First Report of Bacterial Root Rot, Caused by Dickeya dadantii, on Sweetpotato (Ipomoea batatas) in North Carolina

Necrotic stems and leaves were observed on 2- to 4-month-old, rooted microshoot plants (Vaccinium corymbosum L. ‘Liberty’ and ‘Bluecrop’, V. angustifolium Aiton ‘Putte’, and V. corymbosum × V. angustifolium ‘Polaris’) in a Michigan greenhouse in 2008 and 2009. As the disease progressed, leaves fell off and 80 to 100% of the plants died in some cases. Root rot symptoms were also observed. A fungus was isolated from stem lesions. On potato dextrose agar (PDA), cultures first appeared light tan to orange, then rusty brown and zonate with irregular margins. Chains of orange-brown chlamydospores were abundant in the medium. Macroconidiophores were penicillately branched and had a stipe extension of 220 to 275 × 2.5 μm with a narrowly clavate vesicle, 3 to 4 μm wide at the tip. Conidia were hyaline and cylindrical with rounded ends, (1-)3-septate, 48 to 73 × 5 to 7 (average 60 × 5.5) μm and were held together in parallel clusters. Perithecia were globose to subglobose, yellow, 290 to 320 μm high, and 255 to 295 μm in diameter. Ascospores were hyaline, 2- to 3-septate, guttulate, fusoid with rounded ends, slightly curved, and 30 to 88 × 5 to 7.5 (average 57 × 5.3) μm. On the basis of morphology, the fungus was identified as Calonectria colhounii Peerally (anamorph Cylindrocladium colhounii Peerally) (1,2). The internal transcribed spacer region (ITS1 and ITS2) of the ribosomal DNA and the β-tubulin gene were sequenced (GenBank Accession Nos. HQ909028 and JF826867, respectively) and compared with existing sequences using BLASTn. The ITS sequence shared 99% maximum identity with that of Ca. colhounii CBS 293.79 (GQ280565) from Java, Indonesia, and the β-tubulin sequence shared 97% maximum identity with that of Ca. colhounii CBS 114036 (DQ190560) isolated from leaf spots on Rhododendron sp. in North Carolina. The isolate was submitted to the Centraalbureau voor Schimmelcultures in the Netherlands (CBS 129628). To confirm pathogenicity, 5 ml of a conidial suspension (1 × 105/ml) were applied as a foliar spray or soil drench to four healthy ‘Bluecrop’ plants each in 10-cm plastic pots. Two water-sprayed and two water-drenched plants served as controls. Plants were misted intermittently for 2 days after inoculation. After 7 days at 25 ± 3°C, drench-inoculated plants developed necrotic, sporulating stem lesions at the soil line, while spray-inoculated plants showed reddish brown leaf and stem lesions. At 28 days, three drench-inoculated and one spray-inoculated plant had died, while others showed stem necrosis and wilting. No symptoms were observed on control plants. Fungal colonies reisolated from surface-disinfested symptomatic stem, leaf, and root segments appeared identical to the original isolate. Cy. colhounii was reported to cause a leaf spot on blueberry plants in nurseries in China (3), while Ca. crotalariae (Loos) D.K. Bell & Sobers (= Ca. ilicicola Boedijn & Reitsma) causes stem and root rot of blueberries in North Carolina (4). To our knowledge, this is the first report of Ca. colhounii causing a disease of blueberry in Michigan or the United States. Because of its destructive potential, this pathogen may pose a significant threat in blueberry nurseries. References: (1) P. W. Crous. Taxonomy and Pathology of Cylindrocladium (Calonectria) and Allied Genera. The American Phytopathological Society, St. Paul, MN, 2002. (2) L. Lombard et al. Stud. Mycol. 66:31, 2010. (3) Y. S. Luan et al. Plant Dis. 90:1553, 2006. (4) R. D. Milholland. Phytopathology 64:831, 1974.

