Formulasi Whey Fermentasi dengan Penambahan Buah Merah (Pandanus conoideus Lam) sebagai Tabir Surya

Whey produced from cheese processing can be used as non-food products such as sunscreen by fermenting using Streptococcus thermophilus and Lactobacillus bulgaricus. Adding active ingredients such as red fruit oil which contains carotenoids can increase the effectiveness of sunscreen. This study aims to determine the formulation of fermented whey and red fruit oil usage in sunscreen. The study began with the production of fermented whey and sunscreen formulations with ratios of 0, 1:1, 1:2, and 2:1 (fermented whey: red fruit oil). Furthermore, observe the organoleptic, antioxidant, SPF, pH, and homogeneity value. Adding fermented whey and red fruit oil showed no significant effect (P>0.05) onthe organoleptic test of aroma, but significantly (P<0.05) on color and texture. The antioxidant activity value test showed significantly different results (P<0.05) with IC50 values classified as moderate to high.Determination of the SPF value of sunscreen showed significantly different results (P<0.05), but this value was classified as low protection. Although there was no significant effect on pH testing (P>0.05), the pH of sunscreen cream indicated that it was suitable for topical application. All samples showed homogeneous results and there were no coarse particles or separation between the oil and water phases. The best sunscreen formulation is 1:1 cream with the highest SPF value of 6.86 and strong antioxidant activity with an IC50 value of 56.15 g/mL. Fermented whey and red fruit oil usage as active ingredients are more effective in increasing the protection of the cream against UV rays and free radicals.

Antioxidant and Sunscreen Activity Purified Extract of Kenikir Leaves (ETDK) and Tampoi Fruit Peel Extract (EKBT) Nanocream

Background: UV lights are free radicals that can cause damage to the skin, such as redness, burning, pigmentation, and even cancer. Kenikir leaf purified extract (ETDK) and Tampoi fruit peel extract (EKBT) contain flavonoid compounds. Flavonoid compounds can be antioxidants and sunscreens due to the presence of chromophore groups that can absorb UV rays to reduce exposure to the skin. Objectives: This study aims to determine the antioxidant activity and the ability of sunscreen nano cream Purified extract of Kenikir leaves and Tampoi fruit peel extract. Material and metode: Nanocream is made with 3 ETDTK-EKBT combination formulas, II ETDK formulas, III EKBT formulas. The antioxidant activity test was carried out using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method and the sunscreen activity test using the UV-Vis spectrophotometric method to calculate the SPF value of the preparation (Mansur's method). Result: The results showed that the nano cream formula had a strong antioxidant activity with the per cent inhibition of formula I 78.28%, formula II 68.49%, and formula III 73.00%. The activity test results as a sunscreen formulation of purified extract nano cream from Kenikir leaves and nano cream from Tampoi fruit peel extract had extra protection ability with a sun-protecting factor value of 7 while the nano cream formula combined extract with an SPF value of 10 gave the maximum protection category. Conclusion: nano cream have potential as antioxidants and sunscreens.

Evaluation of the, in vitro, photoprotetive capacity of Moringa oleifera oil for its use in sunscreen formulation

Moringa oleifera Lam is an Indian plant with applications in the agricultural and medical fields. The assets development capable of increasing the efficiency of sunscreens, mainly those of plant origin, due to their natural benefits, represents an increasing demand for cosmetology. The present study aims to identify by CG-MS the constituents of the most active oil and to evaluate the photoprotective capacity of Moringa oil, and its action in sunscreen formulations. Extracts of the oils from the Moringa seeds were evaluated for the Sun Protection Factor (SPF) observing the highest result for the dichloromethane extract. This extract showed low cytotoxic potential for human fibroblasts and it was incorporated into a sunscreen. The extract increased the SPF of the sunscreen and its effect may be related to fatty acids identified by GC-MS. The results showed the benefit of Moringa oil as a vegetable active in the sunscreen formulations by increasing the SPF of sunscreens in a natural and sustainable way.

