Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia

Geranium leaves and stems with symptoms of bacterial blight were collected from commercial greenhouses during the last decade in Serbia. In total, 17 isolates with colony morphology typical for the genus Xanthomonas were characterized with pathogenicity, biochemical, serological, and molecular assays. All 17 isolates reacted positive in a polymerase chain reaction (PCR) using XcpM1 and XcpM2 primers specific for Xanthomonas hortorum pv. pelargonii. In pathogenicity tests on Pelargonium zonale (leaf and stem inoculation), all isolates caused typical symptoms on leaves starting 2 days after inoculation as sunken, water-soaked, irregular lesions, and 6 to 8 days after inoculation on stems as necrotic lesions also showing yellow exudate. Symptoms resulted in general wilting of inoculated plants 20 days after inoculation. Selected phenotypic tests indicated that all isolates showed the same results as described for the bacterium X. hortorum pv. pelargonii. Repetitive sequence-based PCR typing using BOX and ERIC revealed that all isolates showed two fingerprinting profiles but (GTG)5 and REP did not reveal differences. Multilocus sequence typing of partial sequences of rpoD, dnaK, fyuA, and gyrB genes of tested isolates and sequences obtained from GenBank of Xanthomonas pathovar pathotype strains did not reveal genetic variability among the isolates, showing the same gene sequence pattern.

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Prevalence and characteristics ofEscherichia coilserotype O157:H7 and other verotoxin-producingE. coliin healthy cattle

Epidemiology and Infection ◽

10.1017/s0950268800001424 ◽

1996 ◽

Vol 117 (2) ◽

pp. 251-257 ◽

Cited By ~ 60

Author(s):

M. Blanco ◽

J. E. Blanco ◽

J. Blanco ◽

E. A. Gonzalez ◽

A. Mora ◽

...

Keyword(s):

Escherichia Coli ◽

Polymerase Chain Reaction ◽

Gene Sequence ◽

Heterogeneous Population ◽

Chain Reaction ◽

E Coli ◽

Healthy Cattle ◽

Faecal Samples ◽

Polymerase Chain

SummaryFrom February to July of 1994, 328 faecal samples from 32 herds were collected and verotoxin-producingEscherichia coli(VTEC) found on 84% of the farms. The proportion of animals infected varied from 0–63%. VTEC were recovered from 52 (20%) of 257 cows and from 16 (23%) of 71 calves. Although the VTEC belonged to 25 different serogroups, 7 (O8. O20, O22, O77, O113, O126 and O162) accounted for 46% of strains. Nearly 45% of the 83 bovine VTEC strains belonged to serogroups associated with haemorrhagic colitis and haemolytic uraernic syndrome in humans. However, only 2 (2%) of 83 VTEC strains isolated from cattle belonged to enterohaemorrhagicE. coli(EHEC) serotypes (O26:H11 and O157:H7), and only 8 (10%) were positive for the attaching and effacingE. coli (eae)gene sequence. Polymerase chain reaction (PCR) showed that 17 (20%) of VTEC strains carried VT1 genes. 43 (52%) possessed VT2 genes, and 23 (28%) carried both VT1 and VT2 genes. Characterization of VTEC isolates revelated a heterogeneous population in terms of serogroup and toxin type in the positive herds. This study confirms that healthy cattle are a reservoir of VTEC, but, the absence ofeaegenes in most bovine VTEC strains suggests that they may be less virulent for humans thaneae-positive EHEC.

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Characterization of a new human apolipoprotein A-I Yame by direct sequencing of polymerase chain reaction-amplified DNA.

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)41957-1 ◽

1991 ◽

Vol 32 (8) ◽

pp. 1275-1280

Author(s):

Y Takada ◽

J Sasaki ◽

M Seki ◽

S Ogata ◽

Y Teranishi ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Direct Sequencing ◽

Chain Reaction ◽

Human Apolipoprotein ◽

Apolipoprotein A ◽

Polymerase Chain

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Characterization of Giardia duodenalis by polymerase-chain-reaction fingerprinting

Parasitology Research ◽

10.1007/s004360050474 ◽

1998 ◽

Vol 84 (9) ◽

pp. 707-714 ◽

Cited By ~ 58

Author(s):

Wieger L. Homan ◽

Margriet Gilsing ◽

Hafida Bentala ◽

Louis Limper ◽

Frans van Knapen

Keyword(s):

