Transcription factor WRKY46 regulates osmotic stress responses and stomatal movement independently in Arabidopsis

Abstract Storage of meristematic tissue at ultra-low temperatures offers a mean to maintain valuable genetic resources from vegetatively reproduced plants. To reveal the biology underlying cryo-stress, shoot tips of the model plant Arabidopsis thaliana were subjected to a standard preservation procedure. A transcriptomic approach was taken to describe the subsequent cellular events which occurred. The cryoprotectant treatment induced the changes in the transcript levels of genes associated with RNA processing and primary metabolism. Explants of a mutant lacking a functional copy of the transcription factor WRKY22 were compromised for recovery. A number of putative downstream targets of WRKY22 were identified, some related to phytohormone-mediated defense, to the osmotic stress response, and to development. There were also alterations in the abundance of transcript produced by genes encoding photosynthesis-related proteins. The wrky22 mutant plants developed an open stomata phenotype in response to their exposure to the cryoprotectant solution. WRKY22 probably regulates a transcriptional network during cryo-stress, linking the explant’s defense and osmotic stress responses to changes in its primary metabolism. A model is proposed linking WRKY53 and WRKY70 downstream of the action of WRKY22.

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Osmotic Stress or Ionic Composition: Which Affects the Early Growth of Crop Species More?

Agronomy ◽

10.3390/agronomy11030435 ◽

2021 ◽

Vol 11 (3) ◽

pp. 435

Author(s):

Agnieszka Ludwiczak ◽

Monika Osiak ◽

Stefany Cárdenas-Pérez ◽

Sandra Lubińska-Mielińska ◽

Agnieszka Piernik

Keyword(s):

Osmotic Stress ◽

Stress Responses ◽

Ionic Composition ◽

Degradation Process ◽

Early Growth ◽

Cultivated Plants ◽

C3 Plants ◽

Growth Stages ◽

Crop Species ◽

Ionic Salt

Salinization is a key soil degradation process. An estimated 20% of total cultivated lands and 33% of irrigated agricultural lands worldwide are affected by high salinity. Much research has investigated the influence of salt (mainly NaCl) on plants, but very little is known about how this is related to natural salinity and osmotic stress. Therefore, our study was conducted to determine the osmotic and ionic salt stress responses of selected C3 and C4 cultivated plants. We focused on the early growth stages as those critical for plant development. We applied natural brine to simulate natural salinity and to compare its effect to NaCl solution. We assessed traits related to germination ability, seedlings and plantlet morphology, growth indexes, and biomass and water accumulation. Our results demonstrate that the effects of salinity on growth are strongest among plantlets. Salinity most affected water absorption in C3 plants (28% of total traits variation), but plant length in C4 plants (17–27%). Compensatory effect of ions from brine were suggested by the higher model plants’ growth success of ca 5–7% under brine compared to the NaCl condition. However, trait differences indicated that osmotic stress was the main stress factor affecting the studied plants.

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CHOP Enhancement of Gene Transcription by Interactions with Jun/Fos AP-1 Complex Proteins

Molecular and Cellular Biology ◽

10.1128/mcb.19.11.7589 ◽

1999 ◽

Vol 19 (11) ◽

pp. 7589-7599 ◽

Cited By ~ 95

Author(s):

Mariano Ubeda ◽

Mario Vallejo ◽

Joel F. Habener

Keyword(s):

Transcription Factor ◽

Gene Transcription ◽

Stress Responses ◽

Leucine Zipper ◽

Target Genes ◽

Cellular Stress ◽

Dominant Negative ◽

Dimerization Domain ◽

Mobility Shift

ABSTRACT The transcription factor CHOP (C/EBP homologous protein 10) is a bZIP protein induced by a variety of stimuli that evoke cellular stress responses and has been shown to arrest cell growth and to promote programmed cell death. CHOP cannot form homodimers but forms stable heterodimers with the C/EBP family of activating transcription factors. Although initially characterized as a dominant negative inhibitor of C/EBPs in the activation of gene transcription, CHOP-C/EBP can activate certain target genes. Here we show that CHOP interacts with members of the immediate-early response, growth-promoting AP-1 transcription factor family, JunD, c-Jun, and c-Fos, to activate promoter elements in the somatostatin, JunD, and collagenase genes. The leucine zipper dimerization domain is required for interactions with AP-1 proteins and transactivation of transcription. Analyses by electrophoretic mobility shift assays and by an in vivo mammalian two-hybrid system for protein-protein interactions indicate that CHOP interacts with AP-1 proteins inside cells and suggest that it is recruited to the AP-1 complex by a tethering mechanism rather than by direct binding of DNA. Thus, CHOP not only is a negative or a positive regulator of C/EBP target genes but also, when tethered to AP-1 factors, can activate AP-1 target genes. These findings establish the existence of a new mechanism by which CHOP regulates gene expression when cells are exposed to cellular stress.

