scholarly journals Assessment of Immunotoxicity by Multiparameter Flow Cytometry

1997 ◽  
Vol 38 (1) ◽  
pp. 38-54
Author(s):  
Scott W. Burchiel ◽  
Nancy L. Kerkvliet ◽  
G. Frank Gerberick ◽  
David A. Lawrence ◽  
Gregory S. Ladics
Keyword(s):  
Flow Cytometry ◽  
Multiparameter Flow Cytometry

T2050 Immunophenotypic Characterization of the Cellular Infiltrate During Murine Experimental Colitis Using Multiparameter Flow Cytometry

2010 ◽  
Vol 138 (5) ◽  
pp. S-621
Author(s):  
Kimberly A. Zins ◽  
Tamas Ordog ◽  
Michael R. Bardsley ◽  
Gianrico Farrugia ◽  
Joseph H. Szurszewski ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Experimental Colitis ◽  
Multiparameter Flow Cytometry ◽  
Cellular Infiltrate

Immunophenotypic Study of Basophils by Multiparameter Flow Cytometry

2008 ◽  
Vol 132 (5) ◽  
pp. 813-819
Author(s):  
Xiaohong Han ◽  
Jeffrey L. Jorgensen ◽  
Archana Brahmandam ◽  
Ellen Schlette ◽  
Yang O. Huh ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Chronic Myelogenous Leukemia ◽  
Peripheral Blood ◽  
Myelogenous Leukemia ◽  
Multiparameter Flow Cytometry ◽  
Color Flow ◽  
Aberrant Expression ◽  
Flow Cytometric ◽  
Hla Dr

Abstract Context.—The immunophenotypic profile of basophils is not yet fully established, and the immunophenotypic changes in chronic myelogenous leukemia are not fully characterized. Objective.—To establish a comprehensive immunophenotypic spectrum of normal basophils and to assess the range of immunophenotypic aberrations of basophils in chronic myelogenous leukemia. Design.—Using 4-color flow cytometry, we compared the immunophenotypic profile of basophils in peripheral blood or bone marrow samples from 20 patients with no evidence of neoplasia to basophils from 15 patients with chronic myelogenous leukemia. Results.—Basophils in control cases were all positive for CD9, CD13, CD22, CD25 (dim), CD33, CD36, CD38 (bright), CD45 (dimmer than lymphocytes and brighter than myeloblasts), and CD123 (bright), and were negative for CD19, CD34, CD64, CD117, and HLA-DR. Basophils in all chronic myelogenous leukemia patients possessed 1 to 5 immunophenotypic aberrancies. The most common aberrancies were underexpression of CD38, followed by aberrant expression of CD64 and underexpression of CD123. CD34 and CD117 were present in cases with basophilic precursors. Myeloblasts showed a distinct immunophenotypic profile, as they typically expressed CD34 and CD117, showed dimmer expression (compared with basophils) of CD38, CD45, and CD123, and lacked expression of CD22. Conclusions.—Flow cytometric immunophenotyping can identify immunophenotypic aberrations of basophils in chronic myelogenous leukemia, and discriminate basophils from myeloblasts.

scholarly journals Combination of CD157 and FLAER to Detect Peripheral Blood Eosinophils by Multiparameter Flow Cytometry

2015 ◽  
Vol 55 (2) ◽  
pp. 55-60 ◽  
Author(s):  
Giovanni Carulli ◽  
Alessandra Marini ◽  
Paola Sammuri ◽  
Cristiana Domenichini ◽  
Virginia Ottaviano ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Peripheral Blood ◽  
Multiparameter Flow Cytometry ◽  
Blood Eosinophils

scholarly journals Identification of nuclei from apoptotic, necrotic, and viable lymphoid cells by using multiparameter flow cytometry

Cytometry ◽  
1999 ◽  
Vol 35 (2) ◽  
pp. 145-153 ◽  
Author(s):  
Claudia Matteucci ◽  
Sandro Grelli ◽  
Enrico De Smaele ◽  
Carla Fontana ◽  
Antonio Mastino
Keyword(s):  
Flow Cytometry ◽  
Lymphoid Cells ◽  
Multiparameter Flow Cytometry

scholarly journals Characterization of Acanthamoeba–microsphere association by multiparameter flow cytometry and confocal microscopy

2006 ◽  
Vol 69A (4) ◽  
pp. 266-272 ◽  
Author(s):  
Edward A. G. Elloway ◽  
Roger A. Bird ◽  
Christopher J. Hewitt ◽  
Steven L. Kelly ◽  
Stephen N. Smith
Keyword(s):  
Flow Cytometry ◽  
Confocal Microscopy ◽  
Multiparameter Flow Cytometry

Fetal cells in the maternal circulation: isolation by multiparameter flow cytometry and confirmation by polymerase chain reaction

1991 ◽  
Vol 6 (10) ◽  
pp. 1466-1469 ◽  
Author(s):  
Stephen Wachtel ◽  
Sherman Elias ◽  
James Price ◽  
Gwendolyn Wachtel ◽  
Owen Phillips ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Polymerase Chain Reaction ◽  
Multiparameter Flow Cytometry ◽  
Chain Reaction ◽  
Maternal Circulation ◽  
Fetal Cells ◽  
Polymerase Chain

scholarly journals Multiparameter Flow Cytometry Analysis of the Human Spleen Applied to Studies of Plasma-Derived EVs From Plasmodium vivax Patients

2021 ◽  
Vol 11 ◽  
Author(s):  
Melisa Gualdrón-López ◽  
Míriam Díaz-Varela ◽  
Haruka Toda ◽  
Iris Aparici-Herraiz ◽  
Laura Pedró-Cos ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Immune Responses ◽  
Plasmodium Vivax ◽  
Size Exclusion ◽  
Multiparameter Flow Cytometry ◽  
Spleen Cells ◽  
Human Spleen ◽  
Hematopoietic Stem ◽  
Ethical Implications ◽  
Cellular Analysis

The spleen is a secondary lymphoid organ with multiple functions including the removal of senescent red blood cells and the coordination of immune responses against blood-borne pathogens, such as malaria parasites. Despite the major role of the spleen, the study of its function in humans is limited by ethical implications to access human tissues. Here, we employed multiparameter flow cytometry combined with cell purification techniques to determine human spleen cell populations from transplantation donors. Spleen immuno-phenotyping showed that CD45+ cells included B (30%), CD4+ T (16%), CD8+ T (10%), NK (6%) and NKT (2%) lymphocytes. Myeloid cells comprised neutrophils (16%), monocytes (2%) and DCs (0.3%). Erythrocytes represented 70%, reticulocytes 0.7% and hematopoietic stem cells 0.02%. Extracellular vesicles (EVs) are membrane-bound nanoparticles involved in intercellular communication and secreted by almost all cell types. EVs play several roles in malaria that range from modulation of immune responses to vascular alterations. To investigate interactions of plasma-derived EVs from Plasmodium vivax infected patients (PvEVs) with human spleen cells, we used size-exclusion chromatography (SEC) to separate EVs from the bulk of soluble plasma proteins and stained isolated EVs with fluorescent lipophilic dyes. The integrated cellular analysis of the human spleen and the methodology employed here allowed in vitro interaction studies of human spleen cells and EVs that showed an increased proportion of T cells (CD4+ 3 fold and CD8+ 4 fold), monocytes (1.51 fold), B cells (2.3 fold) and erythrocytes (3 fold) interacting with PvEVs as compared to plasma-derived EVs from healthy volunteers (hEVs). Future functional studies of these interactions can contribute to unveil pathophysiological processes involving the spleen in vivax malaria.

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