scholarly journals First Report of Bean pod mottle virus in Soybean in Iran

Plant Disease ◽  
2005 ◽  
Vol 89 (7) ◽  
pp. 775-775 ◽  
Author(s):  
N. Shahraeen ◽  
T. Ghotbi ◽  
M. Salati ◽  
A. Sahandi
Keyword(s):  
Visual Assessment ◽  
Leaf Beetle ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Minor Importance ◽  
Bean Pod Mottle Virus ◽  
Trifoliate Leaf ◽  
First Report ◽  
Das Elisa ◽  
Green Stem

Soybean (Glycine max (L.) Merr.) has been increasing in importance and acreage for the past 5 years in Iran and is now planted on approximately 108,000 ha. Previous surveys in Iran of viruses infecting soybean failed to identify Bean pod mottle virus (BPMV), but the incidence of other common viruses of soybean in the field has been reported (1). During October 2004, symptoms characteristic of those caused by BPMV including mosaic, puckering of trifoliate leaves, and delayed maturity of stems or green stems were observed in soybean fields in the Takhti Mahaleh, Versen, and Hashemabad areas located in the Gorgan Province. Sporadic incidence of plants infected with BPMV has been usually of minor importance to growers. Symptoms were often overlooked or considered to be physiological disorders. A visual assessment was made to determine incidence of green stem in the commonly grown soybean cv. Sahar. Forty soybean plants showing symptoms of crinkling, mottling, green stem, and retaining green leaves were sampled by collecting one trifoliate leaf near the top of the plant. All samples were tested in parallel for BPMV using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). BPMV was detected in 40% of the samples. Seven of the samples shown to be infected with BPMV using DAS-ELISA were mechanically (2) transferred to soybean seedlings in the greenhouse. These plants developed systemic mottle symptoms typical of those caused by BPMV and tested positive for BPMV using DAS-ELISA. The distribution of BPMV within soybean-growing regions, exploration of potential virus reservoirs, and economic impact of this virus have yet to be determined. There is no published report on the presence of potential BPMV vectors including the bean leaf beetle (Cerotoma trifurcata) from soybean fields in Iran. To our knowledge, this is the first report of BPMV in Iran. References: (1) A. R. Golnaraghi et al. Plant Dis.88:1069, 2004. (2) R. Louie et al. Plant Dis.84:1133, 2000.

scholarly journals First Report of Bean pod mottle virus in Soybean in Alabama

Plant Disease ◽  
2005 ◽  
Vol 89 (1) ◽  
pp. 108-108 ◽  
Author(s):  
E. J. Sikora ◽  
J. F. Murphy
Keyword(s):  
Visual Assessment ◽  
Leaf Beetle ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bean Pod Mottle Virus ◽  
Trifoliate Leaf ◽  
First Report ◽  
Soybean Seedlings ◽  
Das Elisa ◽  
Green Stem

