scholarly journals Genomic insights into host adaptation between the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici) and the barley stripe rust pathogen (Puccinia striiformis f. sp. hordei)

BMC Genomics ◽  
2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Chongjing Xia ◽  
Meinan Wang ◽  
Chuntao Yin ◽  
Omar E. Cornejo ◽  
Scot H. Hulbert ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Host Adaptation ◽  
Wheat Stripe Rust ◽  
Rust Pathogen ◽  
Barley Stripe Rust

scholarly journals Genome Sequence Resources for the Wheat Stripe Rust Pathogen (Puccinia striiformis f. sp. tritici) and the Barley Stripe Rust Pathogen (Puccinia striiformis f. sp. hordei)

2018 ◽  
Vol 31 (11) ◽  
pp. 1117-1120 ◽  
Author(s):  
Chongjing Xia ◽  
Meinan Wang ◽  
Chuntao Yin ◽  
Omar E. Cornejo ◽  
Scot H. Hulbert ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Molecular Mechanisms ◽  
Puccinia Striiformis ◽  
Yellow Rust ◽  
Rapid Evolution ◽  
Host Adaptation ◽  
Rust Pathogen ◽  
Structural Differences ◽  
Barley Stripe Rust ◽  
Genomic Basis

Puccinia striiformis f. sp. tritici causes devastating stripe (yellow) rust on wheat and P. striiformis f. sp. hordei causes stripe rust on barley. Several P. striiformis f. sp. tritici genomes are available, but no P. striiformis f. sp. hordei genome is available. More genomes of P. striiformis f. sp. tritici and P. striiformis f. sp. hordei are needed to understand the genome evolution and molecular mechanisms of their pathogenicity. We sequenced P. striiformis f. sp. tritici isolate 93-210 and P. striiformis f. sp. hordei isolate 93TX-2, using PacBio and Illumina technologies and RNA sequencing. Their genomic sequences were assembled to contigs with high continuity and showed significant structural differences. The circular mitochondria genomes of both were complete. These genomes provide high-quality resources for deciphering the genomic basis of rapid evolution and host adaptation, identifying genes for avirulence and other important traits, and studying host-pathogen interactions.

scholarly journals The barley immune receptor Mla recognizes multiple pathogens and contributes to host range dynamics

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jan Bettgenhaeuser ◽  
Inmaculada Hernández-Pinzón ◽  
Andrew M. Dawson ◽  
Matthew Gardiner ◽  
Phon Green ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Stripe Rust ◽  
Resistance Genes ◽  
Host Species ◽  
Plant Pathogens ◽  
Puccinia Striiformis ◽  
Species Specificity ◽  
Wheat Stripe Rust ◽  
Barley Powdery Mildew ◽  
Barley Stripe Rust

AbstractCrop losses caused by plant pathogens are a primary threat to stable food production. Stripe rust (Puccinia striiformis) is a fungal pathogen of cereal crops that causes significant, persistent yield loss. Stripe rust exhibits host species specificity, with lineages that have adapted to infect wheat and barley. While wheat stripe rust and barley stripe rust are commonly restricted to their corresponding hosts, the genes underlying this host specificity remain unknown. Here, we show that three resistance genes, Rps6, Rps7, and Rps8, contribute to immunity in barley to wheat stripe rust. Rps7 cosegregates with barley powdery mildew resistance at the Mla locus. Using transgenic complementation of different Mla alleles, we confirm allele-specific recognition of wheat stripe rust by Mla. Our results show that major resistance genes contribute to the host species specificity of wheat stripe rust on barley and that a shared genetic architecture underlies resistance to the adapted pathogen barley powdery mildew and non-adapted pathogen wheat stripe rust.

scholarly journals Inheritance and Molecular Mapping of Barley Genes Conferring Resistance to Wheat Stripe Rust

2005 ◽  
Vol 95 (8) ◽  
pp. 884-889 ◽  
Author(s):  
Vihanga Pahalawatta ◽  
Xianming Chen
Keyword(s):  
Resistance Gene ◽  
Microsatellite Marker ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Puccinia Striiformis ◽  
Infection Type ◽  
Dominant Gene ◽  
Wheat Stripe Rust ◽  
Rust Pathogen ◽  
Type Data

Most barley cultivars are resistant to stripe rust of wheat that is caused by Puccinia striiformis f. sp. tritici. The barley cv. Steptoe is susceptible to all identified races of P. striiformis f. sp. hordei (PSH), the barley stripe rust pathogen, but is resistant to most P. striiformis f. sp. tritici races. To determine inheritance of the Steptoe resistance to P. striiformis f. sp. tritici, a cross was made between Steptoe and Russell, a barley cultivar susceptible to some P. striiformis f. sp. tritici races and all tested P. striiformis f. sp. hordei races. Seedlings of parents and F1, BC1, F2, and F3 progeny from the barley cross were tested with P. striiformis f. sp. tritici races PST-41 and PST-45 under controlled greenhouse conditions. Genetic analyses of infection type data showed that Steptoe had one dominant gene and one recessive gene (provisionally designated as RpstS1 and rpstS2, respectively) for resistance to races PST-41 and PST-45. Genomic DNA was extracted from the parents and 150 F2 plants that were tested for rust reaction and grown for seed of F3 lines. The infection type data and polymorphic markers identified using the resistance gene analog polymorphism (RGAP) technique were analyzed with the Mapmaker computer program to map the resistance genes. The dominant resistance gene in Steptoe for resistance to P. striiformis f. sp. tritici races was mapped on barley chromosome 4H using a linked microsatellite marker, HVM68. A linkage group for the dominant gene was constructed with 12 RGAP markers and the microsatellite marker. The results show that resistance in barley to the wheat stripe rust pathogen is qualitatively inherited. These genes might provide useful resistance against wheat stripe rust when introgressed into wheat from barley.

