scholarly journals Genome Sequence Resources for the Wheat Stripe Rust Pathogen (Puccinia striiformis f. sp. tritici) and the Barley Stripe Rust Pathogen (Puccinia striiformis f. sp. hordei)

2018 ◽  
Vol 31 (11) ◽  
pp. 1117-1120 ◽  
Author(s):  
Chongjing Xia ◽  
Meinan Wang ◽  
Chuntao Yin ◽  
Omar E. Cornejo ◽  
Scot H. Hulbert ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Molecular Mechanisms ◽  
Puccinia Striiformis ◽  
Yellow Rust ◽  
Rapid Evolution ◽  
Host Adaptation ◽  
Rust Pathogen ◽  
Structural Differences ◽  
Barley Stripe Rust ◽  
Genomic Basis

Puccinia striiformis f. sp. tritici causes devastating stripe (yellow) rust on wheat and P. striiformis f. sp. hordei causes stripe rust on barley. Several P. striiformis f. sp. tritici genomes are available, but no P. striiformis f. sp. hordei genome is available. More genomes of P. striiformis f. sp. tritici and P. striiformis f. sp. hordei are needed to understand the genome evolution and molecular mechanisms of their pathogenicity. We sequenced P. striiformis f. sp. tritici isolate 93-210 and P. striiformis f. sp. hordei isolate 93TX-2, using PacBio and Illumina technologies and RNA sequencing. Their genomic sequences were assembled to contigs with high continuity and showed significant structural differences. The circular mitochondria genomes of both were complete. These genomes provide high-quality resources for deciphering the genomic basis of rapid evolution and host adaptation, identifying genes for avirulence and other important traits, and studying host-pathogen interactions.

scholarly journals Whole genome resequencing and comparative genome analysis of three Puccinia striiformis f. sp. tritici pathotypes prevalent in India

2021 ◽  
Author(s):  
Inderjit Singh Yadav ◽  
S. C. Bhardwaj ◽  
Jaspal Kaur ◽  
Deepak Singla ◽  
Satinder Kaur ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Yellow Rust ◽  
Strong Association ◽  
Rapid Evolution ◽  
Whole Genome ◽  
Rust Disease ◽  
The North ◽  
Whole Genome Resequencing

Stripe rust disease of wheat, caused by Puccinia striiformis f. sp. tritici, ( Pst ) is one of the most serious diseases of wheat worldwide. In India, virulent stripe rust races have been constantly evolving in the North-Western Plains Zone leading to the failure of some of the most widely grown resistant varieties in the region. With the goal of studying the recent evolution of virulent races in this region, we conducted whole-genome sequencing of three prevalent Indian Pst pathotypes Pst46S119, Pst78S84 and Pst110S119. We assembled 58.62, 58.33 and 55.78 Mb of Pst110S119, Pst46S119 and Pst78S84 genome, respectively. Pathotypes were found to be highly heterozygous. Comparative phylogenetic analysis indicated the recent evolution of pathotypes Pst110S119 and Pst78S84 from Pst46S119. Pathogenicity-related genes classes (CAZyme, proteases, effectors, and secretome proteins) were identified and found to be under positive selection. Higher rate of gene family expansion was also observed in the three pathotypes. A strong association between the effector genes and transposable elements may be the source of the rapid evolution of these strains. Phylogenetic analysis differentiated the Indian races in this study from other known US, European, African and Asian races. Diagnostic markers developed for the identification of different Pst pathotypes will help tracking of yellow rust at farmers’ field and strategizing resistance gene deployment.

scholarly journals Virulence and Molecular Characterization of Experimental Isolates of the Stripe Rust Pathogen (Puccinia striiformis) Indicate Somatic Recombination

2017 ◽  
Vol 107 (3) ◽  
pp. 329-344 ◽  
Author(s):  
Yu Lei ◽  
Meinan Wang ◽  
Anmin Wan ◽  
Chongjing Xia ◽  
Deven R. See ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Somatic Recombination ◽  
Rust Pathogen ◽  
Barley Stripe Rust ◽  
Barley Genotypes ◽  
Simple Sequence

