0.8 × 106 CFU/mL. Dose-dependent effects of tetracycline were noted for perturbations of tetB and tetD gene expression and changes in acetate and propionate concentrations. Although no-observed-adverse-effect concentrations differed, depending on the traditional cultures and the molecular analysis techniques used, this in vitro continuous flow bioreactor study contributes to the knowledge base regarding the impact of chronic exposure of tetracycline on human intestinal microbiota.

scholarly journals ACTIVE TRANSPORT OF D-GLOUCOSE BY INTESTINAL SEGMENTS IN VITRO, OF ICTALURUS NEBULOSUS ,

1964 ◽  
Vol 126 (2) ◽  
pp. 291-301 ◽  
Author(s):  
X. J. MUSACCHIA ◽  
S. S. NEFF ◽  
D. D. WESTHOFF
Keyword(s):  
Active Transport ◽  
Ictalurus Nebulosus ◽  
Intestinal Segments

STUDIES ON ISOLATED RAT ADRENAL CELLS I. CONTINUOUS FLOW AND BATCH INCUBATIONS

1975 ◽  
Vol 78 (1) ◽  
pp. 110-121 ◽  
Author(s):  
H. E. Falke ◽  
H. J. Degenhart ◽  
G. J. A. Abeln ◽  
H. K. A. Visser ◽  
R. J. M. Croughs
Keyword(s):  
Production Rate ◽  
Continuous Flow ◽  
Response Curve ◽  
Steroid Biosynthesis ◽  
Adrenal Cells ◽  
Flow Apparatus ◽  
Lag Period ◽  
Number Of Cells ◽  
Isolated Rat

ABSTRACT A procedure for the continuous flow incubation of isolated adrenal cells is described. In this way the advantages of continuous flow incubations of adrenal tissue are combined with those of isolated adrenal cells. Suspensions of isolated adrenal cells were prepared by a modification of the collagenase method. A sigmoid dose-response curve was obtained when these cells were incubated with ACTH in batch incubations. Under these conditions (in the presence of 1 mU ACTH/ml) the corticosterone production rate remained constant during at least 240 min. This production rate was linearly related to the number of cells. Pre-incubation of the cells during 3 h resulted in an increased response to ACTH. In continuous flow incubations without ACTH the corticosterone production was negligible. With 100 μU ACTH/ml corticosterone production increased sharply after a short lag period. A maximum was reached after 60–75 min followed by a slow decrease. Cells pre-incubated in the continuous flow apparatus had a slightly diminished ACTH response without loss of affinity to ACTH. The continuous flow incubation of isolated adrenal cells offers new possibilities for the dynamic study of steroid biosynthesis in vitro. The method may also be valuable to study processes in a wide variety of other tissues.

Speed Modulation of the HeartWare HVAD to Assess In Vitro Hemocompatibility of Pulsatile and Continuous Flow Regimes in a Rotary Blood Pump

2018 ◽  
Vol 42 (9) ◽  
pp. 879-890 ◽  
Author(s):  
Jarod T. Horobin ◽  
Michael J. Simmonds ◽  
Deepika Nandakumar ◽  
Shaun D. Gregory ◽  
Geoff Tansley ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Flow Regimes ◽  
Blood Pump ◽  
Rotary Blood Pump ◽  
Speed Modulation

Effect of structural analogues on intestinal accumulation of glycine

1962 ◽  
Vol 203 (4) ◽  
pp. 634-636 ◽  
Author(s):  
Richard P. Spencer ◽  
Janet Weinstein ◽  
Arthur Sussman ◽  
Ted M. Bow ◽  
Mary Anne Markulis
Keyword(s):  
Glutaric Acid ◽  
Succinic Dehydrogenase ◽  
Acid Transport ◽  
Depressant Effect ◽  
Amino Acid Transport System ◽  
Glycine Uptake ◽  
Hydrocinnamic Acid ◽  
Structural Analogues ◽  
Intestinal Segments

Glycine uptake (1 x 10–3 m) by hamster intestinal segments in vitro was studied after 20 min of incubation both in the absence and presence of various analogues (5 x 10–3 m). A variety of chemical relatives of glycine with modifications of the —COOH, —NH2, or α-hydrogen groups or with double modifications (such as ethanol) were without effect on glycine accumulation. Thus under these conditions glycine could not be displaced by its analogues. Three compounds, however, all α-amino acids, were effective in depressing glycine uptake (allylglycine, α-phenylglycine, l-alanine). Data are presented showing that two of these inhibitors are themselves transported and hence likely competed with the amino acid transport system. α-Aminoisobutyric acid and N-methylglycine, both known to be transported by the gut, did not interfere with glycine accumulation. In addition to oxalic acid, other succinic dehydrogenase inhibitors (hydrocinnamic acid, glutaric acid, malonic acid at 5 x 10–3 m) were without major depressant effect on glycine accumulation by this system.

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