Gene sequences of the pcpB gene of pentachlorophenol-degrading Sphingomonas chlorophenolica found in nondegrading bacteria

1998 ◽  
Vol 44 (7) ◽  
pp. 667-675 ◽  
Author(s):  
Vandana M Saboo ◽  
Michael A Gealt
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Sequence ◽  
Acid Methyl Ester ◽  
Solid Support ◽  
Contaminated Site ◽  
Chain Reaction ◽  
Hybridization Data ◽  
Acid Methyl ◽  
Sphingomonas Chlorophenolica ◽  
Polymerase Chain

Bacteria isolated from a pentachlorophenol (PCP) contaminated site grew in the presence of 50 µg PCP/mL but were not able to degrade it in either liquid medium or the presence of 1% sterile potting soil as a solid support. Probes developed using the gene sequence of PCP-4-monooxygenase (pcpB) from Sphingomonas chlorophenolica sp.nov hybridized to two separate isolates. Identification based on fatty acid methyl ester profiles (Sherlock™), substrate utilization (BIOLOG™), and 16S rRNA showed that the two strains were different from each other and from Sphingomonas chlorophenolica. Sequences from these isolates, amplified by polymerase chain reaction, confirmed the homology with pcpB. The presence of pcpB sequences in these nondegraders indicated that growth and hybridization data alone were insufficient for predicting degradation capability. Key words: pentachlorophenol, Sphingomonas chlorophenolica, pcpB gene, pentachlorophenol-4-monooxygenase.

scholarly journals Detection of Transgene in Tilapia (Oreochromis niloticus) by Polymerase Chain Reaction (PCR) Technique

2016 ◽  
Vol 8 (2) ◽  
pp. 47-49
Author(s):  
PC Ray ◽  
MS Alam ◽  
MS Islam
Keyword(s):  
Growth Hormone ◽  
Polymerase Chain Reaction ◽  
Oreochromis Niloticus ◽  
Gene Sequence ◽  
Transgenic Fish ◽  
Specific Primer ◽  
Chain Reaction ◽  
Gh Gene ◽  
Polymerase Chain ◽  
Transgenic Tilapia

Polymerase Chain Reaction (PCR) technique using specific primer can be used to detect transgenes. The present study was undertaken to detect salmon growth hormone (GH) gene in transgenic tilapia (Oreochromis niloticus) by PCR. DNA was extracted from F1 Tilapia generated by crossing transgenic parents. Two primers were designed to amplify a part of the region of GH gene sequence, which was used to make transgenic tilapia. To confirm the specificity of the selected primer, PCR was performed on diluted DNAs, extracted from tilapia fin tissues. GH transgene sequences (1500 bp) were successfully amplified from transgenic fish in this study. The specificity of the primers was found to be high in detecting the salmon GH transgenes. The PCR-based method therefore, could be used for fast and easy screening of transgenic fish for this gene.J. Environ. Sci. & Natural Resources, 8(2): 47-49 2015

scholarly journals Prevalence and characteristics ofEscherichia coilserotype O157:H7 and other verotoxin-producingE. coliin healthy cattle

1996 ◽  
Vol 117 (2) ◽  
pp. 251-257 ◽  
Author(s):  
M. Blanco ◽  
J. E. Blanco ◽  
J. Blanco ◽  
E. A. Gonzalez ◽  
A. Mora ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Gene Sequence ◽  
Heterogeneous Population ◽  
Chain Reaction ◽  
E Coli ◽  
Healthy Cattle ◽  
Faecal Samples ◽  
Polymerase Chain

SummaryFrom February to July of 1994, 328 faecal samples from 32 herds were collected and verotoxin-producingEscherichia coli(VTEC) found on 84% of the farms. The proportion of animals infected varied from 0–63%. VTEC were recovered from 52 (20%) of 257 cows and from 16 (23%) of 71 calves. Although the VTEC belonged to 25 different serogroups, 7 (O8. O20, O22, O77, O113, O126 and O162) accounted for 46% of strains. Nearly 45% of the 83 bovine VTEC strains belonged to serogroups associated with haemorrhagic colitis and haemolytic uraernic syndrome in humans. However, only 2 (2%) of 83 VTEC strains isolated from cattle belonged to enterohaemorrhagicE. coli(EHEC) serotypes (O26:H11 and O157:H7), and only 8 (10%) were positive for the attaching and effacingE. coli (eae)gene sequence. Polymerase chain reaction (PCR) showed that 17 (20%) of VTEC strains carried VT1 genes. 43 (52%) possessed VT2 genes, and 23 (28%) carried both VT1 and VT2 genes. Characterization of VTEC isolates revelated a heterogeneous population in terms of serogroup and toxin type in the positive herds. This study confirms that healthy cattle are a reservoir of VTEC, but, the absence ofeaegenes in most bovine VTEC strains suggests that they may be less virulent for humans thaneae-positive EHEC.

scholarly journals Pseudoterranova Infestation Beneath Uterine Serosa

2021 ◽  
Vol 6 (4) ◽  
Keyword(s):  
Monoclonal Antibody ◽  
Polymerase Chain Reaction ◽  
Gene Sequence ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Total Hysterectomy ◽  
Pseudoterranova Decipiens ◽  
First Case ◽  
Polymerase Chain

A 49-year-old woman complained of genital bleeding in the follow-up period for her uterine leiomyomas. Close examination disclosed uterine corpus cancer, and biopsy confirmed endometrial carcinoma, grade 1. Total hysterectomy with bilateral salpingo-oophorectomy was performed. Just beneath the uterine serosa, a 9 x 4 mm-sized, yellowish-colored necrotic granuloma containing dead nematode was incidentally observed. Eosinophilic reaction was minimal. The worm was immunoreactive with a monoclonal antibody An-1 against Anisakis simplex antigen. Nested polymerase chain reaction using DNA extracted from formalinfixed, paraffin-embedded sections revealed a gene sequence indicative of Pseudoterranova decipiens. Pseudoterranova, a larval nematode morphologically similar to A. simplex, clinically causes anisakiasis. This is the first case of uterine anisakiasis confirmed morphologically and molecularly.

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