Puccinia striiformis causes stripe rust on wheat, barley, and grasses. Natural population studies have indicated that somatic recombination plays a possible role in P. striiformis variation. To determine whether somatic recombination can occur, susceptible wheat or barley plants were inoculated with mixed urediniospores of paired isolates of P. striiformis. Progeny isolates were selected by passing through a series of inoculations of wheat or barley genotypes. Potential recombinant isolates were compared with the parental isolates on the set of 18 wheat or 12 barley genotypes that are used to differentiate races of P. striiformis f. sp. tritici (the wheat stripe rust pathogen) and P. striiformis f. sp. hordei (the barley stripe rust pathogen), respectively, for virulence changes. They were also tested with 51 simple-sequence repeat and 90 single-nucleotide polymorphism markers for genotype changes. From 68 possible recombinant isolates obtained from nine combinations of isolates based on virulence tests, 66 were proven to be recombinant isolates by molecular markers. Various types of recombinants were determined, including lost virulence from both virulent parental isolates, gained virulence from both avirulent isolates, combined virulences from both parents, and inherited virulence from one parent and avirulence from another. Marker data indicate that most of the recombinants were produced through chromosome reassortment and crossover after the hybridization of two parental isolates. The results demonstrate that somatic recombination is a mechanism by which new variants can be generated in P. striiformis.

scholarly journals Potential Infection Risks of the Wheat Stripe Rust and Stem Rust Pathogens on Barberry in Asia and Southeastern Europe

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 957
Author(s):  
Parimal Sinha ◽  
Xianming Chen
Keyword(s):  
Stripe Rust ◽  
Stem Rust ◽  
Puccinia Striiformis ◽  
Infection Risk ◽  
Southeastern Europe ◽  
Alternate Host ◽  
Modeling Framework ◽  
Risk Levels ◽  
Rust Pathogen ◽  
Very High

Barberry (Berberis spp.) is an alternate host for both the stripe rust pathogen, Puccinia striiformis f. sp. tritici (Pst), and the stem rust pathogen, P. graminis f. sp. tritici (Pgt), infecting wheat. Infection risk was assessed to determine whether barberry could be infected by either of the pathogens in Asia and Southeastern Europe, known for recurring epidemics on wheat and the presence of barberry habitats. For assessing infection risk, mechanistic infection models were used to calculate infection indices for both pathogens on barberry following a modeling framework. In East Asia, Bhutan, China, and Nepal were found to have low risks of barberry infection by Pst but high risks by Pgt. In Central Asia, Azerbaijan, Iran, Kazakhstan, southern Russia, and Uzbekistan were identified to have low to high risks of barberry infection for both Pst and Pgt. In Northwest Asia, risk levels of both pathogens in Turkey and the Republic of Georgia were determined to be high to very high. In Southwest Asia, no or low risk was found. In Southeastern Europe, similar high or very high risks for both pathogens were noted for all countries. The potential risks of barberry infection by Pst and/or Pgt should provide guidelines for monitoring barberry infections and could be valuable for developing rust management programs in these regions. The framework used in this study may be useful to predict rust infection risk in other regions.

Distribution of Puccinia striiformis f. sp. tritici races and virulence in wheat growing regions of Kenya from 1970 TO 2014

Plant Disease ◽  
2021 ◽  
Author(s):  
Mercy Wamalwa ◽  
Ruth Wanyera ◽  
Julian Rodriguez-Algaba ◽  
Lesley Boyd ◽  
James Owuoche ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Fungal Pathogen ◽  
Puccinia Striiformis ◽  
Yellow Rust ◽  
Stripe Rust Resistance ◽  
Scar Markers ◽  
Seedling Resistance ◽  
Sequence Characterized Amplified Region ◽  
Triticum Spp ◽  
Virulence Diversity

Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a major threat to wheat (Triticum spp.) production worldwide. The objective of this study was to determine the virulence of Pst races prevalent in the main wheat growing regions of Kenya, which includes Mt. Kenya, Eastern Kenya, and the Rift Valley (Central, Southern, and Northern Rift). Fifty Pst isolates collected from 1970 to 1992 and from 2009 to 2014 were virulence phenotyped using stripe rust differential sets, and 45 isolates were genotyped with sequence characterized amplified region (SCAR) markers to differentiate among the isolates and identify aggressive strains PstS1 and PstS2. Virulence corresponding to stripe rust resistance genes Yr1, Yr2, Yr3, Yr6, Yr7, Yr8, Yr9, Yr17, Yr25, Yr27 and the seedling resistance in genotype Avocet S were detected. Ten races were detected in the Pst samples obtained from 1970 to 1992, and three additional races were detected from 2009 to 2014, with a single race being detected in both periods. The SCAR markers detected both Pst1 and Pst2 strains in the collection. Increasing Pst virulence was found in the Kenyan Pst population, and that diverse Pst race groups dominated different wheat growing regions. Moreover, recent Pst races in east Africa indicated possible migration of some race groups into Kenya from other regions. This study is important in understanding Pst evolution and virulence diversity and useful in breeding wheat cultivars with effective resistance to stripe rust. Keywords: pathogenicity, Puccinia f. sp. tritici stripe (yellow) rust, Triticum aestivum

Microsatellite Genotyping of the Wheat Yellow Rust Pathogen Puccinia striiformis

2017 ◽  
pp. 59-70 ◽  
Author(s):  
Sajid Ali ◽  
Muhammad R. Khan ◽  
Angelique Gautier ◽  
Zahoor A. Swati ◽  
Stephanie Walter
Keyword(s):  
Puccinia Striiformis ◽  
Yellow Rust ◽  
Microsatellite Genotyping ◽  
Rust Pathogen

Overwintering potential of the stripe rust pathogen (Puccinia striiformis) in central Alberta

2013 ◽  
Vol 35 (3) ◽  
pp. 304-314 ◽  
Author(s):  
K. Kumar ◽  
M. D. Holtz ◽  
K. Xi ◽  
T. K. Turkington
Keyword(s):  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Rust Pathogen

scholarly journals The barley immune receptor Mla recognizes multiple pathogens and contributes to host range dynamics

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jan Bettgenhaeuser ◽  
Inmaculada Hernández-Pinzón ◽  
Andrew M. Dawson ◽  
Matthew Gardiner ◽  
Phon Green ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Stripe Rust ◽  
Resistance Genes ◽  
Host Species ◽  
Plant Pathogens ◽  
Puccinia Striiformis ◽  
Species Specificity ◽  
Wheat Stripe Rust ◽  
Barley Powdery Mildew ◽  
Barley Stripe Rust

AbstractCrop losses caused by plant pathogens are a primary threat to stable food production. Stripe rust (Puccinia striiformis) is a fungal pathogen of cereal crops that causes significant, persistent yield loss. Stripe rust exhibits host species specificity, with lineages that have adapted to infect wheat and barley. While wheat stripe rust and barley stripe rust are commonly restricted to their corresponding hosts, the genes underlying this host specificity remain unknown. Here, we show that three resistance genes, Rps6, Rps7, and Rps8, contribute to immunity in barley to wheat stripe rust. Rps7 cosegregates with barley powdery mildew resistance at the Mla locus. Using transgenic complementation of different Mla alleles, we confirm allele-specific recognition of wheat stripe rust by Mla. Our results show that major resistance genes contribute to the host species specificity of wheat stripe rust on barley and that a shared genetic architecture underlies resistance to the adapted pathogen barley powdery mildew and non-adapted pathogen wheat stripe rust.

scholarly journals Management of stripe rust of barley (Hordeum vulgare L.) using fungicides

