Identification and Mapping of QTL for Stripe Rust Resistance in the Chinese Wheat Cultivar Shumai126

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a damaging disease of wheat globally, and breeding resistant cultivars is the best control strategy. The Chinese winter wheat cultivar Shumai126 (SM126) exhibited strong resistance to P. striiformis f. sp. tritici in the field for more than ten years. The objective of this study was to identify and map quantitative trait loci (QTL) for resistance to stripe rust in a population of 154 recombinant inbred lines (RILs) derived from a cross between cultivars Taichang29 (TC29) and SM126. The RILs were tested in six field environments with a mixture of the Chinese prevalent races (CYR32, CYR33, CYR34, Zhong4, and HY46) of P. striiformis f. sp. tritici and in growth chamber with race CYR34 and genotyped using the Wheat55K SNP (single nucleotide polymorphism) array. Six QTL were mapped on chromosomes 1BL, 2AS, 2AL, 6AS, 6BS, and 7BL, respectively. All these QTL were contributed by SM126 except QYr.sicau-2AL. The QYr.sicau-1BL and QYr.sicau-2AS had major effects, explaining 27.00-39.91% and 11.89-17.11% of phenotypic variances, which may correspond to known resistance genes Yr29 and Yr69, respectively. The QYr.sicau-2AL, QYr.sicau-6AS, and QYr.sicau-6BS with minor effects are likely novel. QYr.sicau-7BL was only detected based on growth chamber seedling data. Additive effects were detected for the combination of QYr.sicau-1BL, QYr.sicau-2AS, and QYr.sicau-2AL. SNP markers linked to QYr.sicau-1BL (AX-111056129 and AX-108839316) and QYr.sicau-2AS (AX-111557864 and AX-110433540) were converted to breeder-friendly KASP (Kompetitive allele-specific PCR) markers that would facilitate the deployment of stripe rust resistance genes in wheat breeding.

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Development and Validation of KASP-SNP Markers for QTL Underlying Resistance to Stripe Rust in Common Wheat Cultivar P10057

Plant Disease ◽

10.1094/pdis-04-17-0468-re ◽

2017 ◽

Vol 101 (12) ◽

pp. 2079-2087 ◽

Cited By ~ 12

Author(s):

Jianhui Wu ◽

Qilin Wang ◽

Zhensheng Kang ◽

Shengjie Liu ◽

Haiyang Li ◽

...

Keyword(s):

Stripe Rust ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Wheat Cultivar ◽

Infection Type ◽

Recombinant Inbred Lines ◽

Snp Markers ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Kasp Markers

Stripe rust (Puccinia striiformis f. sp. tritici) is among the most important diseases of wheat (Triticum aestivum L.) globally. Utilization of adult plant resistance (APR) constitutes a key tool for maintaining protection against this disease. The CIMMYT wheat cultivar P10057 displayed a high level of APR to stripe rust in germplasm evaluation in field environments. To clarify the genetic basis and identify quantitative trait loci (QTLs) involved in stripe rust resistance in P10057, three wheat populations were used: 150 F5:6 recombinant inbred lines (RILs) derived from the cross Mingxian 169 × P10057, and 161 and 140 F2:3 lines from Avocet S × P10057 and Zhengmai 9023 × P10057, respectively. These three populations were evaluated for infection type (IT) and disease severity (DS) in Shaanxi, Gansu, and Sichuan during the 2014–15 and 2015–16 cropping seasons. Genotyping was performed with Kompetitive Allelic Specific PCR (KASP) and simple sequence repeat (SSR) markers linked to the resistance loci. Using QTL analysis, two genomic regions associated with resistance were found on chromosome arms 2BS and 3BS, respectively. These two stable QTLs, designated Qyrlov.nwafu-2BS and Qyrlov.nwafu-3BS, were detected across all environments and explained average 22.6 to 31.6% and 21.3 to 32.3% of stripe rust severity phenotypic variation, respectively. Qyrlov.nwafu-2BS may be the resistance allele derived from CIMMYT germplasm and Qyrlov.nwafu-3BS likely corresponds to the locus Sr2/Lr27/Yr30/Pbc. The KASP markers IWA5377, IWA2674, and IWA5830 linked to QYrlov.nwafu-2BS and IWB57990 and IWB6491 linked to Qyrlov.nwafu-3BS were reliable for marker-assisted selection (MAS) in the Zhengmai 9023 × P10057 population. These QTLs with KASP markers are expected to contribute in developing wheat cultivars with improved stripe rust resistance.

