scholarly journals First report of the parasitic invasive weed field dodder (Cuscuta campestris) parasitizing the confamilial invasive weed common morning-glory (Ipomoea purpurea) in Shandong, China

Plant Disease ◽  
2020 ◽  
Author(s):  
Xiao-Jian Qu ◽  
Shou-Jin Fan
Keyword(s):  
South America ◽  
Morphological Characteristics ◽  
Shandong Province ◽  
Morning Glory ◽  
Molecular Evidence ◽  
Accession Number ◽  
Invasive Weed ◽  
First Report ◽  
Ipomoea Purpurea ◽  
Cuscuta Campestris

Common morning-glory (Ipomoea purpurea (L.) Roth, Convolvulaceae), an annual herbaceous vine native to South America, was first recorded to be cultivated in China in 1890, and since then it has invaded all provinces of China. It was one of the 18 alien invasive species in China (MEE. 2014). As an invasive weed, it can readily invade dry lands, orchards, and nurseries and compete for sunlight by wrapping other plants. On 20 September 2019 and 18 July 2020, I. purpurea was found to be parasitized by a dodder species (also Convolvulaceae) in Lushan Mountain (36°21′N, 118°3′E, 569 m elevation), Shandong province, China (Fig. S1). Within and area of ca. 100 m2, dozens of individuals of common morning-glory were parasitized by the leafless stems of dodder. After removal of the haustrial connection of the dodder stem from the I. purpurea stem, brownish black lesions around uneven holes were visible on the I. purpurea stem, with broken haustoria clearly visible to our naked eye remaining in the I. purpurea stem (Fig. S1). Anatomical results showed that the haustoria of dodder penetrate I. purpurea stem and xylem elements connect the vascular systems of both the parasitic and host plant (Fig. S1). Based on morphological characteristics of stems, inflorescences, calyx, corolla, stamens, and capsules as described in Costea et al. (2006), this dodder was identified as Cuscuta campestris Yunck. (i.e., field dodder). Field dodder is readily distinguished from C. chinensis and C. australis in China by the capsules with persistent corollas enveloping 1/3 or less of its base and the spreading and inflexed corolla lobes with acute to acuminate apices. In order to further confirm the identity of the species, total genomic DNA was extracted and sequenced using genome-skimming method as described in Qu et al. (2019). An 831-bp region of 18S-ITS1-5.8S-ITS2-26S for the dodder studied was assembled, examined, and deposited in GenBank under accession number MN718805. The new sequence has 100% similarity with other available sequences of C. campestris (accession number: KT383104, KT383150, KY968857). Phylogenetic analysis also placed the new dodder accession with other accessions of C. campestris (Fig. S2a). In addition, the plastome sequence of the dodder studied was assembled (86,727 bp in length) and deposited in GenBank under accession number MN708214, and a BLAST analysis found that it was 99.98% similar to that of C. gronovii (accession number: AM711639). The plastome of C. gronovii was published by Funk et al. (2007). However, Costea et al. (2015) indicated that Funk et al. (2007) misidentified C. campestris as C. gronovii. Furthermore, our phylogenetic tree strongly supported the identification of the dodder studied as C. campestris (Fig. S2b). Therefore, the dodder on common morning-glory in Shandong province was finally identified as C. campestris according to morphological and molecular evidence. The specimen of C. campestris on I. purpurea was deposited at the herbarium of the College of Life Sciences, Shandong Normal University (voucher number: 092012B). Field dodder, the second most common dodder species in North America, is the most widespread Cuscuta weed in the world and has been found in Africa, Asia, Australia, Europe, and South America (Holm et al. 1997). To our knowledge, this is the first report of the parasitic invasive weed C. campestris parasitizing the invasive weed I. purpurea in Shandong of China. This is also the first report of Cuscuta species parasitizing confamilial Ipomoea species, which is especially noteworthy given that the genus Cuscuta is sister to the genus Ipomoea. This study provides a good model for exploring gene flow between species of closely related genera with different lifestyle. Another implication of this study is that customs and departments of inspection and quarantine need to quarantine the seeds or plants of both dodders and common morning-glories.

scholarly journals First report of Pythium aphanidermatum causing root rot of head lettuce in China

