Separation and Quantification of Abscisic Acid and its Metabolites by High-performance Liquid Chromatography

1983 ◽  
Vol 52 (3) ◽  
pp. 407-409 ◽  
Author(s):  
S. MAPELLI ◽  
P. ROCCHI
Keyword(s):  
High Performance Liquid Chromatography ◽  
Abscisic Acid ◽  
Liquid Chromatography ◽  
High Performance

scholarly journals Bioactive Molecules for Discriminating Robinia and Helianthus Honey: High-Performance Liquid Chromatography–Electron Spray Ionization–Mass Spectrometry Polyphenolic Profile and Physicochemical Determinations

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4433
Author(s):  
Otilia Bobiş ◽  
Victoriţa Bonta ◽  
Mihaiela Cornea-Cipcigan ◽  
Gulzar Ahmad Nayik ◽  
Daniel Severus Dezmirean
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Abscisic Acid ◽  
Liquid Chromatography ◽  
High Performance ◽  
Raw Material ◽  
Bioactive Molecules ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Polyphenolic Profile

Bioactive molecules from the class of polyphenols are secondary metabolites from plants. They are present in honey from nectar and pollen of flowers from where honeybees collect the “raw material” to produce honey. Robinia pseudoacacia and Helianthus annuus are important sources of nectar for production of two monofloral honeys with specific characteristics and important biological activity. A high-performance liquid chromatography–electro spray ionization–mass spectrometry (HPLC–ESI–MS) separation method was used to determine polyphenolic profile from the two types of Romanian unifloral honeys. Robinia and Helianthus honey showed a common flavonoid profile, where pinobanksin (1.61 and 1.94 mg/kg), pinocembrin (0.97 and 1.78 mg/kg) and chrysin (0.96 and 1.08 mg/kg) were identified in both honey types; a characteristic flavonoid profile in which acacetin (1.20 mg/kg), specific only for Robinia honey, was shown; and quercetin (1.85 mg/kg), luteolin (21.03 mg/kg), kaempferol (0.96 mg/kg) and galangin (1.89 mg/kg), specific for Helianthus honey, were shown. In addition, different phenolic acids were found in Robinia and Helianthus honey, while abscisic acid was found only in Robinia honey. Abscisic acid was correlated with geographical location; the samples collected from the south part of Romania had higher amounts, due to climatic conditions. Acacetin was proposed as a biochemical marker for Romanian Robinia honey and quercetin for Helianthus honey.

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