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Genome-wide Mapping for Stripe Rust Resistance Loci in Common Wheat Cultivar Qinnong 142

Plant Disease ◽

10.1094/pdis-05-18-0846-re ◽

2019 ◽

Vol 103 (3) ◽

pp. 439-447 ◽

Cited By ~ 4

Author(s):

Qingdong Zeng ◽

Jianhui Wu ◽

Shengjie Liu ◽

Xianming Chen ◽

Fengping Yuan ◽

...

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Wheat Cultivar ◽

Infection Type ◽

Snp Markers ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Wheat Cultivars

Stripe rust caused by Puccinia striiformis f. sp. tritici threatens worldwide wheat production. Growing resistant cultivars is the best way to control this disease. Chinese wheat cultivar Qinnong 142 (QN142) has a high level of adult-plant resistance to stripe rust. To identify quantitative trait loci (QTLs) related to stripe rust resistance, we developed a recombinant inbred line (RIL) population from a cross between QN142 and susceptible cultivar Avocet S. The parents and 165 F6 RILs were evaluated in terms of their stripe rust infection type and disease severity in replicated field tests with six site-year environments. The parents and RILs were genotyped with single-nucleotide polymorphism (SNP) markers. Four stable QTLs were identified in QN142 and mapped to chromosome arms 1BL, 2AL, 2BL, and 6BS. The 1BL QTL was probably the known resistance gene Yr29, the 2BL QTL was in a resistance gene-rich region, and the 2AL and 6BS QTLs might be new. Kompetitive allele specific polymerase chain reaction markers developed from the SNP markers flanking these QTLs were highly polymorphic in a panel of 150 wheat cultivars and breeding lines. These markers could be used in marker-assisted selection for incorporating the stripe rust resistance QTL into new wheat cultivars.

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Barley Stripe Rust Resistance QTL: Development and Validation of SNP Markers for Resistance to Puccinia striiformis f. sp. hordei

Phytopathology ◽

10.1094/phyto-09-15-0225-r ◽

2016 ◽

Vol 106 (11) ◽

pp. 1344-1351 ◽

Cited By ~ 14

Author(s):

K. Esvelt Klos ◽

T. Gordon ◽

P. Bregitzer ◽

P. Hayes ◽

X. M. Chen ◽

...

Keyword(s):

Stripe Rust ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Recombinant Inbred Lines ◽

Snp Markers ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Seedling Resistance ◽

Resistance Qtl ◽

Barley Stripe Rust

Quantitative trait loci (QTL) for barley stripe rust resistance were mapped in recombinant inbred lines (RIL) from a ‘Lenetah’ × ‘Grannelose Zweizeilige’ (GZ) cross. GZ is known for a major seedling resistance QTL on chromosome 4H but linked markers suitable for marker-assisted selection have not been developed. This study identified the 4H QTL (log of the likelihood [LOD] = 15.94 at 97.19 centimorgans [cM]), and additional QTL on chromosomes 4H and 6H (LOD = 5.39 at 72.7 cM and 4.24 at 34.46 cM, respectively). A QTL on chromosome 7H (LOD = 2.04 at 81.07 cM) was suggested. All resistance alleles were derived from GZ. Evaluations of adult plant response in Corvallis, OR in 2013 and 2015 provided evidence of QTL at the same positions. However, the minor QTL on 4H was not statistically significant in either location/year, while the 7H QTL was significant in both. The single-nucleotide polymorphism markers flanking the resistance QTL were validated in RIL from a ‘95SR316A’ × GZ cross for their ability to predict seedling resistance. In 95SR316A × GZ, 91 to 92% of RIL with GZ alleles at the major 4H QTL and at least one other were resistant to moderate in reaction. In these populations, at least two QTL were required to transfer the barley stripe rust resistance from GZ.

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Quantitative Trait Loci Mapping of Adult Plant and Seedling Resistance to Stripe Rust (Puccinia striiformis Westend.) in a Multiparent Advanced Generation Intercross Wheat Population

Frontiers in Plant Science ◽

10.3389/fpls.2021.684671 ◽

2021 ◽

Vol 12 ◽

Author(s):

Sandra Rollar ◽

Manuel Geyer ◽

Lorenz Hartl ◽

Volker Mohler ◽

Frank Ordon ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Quantitative Trait ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Seedling Resistance ◽