2015 ◽  
Vol 7 (1) ◽  
pp. 170-174
Author(s):  
Rakesh Devlash ◽  
Naval Kishore ◽  
Guru Dev Singh
Keyword(s):  
Hordeum Vulgare ◽  
Disease Severity ◽  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Field Conditions ◽  
Hordeum Vulgare L ◽  
Grain Yields ◽  
Untreated Check ◽  
Rust Severity ◽  
Barley Stripe Rust

Under field conditions, various fungicide molecules were validated for their effectiveness on barley (Hordeum vulgare L.) stripe rust Puccinia striiformis f. sp. consecutively for three years under artificial field epiphytotic conditions. Seven fungicides viz., propiconazole 25%EC (tilt @ 0.1%), tebuconazole 25.9% m/m EC (folicur @ 0.1%), triademefon 25%WP (bayleton @ 0.1%), propiconazole 25%EC (tilt @ 0.05%), tebuconazole 25.9% m/m EC (folicur @ 0.05%), triademefon 25%WP (bayleton@ 0.05%), and mancozeb 75%WP (dithane M45 @ 0.2%) with variousconcentrations were tested for their effectiveness in controlling barley stripe rust severity. All fungicide applications resulted in lower disease severity and higher grain yields than untreated check plots. All the fungicides @ 0.1% concentrations reduced disease severity ranging from 87.8% to 95.6% except Mancozeb @ 0.2% (34.4%). Significant higher yield was obtained with Propiconazole @ 0.1% (26.7 q/ha) followed by Tebuconazole @ 0.1% (25.2 q/ha) and Triademefon @ 0.1% (24.5 q/ha). The present study revealed propiconazole as the most effective fungicide for the control of stripe rust of barley under epiphytotic conditions.

scholarly journals Inheritance and Molecular Mapping of Barley Genes Conferring Resistance to Wheat Stripe Rust

2005 ◽  
Vol 95 (8) ◽  
pp. 884-889 ◽  
Author(s):  
Vihanga Pahalawatta ◽  
Xianming Chen
Keyword(s):  
Resistance Gene ◽  
Microsatellite Marker ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Puccinia Striiformis ◽  
Infection Type ◽  
Dominant Gene ◽  
Wheat Stripe Rust ◽  
Rust Pathogen ◽  
Type Data

Most barley cultivars are resistant to stripe rust of wheat that is caused by Puccinia striiformis f. sp. tritici. The barley cv. Steptoe is susceptible to all identified races of P. striiformis f. sp. hordei (PSH), the barley stripe rust pathogen, but is resistant to most P. striiformis f. sp. tritici races. To determine inheritance of the Steptoe resistance to P. striiformis f. sp. tritici, a cross was made between Steptoe and Russell, a barley cultivar susceptible to some P. striiformis f. sp. tritici races and all tested P. striiformis f. sp. hordei races. Seedlings of parents and F1, BC1, F2, and F3 progeny from the barley cross were tested with P. striiformis f. sp. tritici races PST-41 and PST-45 under controlled greenhouse conditions. Genetic analyses of infection type data showed that Steptoe had one dominant gene and one recessive gene (provisionally designated as RpstS1 and rpstS2, respectively) for resistance to races PST-41 and PST-45. Genomic DNA was extracted from the parents and 150 F2 plants that were tested for rust reaction and grown for seed of F3 lines. The infection type data and polymorphic markers identified using the resistance gene analog polymorphism (RGAP) technique were analyzed with the Mapmaker computer program to map the resistance genes. The dominant resistance gene in Steptoe for resistance to P. striiformis f. sp. tritici races was mapped on barley chromosome 4H using a linked microsatellite marker, HVM68. A linkage group for the dominant gene was constructed with 12 RGAP markers and the microsatellite marker. The results show that resistance in barley to the wheat stripe rust pathogen is qualitatively inherited. These genes might provide useful resistance against wheat stripe rust when introgressed into wheat from barley.

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