scholarly journals First Report of a New Bacterial Leaf Blight of Rice Caused by Pantoea ananatis and Pantoea stewartii in Benin

Plant Disease ◽  
2017 ◽  
Vol 101 (1) ◽  
pp. 242-242 ◽  
Author(s):  
K. Kini ◽  
R. Agnimonhan ◽  
O. Afolabi ◽  
B. Milan ◽  
B. Soglonou ◽  
...  
Keyword(s):  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Pantoea Ananatis ◽  
First Report ◽  
Pantoea Stewartii

scholarly journals First Report of a New Bacterial Leaf Blight of Rice Caused by Pantoea ananatis and Pantoea stewartii in Togo

Plant Disease ◽  
2017 ◽  
Vol 101 (1) ◽  
pp. 241 ◽  
Author(s):  
K. Kini ◽  
R. Agnimonhan ◽  
O. Afolabi ◽  
B. Soglonou ◽  
D. Silué ◽  
...  
Keyword(s):  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Pantoea Ananatis ◽  
First Report ◽  
Pantoea Stewartii

scholarly journals First Report of New Bacterial Leaf Blight of Rice Caused by Pantoea ananatis in Southeast China

Plant Disease ◽  
2021 ◽  
Author(s):  
Lin Yu ◽  
Changdeng Yang ◽  
Zhijuan Ji ◽  
Yuxiang Zeng ◽  
Yan Liang ◽  
...  
Keyword(s):  
Oryza Sativa L ◽  
Sequence Similarity ◽  
Disease Incidence ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Bacterial Suspension ◽  
Rrna Gene ◽  
Pantoea Ananatis ◽  
Southeast China ◽  
First Report

In autumn 2020, leaf blight was observed on rice (Oryza sativa L., variety Zhongzao39, Yongyou9, Yongyou12, Yongyou15, Yongyou18, Yongyou1540, Zhongzheyou8, Jiafengyou2, Xiangliangyou900 and Jiyou351) in the fields of 17 towns in Zhejiang and Jiangxi Provinces, China. The disease incidence was 45%-60%. Initially, water-soaked, linear, light brown lesions emerged in the upper blades of the leaves, and then spread down to leaf margins, which ultimately caused leaf curling and blight during the booting-harvest stage (Fig. S1). The disease symptoms were assumed to be caused by Xanthomonas oryzae pv. oryzae (Xoo), the pathogen of rice bacterial blight. 63 isolates were obtained from the collected diseased leaves as previously described (Hou et al. 2020). All isolates showed circular, smooth-margined, yellow colonies when cultured on peptone sugar agar (PSA) medium for 24h at 28℃. The cells were all gram-negative and rod-shaped with three to six peritrichous flagella; positive for catalase, indole, glucose fermentation and citrate utilization, while negative for oxidase, alkaline, phenylalanine deaminase, urease, and nitrate reductase reactions. 16S rRNA gene sequence analysis from the 6 isolates (FY43, JH31, JH99, TZ20, TZ39 and TZ68) revealed that the amplified fragments shared 98% similarity with Pantoea ananatis type strain LMG 2665T (GenBank JFZU01) (Table S3). To further verify P. ananatis identity of these isolates, fragments of three housekeeping genes including gyrB, leuS and rpoB from the 6 isolates were amplified and sequenced, which showed highest homology to LMG 2665T with a sequence similarity of 95%-100% (Table S3). Primers (Brady et al. 2008) and GenBank accession numbers of gene sequences from the 6 isolates are listed in Table S1 and Table S2. Phylogenetic analysis of gyrB, leuS and rpoB concatenated sequences indicated that the 6 isolates were clustered in a stable branch with P. ananatis (Fig. S2). Based on the above morphological, physiological, biochemical and molecular data, the isolates are identified as P. ananatis. For pathogenicity tests, bacterial suspension at 108 CFU/mL was inoculated into flag leaves of rice (cv. Zhongzao39) at the late booting stage using clipping method. Water was used as a negative control. The clipped leaves displayed water-soaked lesions at 3 to 5 days after inoculation (DAI); then the lesion spread downward and turned light brown. At about 14 DAI, blight was shown with similar symptoms to those samples collected from the rice field of Zhejiang and Jiangxi provinces (Fig. S1). In contrast, the control plants remained healthy and symptomless. The same P. ananatis was re-isolated in the inoculated rice plants, fulfilling Koch’s postulates. In the past decade, P. ananatis has been reported to cause grain discoloration in Hangzhou, China (Yan et al. 2010) and induce leaf blight as a companion of Enterobacter asburiae in Sichuan province, China (Xue et al. 2020). Nevertheless, to the best of our knowledge, this is the first report of P. ananatis as the causative agent of rice leaf blight in southeast China. This study raises the alarm that the emerging rice bacterial leaf blight in southeast China might be caused by a new pathogen P. ananatis, instead of Xoo as traditionally assumed. Further, the differences of occurrence, spread and control between two rice bacterial leaf blight diseases caused by P. ananatis and Xoo, respectively need to be determined in the future.

