scholarly journals TMIC-54. COMPARISON OF CELLULAR FEATURES AT AUTOPSY IN GLIOBLASTOMA PATIENTS WITH STANDARD TREATMENT OF CARE AND TUMOR TREATMENT FIELDS

2019 ◽  
Vol 21 (Supplement_6) ◽  
pp. vi259-vi259
Author(s):  
Allison Lowman ◽  
Alexander Barrington ◽  
Elizabeth Cochran ◽  
Jennifer Connelly ◽  
Wade Mueller ◽  
...  
Keyword(s):  
Electric Fields ◽  
Standard Treatment ◽  
Recurrent Glioblastoma ◽  
Research Needs ◽  
Tumor Treatment ◽  
Tissue Samples ◽  
Cellular Density ◽  
Recent Clinical Trial ◽  
Hematoxylin And Eosin

Abstract BACKGROUND The FDA has approved the use of tumor treatment fields (TTFields) on patients with recurrent glioblastoma (GBM). A recent clinical trial showed that the use of TTFields along with standard treatment of care resulted in increased quality of life and survival over five years. TTFields work by delivering low intensity electric fields that disrupt GBM tumor cell division. This works to slow or even stop GBM tumor cells from dividing. METHODS Brain tissue samples were taken from thirteen GBM patients, diagnosed at autopsy. Three patients underwent standard clinical treatment and ten patients underwent TTField therapy in addition to the standard. Tissue samples were acquired from regions where enhancement was seen on the last clinical T1 plus contrast MRI prior to death. Samples were paraffin embedded and stained with hematoxylin and eosin (H&E) and Ki67. The slides were digitized, allowing analysis of the cellular features. RESULTS Using the H&E stained slides we compared cellular density between both groups. We found that there was not a noticeable difference in the nuclei density in areas of tumor between the groups. The percent of positive nuclei on the Ki67 stained slides was calculated and there was increased mitotically active cells per area of tissue in patients that did not undergo TTField therapy. CONCLUSION These findings suggest there is a difference in the cellular features in areas of tumor in patients treated with TTFields compared to those with standard treatment alone. Additional research needs to be done evaluating more patients.

scholarly journals 4-Year Long Progression-Free and Symptom-Free Survival of a Patient with Recurrent Glioblastoma Multiforme: A Case Report of the Paleolithic Ketogenic Diet (PKD) Used as a Stand-Alone Treatment After Failed Standard Oncotherapy

2020 ◽  
Author(s):  
Csaba Tóth ◽  
Andrea Dabóczi ◽  
Madhvi Chanrai ◽  
Mária Schimmer ◽  
Katalin Horváth ◽  
...  
Keyword(s):  
Case Report ◽  
Glioblastoma Multiforme ◽  
Ketogenic Diet ◽  
Standard Treatment ◽  
Cancer Survival ◽  
Recurrent Glioblastoma ◽  
Recurrent Glioblastoma Multiforme ◽  
Free Survival ◽  
Clinical Endpoints

Studies in animal models have suggested that the ketogenic diet may be effective in the treatment of cancer. However, human cohort studies on the ketogenic diet have, thus far, failed to show benefits in cancer survival or in any other hard clinical endpoints of the disease. This paper presents a case report of a patient with glioblastoma multiforme. The patient had initially been treated with standard oncotherapy including surgery, radiotherapy and chemotherapy. Despite standard treatment, the patient experienced a recurrence of the glioblastoma seven months later. Subsequently, the patient refused radiotherapy and chemotherapy and opted to use the paleolithic ketogenic diet (PKD) as a stand-alone therapy. Following the adoption of the PKD, progression of the disease has been completely halted. At the time of writing, the patient has remained in remission for 48 months, is without side-effects and experiences an excellent quality of life without the use of any drugs.

scholarly journals 38-Month Long Progression-Free and Symptom-Free Survival of a Patient With Recurrent Glioblastoma Multiforme: A Case Report of the Paleolithic Ketogenic Diet (PKD) Used As a Stand-Alone Treatment after Failed Standard Oncotherapy