During October 2003, soybean (Glycine max (L.) Merr.) plants showing symptoms of delayed maturity of stems, or green stem, were observed in a soybean cultivar trial on Dee River Ranch in Pickens County, Alabama. Symptoms were characteristic of those caused by Bean pod mottle virus (BPMV). BPMV infections have been identified in other southern states, including Arkansas, Louisiana, and neighboring Mississippi, but had not yet been identified in Alabama (1,2,3). In this study, a cultivar trial was established as a nonreplicated strip test to evaluate the performance of nongenetically modified soybean cultivars in high-pH soils. The trial consisted of 12 maturity group V cultivars planted side by side in 24-row plots approximately 1 km long. The cultivars consisted of Anand, Asgrow 5547, Asgrow 5944, Delta King 5995, Deltapine 4748, Deltapine 5110, Deltapine 5989, Essex, Hutcheson, Pioneer 9594, Pioneer 9597, and USG5601T. During the season, a known vector of BPMV, the bean leaf beetle (Cerotoma trifurcate Forster), was identified in the plots (4). On 10 October, the majority of plants in the trial had senesced; however, it was observed that plants of 6 of the 12 cultivars were showing symptoms of green stem typical of BPMV infection. A visual assessment was taken to determine incidence of green stem for Asgrow 5547, Delta King 5995, Deltapine 5110, Deltapine 5989, Pioneer 9594, and USG5601T. Incidence between 1 and 5% was observed for Delta King 5995, Deltapine 5989, and Pioneer 9594. Incidence of less than 1% was observed for Asgrow 5547, Deltapine 5110, and USG5601T. Twenty soybean plants showing symptoms of green stem and retaining green leaves were sampled from each of the six cultivars by collecting one trifoliate leaf near the top of the plant. All samples were tested for BPMV using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) according to the manufacturer's instructions (Agdia, Inc., Elkart, IN). BPMV was detected in 30% of Deltapine 5989, 10% of Delta King 5995, and 45% of Pioneer 9594 plants. BPMV was not detected in Asgrow 5547, Deltapine 5110, and USG5601T. Ten of the samples shown to be infected with BPMV using DAS-ELISA were mechanically transferred to soybean seedlings in the greenhouse. These plants developed systemic mottle symptoms typical of those caused by BPMV and tested positive for the virus BPMV using DAS-ELISA. To our knowledge, this is the first report of BPMV in Alabama. References: (1) N. S. Horn et al. LA. Agric. 13:12, 1970. (2) H. N. Pitre et al. Plant Dis. Rep. 63:419, 1979. (3) J. P. Ross. Plant Dis. Rep. 47:1049, 1963. (4) H. J. Walters. (Abstr.) Phytopathology 48:346, 1958.

scholarly journals First Report of Bean pod mottle virus in Soybean in Canada

Plant Disease ◽  
2002 ◽  
Vol 86 (3) ◽  
pp. 330-330 ◽  
Author(s):  
R. Michelutti ◽  
J. C. Tu ◽  
D. W. A. Hunt ◽  
D. Gagnier ◽  
T. R. Anderson ◽  
...  
Keyword(s):  
Soybean Mosaic Virus ◽  
Mottle Virus ◽  
Tobacco Streak Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Streak Virus ◽  
Growth Stages ◽  
Essex County ◽  
Bean Pod Mottle Virus ◽  
First Report ◽  
Soybean Plants

In 2001, soybean fields were surveyed to determine the incidence of viruses because soybean aphids (Aphis glycines Matsamura), known to transmit Soybean mosaic virus (SMV) (2), were found in Ontario. In addition, bean leaf beetle (Cerotoma trifurcata Forster) was found during 2000 to be contaminated with Bean pod mottle virus (BPMV), although soybean plants, on which the beetles were feeding, tested negative (3). In the current survey, young soybean leaves were selected at random in July and August from 20 plants per site at growth stages R4 to R5 (1) from 415 sites representing the entire soybean-producing area in Ontario. Samples were maintained under cool conditions until received at the laboratory, where they were promptly processed. A combined sub-sample was obtained from the 20 plants per site. The 415 sub-samples were tested for SMV, BPMV, Tobacco ringspot virus (TRSV), and Tobacco streak virus (TSV) using polyclonal antibody kits for double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) (Agdia Inc., Elkart, IN). The ELISA plates were read with a plate reader (MRX, Dynex Technologies Inc., Chantilly, VA), and results were analyzed using ELISA software (Leading Edge Research, Merrickville, Ontario) and compared positive and negative controls (Agdia). TRSV was detected in one sample from Essex County and another sample from Middlesex County. SMV, BPMV, and TSV were not found in commercial soybean fields. However, SMV and BPMV were found in samples originating from two soybean breeding nurseries, one in Essex County and one in Kent County. Seedlings of soybean cv. Williams 82 were inoculated in the greenhouse with sap from leaf samples that tested positive for BPMV. Leaves of plants that developed mosaic symptoms were retested using ELISA and confirmed to be positive for BPMV. SMV and TRSV have been found previously in commercial soybean fields in Ontario (4). To our knowledge, this is the first report of BPMV on soybean plants in Canada. References: (1) W. R. Fehr et al. Merr. Crop. Sci. 11:929, 1971. (2) J. H. Hill et al. Plant Dis. 85:561, 2001. (3) A. U. Tenuta. Crop Pest. 5 (11):8, 2000. (4) J. C. Tu. Can. J. Plant Sci. 66:491, 1986.

