scholarly journals The transcription factor WRKY22 is required during cryo-stress acclimation in Arabidopsis shoot tips

2020 ◽  
Vol 71 (16) ◽  
pp. 4993-5009
Author(s):  
Johanna Stock ◽  
Andrea Bräutigam ◽  
Michael Melzer ◽  
Gerd Patrick Bienert ◽  
Boyke Bunk ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Osmotic Stress ◽  
Stress Responses ◽  
Shoot Tips ◽  
Primary Metabolism ◽  
Meristematic Tissue ◽  
Cellular Events ◽  
Stress Acclimation ◽  
Genes Encoding ◽  
Model Plant Arabidopsis Thaliana

Abstract Storage of meristematic tissue at ultra-low temperatures offers a mean to maintain valuable genetic resources from vegetatively reproduced plants. To reveal the biology underlying cryo-stress, shoot tips of the model plant Arabidopsis thaliana were subjected to a standard preservation procedure. A transcriptomic approach was taken to describe the subsequent cellular events which occurred. The cryoprotectant treatment induced the changes in the transcript levels of genes associated with RNA processing and primary metabolism. Explants of a mutant lacking a functional copy of the transcription factor WRKY22 were compromised for recovery. A number of putative downstream targets of WRKY22 were identified, some related to phytohormone-mediated defense, to the osmotic stress response, and to development. There were also alterations in the abundance of transcript produced by genes encoding photosynthesis-related proteins. The wrky22 mutant plants developed an open stomata phenotype in response to their exposure to the cryoprotectant solution. WRKY22 probably regulates a transcriptional network during cryo-stress, linking the explant’s defense and osmotic stress responses to changes in its primary metabolism. A model is proposed linking WRKY53 and WRKY70 downstream of the action of WRKY22.

AtfA bZIP-type transcription factor regulates oxidative and osmotic stress responses in Aspergillus nidulans

2010 ◽  
Vol 283 (3) ◽  
pp. 289-303 ◽  
Author(s):  
Anita Balázs ◽  
Imre Pócsi ◽  
Zsuzsanna Hamari ◽  
Éva Leiter ◽  
Tamás Emri ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Osmotic Stress ◽  
Aspergillus Nidulans ◽  
Stress Responses

Transcription factor WRKY46 regulates osmotic stress responses and stomatal movement independently in Arabidopsis

2014 ◽  
Vol 79 (1) ◽  
pp. 13-27 ◽  
Author(s):  
Zhong Jie Ding ◽  
Jing Ying Yan ◽  
Xiao Yan Xu ◽  
Di Qiu Yu ◽  
Gui Xin Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Osmotic Stress ◽  
Stress Responses ◽  
Stomatal Movement

The Arabidopsis AP2/ERF transcription factor RAP2.6 participates in ABA, salt and osmotic stress responses

Gene ◽  
2010 ◽  
Vol 457 (1-2) ◽  
pp. 1-12 ◽  
Author(s):  
Qiang Zhu ◽  
Jiantao Zhang ◽  
Xiaoshu Gao ◽  
Jianhua Tong ◽  
Langtao Xiao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Osmotic Stress ◽  
Stress Responses ◽  
Salt And Osmotic Stress ◽  
Erf Transcription Factor

scholarly journals The Copper Responsive Transcription Factor SPL7 Represses Key Abscisic Acid Biosynthetic Genes to Balance Growth and Drought Tolerance

2021 ◽  
Author(s):  
Lei Li ◽  
Yanzhi Yang ◽  
Jianmei Du ◽  
Yihan Tao ◽  
Chen Hao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abscisic Acid ◽  
Drought Tolerance ◽  
Stress Responses ◽  
De Novo ◽  
Balance Growth ◽  
Arabidopsis Seedling ◽  
High Copper ◽  
Squamosa Promoter Binding Protein ◽  
Genes Encoding

