Evaluation of Fluorescent Light Energy for the Treatment of Acute Second-degree Burns

ABSTRACT Introduction The use of photobiomodulation has been proposed to improve wound healing for the last two decades. Recent development in photobiomodulation has led to the development of a novel biophotonic platform that utilizes fluorescent light energy (FLE) within the visible spectrum of light for healing of skin inflammation and wounds. Materials and Methods In this article, FLE was used in preliminary analysis on 18 case studies of acute second-degree burns and in a pilot study using an ex vivo human skin model. Efficacy of FLE on wound healing and tissue remodeling was evaluated by monitoring improvements in the treated tissues, assessing pain for the patients, and by performing human genome microarray analysis of FLE-treated human skin samples. Results Healing was reported for all 18 patients treated with FLE for acute second-degree burns without reported adverse effects or development of infections. Furthermore, preliminary ex vivo skin model data suggest that FLE impacts different cellular pathways including essential immune-modulatory mechanisms. Conclusions The results presented in this article are encouraging and suggest that FLE balances different stages of wound healing, which opens the door to initiating randomized controlled clinical trials for establishing the efficacy of FLE treatment in different phases of wound healing of second-degree burns.

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662 Bacterial infection and wound healing in an ex vivo human skin model

Journal of Investigative Dermatology ◽

10.1016/j.jid.2019.07.667 ◽

2019 ◽

Vol 139 (9) ◽

pp. S328 ◽

Cited By ~ 1

Author(s):

E. Pagès ◽

M. Pastore ◽

L. Rosselle ◽

A. Barras ◽

N. Skandrani ◽

...

Keyword(s):

Wound Healing ◽

Bacterial Infection ◽

Human Skin ◽

Ex Vivo ◽

Skin Model

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Development and Evaluation of a Topical Anti-Inflammatory Preparation Containing Dodonaea polyandra Extract

Journal of Pharmacy & Pharmaceutical Sciences ◽

10.18433/j38p57 ◽

2015 ◽

Vol 18 (4) ◽

pp. 578 ◽

Cited By ~ 4

Author(s):

Bradley S Simpson ◽

Xianling Luo ◽

Jiping Wang ◽

Yunmei Song ◽

David Claudie ◽

...

Keyword(s):

Phase Separation ◽

Human Skin ◽

Dosage Form ◽

Interleukin 1 ◽

In Vitro Release ◽

Skin Inflammation ◽

Skin Model ◽

Inflammation Model ◽

Anti Inflammatory

Purpose: We have previously reported that the Australian Northern Kaanju (Kuuku I’yu) medicinal plant Dodonaea polyandra has anti-inflammatory activity. This is attributed largely to the presence of clerodane diterpenoids contained within the leaf resin. We envisaged developing a topical preparation to treat indications relating to skin inflammation. However, it was unknown whether the resin could be incorporated into a suitable dosage form while retaining the therapeutic value demonstrated in previous work. Therefore, the following study was undertaken to assess parameters of safety and efficacy for a prototype formulation containing the leaf resin extracted from D. polyandra. Methods: Using the assessment criteria of optimum appearance, tactile feeling, spreadability and odour, 78 different formulations were developed. Formulation stability was assessed using a centrifugal test with preparations displaying phase separation further modified or re-formulated. A prototype formulation containing 5% w/w plant resin was selected and subjected to in vitro release studies. This was quantified through HPLC analysis using two major bioactive diterpenoids as reference. The prototype formulation was tested for efficacy in a TPA-induced acute murine skin inflammation model as well as a 3D human skin model for irritancy/toxicity (Epiderm™). Results: The prototype resin cream was a chartreuse-coloured homogenous semisolid preparation that was readily spreadable upon contact with skin with no sensation of tackiness, residual greasiness, or irritation. The optimized cream showed no phase separation after 30 min centrifugation at 825 g. In the TPA-induced inflammation model, the resin formulation significantly reduced ear thickness and interleukin-1 beta levels in mouse ear tissue. The 5% w/w resin cream formulation showed no irritancy in a 3D human skin model. Conclusions: Our results demonstrate that bioactive resin from D. polyandra can be formulated into a stable and non-irritant semi-solid dosage form and reduce parameters of acute skin inflammation in vivo. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

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Increased pro‐collagen 1, elastin, and TGF‐β1 expression by copper ions in an ex‐vivo human skin model

Journal of Cosmetic Dermatology ◽

10.1111/jocd.13186 ◽

2019 ◽

Vol 19 (6) ◽

pp. 1522-1527 ◽

Cited By ~ 4

Author(s):

Navit Ogen‐Shtern ◽

Katerina Chumin ◽

Guy Cohen ◽

Gadi Borkow

Keyword(s):

Human Skin ◽

Copper Ions ◽

Ex Vivo ◽

Skin Model ◽

Tgf Β1

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Effect of Skin Model on In Vitro Performance of an Adhesive Dermally Applied Microarray Coated with Zolmitriptan

Journal of Pharmaceutics ◽

10.1155/2018/7459124 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Mahmoud Ameri ◽

Hayley Lewis ◽

Paul Lehman

Keyword(s):

Human Skin ◽

Ex Vivo ◽

Full Thickness ◽

Artificial Membrane ◽

Skin Model ◽

Hydrophobic Membrane ◽

Thickness Skin ◽

In Vitro Performance

Franz cell studies, utilizing different human skin and an artificial membrane, evaluating the influence of skin model on permeation of zolmitriptan coated on an array of titanium microprojections, were evaluated. Full thickness and dermatomed ex vivo human skin, as well as a synthetic hydrophobic membrane (Strat-M®), were assessed. It was found that the choice of model demonstrated different absorption kinetics for the permeation of zolmitriptan. For the synthetic membrane only 11% of the zolmitriptan coated dose permeated into the receptor media, whilst for the dermatomed skin 85% permeated into the receptor. The permeation of zolmitriptan through full thickness skin had a significantly different absorption profile and time to maximum flux in comparison to the dermatomed skin and synthetic model. On the basis of these results dermatomed skin may be a better estimate of in vivo performance of drug-coated metallic microprojections.

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