Impaired Cell-to-Cell Movement of Potato Virus Y in Pepper Plants Carrying the ya(pr21)Resistance Gene

Potato virus Y (PVY) is an economically important plant virus that infects Solanaceous crops such as tobacco and potato. To date, studies into the localization and movement of PVY in plants have been limited to detection of viral RNA or proteins ex vivo. Here, a PVY N605 isolate was tagged with green fluorescent protein (GFP), characterized and used for in vivo tracking. In Nicotiana tabacum cv. Xanthi, PVY N605-GFP was biologically comparable to nontagged PVY N605, stable through three plant-to-plant passages and persisted for four months in infected plants. GFP was detected before symptoms and fluorescence intensity correlated with PVY RNA concentrations. PVY N605-GFP provided in vivo tracking of long-distance movement, allowing estimation of the cell-to-cell movement rate of PVY in N. tabacum cv. Xanthi (7.1 ± 1.5 cells per hour). PVY N605-GFP was adequately stable in Solanum tuberosum cvs. Désirée and NahG-Désirée and able to infect S. tuberosum cvs. Bintje and Bea, Nicotiana benthamiana, and wild potato relatives. PVY N605-GFP is therefore a powerful tool for future studies of PVY-host interactions, such as functional analysis of viral and plant genes involved in viral movement.

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Impact of tobacco recessive resistance gene va on biological properties of Brazilian Potato virus Y (PVY) isolates

Plant Pathology ◽

10.1111/j.1365-3059.2011.02473.x ◽

2011 ◽

Vol 60 (6) ◽

pp. 1048-1054 ◽

Cited By ~ 2

Author(s):

C. Lacroix ◽

L. Glais ◽

J.-L. Verrier ◽

C. Charlier ◽

C. Lorencetti ◽

...

Keyword(s):

Resistance Gene ◽

Potato Virus ◽

Potato Virus Y ◽

Biological Properties ◽

Recessive Resistance ◽

Recessive Resistance Gene

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Potato Virus Y Resistance Gene, Ry chc, Mapped to the Distal End of Potato Chromosome 9

Euphytica ◽

10.1007/s10681-006-9090-y ◽

2006 ◽

Vol 149 (3) ◽

pp. 367-372 ◽

Cited By ~ 36

Author(s):

Masatoshi Sato ◽

Kazuko Nishikawa ◽

Kuninori Komura ◽

Kazuyoshi Hosaka

Keyword(s):

Resistance Gene ◽

Potato Virus ◽

Potato Virus Y ◽

Chromosome 9 ◽

Potato Chromosome

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Biological characterization of French Potato virus Y (PVY) isolates collected from PVY-susceptible or -resistant tobacco plants possessing the recessive resistance gene va

Plant Pathology ◽

10.1111/j.1365-3059.2010.02342.x ◽

2010 ◽

Vol 59 (6) ◽

pp. 1133-1143 ◽

Cited By ~ 18

Author(s):

C. Lacroix ◽

L. Glais ◽

C. Kerlan ◽

J.-L. Verrier ◽

E. Jacquot

Keyword(s):

Resistance Gene ◽

Potato Virus ◽

Potato Virus Y ◽

Biological Characterization ◽

Recessive Resistance ◽

Tobacco Plants ◽

Recessive Resistance Gene

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Different Mutations in the Genome-Linked Protein VPg of Potato virus Y Confer Virulence on the pvr23 Resistance in Pepper

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-19-0557 ◽

2006 ◽

Vol 19 (5) ◽

pp. 557-563 ◽

Cited By ~ 86

Author(s):

Valérie Ayme ◽

Sylvie Souche ◽

Carole Caranta ◽

Mireille Jacquemond ◽

Joël Chadœuf ◽

...

Keyword(s):

Amino Acid ◽

Genetic Drift ◽

Potato Virus ◽

High Frequency ◽

Potato Virus Y ◽

Relative Fitness ◽

Amino Acid Substitutions ◽

Nucleotide Substitutions ◽

Single Nucleotide ◽

Pepper Plants

Five different amino acid substitutions in the VPg of Potato virus Y were shown to be independently responsible for virulence toward pvr23 resistance gene of pepper. A consequence of these multiple mutations toward virulence involving single nucleotide substitutions is a particularly high frequency of resistance breaking (37% of inoculated plants from the first inoculation) and suggests a potentially low durability of pvr23 resistance. These five mutants were observed with significantly different frequencies, one of them being overrepresented. Genetic drift alone could not explain the observed distribution of virulent mutants. More plausible scenarios were obtained by taking into account either the relative substitution rates, the relative fitness of the mutants in pvr23 pepper plants, or both.

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Effect of temperature on resistance to Potato virus Y in potato cultivars carrying the resistance gene Ry chc

Plant Pathology ◽

10.1111/ppa.12862 ◽

2018 ◽

Vol 67 (7) ◽

pp. 1629-1635 ◽

Cited By ~ 4

Author(s):

T. Ohki ◽

M. Sano ◽

K. Asano ◽

T. Nakayama ◽

T. Maoka

Keyword(s):

Resistance Gene ◽

Potato Virus ◽

Potato Virus Y ◽

Potato Cultivars ◽

Effect Of Temperature

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Molecular mapping of the potato virus Y resistance gene Rysto in potato

Theoretical and Applied Genetics ◽

10.1007/s001220050400 ◽

1997 ◽

Vol 94 (2) ◽

pp. 198-203 ◽

Cited By ~ 78

Author(s):

G. Brigneti ◽

J. Garcia-Mas ◽

D. C. Baulcombe

Keyword(s):

Resistance Gene ◽

Potato Virus ◽

Potato Virus Y ◽

Molecular Mapping

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A Single Amino Acid Change in Viral Genome-Associated Protein of Potato Virus Y Correlates with Resistance Breaking in ‘Virgin A Mutant’ Tobacco

Phytopathology ◽

10.1094/phyto.1999.89.2.118 ◽

1999 ◽

Vol 89 (2) ◽

pp. 118-123 ◽

Cited By ~ 56

Author(s):

Chikara Masuta ◽

Mitsuyo Nishimura ◽

Hiroshi Morishita ◽

Tatsuji Hataya

Keyword(s):

Amino Acid ◽

Potato Virus ◽

Viral Genome ◽

Potato Virus Y ◽

Cell Movement ◽

Amino Acid Sequences ◽

Single Amino Acid ◽

Viral Gene ◽

Resistance Breaking ◽

Single Amino Acid Change

Tobacco cultivar Virgin A Mutant (VAM) is reported to have the recessive potyvirus resistance gene va. Varied levels of resistance were observed in VAM plants inoculated with Japanese potato virus Y (PVY) isolates. VAM was highly resistant to most of the PVY isolates tested and tolerant to three necrotic strain isolates of PVY-T. Based on data obtained from tissue printing and press blotting, the resistance appeared to be mainly at the level of cell-to-cell movement. PVY replicated in VAM proto-plasts, but the replication was 30% lower than in susceptible tobacco, suggesting that impairment of replication also contributes to resistance. To identify the viral gene product or products involved in VAM resistance, we isolated spontaneous resistance-breaking mutants by passing vein-banding (O strain) isolates several times through VAM plants. By comparing the amino acid sequences of the mutants with their original isolates, we identified a single amino acid substitution in the viral genome-associated protein (VPg) domain that is correlated with VAM resistance breaking. Together, these results suggest that, in addition to its role in replication, VPg plays an important role in the cell-to-cell movement of PVY.

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