Plant Disease ◽  
2021 ◽  
Author(s):  
Jun-shan Qi ◽  
Bo Zhang ◽  
Li-guo Ma ◽  
Guoping Ma ◽  
Shu-jun Qin ◽  
...  
Keyword(s):  
Lactuca Sativa ◽  
Root Rot ◽  
Morphological Characteristics ◽  
Its Region ◽  
Pythium Aphanidermatum ◽  
Coi Gene ◽  
Accession Number ◽  
Calcium Hypochlorite ◽  
First Report ◽  
Corn Kernels

Head lettuce (Lactuca sativa L.) is an important crop for fresh consumption in China. In Shandong Province, head lettuce is planted in spring and in autumn each year. Because of the on-and-off rain for three weeks, head lettuce plants planted directly into the field in Jiyang City, in July 2017, 20% of the plants rapidly showed symptoms of rotting, water-soaked lesions on roots and stem bases, and then death. The diseased plants first appeared in low-lying areas prone to water accumulation. One-millimeter pieces were excised from water-soaked roots and stem bases, dipped in a 0.2% calcium hypochlorite solution for 10 min, then placed on V8 medium, and incubated in the dark at 28°C for 5 d. Two Pythium-like strains were isolated from the roots and stems. The isolates transferred to CMA and grown for 7 d, and the morphological characteristics of the two isolates on corn meal agar (CMA) were white with dense, cottony, aerial and well-branched mycelia. The two isolates produced sporangia, oogonia, antheridia and oospores. Most of the sporangia were lobate. The oogonia were smooth, nearly globose and terminal. Oospores were globose, smooth and aplerotic. The average dimensions of 50 oogonia and oospores respectively ranged from 19.5 to 25.2 (av. 23.1) µm and 17.8 to 22.3 (av. 19.9) µm. The antheridia were broadly sac-shaped. The isolates morphological characteristics were consistent with P. aphanidermatum (van der Plaats-Niterink, 1981). The COI gene and ITS region of the rDNA were amplified and sequenced using primers FM55/FM52R (Long et al. 2012) and ITS1/ITS4 (White et al. 1990), respectively. The two aligned COI sequences were identical for both isolates, as were the two ITS sequences. BLASTn analysis of the 1,133-bp COI sequence (accession no. MT952703) resulted in a 100% identity with accession number AY129164 from Lactuca sativa, which belongs to P. aphanidermatum, and the 808-bp ITS sequence (accession no. MT921597) showed a 99% identity with Genbank accession number HQ643442 belonging to P. aphanidermatum. Koch’s postulates were conducted by first soaking corn kernels for 24 h in water, and then autoclaving for 2 h at 121˚C. Isolate SDHL-1 was grown on CMA for 10 days, after which agar plugs were transferred to the sterilized corn kernels and incubated at 28℃ for approximately 15 d, until the corn kernels were covered in white hyphae. Ten healthy head lettuce plants were transplanted into a sterilized loam potting soil artificially infested with the corn inoculum (3 g inoculum per 100 g loam mixture). Inoculated plants and noninoculated controls were maintained in a greenhouse at 28°C and 100% relative humidity with a 12-h photoperiod; the experiment was repeated once. All twenty inoculated plants exhibited symptoms within one week similar to those observed. Pythium aphanidermatum was recovered only from the water-soaked roots and stem bases of inoculated plants and the re-isolated cultures again identified based on morphological characteristics and sequencing of the ITS and COI genes. No symptoms were observed on the control plants. Sclerotinia sclerotiorum is reported to cause stem base rot of L. sativa in China (Zhou et al. 2011). To our knowledge, however, this is the first report of root rot of head lettuce caused by Pythium aphanidermatum. Identification of the pathogen will assist in devising strategies to reduce yield loss.

scholarly journals First Report of Sweet potato leaf curl Georgia virus Infecting Tall Morning Glory (Ipomoea purpurea) in China

Plant Disease ◽  
2014 ◽  
Vol 98 (11) ◽  
pp. 1588-1588
Author(s):  
S. B. Zhang ◽  
Z. G. Du ◽  
Z. Wang ◽  
Y. F. Tang ◽  
X. M. She ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Rolling Circle Amplification ◽  
The United States ◽  
Morning Glory ◽  
Central China ◽  
Potato Leaf ◽  
First Report ◽  
Ipomoea Purpurea ◽  
Sequence Identity ◽  
Leaf Curl