Plants adapt to adverse environments by turning on defense against abiotic stresses, which is mainly orchestrated by the phytohormone abscisic acid (ABA). But how ABA homeostasis is modulated to balance growth and stress responses is still largely unknown. Here we report that prior treatment of Arabidopsis seedling with high copper retardates growth but enhances draught tolerance at later stages by modulating ABA accumulation. Subsequent genetic, physiological, transcriptomic, and molecular investigations revealed that the copper responsive transcription factor SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 7 (SPL7) is a strong regulator of ABA accumulation. We showed that SPL7 is destabilized by high copper and consistently suppresses genes encoding three key oxygenases in the ABA biosynthetic pathway of land plants via binding to the GTAC copper response motifs in their promoters. These results revealed a new mechanism whereby copper availability, inversely reflected by SPL7 abundance, modulates de novo ABA biosynthesis to balance growth and drought tolerance.

scholarly journals Physiological and Molecular Osmotic Stress Responses in Three Durum Wheat (Triticum Turgidum ssp Durum) Genotypes

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 550 ◽  
Author(s):  
Salma Jallouli ◽  
Sawsen Ayadi ◽  
Simone Landi ◽  
Giorgia Capasso ◽  
Giorgia Santini ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Osmotic Stress ◽  
Durum Wheat ◽  
Stress Responses ◽  
Triticum Turgidum ◽  
Growth Traits ◽  
Glutamate Synthase ◽  
Dry Weight ◽  
Transcript Abundance ◽  
Primary Metabolism

This study aims to investigate the activities and expression of enzymes of primary metabolism and relate these data with the growth performance of three different durum wheat genotypes (Maali; YT13; and ON66) under osmotic stress. Growth traits—including plant height, dry weight (DW) and relative water content (RWC)—were measured to classify genotypes depending on their tolerance to stress. Several enzymes were investigated: Ascorbate peroxidase (APX), Glutamine Synthetase (GS), Glutamine dehydrogenase (GDH), Glutamate synthase (GOGAT), Glucose 6-phosphate dehydrogenase (G6PDH), and Phosphoenolpyruvate Carboxylase (PEPC). The expression of the cytosolic and plastidic glutamine synthetase (TaGS1 and TaGS2), high affinity nitrate transporters (TaNRT2.3) and Glutamate dehydrogenase (TaGDH) were also detected by qRT-PCR. The results indicated different growth performances among genotypes, indicating Maali and YT13 as tolerant genotypes and ON66 as a drought-susceptible variety. Data showed a decrease in PEPC and increase in APX activities under osmotic stress; a slight decrease in GS activity was observed, together with an increase in G6PDH in all genotypes; GS and NRT2 expressions changed in a similar pattern in the different genotypes. Interestingly, Maali and YT13 showed higher transcript abundance for GDH under stress compared to ON66, suggesting the implication of GDH in protective phenomena upon osmotic stress.

scholarly journals Protein Acetylation Mediated by YfiQ and CobB Is Involved in the Virulence and Stress Response ofYersinia pestis

2018 ◽  
Vol 86 (6) ◽  
Author(s):  
Wanbing Liu ◽  
Yafang Tan ◽  
Shiyang Cao ◽  
Haihong Zhao ◽  
Haihong Fang ◽  
...  
Keyword(s):  
Stress Responses ◽  
Protein Modification ◽  
Acid Resistance ◽  
Major Protein ◽  
Primary Metabolism ◽  
Protein Acetylation ◽  
Content Type ◽  
Genes Encoding ◽  
Shock Proteins