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First Report of Phoma terrestris Causing Red Root Rot on Sweet Corn (Zea mays) in North Carolina

Plant Disease ◽

10.1094/pdis-91-8-1054c ◽

2007 ◽

Vol 91 (8) ◽

pp. 1054-1054 ◽

Cited By ~ 5

Author(s):

S. R. Koenning ◽

J. W. Frye ◽

J. K. Pataky ◽

M. Gibbs ◽

D. Cotton

Keyword(s):

North Carolina ◽

Root Rot ◽

Sweet Corn ◽

Adventitious Shoot ◽

Fresh Market ◽

First Report ◽

The North ◽

Field Corn ◽

Foliar Symptoms ◽

Corn Plants

Red root rot, caused by Phoma terrestris E. M. Hansen, caused premature senescence and yield reductions to fresh-market sweet corn in Hyde County, North Carolina in July 2006. Foliar symptoms developed over a period of 5 to 8 days approximately 1 to 2 weeks after anthesis and included desiccation of leaves and poor development of ears. By 3 weeks after pollination, when the sweet corn was harvested, crowns and the first aboveground internode of affected plants were rotted and reddish colored, but roots appeared normal. The root mass of affected plants tended to be greater than that of unaffected plants. Incidence of symptomatic plants was greater than 30% in some fields and was lower on crops planted and harvested early. Symptomatic and asymptomatic plants were adjacent in affected fields. Diseased plants were more common in fields of sweet corn that followed soybean (Glycine max) or a double-crop of onions (Allium cepa) than in fields that followed corn. Incidence of symptomatic plants also differed among adjacent plantings of different sweet corn hybrids. Hybrids ‘173A’, ‘182A’, ‘378a’, and ‘XTH1178’ had a high incidence of symptomatic plants and ‘372A’, ‘278A’, ‘8101’, and ‘8102’ were less affected. Samples of symptomatic plants of the hybrid ‘182A’ were examined at the North Carolina Plant Disease and Insect Clinic during August. Olivaceous black pycnidia with long setae around the ostioles were imbedded in the stalk near the first node aboveground. Numerous conidia (1.8 to 2.3 × 4.5 to 5.5 μm) were released in cirri from pycnidia. When cultured on potato dextrose agar (PDA), the fungus produced a red pigment and intercalary and terminal chlamydospores. Pathogenicity was demonstrated in the greenhouse by transplanting corn seedlings or direct-seeding corn into pots of soil infested with plates of PDA containing chlamydospores and hyphae. A suspension of chlamydospores and hyphae also was injected into the stems of plants 28 days after transplanting. Five replicates of the pathogenicity experiments were repeated twice with noninoculated controls. After 8 weeks, P. terrestris was recovered from the roots of all inoculated plants. Soil inoculation resulted in necrotic root tissue in approximately 25% of inoculated plants. Approximately 90% of inoculated plants had discolored crowns that resembled symptoms from field infected plants. Stem inoculations resulted in necrosis extending 2 to 5 cm from the point of injection and resulted in shoot death of 40% of inoculated plants that resulted in the development of an adventitious shoot. Red root rot was prevalent on field corn in the Delmarva Peninsula throughout the late 1980s and 1990s (1). To our knowledge, this is the first report of this disease causing damage to sweet corn in North Carolina. Foliar symptoms and discoloration of crowns of diseased sweet corn plants were similar to previously described symptoms of red root rot on field corn (2), however, roots of affected sweet corn plants were not substantially rotted and did not have a symptomatic reddish pink or dark carmine color, presumably because sweet corn is harvested prior to the development of root symptoms. References: (1) K. W. Campbell et al. Plant Dis. 75:1186, 1991. (2) D. G. White, ed. Compendium of Corn Diseases. The American Phytopathological Society, St Paul, MN, 1999.