In September 2013, tall morning glory (Ipomoea purpurea) plants showing vein yellowing and leaf curl symptoms typical of a begomovirus infection were observed in Jingzhou, Hubei Province, China. Total nucleic acids were extracted from a symptomatic plant using cetyltrimethylammonium bromide (CTAB). Rolling circle amplification (RCA) was conducted using TempliPhi kit (GE Healthcare) to recover the genome of a putative begomovirus. Digestion of the RCA product with PstI yielded a ~2.8 kbp DNA fragment suggestive of a monomerized begomoviral genome. The fragment was cloned and sequenced and the sequence was deposited in GenBank under accession no. KF769447. SDTv1.0 (species demarcation tool) analysis revealed that the putative begomovirus showed 98.5 and 92.0% nucleotide sequence identity with Sweet potato leaf curl Georgia virus (SPLCGV)-[China:Hebei:2011] (GenBank Accession No. JX448368) and SPLCGV-[US:Geo:16] (AF326775), respectively. The virus contained six ORFs, which encoded proteins showing 96.5 to 100% and 90.6 to 95.6% amino acid sequence identity with their counterparts of SPLCGV-[China:Hebei:2011] and SPLCGV-[US:Geo:16], respectively. Thus, the virus should be considered as an isolate of SPLCGV-[China:Hebei:2011]. Tall glory morning in a nearby field (which covers an area of 3 square kilometers) was surveyed and 70 to 100% of plants were found showing symptoms reminiscent of begomoviral infection. Total nucleic acid was extracted from 13 randomly selected (10 symptomatic and 3 healthy) plants and used as templates for PCR with a pair of specific primers (5′-CGCAGCCTTTCCACACTATC-3′/5′-AAAACAGTTTGGGCTCGGTC-3′) designed according to the sequence described above. Positive results were obtained for all of the symptomatic, but none of the healthy-looking tall morning glory plants. SPLCGV (genus Begomovirus, family Geminiviridae) was reported to infect sweet potato (I. batatas) in the United States (4), India (2), and China (3). To our knowledge, this is the first report of SPLCGV infecting tall morning glory in China. Also, it is the first report of a geminivirus in Hubei, a province of central China. Whereas the finding of SPLCGV in sweet potato (3) may be a result of vegetative propagation of this crop, the detection of SPLCGV in tall morning glory, an annual plant, raises the possibility that this virus is transmissible and is spreading in China. References: (1) B. Muhire et al. Arch. Virol. 158:1411, 2013. (2) G. Prasanth and V. Hegde. Plant Dis. 92:311, 2008. (3) Y. Qin et al. Plant Dis. 97:1388, 2013. (4) R. A. Valverde and D. L. Gutierrez. Rev. Mex. Fitopatol. 21:128, 2003.

scholarly journals First Report of New Bacterial Leaf streak of Rice Caused by Pantoea ananatis in China

Plant Disease ◽  
2022 ◽  
Author(s):  
Xinhua Ding ◽  
Chongchong Lu ◽  
Mingxia Hao ◽  
Lingguang Kong ◽  
Lulu Wang ◽  
...  
Keyword(s):  
16S Rdna ◽  
Shandong Province ◽  
Bacterial Suspension ◽  
Distilled Water ◽  
Bacterial Leaf Streak ◽  
Accession Number ◽  
Pantoea Ananatis ◽  
First Report ◽  
Rice Leaves ◽  
Dark Green