2019 ◽  
Author(s):  
Csaba Tóth ◽  
Andrea Dabóczi ◽  
Madhvi Chanrai ◽  
Mária Schimmer ◽  
Zsófia Clemens
Keyword(s):  
Case Report ◽  
Glioblastoma Multiforme ◽  
Ketogenic Diet ◽  
Standard Treatment ◽  
Cancer Survival ◽  
Recurrent Glioblastoma ◽  
Recurrent Glioblastoma Multiforme ◽  
Free Survival ◽  
Clinical Endpoints

Studies in animal models have suggested that the ketogenic diet may be effective in the treatment of cancer. However, human cohort studies on the ketogenic diet have, thus far, failed to show benefits in cancer survival or in any other hard clinical endpoints of the disease. This paper presents a case report of a patient with glioblastoma multiforme. The patient had initially been treated with standard oncotherapy including surgery, radiotherapy and chemotherapy. Despite standard treatment, the patient experienced a recurrence of the glioblastoma seven months later. Subsequently, the patient refused radiotherapy and chemotherapy and opted to use the paleolithic ketogenic diet (PKD) as a stand-alone therapy. Following the adoption of the PKD, progression of the disease has been completely halted. At the time of writing, the patient has remained in remission for 38 months, is without side-effects and experiences an excellent quality of life without the use of any drugs.

scholarly journals Deep learning-based transformation of H&E stained tissues into special stains

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Kevin de Haan ◽  
Yijie Zhang ◽  
Jonathan E. Zuckerman ◽  
Tairan Liu ◽  
Anthony E. Sisk ◽  
...  
Keyword(s):  
Visual Inspection ◽  
Kidney Diseases ◽  
Periodic Acid ◽  
Needle Core Biopsy ◽  
Silver Stain ◽  
Periodic Acid Schiff ◽  
Tissue Sections ◽  
Biopsy Tissue ◽  
Hematoxylin And Eosin

AbstractPathology is practiced by visual inspection of histochemically stained tissue slides. While the hematoxylin and eosin (H&E) stain is most commonly used, special stains can provide additional contrast to different tissue components. Here, we demonstrate the utility of supervised learning-based computational stain transformation from H&E to special stains (Masson’s Trichrome, periodic acid-Schiff and Jones silver stain) using kidney needle core biopsy tissue sections. Based on the evaluation by three renal pathologists, followed by adjudication by a fourth pathologist, we show that the generation of virtual special stains from existing H&E images improves the diagnosis of several non-neoplastic kidney diseases, sampled from 58 unique subjects (P = 0.0095). A second study found that the quality of the computationally generated special stains was statistically equivalent to those which were histochemically stained. This stain-to-stain transformation framework can improve preliminary diagnoses when additional special stains are needed, also providing significant savings in time and cost.

scholarly journals A review of aluminium phosphide poisoning and a flowchart to treat it

2016 ◽  
Vol 67 (3) ◽  
pp. 183-193 ◽  
Author(s):  
Behrooz Hashemi-Domeneh ◽  
Nasim Zamani ◽  
Hossein Hassanian-Moghaddam ◽  
Mitra Rahimi ◽  
Shahin Shadnia ◽  
...  
Keyword(s):  
Developing Countries ◽  
Health Effects ◽  
Standard Treatment ◽  
Agricultural Products ◽  
Pesticide Poisoning ◽  
Modified Delphi ◽  
Aluminium Phosphide ◽  
The World

Abstract The use of pesticides such as aluminium phosphide (AlP) has increased in the recent years and improved the quantity and quality of agricultural products in a number of developing countries. The downside is that AlP causes severe chronic and acute health effects that have reached major proportions in countries such as India, Iran, Bangladesh, and Jordan. Nearly 300,000 people die due to pesticide poisoning in the world every year. Poisoning with AlP accounts for many of these deaths. Unfortunately, at the same time, there is no standard treatment for it. The aim of this article is to give a brief review of AlP poisoning and propose a treatment flowchart based on the knowledge gained so far. For this purpose we reviewed all articles on the management of AlP poisoning published from 2000 till now. Using a modified Delphi design, we have designed a handy flowchart that could be used as a guide for AlP poisoning management of patients in emergency centres.