Wheat Population ◽

Advanced Generation

Stripe rust caused by the biotrophic fungus Puccinia striiformis Westend. is one of the most important diseases of wheat worldwide, causing high yield and quality losses. Growing resistant cultivars is the most efficient way to control stripe rust, both economically and ecologically. Known resistance genes are already present in numerous cultivars worldwide. However, their effectiveness is limited to certain races within a rust population and the emergence of stripe rust races being virulent against common resistance genes forces the demand for new sources of resistance. Multiparent advanced generation intercross (MAGIC) populations have proven to be a powerful tool to carry out genetic studies on economically important traits. In this study, interval mapping was performed to map quantitative trait loci (QTL) for stripe rust resistance in the Bavarian MAGIC wheat population, comprising 394 F6 : 8 recombinant inbred lines (RILs). Phenotypic evaluation of the RILs was carried out for adult plant resistance in field trials at three locations across three years and for seedling resistance in a growth chamber. In total, 21 QTL for stripe rust resistance corresponding to 13 distinct chromosomal regions were detected, of which two may represent putatively new QTL located on wheat chromosomes 3D and 7D.

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Characterization of Stripe Rust Resistance Genes in the Wheat Cultivar Chuanmai45

International Journal of Molecular Sciences ◽

10.3390/ijms17040601 ◽

2016 ◽

Vol 17 (4) ◽

pp. 601 ◽

Cited By ~ 9

Author(s):

Ennian Yang ◽

Guangrong Li ◽

Liping Li ◽

Zhenyu Zhang ◽

Wuyun Yang ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Wheat Cultivar ◽

Stripe Rust Resistance ◽

Rust Resistance Genes

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Effective Resistance to Wheat Stripe Rust in a Region with High Disease Pressure

Plant Disease ◽

10.1094/pdis-09-13-0909-re ◽

2014 ◽

Vol 98 (7) ◽

pp. 891-897 ◽

Cited By ~ 6

Author(s):

B. Bai ◽

J. Y. Du ◽

Q. L. Lu ◽

C. Y. He ◽

L. J. Zhang ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Hot Spot ◽

Infection Type ◽

Durable Resistance ◽

Gansu Province ◽

Stripe Rust Resistance ◽

Adult Plant

Stripe rust is a major fungal disease of wheat. It frequently becomes epidemic in southeastern Gansu province, a stripe rust hot spot in China. Evaluations of wheat germplasm response are crucial for developing cultivars to control the disease. In total, 57 wheat cultivars and lines from Europe and other countries, comprising 36 cultivars with documented stripe rust resistance genes and 21 with unknown genes, were tested annually with multiple races of Puccinia striiformis f. sp. tritici in the field at Tianshui in Gansu province from 1993 to 2013. Seven wheat lines were highly resistant, with infection type (IT) 0 during the entire period; 16 were moderately resistant (IT 0;-2); and 26 were moderately susceptible (IT 0;-4), with low maximum disease severity compared with the susceptible control Huixianhong. ‘Strampelli’ and ‘Libellula’, with three and five quantitative trait loci, respectively, for stripe rust resistance have displayed durable resistance in this region for four decades. Ten cultivars, including ‘Lantian 15’, ‘Lantian 26’, and ‘Lantian 31’, with stripe rust resistance derived from European lines, were developed in our breeding program and have made a significant impact on controlling stripe rust in southeastern Gansu. Breeding resistant cultivars with multiple adult-plant resistance genes seems to be a promising strategy in wheat breeding for managing stripe rust in this region and other hot spots.

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Seedling and Slow Rusting Resistance to Stripe Rust in Chinese Common Wheats

Plant Disease ◽

10.1094/pd-90-1302 ◽

2006 ◽

Vol 90 (10) ◽

pp. 1302-1312 ◽

Cited By ~ 38

Author(s):

Z. F. Li ◽

X. C. Xia ◽

X. C. Zhou ◽

Y. C. Niu ◽

Z. H. He ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Gene Pyramiding ◽

Stripe Rust Resistance ◽

Slow Rusting ◽

Cropping Seasons ◽

Wheat Lines ◽

Rust Resistance Genes

Identification of seedling and slow stripe rust resistance genes is important for gene pyramiding, gene deployment, and developing slow-rusting wheat cultivars to control the disease. A total of 98 Chinese lines were inoculated with 26 pathotypes of Puccinia striiformis f. sp. tritici for postulation of stripe rust resistance genes effective at the seedling stage. A total of 135 wheat lines were planted at two locations to characterize their slow rusting responses to stripe rust in the 2003-2004 and 2004-2005 cropping seasons. Genes Yr2, Yr3a, Yr4a, Yr6, Yr7, Yr9, Yr26, Yr27, and YrSD, either singly or in combinations, were postulated in 72 lines, whereas known resistance genes were not identified in the other 26 accessions. The resistance genes Yr9 and Yr26 were found in 42 and 19 accessions, respectively. Yr3a and Yr4a were detected in two lines, and four lines may contain Yr6. Three lines were postulated to possess YrSD, one carried Yr27, and one may possess Yr7. Thirty-three lines showed slow stripe rusting resistance at two locations in both seasons.

