An Ultrastructural Study of Chronic Pyelonephritis in Macaca Arctoides

1976 ◽  
Vol 34 ◽  
pp. 310-311
Author(s):  
T.W. Smith ◽  
J.A. Roberts ◽  
B.J. Martin
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Effective Means ◽  
Chronic Pyelonephritis ◽  
Macaca Arctoides ◽  
Left Ureter ◽  
E Coli ◽  
Transmission Electron ◽  
Sizeable Number

Chronic pyelonephritis is one of the most common diseases of the kidney and accounts for a sizeable number of cases of renal insufficiency in man, however its pathogenesis requires further elucidation. Transmission electron microscopy may serve as a uniquely effective means of observing details of the nature of this disease. The present paper describes preliminary results of an ultrastructural study of chronic pyelonephritis in Macaca arctoides (stumptail monkey).The infection was induced in these experiments in a retrograde fashion by means of a unilateral catheterization of the left ureter whereby an innoculum of 10 cc of broth containing approximately 2 billion E. coli per cc and radio-opaque dye were injected under pressure (mimicing vesico-ureteric reflux).

Tumor Diagnosis

1982 ◽  
Vol 40 ◽  
pp. 262-265
Author(s):  
Bruce Mackay
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Differential Diagnosis ◽  
Light Microscopy ◽  
Clinical Examination ◽  
Ultrastructural Study ◽  
Diagnostic Procedures ◽  
Specific Diagnosis ◽  
Transmission Electron ◽  
Microscopic Diagnosis

The broadest application of transmission electron microscopy (EM) in diagnostic medicine is the identification of tumors that cannot be classified by routine light microscopy. EM is useful in the evaluation of approximately 10% of human neoplasms, but the extent of its contribution varies considerably. It may provide a specific diagnosis that can not be reached by other means, but in contrast, the information obtained from ultrastructural study of some 10% of tumors does not significantly add to that available from light microscopy. Most cases fall somewhere between these two extremes: EM may correct a light microscopic diagnosis, or serve to narrow a differential diagnosis by excluding some of the possibilities considered by light microscopy. It is particularly important to correlate the EM findings with data from light microscopy, clinical examination, and other diagnostic procedures.

Spermiogenesis and sperm ultrastructure of Carmyerius endopapillatus (Digenea, Gastrothylacidae), a parasite of Bos taurus in Senegal

2008 ◽  
Vol 53 (1) ◽  
Author(s):  
Momar Seck ◽  
Bernard Marchand ◽  
Cheikh Bâ
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Bos Taurus ◽  
Posterior Extremity ◽  
Sperm Ultrastructure ◽  
Lateral Expansion ◽  
Transmission Electron ◽  
Plasmic Membrane ◽  
Flagellar Rotation

AbstractUltrastructural study of spermiogenesis and of the spermatozoon of Carmyerius endopapillatus has enabled to describe some characteristics of this digenea. The intercentriolar body situated between the two striated roots and the two centrioles, presents a symmetric organization. Both external bands of this intercentriolar body are made up of a row of granules. During spermiogenesis, a flagellar rotation of 90° is described. The old spermatid does not present external ornamentations. The spermatozoon is characterized, in its anterior region, by the presence of a lateral expansion exhibiting one spinelike body. In C. endopapillatus, external ornamentations are localized only at the level where the lateral expansion appears. The posterior extremity of spermatozoon exhibits a nucleus surrounded by a plasmic membrane lacking microtubules, but presenting a small lateral expansion. This is the first species of Gastrothylacidae family studied by transmission electron microscopy.

Dipetalonema viteae: ultrastructural study on the in vitro interaction between rat macrophages and microfilariae in the presence of IgE antibody

Parasitology ◽  
1981 ◽  
Vol 82 (1) ◽  
pp. 55-62 ◽  
Author(s):  
M. A. Ouaissi ◽  
A. Haque ◽  
A. Capron
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Peritoneal Macrophages ◽  
Immune Serum ◽  
Sequence Of Events ◽  
Transmission Electron ◽  
Dipetalonema Viteae ◽  
In Vitro Interaction

SUMMARYThe in vitro interaction between rat peritoneal macrophages and Dipetalonema viteae microfilariae in the presence of amicrofilaraemic rat immune serum was studied by transmission electron microscopy. The probable sequence of events leading to the killing of D. viteae microfilaria by macrophages is as follows. (a) Rat peritoneal macrophages in the presence of amicrofilaraemic rat immune serum adhere to the parasite surface, (b) the macrophages extend their pseudopodia around the parasite, (c) the ‘lysosome-like’ granules discharge their contents on to the parasite surface, (d) the lytic activity of these products begins at the parasite surface and (e) subsequent breaking of the microfilarial cuticle occurs, exposing the parasite intracellular material.