scholarly journals First Report of Bean pod mottle virus in Soybean in Ohio

Plant Disease ◽  
2001 ◽  
Vol 85 (9) ◽  
pp. 1029-1029 ◽  
Author(s):  
A. E. Dorrance ◽  
D. T. Gordon ◽  
A. F. Schmitthenner ◽  
C. R. Grau
Keyword(s):  
Host Range ◽  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Mottle Virus ◽  
Yellow Mosaic Virus ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bean Pod Mottle Virus ◽  
First Report ◽  
Soybean Leaf

Soybean has been increasing in importance and acreage over wheat and corn for the past decade in Ohio and is now planted on 4.5 million acres. Previous surveys in Ohio of viruses infecting soybean failed to identify Bean pod mottle virus (BPMV) and soybean virus diseases have rarely caused economic losses (1). During 1999, producers in Ohio noticed virus-like symptoms in soybeans in a few isolated locations. Soybeans with green stems, undersized and “turned up pods” were collected from Union, Wood and Wyandot Counties during October 1999 and soybeans with crinkled, mottled leaves were collected in Henry, Licking and Sandusky during August 2000. Five to six plants were collected from a single field from each county each year. In 1999, samples were sent to the University of Wisconsin-Madison, where one symptomatic leaflet/sample was ground in 3 ml of chilled phosphate buffered saline (pH 7.2). Leaf sap was placed in 1.5-ml centrifuge tubes and stored at 4°C for 24 h. Sap was assayed for the presence of BPMV using an alkaline phosphatase-labeled double-antibody sandwich enzyme-linked immunosorbent assay (DAS ELISA) for BPMV (AgDia Inc., Elkhart, IN). All samples tested were positive for BPMV. Samples collected in 1999 were also maintained at The Ohio State University in Harosoy soybean and in 2000 assayed serologically along with samples collected in 2000 for BPMV and Soybean mosaic virus (SMV) by ELISA and for Tobacco ringspot virus (TRSV) and Bean yellow mosaic virus (BYMV) by a host-range symptom assay; SMV, BYMV and TRSV had been identified from soybean in previous Ohio surveys. Soybean leaf samples were assayed using F(ab′)2-Protein A ELISA with antiserum prepared in 1968 to a southern U.S. isolate of BPMV and to an Ohio isolate of Soybean mosaic virus (SMV) prepared in 1967, both stored at −20°C. Diseased and non-symptomatic soybean leaf samples were ground in 4 ml 0.025M Tris pH 8.0, 0.015M NaCl and 0.05% Tween 20. Extracts were tested for BPMV and SMV by ELISA following a protocol described elsewhere (2). All of the samples collected during 1999 and maintained in the greenhouse tested positive for both BPMV and SMV while all of those samples collected during 2000 tested positive for BPMV and negative for SMV. Host-range symptom assays were conducted with leaf extracts prepared by grinding 1 g tissue:10 ml potassium phosphate buffer, pH 7.0. Extracts were inoculated by leaf rub method to Harosoy soybean, Phaseolus vulgaris cvs. Red Kidney and Bountiful, cowpea, and cucumber. The host-range symptom assays of both the 1999 and 2000 samples were negative for TRSV and BYMV; cowpea failed to express local lesions and cucumber systemic mosaic characteristic of TRSV infection and the two Phaseolus cultivars the yellow mosaic characteristic of BYMV infection. These results indicate that both BPMV and SMV were present in the samples in 1999 but only BPMV in 2000. The distribution of BPMV within Ohio and economic impact of this virus have yet to be determined. This is the first report of BPMV in Ohio. References: (1) A. F. Schmitthenner and D. T. Gordon. Phytopathology 59:1048, 1969. (2) R. Louie et al. Plant Dis. 84:1133–1139, 2000.