ABSTRACTRecent studies revealed that acetylation is a widely used protein modification in prokaryotic organisms. The major protein acetylation acetyltransferase YfiQ and the sirtuin-like deacetylase CobB have been found to be involved in basic physiological processes, such as primary metabolism, chemotaxis, and stress responses, inEscherichia coliandSalmonella. However, little is known about protein acetylation modifications inYersinia pestis, a lethal pathogen responsible for millions of human deaths in three worldwide pandemics. Here we found thatYp_0659andYp_1760ofY. pestisencode the major protein acetylation acetyltransferase YfiQ and the sirtuin-like deacetylase CobB, respectively, which can acetylate and deacetylate PhoP enzymaticallyin vitro. Protein acetylation impairment incobBandyfiQmutants greatly decreased bacterial tolerance to cold, hot, high-salt, and acidic environments. Our comparative transcriptomic data revealed that the strongly decreased tolerance to stress stimuli was probably related to downregulation of the genes encoding the heat shock proteins (HtpG, HslV, HslR, and IbpA), cold shock proteins (CspC and CspA1), and acid resistance proteins (HdeB and AdiA). We found that the reversible acetylation mediated by CobB and YfiQ conferred attenuation of virulence, probably partially due to the decreased expression of thepsaABCDEFoperon, which encodes Psa fimbriae that play a key role in virulence ofY. pestis. This is the first report, to our knowledge, on the roles of protein acetylation modification in stress responses, biofilm formation, and virulence ofY. pestis.

scholarly journals Osmotic Stress or Ionic Composition: Which Affects the Early Growth of Crop Species More?

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 435
Author(s):  
Agnieszka Ludwiczak ◽  
Monika Osiak ◽  
Stefany Cárdenas-Pérez ◽  
Sandra Lubińska-Mielińska ◽  
Agnieszka Piernik
Keyword(s):  
Osmotic Stress ◽  
Stress Responses ◽  
Ionic Composition ◽  
Degradation Process ◽  
Early Growth ◽  
Cultivated Plants ◽  
C3 Plants ◽  
Growth Stages ◽  
Crop Species ◽  
Ionic Salt

Salinization is a key soil degradation process. An estimated 20% of total cultivated lands and 33% of irrigated agricultural lands worldwide are affected by high salinity. Much research has investigated the influence of salt (mainly NaCl) on plants, but very little is known about how this is related to natural salinity and osmotic stress. Therefore, our study was conducted to determine the osmotic and ionic salt stress responses of selected C3 and C4 cultivated plants. We focused on the early growth stages as those critical for plant development. We applied natural brine to simulate natural salinity and to compare its effect to NaCl solution. We assessed traits related to germination ability, seedlings and plantlet morphology, growth indexes, and biomass and water accumulation. Our results demonstrate that the effects of salinity on growth are strongest among plantlets. Salinity most affected water absorption in C3 plants (28% of total traits variation), but plant length in C4 plants (17–27%). Compensatory effect of ions from brine were suggested by the higher model plants’ growth success of ca 5–7% under brine compared to the NaCl condition. However, trait differences indicated that osmotic stress was the main stress factor affecting the studied plants.

scholarly journals Molecular cloning of cellular genes encoding retinoblastoma-associated proteins: identification of a gene with properties of the transcription factor E2F.

1992 ◽  
Vol 12 (12) ◽  
pp. 5620-5631 ◽  
Author(s):  
B Shan ◽  
X Zhu ◽  
P L Chen ◽  
T Durfee ◽  
Y Yang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Mammalian Cells ◽  
Leucine Zipper ◽  
T Antigen ◽  
Transactivation Domain ◽  
Recognition Sequence ◽  
Cellular Genes ◽  
Genes Encoding ◽  
Associated Proteins ◽  
Transcription Factor E2f

The retinoblastoma protein interacts with a number of cellular proteins to form complexes which are probably crucial for its normal physiological function. To identify these proteins, we isolated nine distinct clones by direct screening of cDNA expression libraries using purified RB protein as a probe. One of these clones, Ap12, is expressed predominantly at the G1-S boundary and in the S phase of the cell cycle. The nucleotide sequence of Ap12 has features characteristic of transcription factors. The C-terminal region binds to unphosphorylated RB in regions similar to those to which T antigen binds and contains a transactivation domain. A region containing a potential leucine zipper flanked by basic residues is able to bind an E2F recognition sequence specifically. Expression of Ap12 in mammalian cells significantly enhances E2F-dependent transcriptional activity. These results suggest that Ap12 encodes a protein with properties known to be characteristic of transcription factor E2F.

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