Ultrastructural study of spermatozoa of the paddlefish, Polyodon spathula

Zygote ◽  
2005 ◽  
Vol 13 (3) ◽  
pp. 241-247 ◽  
Author(s):  
Otilia Zarnescu
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Primitive Type ◽  
Polyodon Spathula ◽  
Transmission Electron ◽  
Architectural Features

Paddlefish, Polyodon spathula, spermatozoa were examined by transmission electron microscopy. Their structure has the same characteristic architectural features as sturgeon spermatozoa. Paddlefish spermatozoa are of the primitive type and consist of a rod-shaped head, a midpiece and a long flagellum. The head is about 5.15 mm in length and contains the nucleus and an apical acrosomal complex. Inside the nucleus there are three nuclear channels that begin in the subacrosomal area and have a triple helical arrangement. An nuclear fossa is present centrally, at the posterior end of the nucleus. The midpiece contains a pair of centrioles in a perpendicular arrangement, mitochondria and a narrow cytoplasmic sleeve. The flagellum has a central axoneme with a 9+2 pattern and two lateral projections or fins.

scholarly journals Antimicrobial Properties of a Chitosan Dextran-Based Hydrogel for Surgical Use

2011 ◽  
Vol 56 (1) ◽  
pp. 280-287 ◽  
Author(s):  
Manal A. Aziz ◽  
Jaydee D. Cabral ◽  
Heather J. L. Brooks ◽  
Stephen C. Moratti ◽  
Lyall R. Hanton
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Bacterial Species ◽  
Antimicrobial Properties ◽  
Sinus Surgery ◽  
Peptide Bonds ◽  
Content Type ◽  
E Coli ◽  
Transmission Electron

ABSTRACTA chitosan dextran-based (CD) hydrogel, developed for use in endoscopic sinus surgery, was tested for antimicrobial activityin vitroagainst a range of pathogenic microorganisms. The microdilution technique was used to determine minimum inhibitory, minimum bactericidal, and minimum fungicidal concentrations. In addition, the time-kill efficacy of CD hydrogel was determined for two bacterial species. Scanning and transmission electron microscopy were carried out to elucidate the antimicrobial mechanism of this compound. CD hydrogel was found to be effective againstStaphylococcus aureus,Streptococcus pyogenes,Escherichia coli, andClostridium perfringensat its surgical concentration of 50,000 mg/liter. Minimum bactericidal concentrations ranged from 2,000 to 50,000 mg/liter. Dextran aldehyde (DA) was found to be the antimicrobial component of the CD hydrogel with MBC ranging from 2,000 to 32,000 mg/liter.S. aureusappeared to be killed at a slightly faster rate thanE. coli. Candida albicansandPseudomonas aeruginosawere more resistant to CD hydrogel and DA. Scanning and transmission electron microscopy ofE. coliandS. aureusincubated with CD hydrogel and DA alone revealed morphological damage, disrupted cell walls, and loss of cytosolic contents, compatible with the proposed mode of action involving binding to cell wall proteins and disruption of peptide bonds. Motility and chemotaxis tests showedE. colito be inhibited when incubated with DA. The antibacterial activity of CD hydrogel may make it a useful postsurgical aid at other body sites, especially where there is a risk of Gram-positive infections.

Determination of Bacterial Cell Dry Mass by Transmission Electron Microscopy and Densitometric Image Analysis

1998 ◽  
Vol 64 (2) ◽  
pp. 688-694 ◽  
Author(s):  
M. Loferer-Krößbacher ◽  
J. Klima ◽  
R. Psenner
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Image Analysis ◽  
Bacterial Biomass ◽  
Dry Weight ◽  
Dry Mass ◽  
Bacterial Cells ◽  
E Coli ◽  
Transmission Electron ◽  
Bacterial Assemblages

ABSTRACT We applied transmission electron microscopy and densitometric image analysis to measure the cell volume (V) and dry weight (DW) of single bacterial cells. The system was applied to measure the DW ofEscherichia coli DSM 613 at different growth phases and of natural bacterial assemblages of two lakes, Piburger See and Gossenköllesee. We found a functional allometric relationship between DW (in femtograms) and V (in cubic micrometers) of bacteria (DW = 435 · V 0.86); i.e., smaller bacteria had a higher ratio of DW to V than larger cells. The measured DW of E. coli cells ranged from 83 to 1,172 fg, and V ranged from 0.1 to 3.5 μm3(n = 678). Bacterial cells from Piburger See and Gossenköllesee (n = 465) had DWs from 3 fg (V = 0.003 μm3) to 1,177 fg (V = 3.5 μm3). Between 40 and 50% of the cells had a DW of less than 20 fg. By assuming that carbon comprises 50% of the DW, the ratio of carbon content to Vof individual cells varied from 466 fg of C μm−3 forVs of 0.001 to 0.01 μm3 to 397 fg of C μm−3 (0.01 to 0.1 μm3) and 288 fg of C μm−3 (0.1 to 1 μm3). Exponentially growing and stationary cells of E. coli DSM 613 showed conversion factors of 254 fg of C μm−3 (0.1 to 1 μm3) and 211 fg of C μm−3 (1 to 4 μm3), respectively. Our data suggest that bacterial biomass in aquatic environments is higher and more variable than previously assumed from volume-based measurements.

