scholarly journals Complete Genome Sequence Data of rare Actinomycetes Strain, Saccharothrix texasensis strain 6-C, a Biological Control Agent for Potato Late Blight

Author(s):  
Shun Feng ◽  
Yongfei Jian ◽  
Liang Jin ◽  
Shicai Tang ◽  
Zhengguo Li

A rare actinomycetes strain of Saccharothrix texasensis strain 6-C, has been isolated from the potato rhizosphere and it was shown to act as a biological control agent to potato late blight. It is also the first report on Saccharothrix inhibiting P. infestans. Here, we present the complete genome data of S. texasensis strain 6-C, assembled by sequencing reads obtained by both PacBio and Illumina technologies with annotation. The final assembled genome length is 9,045,220 bp, with no gaps, no plasmid, and its GC content is 72.39%. Nine non-ribosomal peptide synthetase (NRPS), five type I polyketide synthase (T1PKS), four terpene and three lanthipeptide gene clusters were identified in the genome, which would be likely to encode lots of antimicrobial active substances to help host plants against disease. This genome sequence could contribute to investigations of the molecular basis underlying the biocontrol activity of this Saccharothrix strain.

2020 ◽  
Vol 33 (12) ◽  
pp. 1451-1453
Author(s):  
Yoshiteru Noutoshi ◽  
Atsushi Toyoda ◽  
Tomoya Ishii ◽  
Kirara Saito ◽  
Megumi Watanabe ◽  
...  

Crown gall disease in grapevine is caused by pathogenic strains of Rhizobium vitis with a tumor-inducing (Ti) plasmids. A nonpathogenic strain, VAR03-1 of R. vitis, has been isolated from the grapevine root of nursery stock and it was shown to act as a biological control agent to crown gall disease. Its disease-suppressive effect was observed even when it was coinoculated with the pathogen in a 1:1 ratio. Here, we present the complete genome data of R. vitis VAR03-1, assembled by sequencing reads obtained by both PacBio and Illumina technologies with annotation. This genome sequence could contribute to investigations of the molecular basis underlying the biocontrol activity as well as the root-colonization ability of this bacterial strain. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .


2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Cesar D. Solorzano ◽  
Hamed K. Abbas ◽  
Robert M. Zablotowicz ◽  
Perng-Kuang Chang ◽  
Walker A. Jones

A nontoxigenicAspergillus flavusstrain, K49, is currently being tested as a biological control agent in corn fields in the Mississippi Delta. However, little is known about the overall genetic diversity ofA. flavusfrom year to year in corn fields and specifically in Mississippi. Our objective was to assess the genetic variability ofA. flavusisolates from different seasons, inoculum sources, and years, from a no-till corn field. Of the 175A. flavusisolates examined, 74 and 97 had the typicalnorB-cypAtype I (1.5 kb) and type II (1.0 kb) deletion patterns, respectively. Variability in the sequence of theomtAgene of the majority of the field isolates (n=118) was compared to strain K49. High levels of haplotypic diversity (24omtAhaplotypes; Hd = 0.61 ± 0.04) were found. Among the 24 haplotypes, two were predominant, H1 (n=71), which consists of mostly toxigenic isolates, and H49 (n=18), which consists of mostly atoxigenic isolates including K49. Toxigenic isolates were prevalent (60%) in this natural population. Nonetheless, about 15% of the population likely shared the same ancestral origin with K49. This study provides valuable information on the diversity ofA. flavus. This knowledge can be further used to develop additional biological control strains.


Insects ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 482
Author(s):  
Modesto del Pino ◽  
Juan Ramón Gallego ◽  
Estrella Hernández Suárez ◽  
Tomás Cabello

Trichogramma achaeae Nagaraja and Nagarkatti (Hymenoptera: Trichogrammatidae) is currently used as biological control agent for several lepidopteran pests. Knowledge of thermal requirements is essential to optimize its rearing procedures and inundative releases. The biological characteristics and two-sex life table parameters of T. achaeae were determined at five constant temperatures (15, 20, 25, 30, and 35 °C) using Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) eggs. T. achaeae was able to develop and survive from 15 °C to 30 °C, but not at 35 °C. Temperature significantly affected the preadult development time and adult longevity, decreasing when temperature increased from 15 °C to 30 °C. Temperature significantly altered the sex ratio, being female biased between 15 °C and 25 °C. Age-stage, two-sex life table analysis indicated that net reproductive rate (R0) was highest at 20 °C. Both the intrinsic rate of increase (r) and finite rate of increase (λ) increased with increasing temperature, while the mean generation time (T) decreased significantly. In addition, functional response of T. achaeae was studied, being significantly affected by temperature and host egg density, displaying a Holling type-I at 15 °C and a Holling type-II at 25 °C. The relevance of these results is discussed for the use of T. achaeae as biological control agent.


2017 ◽  
Vol 5 (12) ◽  
Author(s):  
Sandeep K. Kushwaha ◽  
Ramesh R. Vetukuri ◽  
Laura J. Grenville-Briggs

ABSTRACT The oomycete Pythium periplocum is an aggressive mycoparasite of a number of plant pathogenic fungi and oomycetes and therefore has potential as a biological control agent. Here, we report the first draft genome sequence of P. periplocum, which comprises 35.89 Mb. It contains 1,043 scaffolds and 14,399 predicted protein-coding genes.


2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Andrea G. Albarracín Orio ◽  
Romina A. Tobares ◽  
Daniel A. Ducasse ◽  
Andrea M. Smania

Bacillus subtilis is a nonpathogenic bacterium that lives in soil and has long been used as biological control agent in agriculture. Here, we report the genome sequence of a B. subtilis strain isolated from rhizosphere of onion that shows strong biological activity against the soilborne fungal pathogen Setophoma terrestris .


2021 ◽  
Vol 10 (45) ◽  
Author(s):  
D. V. Poshvina ◽  
A. V. Vasilchenko ◽  
A. S. Vasilchenko

Here, we announce and describe the draft genome sequence of Pseudomonas brassicacearum UTMN3, which contains 40 contigs comprising 6,658,810 bp, with a GC content of 60.9%. The genome contains 5,825 protein-coding genes and 65 RNA-coding genes. The genome of UTMN3 contains several genes that are likely contributors to plant protection.


2017 ◽  
Vol 5 (21) ◽  
Author(s):  
Sandeep K. Kushwaha ◽  
Ramesh R. Vetukuri ◽  
Laura J. Grenville-Briggs

ABSTRACT The oomycete Pythium oligandrum is a mycoparasite and licenced biological control agent. Here, we report the draft genome sequence of P. oligandrum strain CBS 530.74, which is 36.80 Mb. It contains 341 scaffolds and 11,647 predicted protein-coding genes. As reported for plant-pathogenic Pythium species, RXLR-type effector sequences are absent.


Sign in / Sign up

Export Citation Format

Share Document