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Genetic and Molecular Mapping of Stripe Rust Resistance Genes in Wheat Cultivar Zhongliang 12

Journal of Phytopathology ◽

10.1111/jph.12284 ◽

2014 ◽

Vol 163 (2) ◽

pp. 98-104 ◽

Cited By ~ 2

Author(s):

Dongfang Ma ◽

Fei Peng ◽

Zhengwu Fang ◽

Kaixiang Chao ◽

Jinxue Jing ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Molecular Mapping ◽

Wheat Cultivar ◽

Stripe Rust Resistance ◽

Rust Resistance Genes

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Identification of Stripe Rust Resistance Genes in Common Wheat Cultivars From the Huang-Huai-Hai Region of China

Plant Disease ◽

10.1094/pdis-10-19-2119-re ◽

2020 ◽

Vol 104 (6) ◽

pp. 1763-1770

Author(s):

Liang Huang ◽

Xing Zhi Xiao ◽

Bo Liu ◽

Li Gao ◽

Guo Shu Gong ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Molecular Testing ◽

Wheat Cultivars ◽

Rust Resistance Genes ◽

Yr Genes

Wheat stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a serious fungal disease worldwide, especially in the Huang-Huai-Hai region, a main wheat production area in China. Gene postulation, molecular testing, and pedigree analysis were conducted to determine the presence of stripe rust resistance genes to 15 Pst races in 66 selected commercial wheat cultivars released from 2000 to 2016. In addition, races CYR32, CYR33, and CYR34 were used to evaluate resistance to Pst at the adult-plant stage of wheat in the field. Four Yr genes (Yr9, Yr10, Yr26, and Yr32) were postulated in 24 wheat cultivars either singly or in combination. Thirty-six cultivars might contain unknown Yr genes, whereas no identified Yr gene was postulated in six cultivars. Yr9 was detected at a frequency of 28.8%, and no cultivars carried Yr5, Yr15, or Yr18. Ten cultivars (15.2%) exhibited adult-plant resistance in the field tests with three predominant races. Three cultivars (Langyan 43, Xinong 889, and Yunfeng 139) had all-stage resistance. These results are useful to growers selecting cultivars and to breeders aiming to use more resistance genes to develop new cultivars with effective resistance in order to reduce stripe rust damage.

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Utilization of the Genomewide Wheat 55K SNP Array for Genetic Analysis of Stripe Rust Resistance in Common Wheat Line P9936

Phytopathology ◽

10.1094/phyto-10-18-0388-r ◽

2019 ◽

Vol 109 (5) ◽

pp. 819-827 ◽

Cited By ~ 6

Author(s):

Shuo Huang ◽

Jianhui Wu ◽

Xiaoting Wang ◽

Jingmei Mu ◽

Zhi Xu ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Recombinant Inbred Lines ◽

Snp Array ◽

Snp Markers ◽

Stripe Rust Resistance ◽

Biparental Population ◽

Chinese Landrace ◽

Allele Specific ◽

Rust Severity

Breeding for resistance to stripe rust (caused by Puccinia striiformis f. tritici) is essential for reducing losses in yield and quality in wheat. To identify genes for use in breeding, a biparental population of 186 recombinant inbred lines (RILs) from a cross of the Chinese landrace Mingxian 169 and CIMMYT-derived line P9936 was evaluated in field nurseries either artificially or naturally inoculated in two crop seasons. Each of the RILs and parents was genotyped with the wheat 55K single-nucleotide polymorphism (SNP) ‘Breeders’ array and a genetic linkage map with 8,225 polymorphic SNP markers spanning 3,593.37 centimorgans was constructed. Two major quantitative trait loci (QTL) and two minor QTL were identified. The major QTL QYr.nwafu-3BS.2 and QYr.nwafu-7BL on chromosomes arms 3BS and 7BL were detected in all field locations and explained an average 20.4 and 38.9% of phenotypic variation stripe rust severity, respectively. QYr.nwafu-3BS.2 likely corresponds to the locus Yr30/Sr2 and QYr.nwafu-7BL may be a resistance allele identified previously in CIMMYT germplasm. The other minor QTL had limited individual effects but increased resistance when in combinations with other QTL. Markers linked to QYr.nwafu-7BL were converted to kompetitive allele-specific polymerase chain reaction markers and validated in a panel of wheat accessions. Wheat accessions carrying the same haplotype as P9936 at the identified SNP loci had lower average stripe rust severity than the average severity of all other haplotypes.

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