Ultrastructural Study of Rabbit Buccal Epithelial Cells and Intercellular Junction by Scanning and Transmission Electron Microscopy

1993 ◽  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Epithelial Cells ◽  
Ultrastructural Study ◽  
Intercellular Junction ◽  
Transmission Electron ◽  
Buccal Epithelial Cells

Tumor Diagnosis

1982 ◽  
Vol 40 ◽  
pp. 262-265
Author(s):  
Bruce Mackay
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Differential Diagnosis ◽  
Light Microscopy ◽  
Clinical Examination ◽  
Ultrastructural Study ◽  
Diagnostic Procedures ◽  
Specific Diagnosis ◽  
Transmission Electron ◽  
Microscopic Diagnosis

The broadest application of transmission electron microscopy (EM) in diagnostic medicine is the identification of tumors that cannot be classified by routine light microscopy. EM is useful in the evaluation of approximately 10% of human neoplasms, but the extent of its contribution varies considerably. It may provide a specific diagnosis that can not be reached by other means, but in contrast, the information obtained from ultrastructural study of some 10% of tumors does not significantly add to that available from light microscopy. Most cases fall somewhere between these two extremes: EM may correct a light microscopic diagnosis, or serve to narrow a differential diagnosis by excluding some of the possibilities considered by light microscopy. It is particularly important to correlate the EM findings with data from light microscopy, clinical examination, and other diagnostic procedures.

An Ultrastructural Study of Chronic Pyelonephritis in Macaca Arctoides

1976 ◽  
Vol 34 ◽  
pp. 310-311
Author(s):  
T.W. Smith ◽  
J.A. Roberts ◽  
B.J. Martin
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Effective Means ◽  
Chronic Pyelonephritis ◽  
Macaca Arctoides ◽  
Left Ureter ◽  
E Coli ◽  
Transmission Electron ◽  
Sizeable Number

Chronic pyelonephritis is one of the most common diseases of the kidney and accounts for a sizeable number of cases of renal insufficiency in man, however its pathogenesis requires further elucidation. Transmission electron microscopy may serve as a uniquely effective means of observing details of the nature of this disease. The present paper describes preliminary results of an ultrastructural study of chronic pyelonephritis in Macaca arctoides (stumptail monkey).The infection was induced in these experiments in a retrograde fashion by means of a unilateral catheterization of the left ureter whereby an innoculum of 10 cc of broth containing approximately 2 billion E. coli per cc and radio-opaque dye were injected under pressure (mimicing vesico-ureteric reflux).

Spermiogenesis and sperm ultrastructure of Carmyerius endopapillatus (Digenea, Gastrothylacidae), a parasite of Bos taurus in Senegal

2008 ◽  
Vol 53 (1) ◽  
Author(s):  
Momar Seck ◽  
Bernard Marchand ◽  
Cheikh Bâ
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Bos Taurus ◽  
Posterior Extremity ◽  
Sperm Ultrastructure ◽  
Lateral Expansion ◽  
Transmission Electron ◽  
Plasmic Membrane ◽  
Flagellar Rotation

AbstractUltrastructural study of spermiogenesis and of the spermatozoon of Carmyerius endopapillatus has enabled to describe some characteristics of this digenea. The intercentriolar body situated between the two striated roots and the two centrioles, presents a symmetric organization. Both external bands of this intercentriolar body are made up of a row of granules. During spermiogenesis, a flagellar rotation of 90° is described. The old spermatid does not present external ornamentations. The spermatozoon is characterized, in its anterior region, by the presence of a lateral expansion exhibiting one spinelike body. In C. endopapillatus, external ornamentations are localized only at the level where the lateral expansion appears. The posterior extremity of spermatozoon exhibits a nucleus surrounded by a plasmic membrane lacking microtubules, but presenting a small lateral expansion. This is the first species of Gastrothylacidae family studied by transmission electron microscopy.

Dipetalonema viteae: ultrastructural study on the in vitro interaction between rat macrophages and microfilariae in the presence of IgE antibody

Parasitology ◽  
1981 ◽  
Vol 82 (1) ◽  
pp. 55-62 ◽  
Author(s):  
M. A. Ouaissi ◽  
A. Haque ◽  
A. Capron
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Peritoneal Macrophages ◽  
Immune Serum ◽  
Sequence Of Events ◽  
Transmission Electron ◽  
Dipetalonema Viteae ◽  
In Vitro Interaction

SUMMARYThe in vitro interaction between rat peritoneal macrophages and Dipetalonema viteae microfilariae in the presence of amicrofilaraemic rat immune serum was studied by transmission electron microscopy. The probable sequence of events leading to the killing of D. viteae microfilaria by macrophages is as follows. (a) Rat peritoneal macrophages in the presence of amicrofilaraemic rat immune serum adhere to the parasite surface, (b) the macrophages extend their pseudopodia around the parasite, (c) the ‘lysosome-like’ granules discharge their contents on to the parasite surface, (d) the lytic activity of these products begins at the parasite surface and (e) subsequent breaking of the microfilarial cuticle occurs, exposing the parasite intracellular material.