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Role for the Ran Binding Protein, Mog1p, in Saccharomyces cerevisiae SLN1-SKN7 Signal Transduction

Eukaryotic Cell ◽

10.1128/ec.3.6.1544-1556.2004 ◽

2004 ◽

Vol 3 (6) ◽

pp. 1544-1556 ◽

Cited By ~ 17

Author(s):

Jade Mei-Yeh Lu ◽

Robert J. Deschenes ◽

Jan S. Fassler

Keyword(s):

Signal Transduction ◽

Transcription Factor ◽

Osmotic Stress ◽

Kinase Activity ◽

Mitogen Activated Protein Kinase ◽

Osmotic Response ◽

Mitogen Activated Protein ◽

Kinase Pathway

ABSTRACT Yeast Sln1p is an osmotic stress sensor with histidine kinase activity. Modulation of Sln1 kinase activity in response to changes in the osmotic environment regulates the activity of the osmotic response mitogen-activated protein kinase pathway and the activity of the Skn7p transcription factor, both important for adaptation to changing osmotic stress conditions. Many aspects of Sln1 function, such as how kinase activity is regulated to allow a rapid response to the continually changing osmotic environment, are not understood. To gain insight into Sln1p function, we conducted a two-hybrid screen to identify interactors. Mog1p, a protein that interacts with the yeast Ran1 homolog, Gsp1p, was identified in this screen. The interaction with Mog1p was characterized in vitro, and its importance was assessed in vivo. mog1 mutants exhibit defects in SLN1-SKN7 signal transduction and mislocalization of the Skn7p transcription factor. The requirement for Mog1p in normal localization of Skn7p to the nucleus does not fully account for the mog1-related defects in SLN1-SKN7 signal transduction, raising the possibility that Mog1p may play a role in Skn7 binding and activation of osmotic response genes.

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Screening for Osmotic Stress Responses in Rice Varieties under Drought Condition

Rice Science ◽

10.1016/j.rsci.2017.04.004 ◽

2017 ◽

Vol 24 (5) ◽

pp. 253-263 ◽

Cited By ~ 18

Author(s):

Simon Swapna ◽

Korukkanvilakath Samban Shylaraj

Keyword(s):

Osmotic Stress ◽

Stress Responses ◽

Drought Condition ◽

Rice Varieties

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Transcriptional profiling of Medicago truncatula under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses

BMC Plant Biology ◽

10.1186/1471-2229-11-109 ◽

2011 ◽

Vol 11 (1) ◽

pp. 109 ◽

Cited By ~ 72

Author(s):

Daofeng Li ◽

Yunqin Zhang ◽

Xiaona Hu ◽

Xiaoye Shen ◽

Lei Ma ◽

...

Keyword(s):

Transcription Factor ◽

Salt Stress ◽

Abiotic Stress ◽

Medicago Truncatula ◽

Stress Responses ◽

Transcriptional Profiling ◽

Cbf Transcription Factor

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A MYB-related Transcription Factor from Sheepgrass, LcMYB2, Promotes Seed Germination and Root Growth under Drought Stress

10.21203/rs.2.10859/v3 ◽

2019 ◽

Author(s):

Pincang Zhao ◽

Shenglin Hou ◽

xiufang guo ◽

Junting Jia ◽

Weiguang Yang ◽

...

Keyword(s):

Transcription Factor ◽

Drought Stress ◽

Seed Germination ◽

Osmotic Stress ◽

Root Growth ◽

Genetic Manipulation ◽

Marker Genes ◽

Plant Responses ◽

Adverse Conditions ◽

Related Transcription Factor

Abstract Background Drought is one of the most serious factors limiting plant growth and production. Sheepgrass can adapt well to various adverse conditions, including drought. However, during germination, sheepgrass young seedlings are sensitive to these adverse conditions. Therefore, the adaptability of seedlings is very important for plant survival, especially in plants that inhabit grasslands or the construction of artificial grassland. Results In this study, we found a sheepgrass MYB-related transcription factor, LcMYB2 that is up-regulated by drought stress and returns to a basal level after rewatering. The expression of LcMYB2 was mainly induced by osmotic stress and was localized to the nucleus. Furthermore, we demonstrate that LcMYB2 promoted seed germination and root growth under drought and ABA treatments. Additionally, we confirmed that LcMYB2 can regulate LcDREB2 expression in sheepgrass by binding to its promoter, and it activates the expression of the osmotic stress marker genes AtDREB2A, AtLEA14 and AtP5CS1 by directly binding to their promoters in transgenic Arabidopsis. Conclusions Based on these results, we propose that LcMYB2 improves plant drought stress tolerance by increasing the accumulation of osmoprotectants and promoting root growth. Therefore, LcMYB2 plays pivotal roles in plant responses to drought stress and is an important candidate for genetic manipulation to create drought-resistant crops, especially during seed germination.

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