Analysis of 3-indolylacetic acid and abscisic acid by high-performance liquid chromatography and gas-liquid chromatography

Abstract We describe a modified high-performance liquid-chromatographic method for the simultaneous analysis of carbamazepine andits biologically active metabolite, carbamazepine-10, 11-epoxide. Concentrations of both these compounds in the plasma of 35 epileptic patients receiving chronic carbamazepine therapy are presented. Concentrations of carbamazepine in plasma were related to those of carbamazepine-10, 11-epoxide (r - 0.495, P less than 0.05). Total daily doses of carbamazepine were better correlated with plasma concentrations of carbamazepine-10, 11-epoxide (r = 0.714, P less than 0.001) than of carbamazepine (r = 0.269, P greater than 0.05). Close correlations were found between results of the three assay procedures we used to measure plasma carbamazepine concentrations: high-performance liquid chromatography, gas-liquid chromatography, and enzyme immunoassay. Correlation coefficients exceeded 0.97 and regression slopes were near unity, indicating that all three procedures were individually specific for the quantification of plasma carbamazepine.

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Correlation of reversed-phase high-performance liquid chromatography and gas—liquid chromatography for fatty acid compositions of some vegetable oils

Journal of Chromatography A ◽

10.1016/0021-9673(93)87037-m ◽

1993 ◽

Vol 629 (2) ◽

pp. 237-241 ◽

Cited By ~ 14

Author(s):

Hiroaki Konishi ◽

William E. Neff ◽

Timothy L. Mounts

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Fatty Acid ◽

Vegetable Oils ◽

High Performance ◽

Reversed Phase ◽

Gas Liquid Chromatography ◽

Fatty Acid Compositions

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Abscisic acid concentration in Douglas-fir needles in relation to lifting date, cold storage, and postplanting vigor of seedlings

Canadian Journal of Forest Research ◽

10.1139/x87-066 ◽

1987 ◽

Vol 17 (5) ◽

pp. 383-387 ◽

Cited By ~ 5

Author(s):

Pasi Puttonen

Keyword(s):

Abscisic Acid ◽

Liquid Chromatography ◽

Acid Concentration ◽

High Performance ◽

Storage Conditions ◽

Gas Liquid Chromatography ◽

Light Storage ◽

C Storage ◽

Polyethylene Bags

Spring-lifted seedlings were grown in pots in the field and, after a natural fall photoperiod, exposed to three 25-day cold (+4 °C) storage treatments and two lifting times, mid-November and mid-January. The storage treatments were light storage in pots, dark storage in pots, and bareroot storage in polyethylene bags in the dark. In a second experiment, an extended fall photoperiod treatment was applied to seedlings that were then stored in pots and subjected to the same light and dark treatments above. In both experiments, needle samples were taken four times during and after the treatments for abscisic acid assay. Abscisic acid concentrations were determined using gas liquid chromatography after purification with high performance liquid chromatography. Lifting times and storage treatments did not result in statistically significant differences in abscisic acid concentrations. However, there were treatment differences in characteristics of postplanting performance. Mid-November lifting resulted in reduced survival and a greater number of days to bud flush compared with the mid-January lifting results. The extended fall photoperiod material produced similar results to the natural fall photoperiod material. The failure to detect a relationship between needle abscisic acid concentration and seedling vigor may have been due to a transitory role of abscisic acid in the storage conditions studied. The quantification method for abscisic acid is insensitive and laborious for practical seedling testing.

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