scholarly journals First Report of a Leaf Blight in Rice (Oryza sativa) Caused by Pantoea ananatis and Pantoea stewartii in Thailand

Plant Disease ◽  
2020 ◽  
Vol 104 (2) ◽  
pp. 562
Author(s):  
N. Arayaskul ◽  
S. Poompouang ◽  
P. Lithanatudom ◽  
S. K. Lithanatudom
Keyword(s):  
Oryza Sativa ◽  
Leaf Blight ◽  
Pantoea Ananatis ◽  
First Report ◽  
Pantoea Stewartii

scholarly journals First Report of Bacterial Leaf Blight of Strawberry Caused by Pantoea ananatis in Nova Scotia, Canada

Plant Disease ◽  
2020 ◽  
Vol 104 (1) ◽  
pp. 276-276
Author(s):  
Sruti Bajpai ◽  
Pushp Sheel Shukla ◽  
Mohd Adil ◽  
Samuel Asiedu ◽  
Kris Pruski ◽  
...  
Keyword(s):  
Nova Scotia ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Pantoea Ananatis ◽  
First Report

scholarly journals First Report of a New Bacterial Leaf Blight of Chinese Cabbage (Brassica rapa pekinensis) Caused by Pantoea ananatis in China

Plant Disease ◽  
2019 ◽  
Vol 103 (11) ◽  
pp. 2942
Author(s):  
Ye Zhang ◽  
Lei Zheng ◽  
Hanzhong Gao ◽  
Qingqing Song ◽  
Jianwei Gao
Keyword(s):  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Pantoea Ananatis ◽  
First Report

First Report of Pantoea stewartii subsp. indologenes Causing Leaf Blight on Rice in Malaysia

Plant Disease ◽  
2019 ◽  
Vol 103 (6) ◽  
pp. 1407-1407 ◽  
Author(s):  
M. M. F. Azizi ◽  
S. I. Ismail ◽  
E. M. Hata ◽  
D. Zulperi ◽  
M. Y. Ina-Salwany ◽  
...  
Keyword(s):  
Leaf Blight ◽  
First Report ◽  
Pantoea Stewartii

scholarly journals First Report of Bacterial Leaf Blight of Carrot Caused by Xanthomonas hortorum pv. carotae in Korea

Plant Disease ◽  
2014 ◽  
Vol 98 (2) ◽  
pp. 275-275 ◽  
Author(s):  
I.-S. Myung ◽  
M.-J. Yoon ◽  
J.-Y. Lee ◽  
G.-D. Kim ◽  
M.-H. Lee ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Negative Control ◽  
Leaf Blight ◽  
Gyrb Gene ◽  
Bacterial Leaf Blight ◽  
Distilled Water ◽  
First Report ◽  
Rpod Gene ◽  
Phenotypic Tests ◽  
Hydrolysis Of