Rice (Oryza sativa L.) is the largest grain crop, accounting for about 40 % of the total grain production in China. In mid-July 2021, bacterial leaf streak-like disease emerged in rice varieties Chunyou584 and Yongyou2604 in Linyi city, Shandong Province, China. Disease incidences of the disease ranged from 80% to 90% in the surveyed fields. Infected rice leaves displayed dark green to yellowish-brown water-soaked thin streaks, and a large amount of beaded yellow oozes were observed on the lesions. After drying, there were gelatinous granules that were not easy to fall off and spread between leaf veins (Fig.S1A). According to the field symptoms of this disease, it was preliminarily suspected to be rice bacterial leaf streak caused by Xanthomonas oryzae pv. oryzicola (Xoc), which is a guaranteed disease in China. To isolate the causal agent, leaf discs (~1 cm2) of diseased leaves were collected from the margins of the lesions, surface sterilized and ground into pieces in sterile double distilled water. The 10-3, 10-4 and 10-5 dilutions were spread onto peptone sugar agar (PSA) and incubated at 28°C for 36 hours. Yellow mucous bacterial colonies were consistently obtained on PSA medium. To identify the pathogen, fragments of the 16S rDNA, leuS and rpoB were amplified and sequenced using the primers previously reported (Yu et al. 2021). Three strains (LY01, LY02 and LY03) showed identical colony morphology and LY01 was used for further analyses. Sequence analyses showed that the fragments of 16S rDNA (955 bp, GenBank accession number: OK261898), leuS (755 bp, GenBank accession number: OK298387) and rpoB (926 bp, GenBank accession number: OK298388) of strain LY01 shared 99.16%, 99.46% and 100% similarities with those of Pantoea ananatis TZ39 (GenBank accession numbers: CP081342.1 for 16S rDNA, MW981338.1 for leuS and MW981344.1 for rpoB), respectively, which suggest the pathogenic bacterial strain LY01 isolated is P. ananatis. In addition, the single colony of P. ananatis LY01 was shown as Fig. S2B. Furthermore, pathogenicity tests were also performed according to the following steps. Bacterial suspension at OD600=0.1 was inoculated into eight rice leaves of four healthy rice plants (Chunyou 584) at 25-33°C and 60%-80% relative humidity in the field using a clipping method (Yang et al. 2020) or spraying methods, and sterile distilled water was as negative control. The clipped leaves (Fig. S1B) and spray-inoculated leaves (Fig. S1C) showed dark green water-soaked streaks at 14 days after inoculation, respectively, which showed similar symptoms with those samples collected from the fields (Fig. S1A). On contrary, the control rice leaves remained healthy and symptomless (Fig. S2A). The bacterium was re-isolated in the inoculated rice leaves and the re-isolated bacterial isolates, which was confirmed by sequencing 16S rDNA, leuS and rpoB, incited the same symptoms as in fields, which fulfills Koch’s postulates. In the past decade, P. ananatis was reported to result in grain discoloration and leaf blight in China (Yan et al. 2010; Xue et al. 2020, Yu et al. 2021), which could result in 40% - 60% yield losses. To our best knowledge, this is the first report of the bacterial leaf streak-likely disease occurred in Shandong Province caused by P. ananatis, so we named it as Pantoea leaf streak of rice. Although P. ananatis was also reported in Zhejiang province and Jiangxi province, which caused leaf streak lesions on rice, the disease symptoms are completely different from those of Pantoea leaf streak of rice. To the best of our knowledge, this is the first report of Pantoea leaf streak of rice caused by P. ananatis. This study provides sloid evidence that Pantoea leaf streak of rice in Eastern China can be caused by the new pathogen, P. ananatis, rather than Xoc as traditionally assumed. Disease development and quarantine of the new Pantoea leaf streak of rice disease caused by P. ananatis on rice need more attention in the near future.

scholarly journals First Report of Blight on Ipomoea purpurea Caused by Phytophthora ipomoeae

Plant Disease ◽  
2004 ◽  
Vol 88 (11) ◽  
pp. 1283-1283 ◽  
Author(s):  
G. Badillo-Ponce ◽  
S. P. Fernández-Pavía ◽  
N. J. Grünwald ◽  
E. Garay-Serrano ◽  
G. Rodríguez-Alvarado ◽  
...  
Keyword(s):  
Morphological Characteristics ◽  
Moist Chamber ◽  
First Report ◽  
Ipomoea Purpurea ◽  
Genotypic Analysis ◽  
Detached Leaves ◽  
Pathogenicity Tests ◽  
Symptomatic Tissue ◽  
Short Pedicel ◽  
Allozyme Genotype