scholarly journals First Report of Cucurbit aphid-borne yellows virus in Iran Causing Yellows on Four Cucurbit Crops

Plant Disease ◽  
2006 ◽  
Vol 90 (4) ◽  
pp. 526-526 ◽  
Author(s):  
K. Bananej ◽  
C. Desbiez ◽  
C. Wipf-Scheibel ◽  
I. Vahdat ◽  
A. Kheyr-Pour ◽  
...  
Keyword(s):  
Citrullus Lanatus ◽  
Healthy Plant ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
First Report ◽  
Chain Reactions ◽  
Reference Isolate ◽  
Sigma Chemical ◽  
International Mycological Institute ◽  
Das Elisa

A survey was conducted from 2001 to 2004 in the major cucurbit-growing areas in Iran to reassess the relative incidence of cucurbit viruses. Severe yellowing symptoms were observed frequently on older leaves of cucurbit plants in various regions in outdoor crops, suggesting the presence of Cucurbit aphid-borne yellows virus (CABYV, genus Polerovirus, family Luteoviridae) (1,2). Leaf samples (n = 1019) were collected from plants of melon (Cucumis melo L.), cucumber (C. sativus L.), squash (Cucurbita sp.), and watermelon (Citrullus lanatus L.) showing various virus-like symptoms (mosaic, leaf deformation, yellowing). All samples, collected from 15 provinces, were screened for the presence of CABYV by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with IgGs and alkaline phosphatase-conjugated IgGs against a CABYV reference isolate (1). Of the 1,019 samples tested, 471 were positive for CABYV using DAS-ELISA. Some of the positive samples had typical severe yellowing symptoms while symptoms in other samples were masked by mosaic or leaf deformations caused by other viruses frequently found in mixed infections (data not shown). During the entire survey, CABYV was detected by DAS-ELISA in 201 of 503 melon samples, 72 of 129 cucumber samples, 158 of 249 squash samples, and 40 of 138 watermelon samples. These results indicate that CABYV is widely distributed on four cucurbit species in the major growing areas of Iran. In order to confirm CABYV identification, total RNA extracts (TRI-Reagent, Sigma Chemical, St Louis, MO) were obtained from 25 samples that were positive using DAS-ELISA originating from Khorasan (n = 4), Esfahan (n = 6), Teheran (n = 3), Hormozgan (n = 4), Azerbaiejan-E-Sharqi (n = 4), and Kerman (n = 4). Reverse transcription-polymerase chain reactions (RT-PCR) were carried out using forward (5′-CGCGTGGTTGTGG-TCAACCC-3′) and reverse (5′-CCYGCAACCGAGGAAGATCC-3′) primers designed in conserved regions of the coat protein gene according to the sequence of a CABYV reference isolate (3) and three other unpublished CABYV sequences. RT-PCR experiments yielded an expected 479-bp product similar to the fragment amplified with extracts from the reference isolate. No amplification of the product occurred from healthy plant extracts. To our knowledge, this is the first report of the occurrence of CABYV in Iran on various cucurbit species. The high frequency (46.2%) with which CABYV was detected in the samples assayed indicates that this virus is one of the most common virus infecting cucurbits in Iran. References: (1) H. Lecoq et al. Plant Pathol. 41:749, 1992 (2) M. A. Mayo and C. J. D'Arcy. Page 15 in: The Luteoviridae. H. G. Smith and H. Barker, eds. CAB International Mycological Institute, Wallingford, UK, 1999. (3) H. Guilley et al. Virology 202:1012, 1994.

scholarly journals Quantifying the Within-Field Temporal and Spatial Dynamics of Bean pod mottle virus in Soybean