In December 2012, symptoms of typical bacterial leaf blight were observed on carrot plants (Daucus carota L. subsp. sativus) cultivated in commercial fields in Kujwa, Jeju, Korea. The disease was detected in 40% of 50 fields surveyed with an incidence of 10% on average. The bacterial leaf blight lesions on leaf blades were elongated, dark brown to black with water-soaked edges and chlorotic halos. Lesions were also crescent-shaped to V-shaped on leaflets. Four bacterial isolates were recovered on trypticase soy agar from leaf lesions that were surface-sterilized in 70% ethyl alcohol for 20 s. Identity of the isolates was confirmed by PCR product (1,266-bp) using a specific primer set for Xanthomonas hortorum pv. carotae (Kendrick 1934) Vauterin et al. 1995, XhcPP03 (1). All isolates were gram-negative, aerobic rods with a single polar flagellum. Isolates were positive for catalase and negative for oxidase. In phenotypic tests for differentiation of Xanthomonas (2), the isolates positive for mucoid growth on yeast extract-dextrose-calcium carbonate agar, growth at 35°C, hydrolysis of esculin, protein digestion, alkaline in litmus milk, acid production from arabitol, and utilization of glycerol and melibiose. The isolates were negative for growth on SX medium, hydrolysis of starch, and ice nucleation. The gyrB gene (863 bp) and the rpoD gene (870 bp) were sequenced to aid identification of the original isolates using published PCR primer sets, Xgyr1BF/Xgyr1BR and XrpoD1F/XrpoD1R (4), respectively. Sequences of the gyrB gene (GenBank accessions KC920729 to KC920732) from the carrot isolates shared 100% sequence identity with that of the X. hortorum pv. carotae strain NCPPB 425 (EU285243). In phylogenetic analyses based on the partial sequences of the gyrB and the rpoD genes for Xanthomonas spp. available at NCBI (4), and sequences of the carrot isolates (KC920734 to KC920737 for rpoD gene) using the Neighbor-joining method in MEGA Version 5.1 (3), the isolates were clustered in the X. hortorum-cynarae-garnderi group. Pathogenicity of the isolates was tested by spray inoculation with a bacterial suspension (106 CFU/ml) prepared in sterile distilled water at 6 to 7 true-leaf stage (three plants per isolate). Sterile distilled water was used as negative control. The inoculated plants were incubated in a growth chamber (25°C and 95% relative humidity [RH]) for 15 hr, and then transferred to a greenhouse at 24 to 28°C and 65% RH. Characteristic leaf blight symptoms developed on inoculated carrot plants, while no symptoms were observed on the negative control plants 14 days after inoculation. The bacterium was re-isolated from symptomatic tissue and the identity confirmed through gyrB gene sequence analysis (4). Based on PCR, morphological and phenotypic tests, sequence analysis, and pathogenicity assays, the isolates were identified as X. hortorum pv. carotae. To our knowledge, this is the first report of bacterial leaf blight of carrot caused by X. hortorum pv. carotae in Korea. The detection of this pathogen could have a significant economic impact due to yield losses from disease development. Consolidation of quarantine inspection on imported carrot seeds needs to control an outbreak of the disease. Crop rotation and plowing are recommended to reduce incidence of the disease in the infested fields. References: (1) J. A. Kimbrel et al. Mol. Plant Pathol. 12:580, 2011. (2) N. W. Schaad et al. Page 189 in: Laboratory Guide for Identification of Plant Pathogenic Bacteria. 3rd ed. N. W. Schaad et al., eds. The American Phytopathological Society, St. Paul, MN, 2001. (3) K. Tamura et al. Mol. Biol. Evol. 28:2731, 2011. (4) J. M. Young et al. Syst. Appl. Microbiol. 31:366, 2008.

scholarly journals HOST RANGE PANTOEA ANANATIS THE CAUSAL AGENT OF BACTERIAL LEAF BLIGHT ON ALLIUM SPP.

2019 ◽  
Vol 6 (1) ◽  
pp. 27
Author(s):  
Asrul Asrul ◽  
Umrah Umrah
Keyword(s):  
Host Range ◽  
Pathogenic Bacteria ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Pantoea Ananatis ◽  
Randomized Design ◽  
Spring Onion ◽  
Completely Randomized Design ◽  
Blight Disease ◽  
Range Test

Bacterial leaf blight disease caused by Pantoea ananatis has been reported affects some species of Allium spp. Here, we determined the species of Allium spp. that are not the hosts of the P.ananatis through a host range test. The study designed as a completely randomized design with five replications. Pathogenic bacteria of P. ananatis inoculated to shallot, onion, spring onion, garlic, chives, leek, and cung spring onion in the greenhouse of the Faculty of Agriculture Tadulako University. The incubation period and symptoms of after inoculated by P. ananatis then observed. The results showed that four llium species (onion, onion, scallion, and garlic) were hosts of                P. ananatis while the other three species (chives, leek, and cung spring onion) were not hosts of          P. ananatis.

scholarly journals First Report of Xanthomonas phaseoli pv. dieffenbachiae Causing Bacterial Leaf Blight on Anthurium andreanum in Mexico

Plant Disease ◽  
2020 ◽  
Vol 104 (4) ◽  
pp. 1252-1252
Author(s):  
L. Flores-López ◽  
O. Morales-Galván ◽  
A. Cando-Narváez ◽  
J. Barreto-Turiján ◽  
B. Xoconostle-Cázares ◽  
...  
Keyword(s):  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
First Report ◽  
Anthurium Andreanum
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