Ultrastructural study of spermatozoa of the paddlefish, Polyodon spathula

Zygote ◽  
2005 ◽  
Vol 13 (3) ◽  
pp. 241-247 ◽  
Author(s):  
Otilia Zarnescu
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Primitive Type ◽  
Polyodon Spathula ◽  
Transmission Electron ◽  
Architectural Features

Paddlefish, Polyodon spathula, spermatozoa were examined by transmission electron microscopy. Their structure has the same characteristic architectural features as sturgeon spermatozoa. Paddlefish spermatozoa are of the primitive type and consist of a rod-shaped head, a midpiece and a long flagellum. The head is about 5.15 mm in length and contains the nucleus and an apical acrosomal complex. Inside the nucleus there are three nuclear channels that begin in the subacrosomal area and have a triple helical arrangement. An nuclear fossa is present centrally, at the posterior end of the nucleus. The midpiece contains a pair of centrioles in a perpendicular arrangement, mitochondria and a narrow cytoplasmic sleeve. The flagellum has a central axoneme with a 9+2 pattern and two lateral projections or fins.

Oviducal structure in four species of gekkonid lizard differing in parity mode and eggshell structure

1998 ◽  
Vol 10 (2) ◽  
pp. 139 ◽  
Author(s):  
J. E. Girling ◽  
A. Cree ◽  
L. J. Guillette, Jr
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Epithelial Cells ◽  
Uterine Tube ◽  
Hemidactylus Turcicus ◽  
Transmission Electron ◽  
Eggshell Structure ◽  
Scanning Electron ◽  
Parity Mode

Oviducal structure was analysed in vitellogenic females from four species of gekkonid lizard exhibiting variation in parity mode and eggshell structure: Hemidactylus turcicus (oviparous) which produces a hard, calcareous eggshell; Saltuarius wyberba (oviparous) which produces a soft, parchment-like eggshell; and Hoplodactylus maculatus and Hoplodactylus duvaucelii (both viviparous). Oviducts were analysed by light, scanning electron and transmission electron microscopy. The uterus exhibited differences among species that were directly attributable to parity mode. H. turcicus and S. wyberba (oviparous) had numerous uterine shell glands; H. maculatus and H. duvaucelii(viviparous) had very few. The uterus also exhibited differences between the two oviparous species (H. turcicusand S. wyberba) which may be related to the type of eggshell produced. Variations were noted in the staining properties of the uterine glandular and epithelial cells. The structure of the infundibulum, uterine tube, isthmus and vagina also differed among species, but differences could not be directly related to parity mode or eggshell structure. Instead, the differences may be related to how prepared the oviduct is for ovulation in individuals analysed from the different species. This study confirms, in the Gekkonidae, aspects of oviducal structure that have been associated with parity mode in other squamate taxa.

An ultrastructural study of the culture forms of Trypanosoma (Schizotrypanum) myoti

1983 ◽  
Vol 61 (12) ◽  
pp. 2807-2815 ◽  
Author(s):  
W. Brockley Paterson ◽  
Patrick T. K. Woo
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Myotis Lucifugus ◽  
Basal Bodies ◽  
Kinetoplast Dna ◽  
Glutaraldehyde Fixation ◽  
Transmission Electron ◽  
Multiple Fission ◽  
Saline Medium

Trypanosoma myoti from the bat Myotis lucifugus was cultured in a diphasic blood-agar and saline medium and examined using transmission electron microscopy. Glutaraldehyde fixation revealed a pattern of kinetoplast DNA condensation in the epimastigote which is characteristic of the subgenus Schizotrypanum. Ferritin was used to demonstrate a functional cytostome–cytopharynx complex. Replication of the basal bodies, flagella, kinetoplast, and the nucleus during binary fission is described. Also present were cells which contain multiple sets of organelles and which undergo multiple fission. The ultrastructural features of Trypanosoma myoti resemble those of Trypanosoma cruzi.

Paraliomyces lentiferus: an ultrastructural study of a little-known marine ascomycete

1992 ◽  
Vol 70 (11) ◽  
pp. 2223-2232 ◽  
Author(s):  
S. J. Read ◽  
S.-Y. Hsieh ◽  
E. B. G. Jones ◽  
S. T. Moss ◽  
H. S. Chang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Ultrastructural Study ◽  
Republic Of China ◽  
Mucilaginous Sheath ◽  
Transmission Electron ◽  
Scanning Electron

A collection of Paraliomyces lentiferus from Taiwan, Republic of China, is compared with that of the type description and examined at both scanning and transmission electron microscope levels as part of our review of the taxonomy of the marine Ascomycotina. Particular attention was devoted to the structure of the ascospore appendage. The ascospore wall comprises a mesosporium, an episporium, and a mucilaginous sheath (exosporium?) In addition, there is a single, gelatinous, lateral appendage adjacent to the central septum. The appendage comprises electron-opaque fibrils that in immature ascospores are connected to the ascospore wall via fine electron-opaque strands and larger electron-opaque aggregates of material. The origin of the appendage is discussed. Key words: ascospore, attachment, marine ascomycete, scanning electron microscopy, spore appendage, transmission electron microscopy.

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