An ultrastructural study of the culture forms of Trypanosoma (Schizotrypanum) myoti

1983 ◽  
Vol 61 (12) ◽  
pp. 2807-2815 ◽  
Author(s):  
W. Brockley Paterson ◽  
Patrick T. K. Woo
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ultrastructural Study ◽  
Myotis Lucifugus ◽  
Basal Bodies ◽  
Kinetoplast Dna ◽  
Glutaraldehyde Fixation ◽  
Transmission Electron ◽  
Multiple Fission ◽  
Saline Medium

Trypanosoma myoti from the bat Myotis lucifugus was cultured in a diphasic blood-agar and saline medium and examined using transmission electron microscopy. Glutaraldehyde fixation revealed a pattern of kinetoplast DNA condensation in the epimastigote which is characteristic of the subgenus Schizotrypanum. Ferritin was used to demonstrate a functional cytostome–cytopharynx complex. Replication of the basal bodies, flagella, kinetoplast, and the nucleus during binary fission is described. Also present were cells which contain multiple sets of organelles and which undergo multiple fission. The ultrastructural features of Trypanosoma myoti resemble those of Trypanosoma cruzi.

Screening of Condensed Tannins from Canadian Prairie Forages for Anti–Escherichia coli O157:H7 with an Emphasis on Purple Prairie Clover (Dalea purpurea Vent)†

2013 ◽  
Vol 76 (4) ◽  
pp. 560-567 ◽  
Author(s):  
Y. WANG ◽  
L. JIN ◽  
K. H. OMINSKI ◽  
M. HE ◽  
Z. XU ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Transmission Electron Microscopy ◽  
Condensed Tannins ◽  
Brown Seaweed ◽  
Coli Strain ◽  
Escherichia Coli O157 ◽  
Canadian Prairie ◽  
E Coli ◽  
Transmission Electron

Tannins from forages grown (n = 10) on the Canadian prairie, as well as from Quebracho, Rhus semialata, and brown seaweed (Ascophyllum nodosum), were screened for anti–Escherichia coli O157:H7 activity against E. coli O157:H7 strain 3081 at a concentration of 400 μg/ml for each tannin type, except for brown seaweed, which was at 50 μg/ml. Growth of the bacteria was assessed by measuring the optical density at 600 nm over 24 h. Tannin from seaweed at a concentration of 50 μg/ml inhibited growth of strain 3081. Among the terrestrial forages, only condensed tannins (CT) from purple prairie clover (Dalea purpurea Vent; PPC) increased (P < 0.05) the lag time and reduced (P < 0.05) the growth rate of E. coli O157:H7. The anti–E. coli O157:H7 activity of PPC CT was further assessed by culturing E. coli strain ATCC 25922 and eight strains of E. coli O157:H7 with PPC CT at 0, 25, 50, 100, or 200 μg/ml. Selected strains were enumerated after 0, 6, and 24 h of incubation, and fatty acid composition was determined after 24 h of incubation. E. coli strain 25922 was cultured with 0, 50, or 200 μg of CT per ml and harvested during the exponential growth phase for examination by transmission electron microscopy. Increasing CT concentration linearly increased (P < 0.001) the lag times of seven strains and linearly reduced (P < 0.001) the growth rates of eight E. coli O157:H7 strains. Proportions of unsaturated fatty acids in the total fatty acids were decreased (P < 0.01) by CT at 50 μg/ml. Transmission electron microscopy showed that CT disrupted the outer membrane structure. Anti–E. coli O157:H7 activity of PPC CT at levels of up to 200 μg/ml was bacteriostatic rather than bactericidal, and the mechanism of anti–E. coli activity may involve alteration in the fatty acid composition and disruption of the outer membrane of the cell.

Paraliomyces lentiferus: an ultrastructural study of a little-known marine ascomycete

1992 ◽  
Vol 70 (11) ◽  
pp. 2223-2232 ◽  
Author(s):  
S. J. Read ◽  
S.-Y. Hsieh ◽  
E. B. G. Jones ◽  
S. T. Moss ◽  
H. S. Chang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Ultrastructural Study ◽  
Republic Of China ◽  
Mucilaginous Sheath ◽  
Transmission Electron ◽  
Scanning Electron

A collection of Paraliomyces lentiferus from Taiwan, Republic of China, is compared with that of the type description and examined at both scanning and transmission electron microscope levels as part of our review of the taxonomy of the marine Ascomycotina. Particular attention was devoted to the structure of the ascospore appendage. The ascospore wall comprises a mesosporium, an episporium, and a mucilaginous sheath (exosporium?) In addition, there is a single, gelatinous, lateral appendage adjacent to the central septum. The appendage comprises electron-opaque fibrils that in immature ascospores are connected to the ascospore wall via fine electron-opaque strands and larger electron-opaque aggregates of material. The origin of the appendage is discussed. Key words: ascospore, attachment, marine ascomycete, scanning electron microscopy, spore appendage, transmission electron microscopy.

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