Plant Disease ◽  
2011 ◽  
Vol 95 (2) ◽  
pp. 126-136 ◽  
Author(s):  
E. Byamukama ◽  
A. E. Robertson ◽  
F. W. Nutter
Keyword(s):  
Point Source ◽  
Spatial Dynamics ◽  
Leaf Beetle ◽  
Mottle Virus ◽  
Growth Stages ◽  
Spatial Analyses ◽  
Bean Pod Mottle Virus ◽  
Beetle Population ◽  
Bean Leaf Beetle ◽  
Temporal And Spatial

The prevalence and incidence of Bean pod mottle virus (BPMV) have been reported to be on the increase in the United States but little is known about the temporal and spatial dynamics of this virus within soybean (Glycine max) fields. A quadrat-based sampling method was developed to quantify the within-field spread of BPMV in soybean in 2006 and 2007. Twenty-five 30-cm-long quadrats were established within each row of soybean in field plots consisting of six rows, each 7.6 m long and spaced 0.76 m apart. Four treatments were used to influence the temporal and spatial dynamics of BPMV epidemics. Treatments were: (i) establishment of a point source of BPMV inoculum within soybean plots; (ii) lambda-cyhalothrin insecticide applied at the V1 and R2 growth stages; (iii) establishment of a BPMV inoculum point source, plus the application of foliar insecticide sprays at the V1 and R2 growth stages; and (iv) a nontreated, noninoculated control. All quadrats (census) were sampled beginning 25 days after planting; sampling continued every 8 to 11 days until plants were senescent. Sap from leaf samples was extracted and tested for BPMV by enzyme-linked immunosorbent assay. The incidence of BPMV per treatment was plotted against time to produce BPMV incidence curves for temporal analyses. In addition, positions of BPMV-positive quadrats were mapped for spatial analyses. BPMV was detected within soybean plots on the first sampling date in 2006 (30 May) and on the second sampling date in 2007 (21 June). The rate of BPMV temporal spread within treatments ranged from 0.11 to 0.13 logits/day in 2006 and from 0.05 to 0.07 logits/day in 2007. Doubling times for BPMV incidence among treatments ranged from 5.4 to 6.4 days in 2006 and from 10.0 to 14.1 days in 2007. Soybean plots that had the earliest dates of BPMV detection within quadrats (x) also had the highest BPMV incidence (y) at the end of the growing season (R2 = 66.5 and 70.4% for 2006 and 2007, respectively). Spatial analyses using ordinary runs, black-white join-counts, and spatial autocorrelation revealed highly aggregated spatial patterns of BPMV-infected quadrats over time. Bean leaf beetle population densities were linearly related to BPMV incidence (P < 0.0001) in both years, indicating that BPMV epidemics were greatly influenced by bean leaf beetle population density. To our knowledge, this is the first study to quantify the seasonal temporal and spatial dynamics of BPMV spread within soybean.

scholarly journals First Report of Sharka Disease Caused by Plum Pox Virus in Lithuania

Plant Disease ◽  
1998 ◽  
Vol 82 (12) ◽  
pp. 1405-1405 ◽  
Author(s):  
J. Staniulis ◽  
J. Stankiene ◽  
K. Sasnauskas ◽  
A. Dargeviciute
Keyword(s):  
Coat Protein ◽  
Plant Protection ◽  
Virus Disease ◽  
Pcr Amplification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plum Pox Virus ◽  
Rt Pcr ◽  
First Report ◽  
Das Elisa ◽  
Sharka Disease