First Report of Stripe Rust caused by Puccinia striiformis f. sp. tritici on Wheat in Florida

2005 ◽  
Vol 6 (1) ◽  
pp. 32 ◽  
Author(s):  
Ann R. Blount ◽  
Shabbir A. Rizvi ◽  
Ronald D. Barnett ◽  
Xianming Chen ◽  
Timothy S. Schubert ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Wheat Stripe Rust ◽  
First Report ◽  
Documented Case ◽  
Rust Pathogen ◽  
Wheat Lines ◽  
Yield Trials

The wheat stripe rust pathogen occured on several experimental wheat lines planted at Quincy, FL in early February 2003. Several experimental lines in the 2003 Advanced Wheat A, the Advanced Wheat B, and the Uniform Southern Wheat Nursery yield trials then showed traces of stripe rust on the leaves of the plants. An unusually cool and wet winter and spring encouraged a scattered outbreak of stripe rust of wheat on susceptible experimental lines of wheat. This report constitutes the first documented case of stripe rust of wheat in Florida. Accepted for publication 22 February 2005. Published 4 March 2005.

scholarly journals Constructing Physical and Genomic Maps forPuccinia striiformisf. sp.tritici, the Wheat Stripe Rust Pathogen, by Comparing Its EST Sequences to the Genomic Sequence ofP. graminisf. sp.tritici, the Wheat Stem Rust Pathogen

2009 ◽  
Vol 2009 ◽  
pp. 1-13 ◽  
Author(s):  
Jinbiao Ma ◽  
Xianming Chen ◽  
Meinan Wang ◽  
Zhensheng Kang
Keyword(s):  
Stripe Rust ◽  
Stem Rust ◽  
Genomic Sequence ◽  
Molecular Mapping ◽  
Puccinia Striiformis ◽  
Rust Fungus ◽  
Wheat Stem Rust ◽  
Wheat Stripe Rust ◽  
Physical Relationship ◽  
Rust Pathogen

The wheat stripe rust fungus,Puccinia striiformisf. sp.tritici(Pst), does not have a known alternate host for sexual reproduction, which makes it impossible to study gene linkages through classic genetic and molecular mapping approaches. In this study, we compared 4,219Pstexpression sequence tags (ESTs) to the genomic sequence ofP. graminisf. sp.tritici(Pgt), the wheat stem rust fungus, using BLAST searches. The percentages of homologous genes varied greatly among differentPstlibraries with 54.51%, 51.21%, and 13.61% for the urediniospore, germinated urediniospore, and haustorial libraries, respectively, with an average of 33.92%. The 1,432Pstgenes with significant homology withPgtsequences were grouped into physical groups corresponding to 237Pgtsupercontigs. The physical relationship was demonstrated by 12 pairs (57%), out of 21 selectedPstgene pairs, through PCR screening of aPstBAC library. The results indicate that thePgtgenome sequence is useful in constructingPstphysical maps.

scholarly journals Race Composition of Puccinia striiformis f. sp. tritici in Tibet, China

Plant Disease ◽  
2012 ◽  
Vol 96 (11) ◽  
pp. 1615-1620 ◽  
Author(s):  
Xiaoping Hu ◽  
Jiaojiao Li ◽  
Yating Wang ◽  
Baotong Wang ◽  
Qiang Li ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Winter Wheat ◽  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Wheat Stripe Rust ◽  
Inoculum Source ◽  
Rust Pathogen ◽  
Fungal Populations ◽  
Standard Set ◽  
New Races

In Tibet, China, wheat stripe rust (caused by Puccinia striiformis f. sp. tritici) has recently become one of the most destructive diseases on winter wheat. To identify races of the pathogen in Tibet, 261 isolates were obtained in 2010 and tested on seedlings of a standard set of 19 wheat indicator genotypes. Of the 261 isolates, 248 were identified as members of 19 known races (CYR17, CYR20, CYR21, CYR22, CYR23, CYR29, CYR31, CYR32, CYR33, Lov13-6, Su11-1, Su11-2, Su11-3, Su11-4, Su11-5, Su11-6, Su11-7, Su11-8, and Su11-13), and 13 identified as representatives of 4 new races. CYR32 and CYR33 were the most predominant. The number of races and their frequencies in Tibet were more similar to the fungal populations in Sichuan and Gansu provinces than to those in Yunnan, Qinghai, and Shaanxi provinces. The results suggest that Tibet is also a possible center of inoculum source and genetic variation for the stripe rust pathogen in addition to Sichuan and Gansu.

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