Several wild species of Ipomoea grow in the central highlands of Mexico. During the summer of 1999, in Metepec, Mexico, blighted leaves and petioles of Ipomoea purpurea were collected from diseased plants and placed in a moist chamber to induce sporulation. Sporangia that formed on the lesions were transferred with a piece of agar to selective rye agar medium (2). Phytophthora ipomoeae was consistently isolated. Species identification was based on sporangial and gametangial characteristics of five cultures grown on rye agar. Sporangia were mainly ellipsoid but occasionally ovoid, semipapillated, and deciduous with a short pedicel. All isolates were homothallic with smooth-walled and aplerotic oospores. Genotypic analysis for the allozymes Peptidase (Pep) and Glucose-6-phosphate isomerase (Gpi) indicated that all five isolates belonged to one genotype with alleles 78/78 (Pep) and 108/108 (Gpi). Morphological characteristics and the allozyme genotype correspond to the new, recently described species P. ipomoeae Flier & Grünwald (1) isolated from I. orizabensis (Pelletan) Ledeb. ex Steud. (I. tyrianthina) Lindl. and I. longepedunculata (Mart. & Gal.) Hemsl. Pathogenicity tests were carried out with leaves from greenhouse-grown I. purpurea plants. Detached leaves were inoculated with a suspension of 103 sporangia per ml and kept in a moist chamber at room temperature (17 ± 3°C). Lesions were observed between 7 and 15 days after inoculation and were characteristic of those observed in the field. The pathogen was reisolated from inoculated symptomatic tissue. To our knowledge, this is the first report of blight on I. purpurea caused by P. ipomoeae. References: (1) W. Flier et al. Mycol. Res. 106:848, 2002. (2) N. J. Grünwald et al. Phytopathology 91:882, 2001.

scholarly journals First Report of Rust Disease Caused by Uromyces galegae on Galega officinalis in Turkey

Plant Disease ◽  
2006 ◽  
Vol 90 (4) ◽  
pp. 525-525 ◽  
Author(s):  
B. Tunali ◽  
A. Yildirim ◽  
M. C. Aime ◽  
J. R. Hernández
Keyword(s):  
Morphological Characteristics ◽  
The United States ◽  
Herbarium Specimens ◽  
Rust Disease ◽  
Invasive Weed ◽  
First Report ◽  
Galega Officinalis ◽  
Western Asia ◽  
On Line ◽  
The U.S

Galega officinalis L. is an obnoxious invasive weed in the United States and a potential target for biological control efforts. The plant, a member of the legume family, is native to western Asia and southern Europe. During September 2001, uredinial pustules were observed on leaves of G. officinalis L. in Kizilcahamam, Ankara. Specimens were examined microscopically and compared with published descriptions (2) and herbarium specimens in the U.S. National Fungus Collections, Beltsville, MD. The fungus was subsequently identified as Uromyces galegae (Opiz) Sacc. on the basis of morphological characteristics of the uredinia, urediniospores, and teliospores. The following description is from the Turkish material: uredinia subcircular to oblong, hypophyllous, rarely epiphyllous at petiole, and 0.5 to 1 mm in diameter; urediniospores subovoid to subglobose, 17.5 to 20.0 × 19.5 to 22.5 μm (average = 18.0 × 20.0 μm), wall 1 to 2 μm thick, finely echinulate, cinnamon brown, and with 3 to 5 usually equatorial germ pores; telia similar to uredinia; teliospores irregular in shape ranging from globose to ovoid to triangular, apex papillate, wall 2 to 3 μm thick, thicker at the apex, chestnut brown, strongly verrucose to tuberculate, 17.5 to 22.5 × 22.5 to 27.5 μm (average = 20.3 × 24.5 μm), pedicel hyaline, and easily broken. Voucher specimens are deposited in the U.S. National Fungus Collections (BPI 863535); a nucleotide sequence spanning the ITS2 and 28S rDNA genes of this isolate was obtained and deposited in Gen-Bank (Accession No. DQ250133). U. galegae has been reported on G. officinalis from Bulgaria, Greece, and Italy (1). To our knowledge, this is the first report of U. galegae in Turkey and marks the eastern-most record for its distribution. References: (1) D. F. Farr et al. Fungal Databases. Systematic Botany and Mycology Laboratory, On-line publication, USDA-ARS, 2005. (2) M. Pantidou and D. M. Henderson. Notes R. Bot. Gard. Edinb. 29:277, 1969.