Plum pox (sharka) disease caused by plum pox potyvirus (PPV) is considered the most important virus disease of stone fruit trees in Europe and the Mediterranean region. Nearly all those countries that produce stone fruits are affected (3). The causal virus of the disease is a European Plant Protection Organization A2 quarantine pathogen. Symptoms of leaf mottling, diffuse chlorotic spots, rings, and vein banding of varied intensity characteristic for plum pox virus infection were observed in the plum (Prunus domestica) orchard tree collection of the Lithuanian Institute of Horticulture in Babtai in 1996. Presence of this virus in the diseased trees was confirmed by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) with kits from BIOREBA (Reinach, Switzerland) and by polyclonal antibodies raised against a Moldavian isolate of PPV courtesy of T. D. Verderevskaya (Institute of Horticulture, Kishinev, Moldova). ELISAs with both sources of antiserum were positive for presence of PPV. Electron microscopy revealed the presence of potyvirus-like particles averaging 770 nm in extracts of mechanically inoculated plants of Chenopodium foetidum (chlorotic LL [local lesions]) and Pisum sativum cvs. Rainiai and Citron (mottling). For molecular diagnosis and characterization of this isolate, PPV-971, reverse transcription-polymerase chain reaction (RT-PCR) was employed. Total RNA from the leaves of infected pea was isolated as described (2). High molecular weight RNA selectively precipitated with 2 M lithium chloride was used for RT-PCR amplification of the coat protein encoding sequence by use of specific primers complementary to 5′ and 3′ parts of PPV coat protein L1 (GenBank accession no. X81081). Amino acid sequence comparison with GenBank data indicated 98.2% similarity with coat protein of PPV potyvirus isolated by E. Mais et al. (accession no. X81083) and 97.3% with PPV strain Rankovic (1).The specific DNA fragment, corresponding to predicted coat protein sequence size, was cloned into Escherichia coli pUC57 for DNA sequencing. Expression of the cloned sequence in bacteria and yeast expression systems is under investigation. The presence of PPV in plum trees in the 9-year-old collection at Babtai was confirmed by DAS-ELISA in 1997 and again in 1998. PPV was then detected in 20% of symptomatic trees of three cultivars. The Lithuanian PPV isolate reacted positively with “universal” Mab.5b and with a Mab (Mab.4DG5) specific for PPV-D. No reaction was observed with Mabs specific for PPV-M (Mab.AL), PPV-C (Mab.AC and Mab.TUV), and PPV-El Amar (Mab.EA24). PPV-971 seems to be a typical member of the less aggressive Dideron strain cluster of PPV (D. Boscia, personal communication). This is the first report of PPV in Lithuania and confirms the necessity for continuing the precautionary measures established in this country for indexing of nursery plum trees used for graft propagation. References: (1) S. Lain et al. Virus Res. 13:157, 1989. (2) J. Logemann et al. Anal. Biochem. 163:16, 1987. (3) M. Nemeth. OEPP/EPPO Bull. 24:525, 1994.

scholarly journals First Report of Xylella fastidiosa Infecting Citrus in Costa Rica

Plant Disease ◽  
2005 ◽  
Vol 89 (6) ◽  
pp. 687-687 ◽  
Author(s):  
E. Aguilar ◽  
W. Villalobos ◽  
L. Moreira ◽  
C. M. Rodríguez ◽  
E. W. Kitajima ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Costa Rica ◽  
Sweet Orange ◽  
Enzyme Linked Immunosorbent Assay ◽  
Negative Bacterium ◽  
Gram Negative ◽  
First Report ◽  
Leaf Petiole ◽  
Orange Trees ◽  
Das Elisa

Citrus variegated chlorosis (CVC) is an important disease mainly of sweet orange (Citrus sinensis (L.) Osbeck) cultivars. It was first described in Brazil in the state of Sā Paulo in 1987 (4). The disease has spread to all Brazilian states that grow citrus and is affecting more than one-third of the orange trees grown in Brazil. CVC is caused by Xylella fastidiousa, a xylem-limited, gram-negative bacterium. During the last 4 years, symptoms including leaf interveinal chlorosis, stunting, canopy dieback, and hard and undersized fruits, similar to those caused by CVC (3), appeared in sweet orange trees used as shade plants for coffee plantations and as fence posts in Costa Rica. Necrotic lesions on the abaxial side of the leaves as reported in Brazil were rarely observed. Leaf petiole samples from 25 symptomatic sweet orange trees reacted positively with a X fastidiosa-specific antiserum (AGDIA Inc., Elkart, IN) in a double-sandwich antibody enzyme-linked immunosorbent assay (DAS-ELISA). A fastidious, gram-negative bacterium identified as X. fastidiosa using DAS-ELISA was isolated on perwinkle wilt (PW) medium plates (1) from citrus stems showing CVC symptoms, but not from asymptomatic trees. The isolated colonies were circular and opalescent with diameters of 2 to 3 mm and were clearly visible within 6 to 7 days after streaking. Petiole sections from symptomatic plants observed with scanning electron microscopy showed rod-shaped bacteria with rippled cell walls tightly packed in xylem vessels, as described for X. fastidiosa previously (2), and with transmission electron microscopy, the bacteria were morphologically similar to those reported previously for CVC (2). To our knowledge, this is the first report of X. fastidiosa associated with citrus in Costa Rica. References: (1) M. J. Davis et al. Curr. Microbiol. 6:309, 1981. (2) J. S. Hartung et al. Phytopathology 84:591, 1994. (3) R. F. Lee et al. Summa Phytopathol. 19:123, 1993. (4) V. Rossetti et al. 1990, C.R. Acad. Sci. (Paris) 310:345–349.