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First Report of Erwinia carotovora subsp. carotovora Causing Bacterial Root Rot of Sweetpotato (Ipomoea batatas) in Louisiana

Plant Disease ◽

10.1094/pdis.1998.82.1.129a ◽

1998 ◽

Vol 82 (1) ◽

pp. 129-129 ◽

Cited By ~ 4

Author(s):

C. A. Clark ◽

M. W. Hoy ◽

J. P. Bond ◽

C. Chen ◽

Y.-K. Goh ◽

...

Keyword(s):

Root Rot ◽

Ipomoea Batatas ◽

Disease Incidence ◽

Erwinia Carotovora ◽

Soft Rot ◽

Stem Rot ◽

Identification System ◽

Natural Occurrence ◽

Storage Roots ◽

First Report

Bacterial root and stem rot of sweetpotato (Ipomoea batatas (L.) Lam.) was first fully characterized in the U.S. in 1977 (2). It was thought to be caused exclusively by Erwinia chrysanthemi. Although a previous report described sweetpotato as a host for E. carotovora subsp. carotovora, based on artificial inoculations, others have reported that neither E. carotovora subsp. carotovora nor E. carotovora subsp. atroseptica decay sweetpotato storage roots (1). In October 1995, storage roots of sweetpotato cv. Beauregard were received from St. Landry Parish, LA, that displayed typical bacterial root rot. Isolations from these roots yielded bacteria that showed a similarity of 0.945 to E. carotovora subsp. carotovora with the Biolog GN Bacterial Identification System (version 3.50). This isolate (Ecc-LH) also differed from isolates of E. chrysanthemi (Ech) from sweetpotato and other hosts in that it was insensitive to erythromycin, did not produce phosphatase or lecithinase, and did not produce gas from glucose. Ecc-LH differed from known strains of E. carotovora subsp. atroseptica in that it did not produce reducing substances from sucrose or acid from palatinose. When Beauregard storage roots were inoculated by inserting micropipette tips containing 50 μl of 1.0 × 108 CFU/ml, both Ecc-LH and Ech-48 produced typical bacterial root rot symptoms. However, when they were compared by infectivity titrations at 28 to 32°C, Ecc-LH was less virulent than Ech-48. Ecc-LH had an ED50 of approximately 1.0 × 106 CFU/ml and did not cause appreciable disease below inoculum concentrations of 1.0 × 105, whereas Ech-48 had an ED50 of approximately 1.0 × 108 and caused soft rot at the lowest concentration tested, 1.0 × 103. Similar disease incidence was observed in infectivity titrations at 22 to 24°C, but Ech-48 caused less severe soft rot. E. carotovora subsp. carotovora was reisolated from inoculated storage roots and its identity was reconfirmed by Biolog. When terminal vine cuttings of Beauregard were dipped in 1.0 × 108 CFU/ml and planted in a greenhouse, bacterial stem rot symptoms developed on plants inoculated with Ech-48 at about 4 weeks postinoculation, or when new growth began. However, no symptoms developed on plants inoculated with Ecc-LH. This is the first report of natural occurrence of E. carotovora subsp. carotovora causing bacterial root rot of sweetpotato in Louisiana. E. chrysanthemi remains the most important pathogen causing bacterial soft rot in sweetpotato since it is widely associated with sweetpotato, is more virulent on storage roots and also causes a stem rot. E. carotovora subsp. carotovora can cause root rot, but has been isolated in only one location to date, is less virulent on storage roots, and apparently does not cause stem rot on the predominant cultivar in U.S. sweetpotato production, Beauregard. References: (1) C. A. Clark and J. W. Moyer. 1988. Compendium of Sweet Potato Diseases. American Phytopathological Society, St. Paul, MN. (2) N. W. Schaad and D. Brenner. Phytopathology 67:302, 1977.