scholarly journals First Report of Powdery Mildew Caused by Golovinomyces asterum var. solidaginis on Invasive Weed Solidago gigantea in Korea

Plant Disease ◽  
2013 ◽  
Vol 97 (8) ◽  
pp. 1120-1120 ◽  
Author(s):  
S. E. Cho ◽  
J. H. Park ◽  
S. H. Hong ◽  
H. D. Shin
Keyword(s):  
North America ◽  
Powdery Mildew ◽  
Morphological Characteristics ◽  
Width Ratio ◽  
Limiting Factor ◽  
Indigenous Plants ◽  
Invasive Weed ◽  
First Report ◽  
Solidago Gigantea ◽  
Powdery Mildews

Solidago gigantea Aiton (syn. S. serotina Aiton), known as giant goldenrod, is native to North America and has been invasive in Europe and Asia. In Korea, this plant was accidentally introduced around the 1960s and has become widely naturalized by replacing indigenous plants and disrupting the native ecosystem (3). In October 2012, hundreds of giant goldenrod plants growing wild in riverine areas and roadsides were found affected by a powdery mildew in Busan, Korea. Voucher specimens were deposited in the Korea University Herbarium (KUS). Symptoms first appeared as circular to irregular white patches, which subsequently showed abundant hyphal growth on both sides of the leaves. Appressoria on the mycelium were nipple-shaped to moderately lobed. Conidiophores measured 120 to 240 × 10 to 12.5 μm, were arising laterally from hyphal mother cells, and produced 2 to 6 immature conidia in chains with a sinuate outline, followed by 2 to 3 cells. Foot-cells in conidiophores were 42 to 70 μm long and characterized by a distinctly curved base. Conidia were hyaline, ellipsoid to ovate, measured 28 to 42 × 17 to 24 μm (length/width ratio = 1.4 to 2.1), contained small oil drops, lacked distinct fibrosin bodies, and produced germ tubes on the subterminal position. No chasmothecia were observed. The morphological characteristics described above were typical of the Euoidium type anamorph of the genus Golovinomyces, and the fungus measurements and structures were consistent with those of G. asterum var. solidaginis U. Braun (1). To confirm the identity of the causal fungus, the complete ITS region of rDNA from isolate KUS-F27219 was amplified with primers ITS5 and P3 (4) and sequenced. The resulting 508-bp sequence was deposited in GenBank (Accession No. KC513763). A GenBank BLAST search of this sequence revealed >99% similarity with the ITS sequences of G. cichoracearum from Australia (GQ183940 ex Solidago sp.) and Japan (AB077625 ex S. altissima L. and AB077627 ex S. virgaurea subsp. asiatica Kitam. ex Hara). The G. cichoracearum isolates on Solidago spp. listed above are now placed in G. asterum var. solidaginis (1). Pathogenicity was confirmed through inoculation by gently pressing diseased leaves onto leaves of five healthy potted giant goldenrods. Five non-inoculated plants served as controls. Plants were maintained in a greenhouse at 24 to 30°C. Inoculated plants developed signs and symptoms after 7 days, whereas the control plants remained symptomless. The fungus present on inoculated plants was morphologically identical to that originally observed, fulfilling Koch's postulates. Association of S. gigantea with Golovinomyces powdery mildews has been known in North America, South America, Europe, New Zealand, Central Asia (Iran, Kazakhstan, and Kyrgyzstan), and Japan (2). To our knowledge, this is the first report of powdery mildew caused by G. asterum var. solidaginis on S. gigantea in Korea. Our field observations suggest that the powdery mildew could be a limiting factor to suppress the expansion of this invasive weed in Korea. References: (1) U. Braun and R. T. A. Cook. Taxonomic Manual of the Erysiphales (Powdery Mildews), CBS Biodiversity Series No.11. CBS, Utrecht, 2012. (2) D. F. Farr and A. Y. Rossman. Fungal Databases. Syst. Mycol. Microbiol. Lab., Online publication, ARS, USDA, retrieved January 22, 2013. (3) S. M. Oh et al. Kor. J. Weed Sci. 22:280, 2002. (4) S. Takamatsu et al. Mycol. Res. 113:117, 2009.

scholarly journals First Report of Lasiodiplodia pseudotheobromae Causing Collar Rot of Peanut in Shandong Province, China