Zinc oxide-induced changes to sunscreen ingredient efficacy and toxicity under UV irradiation

Sunscreen safety and efficacy is generally evaluated based upon the properties of the individual chemicals in a formulation. However, the photostability of sunscreens has been shown to be highly dependent on the mixture of chemicals present. To better understand how sunscreen formulation influences stability, and to establish a foundation for probing the influence of zinc oxide additives, we formulated five different small-molecule based ultraviolet-filter (UV-filter) mixtures with a Sun Protection Factor (SPF) of 15. These mixtures contained active ingredients approved in either the United States or European Union and were designed to represent formulations of actual products on the market. We evaluated the photostability and toxicity of these mixtures in the absence and presence of zinc oxide after UV exposure for two hours. Changes in UV absorbance were minimal for all five small-molecule-based mixtures without zinc oxide. The presence of either micro- or nano-sized zinc oxide caused significant small-molecule photodegradation and the degraded mixtures exhibited higher levels of toxicity in embryonic zebrafish assays. This study suggests that caution must be taken when formulating sunscreens containing both zinc oxide and small-molecule UV-filters to avoid unintended consequences during use. Graphic abstract

Intermolecular Interactions and In Vitro Performance of Methyl Anthranilate in Commercial Sunscreen Formulations

In order to afford the required level of broad-spectrum photoprotection against UV-B and UV-A radiation, sunscreens must contain a combination of UV filters. It is important that any interactions between UV filters do not adversely affect their photostability nor the overall photostability of the sunscreen formulation. In this work, we explore the feasibility of using methyl anthranilate (MA) as an alternative to the photo-unstable UV-A filter, avobenzone. From the in vitro studies presented here, we conclude that MA does not provide sufficient UV-A protection on its own but that it is more photostable in formulation than avobenzone. In addition, we found that both octocrylene (OCR) and ethylhexyl methoxycinnamate (EHMC), two commonly used UV-B filters, can stabilize MA through quenching of its triplet states, as previously reported, which has a demonstrable effect in formulation. In contrast with previously reported observations for mixtures of EHMC and avobenzone, we found no evidence of [2+2] photocycloadditions taking place between EHMC and MA. This work demonstrates how a clear insight into the photophysics and photochemistry of UV filters, as well as the interactions between them, can inform formulation design to predict sunscreen performance.

EFFICACY AND SAFETY OF INNOVATIVE SUNSCREEN (E) -4- (TERT-BUTYL) -N '- ((E) -3- (4-METHOXYPHENYL) ALLYLIDENE) BENZIDRAZIDA (TBMAB)

The constant or repeated unprotected exposure to solar radiation can result in the onset of various harmful effects, such as inflammation, genetic mutation and hyperpigmentation, even than previous photoaging. The use of photoprotective formulations is one of the most effective tools to avoid this. However, most of the UV filters are unstable to UV radiation, can even permeate the skin and cause hepatotoxic effects. The aim of this study was to assess the efficacy and safety of a innovative sunscreen substance (E) -4- (tert-butyl) -N '- ((E) -3- (4-methoxyphenyl) allylidene) benzhydrazide. Methodology: The sunscreen formulation containing the innovative substance was subjected to various stress conditions: 45±2° C, 5±2° C and exposure to sunlight. These samples were evaluated in relation to its rheology, pH, viscosity, density and SPF to determine its stability. The assessment of TBMAB release from the formulation was performed in Microette equipment. Hepatoma cells (HepG2) were used to determine the TBMAB cytotoxicity. Results and Discussion: In the stability study, no significant changes were observed in the formulation. The maximum concentration of TBMAB released from the formulation was 3.63 µg.cm-2. Also, it was evident the cytotoxic effect on the concentrations well above 3.63 µg.cm-2,displaying the safety of this substance, considering HepG2 cells. Conclusion: These results demonstrate that TBMAB is a suitable innovative substance to be used in suscreens, exhibiting stability, desired SPF value and safety, considering HepG2 cells. Thus, it could be a new alternative to prevent skin cancer.