Antitumor Activity of D-Mannosamine In Vitro: Cytotoxic Effect Produced by Mannosamine in Combination with Free Fatty Acids on Human Leukemia T-Cell Lines

1985 ◽  
Keyword(s):  
Fatty Acids ◽  
T Cell ◽  
Antitumor Activity ◽  
Free Fatty Acids ◽  
Cell Lines ◽  
Cytotoxic Effect ◽  
Human Leukemia ◽  
T Cell Lines

scholarly journals Allogeneic T-cell clones able to selectively destroy Philadelphia chromosome-bearing (Ph1+) human leukemia lines can also recognize Ph1- cells from the same patient

Blood ◽  
1994 ◽  
Vol 83 (11) ◽  
pp. 3390-3402 ◽  
Author(s):  
KR Oettel ◽  
OH Wesly ◽  
MR Albertini ◽  
JA Hank ◽  
O Iliopolis ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
In Vitro Model ◽  
Target Cells ◽  
Human Leukemia ◽  
T Cell Clones ◽  
Cell Clones ◽  
Vitro Model ◽  
T Cell Lines

Immunocompetent cells in bone marrow allografts have been associated with a graft-versus-leukemia (GVL) effect. To further characterize effector mechanisms that may be involved in this GVL phenomenon, we have previously established an in vitro model to identify allogeneic T- cell clones that selectively mediate cytotoxicity against a patient's leukemic cells, but not against nonleukemic lymphocytes from the same patient. We have modified this in vitro model to test whether the Ph1 chromosome and the P210 fusion protein it controls have a detectable role in leukemia-specific recognition by allogeneic T-cell clones. In this report, T-cell lines reactive with allogeneic Ph1 chromosome- bearing (Ph1+) chronic myeloid leukemia (CML) cell lines were derived and selected to be minimally reactive with Ph1 negative (Ph1-) lymphoid lines from the same patient. However, after prolonged culture, these same T-cell lines also mediated significant destruction of the Ph1- target cells from the same patients. These T-cell lines specifically recognized cells from the allogeneic CML patient to which they were sensitized, and were not contaminated by an outgrowth of natural killer cells. Furthermore, subclones could be derived from these T-cell lines, and some of these subclones again showed selective killing of the allogeneic Ph1+ leukemia cell lines, and not of the Ph1- cell line from the same patient. Analyses of T-cell receptor (TCR) genes showed the alloreactive T-cell lines and the Ph1+ selective subclones derived from them to be of the same clonal origin. This suggests that the same T cells reacting with antigens expressed on the nonleukemic Ph1- targets can at times selectively and preferentially kill the allogeneic Ph1+ cells. As the same TCR that recognizes Ph1+ cells also can recognize the Ph1- targets, it appears that the Ph1+ chromosome does not play a detectable role in recognition by these allogeneic T-cell clones. This in vitro observation may provide a model for evaluating the relationship between GVL and graft-versus-host disease effects.

scholarly journals Allogeneic T-cell clones able to selectively destroy Philadelphia chromosome-bearing (Ph1+) human leukemia lines can also recognize Ph1- cells from the same patient

Blood ◽  
1994 ◽  
Vol 83 (11) ◽  
pp. 3390-3402 ◽  
Author(s):  
KR Oettel ◽  
OH Wesly ◽  
MR Albertini ◽  
JA Hank ◽  
O Iliopolis ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
In Vitro Model ◽  
Target Cells ◽  
Human Leukemia ◽  
T Cell Clones ◽  
Cell Clones ◽  
Vitro Model ◽  
T Cell Lines

Abstract Immunocompetent cells in bone marrow allografts have been associated with a graft-versus-leukemia (GVL) effect. To further characterize effector mechanisms that may be involved in this GVL phenomenon, we have previously established an in vitro model to identify allogeneic T- cell clones that selectively mediate cytotoxicity against a patient's leukemic cells, but not against nonleukemic lymphocytes from the same patient. We have modified this in vitro model to test whether the Ph1 chromosome and the P210 fusion protein it controls have a detectable role in leukemia-specific recognition by allogeneic T-cell clones. In this report, T-cell lines reactive with allogeneic Ph1 chromosome- bearing (Ph1+) chronic myeloid leukemia (CML) cell lines were derived and selected to be minimally reactive with Ph1 negative (Ph1-) lymphoid lines from the same patient. However, after prolonged culture, these same T-cell lines also mediated significant destruction of the Ph1- target cells from the same patients. These T-cell lines specifically recognized cells from the allogeneic CML patient to which they were sensitized, and were not contaminated by an outgrowth of natural killer cells. Furthermore, subclones could be derived from these T-cell lines, and some of these subclones again showed selective killing of the allogeneic Ph1+ leukemia cell lines, and not of the Ph1- cell line from the same patient. Analyses of T-cell receptor (TCR) genes showed the alloreactive T-cell lines and the Ph1+ selective subclones derived from them to be of the same clonal origin. This suggests that the same T cells reacting with antigens expressed on the nonleukemic Ph1- targets can at times selectively and preferentially kill the allogeneic Ph1+ cells. As the same TCR that recognizes Ph1+ cells also can recognize the Ph1- targets, it appears that the Ph1+ chromosome does not play a detectable role in recognition by these allogeneic T-cell clones. This in vitro observation may provide a model for evaluating the relationship between GVL and graft-versus-host disease effects.

scholarly journals Selective cytotoxicity of 125I-labeled monoclonal antibody T101 in human malignant T cell lines

Blood ◽  
1986 ◽  
Vol 67 (2) ◽  
pp. 429-435
Author(s):  
E Boven ◽  
T Lindmo ◽  
JB Mitchell ◽  
PA Jr Bunn
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Specific Activity ◽  
Specific Radioactivity ◽  
High Specific Activity ◽  
Tumor Localization ◽  
Cytotoxic Effects ◽  
High Specific Radioactivity ◽  
T Cell Lines