Plant Disease ◽  
2021 ◽  
Author(s):  
Xia Zhang ◽  
Ying Li ◽  
Manlin Xu ◽  
Zhiqing Guo ◽  
Jing Yu ◽  
...  
Keyword(s):  
Morphological Characteristics ◽  
Shandong Province ◽  
Beta Tubulin ◽  
Collar Rot ◽  
First Report ◽  
Mycelial Plug ◽  
Crucial Information ◽  
Filter Papers ◽  
North Vietnam ◽  
Sterile Filter

In August 2019, a collar rot of peanut was observed in several fields in Qingdao, Shandong province, China. Disease survey was conducted in several peanut fields. Less than 5% plants exhibited various symptoms, including brown or black stem rot, pod rot, leaf chlorotic, wilted, and even dead. Symptomatic stems were cut into small pieces, surface disinfested with 70% ethanol for 1 min, 1% NaClO for 2 minutes, rinsed three times with sterile water, and dried on sterile filter papers. Pieces then were plated on potato dextrose agar (PDA) media and incubated at 25°C in darkness. Fungal cultures were initially white, then turned gray, and eventually turned black, and aerial hyphae were dense, fluffy. Conidia were ellipsoidal, initially hyaline, unicellular, 14.3 to 21.1 × 8.7 to 13.2 µm (n = 50), and mature conidia showed dark brown, with a central septum, and longitudinal stripes. Molecular identification was performed by sequencing ITS with ITS1/ITS4 (White et al., 1990) and beta tubulin gene with Bt2a/Bt2b (Glass and Donaldson, 1995) of a representative isolate ZHX9. ITS and beta tubulin regions (OK427342 and OK489788) of ZHX9 obtained 99.62 and 100% similar to L. pseudotheobromae (KF766193 and EU673111), respectively. Phylogenetic analysis was done using Neighbor-Joining (NJ) analysis based on those gene sequences. The microorganism we have isolated was identified as L. pseudotheobromae based on molecular analysis and morphological characteristics. For pathogenicity assay, twelve ten-days-old peanut (Zhonghua No.12) seedlings were each inoculated with one mycelial plug (8 mm in diameter) by placing the inoculum on the base of the stem. Twelve plants were each inoculated with a plug of non-colonized PDA as controls. Plants were incubated in a growth chamber (30°C in the day and 25°C at night, a 12-h photoperiod and 80% RH). Necrotic lesions were observed on stems of all inoculated seedlings 5 days after inoculation, whereas control plants remained asymptomatic, and L. peudotheobromae was consistently re-isolated from symptomatic stem. In Asia, peanut collar rot caused by L. teudotheobromae has been reported in India, Indonesia, North Vietnam (Nguyen, et al., 2006) and China (Guo, et al., 2014), but collar rot caused by L. pseudotheobromae has not been reported. To our knowledge, this is the first report of L. peudotheobromae causing collar rot on peanut in China. These results will provide crucial information for studying on epidemiology and management of this disease.

scholarly journals First report of Geotrichum candidum causing sour rot of peach in China

Plant Disease ◽  
2021 ◽  
Author(s):  
Renxiang Lu ◽  
Zhe Wang ◽  
Yujia Zhai ◽  
Runyu Hong ◽  
Weixin Jin ◽  
...  
Keyword(s):  
Large Scale ◽  
Prunus Persica ◽  
Morphological Characteristics ◽  
Geotrichum Candidum ◽  
Postharvest Disease ◽  
Pathogenicity Test ◽  
Accession Number ◽  
Great Loss ◽  
First Report ◽  
Sour Rot

