Virulence and Molecular Characterization of Experimental Isolates of the Stripe Rust Pathogen (Puccinia striiformis) Indicate Somatic Recombination

Puccinia striiformis causes stripe rust on wheat, barley, and grasses. Natural population studies have indicated that somatic recombination plays a possible role in P. striiformis variation. To determine whether somatic recombination can occur, susceptible wheat or barley plants were inoculated with mixed urediniospores of paired isolates of P. striiformis. Progeny isolates were selected by passing through a series of inoculations of wheat or barley genotypes. Potential recombinant isolates were compared with the parental isolates on the set of 18 wheat or 12 barley genotypes that are used to differentiate races of P. striiformis f. sp. tritici (the wheat stripe rust pathogen) and P. striiformis f. sp. hordei (the barley stripe rust pathogen), respectively, for virulence changes. They were also tested with 51 simple-sequence repeat and 90 single-nucleotide polymorphism markers for genotype changes. From 68 possible recombinant isolates obtained from nine combinations of isolates based on virulence tests, 66 were proven to be recombinant isolates by molecular markers. Various types of recombinants were determined, including lost virulence from both virulent parental isolates, gained virulence from both avirulent isolates, combined virulences from both parents, and inherited virulence from one parent and avirulence from another. Marker data indicate that most of the recombinants were produced through chromosome reassortment and crossover after the hybridization of two parental isolates. The results demonstrate that somatic recombination is a mechanism by which new variants can be generated in P. striiformis.

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Cloning and characterization of a wheat β-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. tritici

Molecular Biology Reports ◽

10.1007/s11033-009-9823-9 ◽

2009 ◽

Vol 37 (2) ◽

pp. 1045-1052 ◽

Cited By ~ 45

Author(s):

Bo Liu ◽

Xiaodan Xue ◽

Suping Cui ◽

Xiaoyu Zhang ◽

Qingmei Han ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Rust Pathogen

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Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici)

BMC Genomics ◽

10.1186/1471-2164-8-145 ◽

2007 ◽

Vol 8 (1) ◽

pp. 145 ◽

Cited By ~ 39

Author(s):

Peng Ling ◽

Meinan Wang ◽

Xianming Chen ◽

Kimberly Campbell

Keyword(s):

Cdna Library ◽

Stripe Rust ◽

Puccinia Striiformis ◽

Full Length ◽

Wheat Stripe Rust ◽

Full Length Cdna ◽

Rust Pathogen

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Simple Sequence Repeat (SSR) and Single Nucleotide Polymorphism (SNP) Markers for Genetic Characterization of North Sulawesi Local Rice Varieties, Super Win and Burungan

Pakistan Journal of Biological Sciences ◽

10.3923/pjbs.2017.447.456 ◽

2017 ◽

Vol 20 (9) ◽

pp. 447-456

Author(s):

Marjam Magdalena Toding ◽

Trina Ekawati Tallei ◽

Dwinita Wikan Utami

Keyword(s):

Simple Sequence Repeat ◽

Genetic Characterization ◽

Snp Markers ◽

Sequence Repeat ◽

Nucleotide Polymorphism ◽

Rice Varieties ◽

Single Nucleotide ◽

North Sulawesi ◽

Simple Sequence

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Genomic insights into host adaptation between the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici) and the barley stripe rust pathogen (Puccinia striiformis f. sp. hordei)

BMC Genomics ◽

10.1186/s12864-018-5041-y ◽

2018 ◽

Vol 19 (1) ◽

Cited By ~ 18

Author(s):

Chongjing Xia ◽

Meinan Wang ◽

Chuntao Yin ◽

Omar E. Cornejo ◽

Scot H. Hulbert ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Host Adaptation ◽

Wheat Stripe Rust ◽

Rust Pathogen ◽

Barley Stripe Rust

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Genome Sequence Resources for the Wheat Stripe Rust Pathogen (Puccinia striiformis f. sp. tritici) and the Barley Stripe Rust Pathogen (Puccinia striiformis f. sp. hordei)

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-04-18-0107-a ◽

2018 ◽

Vol 31 (11) ◽

pp. 1117-1120 ◽

Cited By ~ 7

Author(s):

Chongjing Xia ◽

Meinan Wang ◽

Chuntao Yin ◽

Omar E. Cornejo ◽

Scot H. Hulbert ◽

...

Keyword(s):

Stripe Rust ◽

Molecular Mechanisms ◽

Puccinia Striiformis ◽

Yellow Rust ◽

Rapid Evolution ◽

Host Adaptation ◽

Rust Pathogen ◽

Structural Differences ◽

Barley Stripe Rust ◽

Genomic Basis

Puccinia striiformis f. sp. tritici causes devastating stripe (yellow) rust on wheat and P. striiformis f. sp. hordei causes stripe rust on barley. Several P. striiformis f. sp. tritici genomes are available, but no P. striiformis f. sp. hordei genome is available. More genomes of P. striiformis f. sp. tritici and P. striiformis f. sp. hordei are needed to understand the genome evolution and molecular mechanisms of their pathogenicity. We sequenced P. striiformis f. sp. tritici isolate 93-210 and P. striiformis f. sp. hordei isolate 93TX-2, using PacBio and Illumina technologies and RNA sequencing. Their genomic sequences were assembled to contigs with high continuity and showed significant structural differences. The circular mitochondria genomes of both were complete. These genomes provide high-quality resources for deciphering the genomic basis of rapid evolution and host adaptation, identifying genes for avirulence and other important traits, and studying host-pathogen interactions.