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First Report of Stem and Root Rot of Stevia Caused by Sclerotium rolfsii in North Carolina

Plant Disease ◽

10.1094/pdis-12-13-1238-pdn ◽

2014 ◽

Vol 98 (7) ◽

pp. 1005-1005 ◽

Cited By ~ 4

Author(s):

A. Koehler ◽

H. Shew

Keyword(s):

United States ◽

North Carolina ◽

Root Rot ◽

Mycelial Growth ◽

Sclerotium Rolfsii ◽

Pathogenic Fungi ◽

The United States ◽

Penicillin G ◽

Stem Tissue ◽

First Report

Stevia (Stevia rebaundia) is an emerging crop in the United States. Once established, the crop is grown for 3 to 5 years and is typically harvested twice per growing season. Stevia leaves contain multiple glycosides that are used as a natural noncaloric sweetener that was approved by the USDA in 2008 as a sugar substitute. In commercial plantings of Stevia in North Carolina, wilting and death of plants in first- and second-year plantings were observed in 2012 and 2013. Diseased plants were observed in multiple counties in the state, with first symptoms observed in May of each year and continuing through the summer months. Prior to Stevia, these fields had been planted primarily in a corn-soybean rotation. Symptoms began as moderate to severe wilting of young shoots and chlorosis of leaves, rapidly followed by death of stems and rotting of roots. White mycelial growth was frequently observed at the base of stem tissue. Theses characteristic hyphae of Sclerotium rolfsii were often accompanied by the presence of abundant white to brown sclerotia. Isolations from infected root and stem tissue were made on potato dextrose agar amended with 50 μg/ml of streptomycin sulfate and penicillin G. Isolations from diseased tissue yielded characteristic white hyphae of S. rolfsii (1,3). Numerous sclerotia 0.5 to 2 mm in diameter developed following 4 to 7 days of mycelial growth. Sclerotia were initially white and melanized turning brown with age. To verify pathogenicity, 10-week-old Stevia seedlings were transplanted in 10-cm diameter pots containing sterile 1:1:1 sand, loam, media mix. Inoculum consisted of oat grains infested with one isolate obtained from the field plants. Oats were sterilized on three consecutive days and then inoculated with colonized agar plugs of S. rolfsii. Oats were incubated at room temperature to allow the fungus to thoroughly colonize the oats. Three infested oat grains were added to each test pot and plants were then observed over a 3-week period. Symptoms were observed within 5 days on most plants and included chlorotic leaves, bleached stems, wilting, and necrotic roots. White mycelium and abundant sclerotia were found at the base of plants. Uninoculated plants did not develop any symptoms. This is the first report of S. rolfsii on Stevia in the United States. Kamalakannan et al. (2) reported a root rot disease of Stevia in India and confirmed S. rolfsii as the causal agent. References: (1) R. Aycock. N.C. Agr. Exp. St. Tech. Bull. No. 174, 1966. (2) A. Kamalakannan et al. Plant Pathol. 56:350, 2007. (3) J. E. M. Mordue. Corticium rolfsii. CMI Descriptions of Pathogenic Fungi and Bacteria No. 410. CAB International, Wallingford, UK, 1974.

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First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina

Revista de la Facultad de Ciencias Agrarias UNCuyo ◽

10.48162/rev.39.027 ◽

2021 ◽

Vol 53 (1) ◽

pp. 283-287

Author(s):

Julia A. Martino ◽

Liliana del Valle Di Feo ◽

Mauro Paccioretti ◽

Clara Adriana Contardi ◽

Miguel A. Sanchez ◽

...

Keyword(s):