scholarly journals ACTR-44. AUTOPSY STUDY ON THE EFFECTS OF TUMOR TREATMENT FIELDS IN RECURRENT GLIOBLASTOMA: PRELIMINARY RESULTS AND TRIAL DESIGN

2017 ◽  
Vol 19 (suppl_6) ◽  
pp. vi10-vi10
Author(s):  
Sarah Hurrell ◽  
Elizabeth Cochran ◽  
Sean McGarry ◽  
Jennifer M Connelly ◽  
Kathleen M Schmainda ◽  
...  
Keyword(s):  
Trial Design ◽  
Recurrent Glioblastoma ◽  
Tumor Treatment ◽  
Autopsy Study ◽  
Preliminary Results

Estrogen and progestin binding by cytosolic and nuclear fractions of human meningiomas

1985 ◽  
Vol 62 (5) ◽  
pp. 750-756 ◽  
Author(s):  
Robert L. Martuza ◽  
Douglas C. Miller ◽  
David T. MacLaughlin
Keyword(s):  
Control Mechanism ◽  
Hormonal Control ◽  
Binding Assays ◽  
Tissue Samples ◽  
Content Type ◽  
Cellular Density ◽  
Equilibrium Binding ◽  
Frozen Tissue ◽  
Human Meningiomas ◽  
Fine Print

✓ Frozen tissue samples were obtained from meningiomas in 42 patients. Both cytosolic and nuclear fractions were tested for estradiol and progestin binding using equilibrium binding assays. The results were correlated with the age of the patient and the histological type and cellular density of the tumor. Cytosolic estradiol binding was noted in 25 (60%) of 42 tumors, with eight (19%) of the 42 having levels over 10 femtomoles (fM)/mg protein. Nuclear estradiol binding was detected in 16 (57%) of 28 tumors, with six (21%) of the 28 having levels over 10 fM/mg protein. Cytosolic progestin binding was noted in 16 (73%) of 22 samples, with levels in nine (41%) of 22 being greater than 10 fM/mg protein. There was no correlation between the level of cytoplasmic progestin binding and either the level of cytoplasmic estradiol binding or the level of nuclear estradiol binding. In several specimens, levels of cytoplasmic progestin binding in excess of 100 fM/mg protein were found in tissues demonstrating little or no estradiol binding by either the nucleus or the cytosol. This discrepancy differs from the situation found in other hormonally responsive tissues such as breast or uterus, and suggests either a possible derangement of the normal cellular hormonal control mechanism or that the measured hormone binder is a molecule other than a classical hormone receptor.

scholarly journals NTOX-11. DISEASE OUTCOMES, TOXICITY PROFILES, AND HEALTH-RELATED QUALITY OF LIFE IN PATIENTS WITH RECURRENT GLIOBLASTOMA RECEIVING INTRA-ARTERIAL CARBOPLATIN SALVAGE THERAPY

2017 ◽  
Vol 19 (suppl_6) ◽  
pp. vi167-vi167
Author(s):  
Anna Babcock ◽  
Meghan Hultman ◽  
Patricia Bruns ◽  
Katheryn Gilliland ◽  
Amy Schrecengost ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Salvage Therapy ◽  
Recurrent Glioblastoma ◽  
Health Related Quality ◽  
Disease Outcomes ◽  
Related Quality ◽  
Health Related

scholarly journals Clinical, Radiological, Pedobarographic, and Quality of Life Outcomes of the Ponseti Treatment for Clubfoot: A Prospective Study from Iran

2021 ◽  
Author(s):  
Kamyar Vahidi ◽  
Ahmad Shamabadi ◽  
Mohammad Hossein Nabian ◽  
Fardis Vosoughi ◽  
Ramin Zargarbashi ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Standard Treatment ◽  
Daily Activities ◽  
Life Outcomes ◽  
Long Term Outcomes ◽  
First Metatarsal ◽  
Talipes Equinovarus ◽  
A Prospective Study