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Genetic Analysis and Molecular Mapping of Wheat Genes Conferring Resistance to the Wheat Stripe Rust and Barley Stripe Rust Pathogens

Phytopathology ◽

10.1094/phyto-95-0427 ◽

2005 ◽

Vol 95 (4) ◽

pp. 427-432 ◽

Cited By ~ 29

Author(s):

Vihanga Pahalawatta ◽

Xianming Chen

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Molecular Mapping ◽

Puccinia Striiformis ◽

Dominant Gene ◽

Wheat Genotypes ◽

Barley Cultivars ◽

Barley Stripe Rust ◽

Rust Pathogens ◽

Barley Genotypes

Stripe rust is one of the most important diseases of wheat and barley worldwide. On wheat it is caused by Puccinia striiformis f. sp. tritici and on barley by P. striiformis f. sp. hordei Most wheat genotypes are resistant to P. striiformis f. sp. hordei and most barley genotypes are resistant to P. striiformis f. sp. tritici. To determine the genetics of resistance in wheat to P. striiformis f. sp. hordei, crosses were made between wheat genotypes Lemhi (resistant to P. striiformis f. sp. hordei) and PI 478214 (susceptible to P. striiformis f. sp. hordei). The greenhouse seedling test of 150 F2 progeny from the Lemhi × PI 478214 cross, inoculated with race PSH-14 of P. striiformis f. sp. hordei, indicated that Lemhi has a dominant resistance gene. The single dominant gene was confirmed by testing seedlings of the F1, BC1 to the two parents, and 150 F3 lines from the F2 plants with the same race. The tests of the F1, BC1, and F3 progeny with race PSH-48 of P. striiformis f. sp. hordei and PST-21 of P. striiformis f. sp. tritici also showed a dominant gene for resistance to these races. Cosegregation analyses of the F3 data from the tests with the two races of P. striiformis f. sp. hordei and one race of P. striiformis f. sp. tritici suggested that the same gene conferred the resistance to both races of P. striiformis f. sp. hordei, and this gene was different but closely linked to Yr21, a previously reported gene in Lemhi conferring resistance to race PST-21 of P. striiformis f. sp. tritici. A linkage group consisting of 11 resistance gene analog polymorphism (RGAP) markers was established for the genes. The gene was confirmed to be on chromosome 1B by amplification of a set of nullitetrasomic Chinese Spring lines with an RGAP marker linked in repulsion with the resistance allele. The genetic information obtained from this study is useful in understanding interactions between inappropriate hosts and pathogens. The gene identified in Lemhi for resistance to P. striiformis f. sp. hordei should provide resistance to barley stripe rust when introgressed into barley cultivars.

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Faculty Opinions recommendation of Genome-wide single nucleotide polymorphism analysis reveals frequent partial uniparental disomy due to somatic recombination in acute myeloid leukemias.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.12528.470012 ◽

2006 ◽

Author(s):

Andrea Biondi

Keyword(s):

Single Nucleotide Polymorphism ◽

Uniparental Disomy ◽

Polymorphism Analysis ◽

Single Nucleotide Polymorphism Analysis ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Somatic Recombination ◽

Genome Wide ◽

Myeloid Leukemias ◽

Acute Myeloid

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Genetic Mapping by Sequencing More Precisely Detects Loci Responsible for Anaerobic Germination Tolerance in Rice

Plants ◽

10.3390/plants10040705 ◽

2021 ◽

Vol 10 (4) ◽

pp. 705

Author(s):

John Carlos I. Ignacio ◽

Maricris Zaidem ◽

Carlos Casal ◽

Shalabh Dixit ◽

Tobias Kretzschmar ◽

...

Keyword(s):

Genetic Basis ◽

Snp Markers ◽

Nucleotide Polymorphism ◽

Rice Varieties ◽

Single Nucleotide ◽

Genotyping Platform ◽

Anaerobic Germination ◽

Direct Seeded ◽

Mapping By Sequencing ◽

Simple Sequence

Direct seeded rice (DSR) is a mainstay for planting rice in the Americas, and it is rapidly becoming more popular in Asia. It is essential to develop rice varieties that are suitable for this type of production system. ASD1, a landrace from India, possesses several traits desirable for direct-seeded fields, including tolerance to anaerobic germination (AG). To map the genetic basis of its tolerance, we examined a population of 200 F2:3 families derived from a cross between IR64 and ASD1 using the restriction site-associated DNA sequencing (RAD-seq) technology. This genotyping platform enabled the identification of 1921 single nucleotide polymorphism (SNP) markers to construct a high-resolution genetic linkage map with an average interval of 0.9 cM. Two significant quantitative trait loci (QTLs) were detected on chromosomes 7 and 9, qAG7 and qAG9, with LOD scores of 7.1 and 15.0 and R2 values of 15.1 and 29.4, respectively. Here, we obtained more precise locations of the QTLs than traditional simple sequence repeat and low-density SNP genotyping methods and may help further dissect the genetic factors of these QTLs.

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