Polymerase Chain Reaction ◽

Giardia Duodenalis ◽

Chain Reaction ◽

Polymerase Chain

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Gene sequences of the pcpB gene of pentachlorophenol-degrading Sphingomonas chlorophenolica found in nondegrading bacteria

Canadian Journal of Microbiology ◽

10.1139/w98-055 ◽

1998 ◽

Vol 44 (7) ◽

pp. 667-675 ◽

Cited By ~ 6

Author(s):

Vandana M Saboo ◽

Michael A Gealt

Keyword(s):

Polymerase Chain Reaction ◽

Gene Sequence ◽

Acid Methyl Ester ◽

Solid Support ◽

Contaminated Site ◽

Chain Reaction ◽

Hybridization Data ◽

Acid Methyl ◽

Sphingomonas Chlorophenolica ◽

Polymerase Chain

Bacteria isolated from a pentachlorophenol (PCP) contaminated site grew in the presence of 50 µg PCP/mL but were not able to degrade it in either liquid medium or the presence of 1% sterile potting soil as a solid support. Probes developed using the gene sequence of PCP-4-monooxygenase (pcpB) from Sphingomonas chlorophenolica sp.nov hybridized to two separate isolates. Identification based on fatty acid methyl ester profiles (Sherlock), substrate utilization (BIOLOG), and 16S rRNA showed that the two strains were different from each other and from Sphingomonas chlorophenolica. Sequences from these isolates, amplified by polymerase chain reaction, confirmed the homology with pcpB. The presence of pcpB sequences in these nondegraders indicated that growth and hybridization data alone were insufficient for predicting degradation capability. Key words: pentachlorophenol, Sphingomonas chlorophenolica, pcpB gene, pentachlorophenol-4-monooxygenase.

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Characterization of chloramphenicol acetyltransferase gene by multiplex polymerase chain reaction in multidrug-resistant strains isolated from aquatic environments

Aquaculture ◽

10.1016/s0044-8486(02)00169-2 ◽

2003 ◽

Vol 217 (1-4) ◽

pp. 11-21 ◽

Cited By ~ 31

Author(s):

Min Ho Yoo ◽

Min-Do Huh ◽

Eun-heui Kim ◽

Hyoung-Ho Lee ◽

Hyun Do Jeong

Keyword(s):

Polymerase Chain Reaction ◽

Multiplex Polymerase Chain Reaction ◽

Multidrug Resistant ◽

Chloramphenicol Acetyltransferase ◽

Aquatic Environments ◽

Chain Reaction ◽

Resistant Strains ◽

Polymerase Chain ◽

Chloramphenicol Acetyltransferase Gene

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Rhodococcus equi Infections in Goats: Characterization of Virulence Plasmids

Veterinary Pathology ◽

10.1177/0300985817747327 ◽

2017 ◽

Vol 55 (2) ◽

pp. 273-276 ◽

Cited By ~ 8

Author(s):

Lauren W. Stranahan ◽

Quinci D. Plumlee ◽

Sara D. Lawhon ◽

Noah D. Cohen ◽

Laura K. Bryan

Keyword(s):

Polymerase Chain Reaction ◽

Lymph Nodes ◽

Rhodococcus Equi ◽

Caseous Lymphadenitis ◽

Chain Reaction ◽

Disseminated Infection ◽

Virulence Plasmids ◽

Visceral Organs ◽

Polymerase Chain

Rhodococcus equi is an uncommon cause of systemic pyogranulomatous infections in goats with macroscopic similarities to caseous lymphadenitis caused by Corynebacterium pseudotuberculosis. Caprine cases have previously been reported to be caused by avirulent R. equi strains. Six cases of R. equi infection in goats yielding 8 R. equi isolates were identified from 2000 to 2017. Lesions varied from bronchopneumonia, vertebral and humeral osteomyelitis, and subcutaneous abscesses, to disseminated infection involving the lungs, lymph nodes, and multiple visceral organs. Isolates of R. equi from infected goats were analyzed by polymerase chain reaction for R. equi virulence-associated plasmid ( vap) genes. Seven of 8 isolates carried the VapN plasmid, originally characterized in bovine isolates, while 1 isolate lacked virulence plasmids and was classified as avirulent. The VapN plasmid has not been described in isolates cultured from goats.

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