Abstract Background: Talipes equinovarus, also known as clubfoot, is a congenital anomaly that affects one newborn per 1000 live births. Its standard treatment strategy is the Ponseti casting management. This study aims to report the long-term outcomes of the Ponseti treatment in Iran.Methods: A prospective cohort study was enrolled to evaluate clinical outcomes, radiological results, pedobarographic measurements, and quality of life after the Ponseti treatment in patients with clubfoot who were followed for at least five years.Results: In this study, 25 clubfeet of 18 patients were included. Significant reductions in Pirani, Dimeglio, and CAP scores, improved ankle dorsiflexion, and acceptable pedobarographic indices were observed in this study. From the radiological evaluation indices, the calcaneal pitch and lateral talus-first metatarsal angles were significantly reduced. The quality of life of patients after five years of treatment was favorable, which was better in females. This study showed that the results of the Ponseti treatment remained acceptable after five years.Conclusions: The Ponseti management for clubfoot in the long term appears to maintain significant improvements. However, the rate of recurrence – albeit without disruption to daily activities - cannot be ignored.

scholarly journals The impact of music on health-related quality of life, as quantified by the SF-36: A systematic review and meta-analysis

2021 ◽  
Author(s):  
J. Matt McCrary ◽  
Eckart Altenmuller ◽  
Clara Kretschmer ◽  
Daniel S. Scholz
Keyword(s):  
Standard Treatment ◽  
Meta Analysis ◽  
Music Listening ◽  
Health Related Quality ◽  
Post Intervention ◽  
Sf 36 ◽  
Related Quality ◽  
Health Related ◽  
The Impact

Background/Objectives: Increasing evidence supports the ability of music to broadly promote wellbeing and health-related quality of life (HRQOL). However, the magnitude of music effects on HRQOL is still unclear, particularly relative to established interventions, limiting inclusion of music interventions in health policy and care. The SF-36 is the most widely used instrument to evaluate HRQOL, with broad validity in evaluating the effects of a range of interventions. This study aims to synthesize and contextualize the impact of music interventions on HRQOL, as assessed by the SF-36. Methods: MEDLINE; EMBASE; Web of Science; PsycINFO, clinicaltrials.gov, and ICTRP databases were searched on 30 July 2021. Randomized and single-group studies of music interventions which reported SF-36 data at pre- and post-intervention timepoints were included. Observational studies were excluded. The quality and certainty of evidence provided by included articles and meta-analysis results was appraised using GRADE. Inverse variance random effects meta-analyses quantified changes in SF-36 mental and physical component summary scores (respectively, MCS and PCS) pre- to post-intervention and vs. common control groups. Results: Analyses included 764 participants from 25 studies. Music interventions (music listening, 10 studies; music therapy, 7 studies; singing, 7 studies; gospel music, 1 study) significantly improved MCS (Mean difference (MD) [95% confidence interval]=3.0 [1.4, 4.6]; p<.001) and PCS (MD=1.0 [0.1, 2.0; p<.04) scores. In a subgroup (8 studies; music group, N=254; control, N=257) addition of music to standard treatment for a range of conditions significantly improved MCS scores vs. standard treatment alone (MD=3.7 [0.4, 7.1; p<.03). Effects did not vary between music listening, therapy and singing intervention types or doses (p>.12); no evidence of small study or publication biases was present in any analysis (p>.31). Music impact on MCS scores meets SF-36 minimum important difference thresholds (MD>/=3) and is within the range of established interventions. Conclusions: This study provides Moderate quality evidence that music interventions can generally be used to provide clinically meaningful improvements in HRQOL. Further study is needed to determine optimal music interventions and doses for distinct clinical and public health scenarios. Funding: Alexander von Humboldt Foundation Registration: PROSPERO (ID: CRD42021276204)

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