scholarly journals Occurrence of Chilli veinal mottle virus in Solanum aethiopicum in Tanzania

Plant Disease ◽  
2001 ◽  
Vol 85 (7) ◽  
pp. 801-801 ◽  
Author(s):  
R. Nono-Womdim ◽  
I. S. Swai ◽  
M. L. Chadha ◽  
K. Gebre-Selassie ◽  
G. Marchoux
Keyword(s):  
Green Peach Aphid ◽  
Disease Incidence ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Natural Occurrence ◽  
Solanum Aethiopicum ◽  
Field Samples ◽  
Arusha Region ◽  
Chilli Veinal Mottle Virus ◽  
Das Elisa

African eggplant, or garden egg (Solanum aethiopicum) is an important vegetable in most sub-Saharan African countries. Since June 1997, viral symptoms, including mosaic, vein clearing, and stunting, have been observed on several crops of African eggplant cv. Tengeru White at a number of sites in the Arusha region of northern Tanzania. Field inspections revealed disease incidence ranging from 50 to 90%. During the same period, high populations of the green peach aphid Myzus persicae were observed in affected crops of African eggplant. These aphids were also found to reproduce in African eggplants. Flexuous, rodshaped virus-like particles, approximately 750 nm long and 12 nm wide, were found in electron microscope leaf dips from field samples of naturally affected African eggplants. The particle size suggested a species of Potyviridae. Thus, 20 field-infected samples of S. aethiopicum (randomly collected from four farms) were assayed in double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for the presence of Potato virus Y (PVY) and Pepper veinal mottle virus (PVMV), known to infect tomato and other solanaceous crops in the region (2). However, all samples gave negative results. Further DAS-ELISA were performed with the same extracts from naturally infected plants of S. aethiopicum with antisera directed against Tobacco etch virus, Tobacco vein mottling virus, Pepper mottle virus, and Chilli veinal mottle virus (ChiVMV). All 20 samples were positive only for ChiVMV. ChiVMV, a single-stranded RNA virus transmitted in a nonpersistent manner by several aphid species, is one of the most important viruses of pepper in Asia (1). To confirm DAS-ELISA results, an isolate of ChiVMV from African eggplant was transmitted by mechanical inoculations, resulting in disease on tobacco (Nicotiana tobacco cv. Xanthi nc), pepper (Capsicum annuum cv. Yolo Wonder), tomato (Lycopersicon esculentum cv. Tengeru 97), and African eggplant (S. aethiopicum cv. Tengeru White). Extracts from the inoculated plants tested positive for the presence of ChiVMV in DAS-ELISA. This mechanically transmitted isolate did not infect melon (Cucumis melo), cucumber (C. sativus), or cowpea (Vigna unguiculata), which are nonhosts of ChiVMV. To our knowledge, this is the first report of the natural occurrence of ChiVMV in African eggplant. References: (1) S. K. Green et al. PETRIA 9:332, 1999. (2) R. Nono-Womdim et al. J. S. Afr. Soc. Hort. Sci. 6:41–44, 1996.

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