Sweet Potato ◽

Root Rot ◽

Ipomoea Batatas ◽

Optical Microscope ◽

Thielaviopsis Basicola ◽

Accession Number ◽

Sweet Potatoes ◽

Potential Threat ◽

Black Root Rot ◽

First Report

Symptomatic sweet potato cv Arapey INIA samples were collected from a commercial production field in Colonia Molina, Guaymallén department, Mendoza province, Argentina. They showed dark rounded lesions, sometimes coalescing with white granular mycelium. Fungus was obtained from symptomatic sweet potatoes, which represented the generalized infection that affected the crop. They were seeded in PDA with streptomycin sulfate and incubated for seven days at 21°C, alternating white/black (UV400nm) light. Observations with an optical microscope revealed the presence of hyaline, not septated, cylindrical endoconidia with rounded ends. They were 8-16 μm length and 4–6 μm width. Phialides were 43-46 μm length, rounded bases (7-9 μm width) and tapering to the neck´s tip (4-6 μm width). Brown chlamydospores (aleuriospores), 9-13 μm length and 8-12 μm width, in chains of 2-8 spores were observed. For molecular identification, total genomic DNA was extracted. ITS fragment of 565 pb was amplified using ITS5/ITS4 primers and sequenced. The sequence indicated 99% identity with Berkeleyomyces basicola (synonymous: Thielaviopsis basicola). This was deposited in GenBank as (KX580957) (CBS: C430.74, Gen Bank accession number AF275482.1). This is the first report of B. basicola in sweet potato in Argentina, a potential threat to storage root yields. Highlights: Sweet potato black root rot, new disease in Argentina. First report of Berkeleyomyces basicola causing black root rot on sweet potato in Mendoza, Argentina.

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First Report of Bacterial Stem and Root Rot of Sweetpotato Caused by a Dickeya sp. (Erwinia chrysanthemi) in China

Plant Disease ◽

10.1094/pdis-06-10-0417 ◽

2010 ◽

Vol 94 (12) ◽

pp. 1503-1503 ◽

Cited By ~ 6

Author(s):

L. F. Huang ◽

B. P. Fang ◽

Z. X. Luo ◽

J. Y. Chen ◽

X. J. Zhang ◽

...

Keyword(s):

New York ◽

Root Rot ◽

Type Strain ◽

The United States ◽

Erwinia Chrysanthemi ◽

Dickeya Dadantii ◽

First Report ◽

Guangzhou City ◽

Pcr Products ◽

Production Areas

Since 2006, stem and root rot of sweetpotato has been observed in fields at a number of sweetpotato-production areas in Huidong, Haifeng, Puning, and Zhanjiang counties and Guangzhou City in Guangdong Province of China. Initially, the leaves turn yellow, and a black, water-soaked rot occurs on the bottom of the stems that gradually extends to the top of the stems. Finally, the entire plant collapses and dies. Bacteria were consistently isolated from stems of diseased seedlings by streaking on nutrient agar. Twelve representative isolates were chosen for further characterization. All strains grew at 37°C, were gram negative, facultatively anaerobic, and rod shaped with peritrichous flagella. The strains were negative for oxidase and positive for catalase and tryptophanase (indole production) They fermented glucose, reduced nitrates to nitrites, degraded pectate, produced phosphatase and lecithinase, and utilized citrate, tartrate, malonate, glucose, sucrose, fructose, and maltose, but not trehalose and lactose. These characteristics were similar to those of Erwinia chrysanthemi (Pectobacterium chrysanthemi) (1). PCR was performed on the 16S rDNA gene from isolate H12 (1,503 bp; GenBank Accession No. GU252371) with primers 27f (5′-GAGAGTTTGATCCTGGCTCAG-3′) and reverse primer (5′-GGCTACCTTGTTACGACTTC-3′). Subsequently, PCR products were sequenced. Results of sequence analysis showed the sequence of isolated strain H12 was 99% identical to that of E. chrysanthemi, 99% identical to that of type strain CFBP 1269T of Dickeya dadantii (Accession No. AF520707), and 98% identical to that of type strain CFBP 1200T of D. dianthicola (Accession No. AF520708). Recently, E. chrysanthemi was transferred to Dickeya gen. nov., but it was difficult to identify the species within the genus Dickeya. Seedlings (20 to 30 cm) were planted in 10-cm-diameter plastic pots containing sterilized field soil at room temperature. Four days later, five stem tops of sweetpotato were injected with a bacteria suspension (108 CFU/ml) of approximately 100 μl to fulfill Koch's postulates. Five control plants were inoculated with sterile distilled water. The experiment was conducted three times. All plants were incubated in a chamber at 30°C with high humidity. One to two days after inoculation, symptoms were observed in all inoculated plants and appeared to be identical to those observed in the field. No symptoms were noted on the control plants. The bacterium was reisolated from symptomatic stems of sweetpotato plants. This pathogen was previously reported on sweetpotato in the United States in 1974 (2). To our knowledge, this is the first report of a Dickeya sp. (E. chrysanthemi) causing bacterial stem and root rot of sweetpotato in China. References: (1) D. J. Brenner et al. Page 670 in: Bergey's Manual of Systematic Bacteriology. 2nd ed. Springer, New York, 2005. (2) N. W. Schaad et al. Phytopathology 67:302, 1977.