Peach (Prunus persica L. Batsch) is one of the most important fruit crops in China (Wang et al. 2011). Yangshan Town of Jiangsu Province is one of the four major peach producing areas in China, with a growing area of 2,000 ha (Tian et al. 2018). During June 2020, a postharvest disease presenting with brown necrosis and rot occurred on peaches in Yangshan Town. The estimated damage was more than 10% of the total harvest. The symptoms included soft rot, and the lesion appeared sunken, accompanied with sour odor and white mycelia. Twelve peaches with representative symptom were sampled for pathogen isolation. Pieces (about 5 mm × 5 mm) from the lesion edge of symptomatic fruits were dissected and surface disinfected (3% NaClO for 10 s and 75% ethanol for 30 s), then rinsed three times with distilled water, dried on sterile filter paper and transferred to Potato Dextrose Agar (PDA) media plates supplemented with 150 ng/mL streptomycin sulfate. The plates were incubated at 28 ℃ for 3 days. Forty-eight isolations were obtained from the plates and isolates were single-spored. All isolates presented white, flat, milky yeast-like colonies with radial mycelia. Hyphae under microscope were septate, branched, disarticulating into arthroconidia measuring 3.39 to 9.27 × 2.05 to 7.71 μm. The morphological characteristics are consistent with Geotrichum candidum (De Hoog et al. 1986). Internal transcribed spacer (ITS) and 18s nuclear ribosomal small subunit (SSU) of the 48 isolates were amplified and sequenced using the primers ITS5/ITS4, and NS1/NS4 for molecular identification (Schoch et al. 2012). The resulted sequences showed no difference among all the isolates. Alignment by blastn showed the sequence of ITS and SSU were 100% (accession number. GQ376093) and 99.7% identical (accession number. KY977411.1) to Geotrichum candidum, respectively. The sequences of ITS (accession number MW493646) and SSU (accession number MW493648) were submitted to the GenBank. Commercial ripe peaches with the size of about 15 cm × 15 cm × 10 cm was used for pathogenicity test. Peaches were surface disinfected with 75% ethanol, then a wound with 4 mm in diameter and 5 mm in depth was made on the surface of each fruit. Ten peaches were inoculated with 10 μL (1×105 spores /mL) of the isolate suspension. Another ten peaches were inoculated with 10 μL sterile water as the control. Peaches were incubated individually at 28 ℃and a relative humidity of about 85%. After three days, large scale of pits and necrosis appeared on every peach inoculated, and the symptoms were consistent with the diseased peaches in Yangshan Town, while no symptoms non-inoculated on the control peaches were observed. The pathogen was re-isolated from the diseased fruit and was identified again by sequencing of ITS and SSU. All the tests were conducted three times. Considering the evidence, we identified the pathogen as G. candidum. This pathogen has been reported to cause sour rot was reported in kiwifruit, strawberry, melon and other fruits (Alonzo et al. 2020; Cheng et al. 2020; Halfeld-Vieira et al. 2020). To our knowledge, this is the first report of G. candidum causing sour rot of peach in China, which may cause a great loss to peach industry of China.

Redescription of Orthopristis ruber and Orthopristis scapularis (Haemulidae: Perciformes), with a hybridization zone off the Atlantic coast of South America

Zootaxa ◽  
2019 ◽  
Vol 4576 (1) ◽  
pp. 109 ◽  
Author(s):  
ALEXANDRE PIRES MARCENIUK ◽  
RODRIGO ANTUNES CAIRES ◽  
LEONARDO MACHADO ◽  
NAJILA NOLIE CATARINE DANTAS CERQUEIRA ◽  
RAYLA ROBERTA M. DE S. SERRA ◽  
...  
Keyword(s):  
South America ◽  
Nuclear Dna ◽  
Atlantic Coast ◽  
Morphological Characteristics ◽  
Distinct Species ◽  
Valid Species ◽  
Brazilian Coast ◽  
Northern Coast ◽  
Western Atlantic ◽  
Coloration Patterns

The genus Orthopristis includes seven valid species, three from the western Atlantic and five from eastern Pacific, while the available identification guides and taxonomic keys incorrectly recognize Orthopristis ruber as the only valid species found on the Atlantic coast of South America. Efforts to expand the inventory of fish species from the northern coast of Brazil led to the identification of two distinct species of Orthopristis from Atlantic South America, based on the analysis of coloration patterns and meristic data, as well as DNA. In the present study, the limits of Orthopristis ruber are reviewed, while Orthopristis scapularis is recognized as a valid species for the northern and northeastern coasts of South America. Based on intermediate morphological characteristics and nuclear DNA markers, a hybrid zone was identified off the state of Espírito Santo, on the eastern Brazilian coast. Additionally, considerations are made on the diversity and biogeography of the coastal marine and estuarine fishes found on the Brazilian coast. 

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