AACCI Approved Methods Technical Committee Report: A New AACCI Approved Method for the Determination of the Total Carotenoid Content of Cereal Whole Grain and Refined Flours

The influence of certain nitrogen compounds - components of glucose-peptone medium (GPM) on the accumulation of carotenoids by some strains was investigated by surface cultivating basidiomycetes. The total carotenoid content was set in acetone extracts of mycological material spectrophotometrically and calculated using the Vetshteyn formula. As the nitrogen-containing components used GPM with 9 compounds, such as peptone, DL-valine, L-asparagine, DL-serine, DL-tyrosine, L-proline, L-alanine, urea, NaNO3. The effect on the accumulation of specific compounds both in the mycelium and in the culture fluid of carotenoids by culturing certain strains of Basidiomycetes was identified. Adding to standard glucose-peptone medium peptone at 5 g/l causes an increase of carotenoid accumulation by strain L. sulphureus Ls-08, and in a concentration of 4 g/l by strains of F. hepatica Fh-18 and F. fomentarius Ff-1201. In order to increase the accumulation of carotenoids in the mycelium we suggested to make a standard glucose-peptone medium with proline or valine for cultivating of L. sulphureus Ls- 08 strain; alanine for F. fomentarius Ff-1201 strain; proline, asparagine and serine - for strain Fh-18 of F. hepatica. The results can be implemented in further optimization of the composition of the nutrient medium for culturing strains of Basidiomycetes wich producing carotenoids. Keywords: nitrogen-containing substances, Basidiomycetes, mycelium, culture filtrate, carotenoids

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Plant Carotenoids as Pigment Sources in Laying Hen Diets: Effect on Yolk Color, Carotenoid Content, Oxidative Stability and Sensory Properties of Eggs

Foods ◽

10.3390/foods10040721 ◽

2021 ◽

Vol 10 (4) ◽

pp. 721

Author(s):

Kristina Kljak ◽

Klaudija Carović-Stanko ◽

Ivica Kos ◽

Zlatko Janječić ◽

Goran Kiš ◽

...

Keyword(s):

Oxidative Stability ◽

Egg Yolk ◽

Sensory Properties ◽

Carotenoid Content ◽

Total Carotenoid ◽

Plant Parts ◽

Total Carotenoid Content ◽

Dietary Content ◽

Sensory Color ◽

Carotenoid Profile

The aim of this study was to evaluate the effect of a supplementation diet for hens consisting of dried basil herb and flowers of calendula and dandelion for color, carotenoid content, iron-induced oxidative stability, and sensory properties of egg yolk compared with commercial pigment (control) and marigold flower. The plant parts were supplemented in diets at two levels: 1% and 3%. In response to dietary content, yolks from all diets differed in carotenoid profile (p < 0.001). The 3% supplementation level resulted in a similar total carotenoid content as the control (21.25 vs. 21.79 μg/g), but by 3-fold lower compared to the 3% marigold (66.95 μg/g). The tested plants did not achieve yolk color fan values as the control (13.47) or 3% marigold (11.47), and among them, calendula had the highest values (9.73). Despite the low carotenoid content in diets supplemented with basil herb, iron-induced malondialdehyde (MDA) concentration was low as for marigold (on average 106.83 vs. 92.68 ng/g after 250 min). The treatments differed in sensory color scores for fresh and hard-boiled yolks and flavor while other sensory properties were similar. In conclusion, the supplementation of plants in a hen diet may result in yolks containing carotenoids and other compounds showing a high antioxidant effect.

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Molecular cloning, expression pattern of β-carotene 15,15-dioxygenase gene and association analysis with total carotenoid content in pearl oyster Pinctada fucata martensii

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology ◽

10.1016/j.cbpb.2018.11.006 ◽

2019 ◽

Vol 229 ◽

pp. 34-41 ◽

Cited By ~ 2

Author(s):

Chao Lei ◽

Junhui Li ◽

Zhe Zheng ◽

Xiaodong Du ◽

Yuewen Deng

Keyword(s):

Molecular Cloning ◽

Expression Pattern ◽

Association Analysis ◽

Pearl Oyster ◽

Pinctada Fucata ◽

Carotenoid Content ◽

Total Carotenoid ◽

Total Carotenoid Content ◽

Dioxygenase Gene ◽

Β Carotene

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Diet affects body color and energy metabolism in the Baikal endemic amphipod Eulimnogammarus cyaneus maintained in laboratory conditions

Biological Communications ◽

10.21638/spbu03.2021.306 ◽

2021 ◽

Vol 66 (3) ◽

Author(s):

Alexandra Saranchina ◽

Polina Drozdova ◽

Andrei Mutin ◽

Maxim Timofeyev

Keyword(s):

Glycogen Content ◽

Diet Group ◽

Environmental Research ◽

Carotenoid Content ◽

Total Carotenoid ◽

Body Color ◽

Commercial Diet ◽

Total Carotenoid Content ◽

Laboratory Bioassays

Proper diet is critical for laboratory-reared animals, as it may affect not only their welfare, but also experimental results. Amphipods (Crustacea: Amphipoda) play important roles in ecosystems and are often used in environmental research. Endemic amphipods from the ancient Lake Baikal are promising for laboratory bioassays; however, there are currently no laboratory cultures. In this work, we determine how different diets affect the color and metabolism of a laboratory-reared Baikal amphipod, Eulimnogammarus cyaneus. We found that in freshly collected blue-colored animals, body color correlated with total carotenoid content. Total carotenoid levels did not differ after long-term (two months) feeding with a close to natural carotenoid-enriched, or even a carotenoid-depleted diet. Nevertheless, antennae color was closer to red in the natural-like diet group. It is likely that the carotenoids from the commercial diet are not properly metabolized in E. cyaneus. The animals fed commercial diets had a higher glycogen content, which may signify a higher metabolic rate. Overall, we show that a carotenoid-enriched diet optimized for decapods is not optimal for amphipods, likely due to different carotenoid compositions, and the diet for long-term rearing of E. cyaneus and other Baikal amphipods requires supplementation.

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