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First Report of Cylindrocarpon sp. Associated with Root Rot Disease of Strawberry in North Carolina

Plant Disease ◽

10.1094/pdis-01-13-0116-pdn ◽

2013 ◽

Vol 97 (9) ◽

pp. 1251-1251 ◽

Cited By ~ 1

Author(s):

T. B. Adhikari ◽

C. S. Hodges ◽

F. J. Louws

Keyword(s):

North Carolina ◽

Root Rot ◽

Management Strategies ◽

Morphological Characteristics ◽

Internal Transcribed Spacers ◽

Universal Primers ◽

Sodium Hypochlorite Solution ◽

Tissue Samples ◽

First Report ◽

Root Necrosis

Strawberry (Fragaria × ananassa Duchesne) is an economically important fruit crop in North Carolina for domestic consumption and export. In April 2012, outbreaks of a destructive root disease were observed in strawberry cv. Chandler in Buncombe, New Hanover, and Roman counties, North Carolina. Samples from Rowan (ID 13175) and Buncombe (ID 13193) counties submitted to the Plant Disease and Insect Clinic of the Department of Plant Pathology, North Carolina State University, exhibited yellowing and wilting of leaves and extensive root necrosis, and disease severity based on field symptoms ranged from 20 to 30%. To identify the pathogen, five small pieces of necrotic crown and root tissues were taken from each sample, surface disinfested for 1 min in a 1.5% sodium hypochlorite solution, and plated onto potato dextrose agar (PDA) with 0.5 g liter–1 of streptomycin sulfate. Colonies developing from the tissue samples were transferred to PDA. Colonies from both samples were identical, grew relatively slowly, and gradually turned yellowish to partially brownish. After about 7 days, abundant conidia were formed. These were hyaline, mostly straight with both ends rounded, predominantly three septate, and 40 to 50 × 5 to 10 μm. Based on morphological characteristics, these isolates were identified as a species of Cylindrocarpon (1) To confirm the original identification of the fungus as a species of Cylindrocarpon, genomic DNA of both isolates was extracted from mycelia using DNeasy Plant Mini Kit (Qiagen Inc., Valencia, CA) and analyzed using PCR (2). The internal transcribed spacers (ITS)1 and (ITS)2 flanking the 5.8S rRNA regions were amplified and sequenced using universal primers ITS1 (forward) and ITS4 (reverse). The sequences of the 421 bp (GenBank KC847090 and KC847091) of both isolates were identical. Furthermore, a BLASTn search of these sequences showed homology of 99% with the sequences of Cylidrocarpon species (AB369421.1, AM419069.1, AM419074.1, AY295332.1, JN031017.1, JN253505.1, and JQ886422.1), To fulfill Koch's postulates, inoculum of each isolate was prepared and adjusted to 1.5 × 107 conidia/ml using a hemacytometer. ‘Chandler’ strawberry plants were grown in 25-cm diameter plastic pots (one seedling per pot) in the greenhouse and five 6-week-old plants were injected with conidia of each isolate into the base of crown using a 5-ml syringe. The plants were covered with clear plastic for 24 h and left on the greenhouse bench with a 16-h photoperiod and 25/20°C day/night temperatures and assessed for disease development 14 days after inoculation. The inoculated plants exhibited wilting and root necrosis, consistent with the symptoms observed on strawberry plants in the field. Control plants treated with distilled water remained healthy. Isolations were made from the inoculated plants and the fungus used for inoculation was recovered from all plants. The morphology of these isolates was in agreement with published descriptions of Cylindrocarpon (1). To our knowledge, this is the first report of a Cylindrocarpon sp. causing crown and root rot on strawberry in North Carolina and effective disease management strategies need to be explored. References: (1) C. D. Booth. Mycol. Pap. (CMI) 104:1, 1996. (2) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA, 1990.