The radiolabeled anti-T cell antibody T101 can be used for specific tumor localization, but unlabeled T101 produces limited cytotoxicity in patients. We thus studied the in vitro cytotoxic effects of T101 labeled with 125I, a radionuclide known for its short-range, high- linear-energy electrons. We showed that 125I-T101 could be readily prepared at high specific activity with high immunoreactivity. Human malignant T cell lines HUT 102, MOLT-4, and HUT 78 were found to differ in the number of T65 determinants (the antigen recognized by T101) and the sensitivity to external x-ray radiation, which were of significance for the cytotoxicity of 125I-T101 in vitro. The cytotoxic effects of 125I-T101 were also found to be dose dependent and increased with exposure time under frozen conditions. As controls, unlabeled T101 had no cytotoxic effect, while free Na 125I or the 125I-labeled irrelevant antibody 9.2.27 exerted minor cytotoxicity. In HUT 102 and MOLT-4, more than 3 logs' cell killing was achieved within four weeks. Because considerable cytotoxicity was demonstrated in vitro by 125I-T101 on T65- positive malignant cells, and because low-dose 111In-T101 can be used successfully for tumor localization, future trials using 125I-T101 at high specific radioactivity may improve therapeutic results in patients with T65-positive malignancies.

scholarly journals Verotoxin A Subunit Protects Lymphocytes and T Cell Lines against X4 HIV Infection in Vitro

Toxins ◽  
2012 ◽  
Vol 4 (12) ◽  
pp. 1517-1534 ◽  
Author(s):  
Pei Shi ◽  
Beth Binnington ◽  
Darinka Sakac ◽  
Yulia Katsman ◽  
Stephanie Ramkumar ◽  
...  
Keyword(s):  
T Cell ◽  
Hiv Infection ◽  
Cell Lines ◽  
A Subunit ◽  
T Cell Lines

Expression of the CR1 Receptor on Human Leukemia-Derived CD4+ T Cell Lines

1995 ◽  
Vol 163 (1) ◽  
pp. 139-147 ◽  
Author(s):  
Dania D. Yaskanin ◽  
Frank J. Waxman
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Cd4 T Cell ◽  
Human Leukemia ◽  
T Cell Lines

OC.07.3 IN VITRO EFFECTS OF MESENCHYMAL STROMAL CELLS (MSC) ON T CELL LINES FROM CROHN'S DISEASE PATIENTS

2013 ◽  
Vol 45 ◽  
pp. S71
Author(s):  
R. Ciccocioppo ◽  
G.C. Cangemi ◽  
E. Betti ◽  
A. Gallia ◽  
V. Imbesi ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
T Cell ◽  
Crohn's Disease ◽  
Cell Lines ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
In Vitro Effects ◽  
T Cell Lines

scholarly journals CD8 T cell clones from young nonobese diabetic (NOD) islets can transfer rapid onset of diabetes in NOD mice in the absence of CD4 cells.

1996 ◽  
Vol 183 (1) ◽  
pp. 67-76 ◽  
Author(s):  
F S Wong ◽  
I Visintin ◽  
L Wen ◽  
R A Flavell ◽  
C A Janeway
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cell Lines ◽  
Nod Mice ◽  
Cd8 T Cell ◽  
Rapid Onset ◽  
Cytotoxic T Cell ◽  
Nonobese Diabetic ◽  
T Cell Lines

T cells play an important role in the pathogenesis of diabetes in the nonobese diabetic (NOD) mouse. CD8 cytotoxic T cell lines and clones were generated from the lymphocytic infiltrate in the islets of Langerhans of young (7-wk-old). NOD mice by growing them on (NOD x B6-RIP-B7-1)F1 islets. These cells proliferate specifically to NOD islets and kill NOD islets in vitro. The cells are restricted by H-2Kd, and all bear T cell antigen receptor encoded by V beta 6. When these CD8 T cell lines and clones are adoptively transferred to irradiated female NOD, young NOD-SCID, and CB17-SCID mice, diabetes occurs very rapidly, within 10 d of transfer and without CD4 T cells.

scholarly journals CD4-Independent Infection of Two CD4−/CCR5−/CXCR4+ Pre-T-Cell Lines by Human and Simian Immunodeficiency Viruses

2000 ◽  
Vol 74 (14) ◽  
pp. 6689-6694 ◽  
Author(s):  
Alessandra Borsetti ◽  
Cristina Parolin ◽  
Barbara Ridolfi ◽  
Leonardo Sernicola ◽  
Andrea Geraci ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Chemokine Receptors ◽  
Immunodeficiency Virus ◽  
Cxcr4 Coreceptor ◽  
T Cell Lines ◽  
Hiv 1

ABSTRACT The infection of CD4-negative cells by variants of tissue culture-adapted human immunodeficiency virus type 1 (HIV-1) or HIV-2 strains has been shown to be mediated by the CXCR4 coreceptor. Here we show that two in vitro-established CD4−/CCR5−/CXCR4+ human pre-T-cell lines (A3 and A5) can be productively infected by wild-type laboratory-adapted T-cell-tropic HIV-1 and HIV-2 strains in a CD4-independent, CXCR4-dependent fashion. Despite the absence of CCR5 expression, A3 and A5 cells were susceptible to infection by the simian immunodeficiency viruses SIVmac239 and SIVmac316. Thus, at least in A3 and A5 cells, one or more of the chemokine receptors can efficiently support the entry of HIV and SIV isolates in the absence of CD4. These findings suggest that to infect cells of different compartments, HIV and SIV could have evolved in vivo to bypass CD4 and to interact directly with an alternative receptor.

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