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First Report of Pythium Root Rot of Stevia Caused by Pythium myriotylum, P. irregulare, and P. aphanidermatum in North Carolina

Plant Disease ◽

10.1094/pdis-10-16-1526-pdn ◽

2017 ◽

Vol 101 (7) ◽

pp. 1331 ◽

Cited By ~ 2

Author(s):

A. M. Koehler ◽

E. C. Lookabaugh ◽

B. B. Shew ◽

H. D. Shew

Keyword(s):

North Carolina ◽

Root Rot ◽

Pythium Myriotylum ◽

First Report ◽

Pythium Root Rot

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Relationships of Preharvest Weather Conditions and Soil Factors to Susceptibility of Sweetpotato to Postharvest Decay Caused by Rhizopus stolonifer and Dickeya dadantii

Plant Disease ◽

10.1094/pdis-11-14-1143-re ◽

2015 ◽

Vol 99 (6) ◽

pp. 848-857 ◽

Cited By ~ 6

Author(s):

Brooke A. Edmunds ◽

Christopher A. Clark ◽

Arthur Q. Villordon ◽

Gerald J. Holmes

Keyword(s):

North Carolina ◽

Soil Moisture ◽

Soil Temperature ◽

Air Temperature ◽

Root Rot ◽

Disease Incidence ◽

Soft Rot ◽

Storage Roots ◽

Dickeya Dadantii ◽

High Soil Temperature

Postharvest soft rots of sweetpotato caused by Rhizopus stolonifer (Rhizopus soft rot) and Dickeya dadantii (bacterial root rot) occur sporadically and can result in significant losses. A 3-year field study related preharvest conditions, including soil texture, chemistry, and fertility; air temperature; soil temperature and moisture; and various cultural practices (153 total variables), to postharvest susceptibility to both diseases in 75 sweetpotato fields in North Carolina and 63 sweetpotato fields in Louisiana. Storage roots were sampled from each field, cured, stored, and inoculated with each pathogen after 100 to 120 days in storage. Disease susceptibility was measured as incidence of diseased storage roots 10 days following inoculation. There was wide variation from field to field in incidence of both diseases (0 to 100% for Rhizopus soft rot and 5 to 95% for bacterial root rot) in both states in each year. Correlations between disease incidence and each of the preharvest variables revealed numerous significant correlations but the variables that correlated with disease incidence were different between North Carolina and Louisiana. Models for both diseases were built by first using forward stepwise regression to identify variables of interest, followed by a mixed-model analysis to produce a final reduced model. For North Carolina fields, postharvest Rhizopus soft rot susceptibility was described by the percentage of the soil cation exchange capacity occupied by calcium, amount of plant-available soil phosphorus, percent soil humic matter, mean air temperature, mean volumetric soil moisture at 40 cm in depth, and mean soil temperature at 2 cm in depth. Postharvest bacterial soft rot susceptibility was described by soil pH and the number of days of high soil temperature late in the season. For Louisiana fields, Rhizopus soft rot susceptibility was described by a complex of variables, including late-season air and soil temperature and late-season days of extreme soil moisture. For bacterial root rot, days of low air temperature and days of high soil temperature late in the season as well as days of low soil moisture best described variation. Although the influence of preharvest variables on postharvest susceptibility was profound for each disease, the complexity of factors involved and differences between the data for the two states makes development of a predictive system extremely difficult.

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