scholarly journals First Report of Virulence and Effects of Magnaporthe oryzae Isolates Causing Wheat Blast in Argentina

Plant Disease ◽  
2015 ◽  
Vol 99 (8) ◽  
pp. 1177 ◽  
Author(s):  
A. Perelló ◽  
I. Martinez ◽  
M. Molina
Plant Disease ◽  
2016 ◽  
Vol 100 (11) ◽  
pp. 2330-2330 ◽  
Author(s):  
P. K. Malaker ◽  
N. C. D. Barma ◽  
T. P. Tiwari ◽  
W. J. Collis ◽  
E. Duveiller ◽  
...  

2021 ◽  
Vol 87 (3) ◽  
pp. 184-191
Author(s):  
Krishna Kanta Roy ◽  
Md Mostofa Ali Reza ◽  
Md Muzahid-E-Rahman ◽  
Kishowar E. Mustarin ◽  
Paritosh Kumar Malaker ◽  
...  

2021 ◽  
Author(s):  
S.I. Martinez ◽  
A. Wegner ◽  
S. Bohnert ◽  
U. Schaffrath ◽  
A. Perello

2018 ◽  
Author(s):  
Dipali Rani Gupta ◽  
Claudia Sarai Reyes Avila ◽  
Joe Win ◽  
Darren M. Soares ◽  
Lauren S. Ryder ◽  
...  

ABSTRACTThe blast fungus Magnaporthe oryzae is comprised of lineages that exhibit varying degrees of specificity on about 50 grass hosts, including rice, wheat and barley. Reliable diagnostic tools are essential given that the pathogen has a propensity to jump to new hosts and spread to new geographic regions. Of particular concern is wheat blast, which has suddenly appeared in Bangladesh in 2016 before spreading to neighboring India. In these Asian countries, wheat blast strains are now co-occurring with the destructive rice blast pathogen raising the possibility of genetic exchange between these destructive pathogens. We assessed the recently described MoT3 diagnostic assay and found that it did not distinguish between wheat and rice blast isolates from Bangladesh. The assay is based on primers matching the WB12 sequence corresponding to a fragment of the M. oryzae MGG_02337 gene annotated as a short chain dehydrogenase. These primers could not reliably distinguish between wheat and rice blast isolates from Bangladesh based on DNA amplification experiments performed in separate laboratories in Bangladesh and in the UK. In addition, comparative genomics of the WB12 sequence revealed a complex underlying genetic structure with related sequences across M. oryzae strains and in both rice and wheat blast isolates. We, therefore, caution against the indiscriminate use of this assay to identify wheat blast.


2019 ◽  
Vol 109 (4) ◽  
pp. 504-508 ◽  
Author(s):  
Dipali Rani Gupta ◽  
Claudia Sarai Reyes Avila ◽  
Joe Win ◽  
Darren M. Soanes ◽  
Lauren S. Ryder ◽  
...  

The blast fungus Magnaporthe oryzae is comprised of lineages that exhibit varying degrees of specificity on about 50 grass hosts, including rice, wheat, and barley. Reliable diagnostic tools are essential given that the pathogen has a propensity to jump to new hosts and spread to new geographic regions. Of particular concern is wheat blast, which has suddenly appeared in Bangladesh in 2016 before spreading to neighboring India. In these Asian countries, wheat blast strains are now co-occurring with the destructive rice blast pathogen raising the possibility of genetic exchange between these destructive pathogens. We assessed the recently described MoT3 diagnostic assay and found that it did not distinguish between wheat and rice blast isolates from Bangladesh. The assay is based on primers matching the WB12 sequence corresponding to a fragment of the M. oryzae MGG_02337 gene annotated as a short chain dehydrogenase. These primers could not reliably distinguish between wheat and rice blast isolates from Bangladesh based on DNA amplification experiments performed in separate laboratories in Bangladesh and in the United Kingdom. Specifically, all eight rice blast isolates tested in this study produced the WB12 amplicon. In addition, comparative genomics of the WB12 nucleotide sequence revealed a complex underlying genetic structure with related sequences across M. oryzae strains and in both rice and wheat blast isolates. We, therefore, caution against the indiscriminate use of this assay to identify wheat blast and encourage further development of the assay to ensure its value in diagnosis.


PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0238724 ◽  
Author(s):  
Batiseba Tembo ◽  
Rabson M. Mulenga ◽  
Suwilanji Sichilima ◽  
Kenneth K. M’siska ◽  
Moses Mwale ◽  
...  

Plant Disease ◽  
2012 ◽  
Vol 96 (8) ◽  
pp. 1228-1228 ◽  
Author(s):  
M. P. You ◽  
V. Lanoiselet ◽  
C. P. Wang ◽  
R. G. Shivas ◽  
Y. P. Li ◽  
...  

Commercial rice crops (Oryza sativa L.) have been recently reintroduced to the Ord River Irrigation Area in northern Western Australia. In early August 2011, unusual leaf spot symptoms were observed by a local rice grower on rice cultivar Quest. A leaf spot symptom initially appeared as grey-green and/or water soaked with a darker green border and then expanded rapidly to several centimeters in length and became light tan in color with a distinct necrotic border. Isolations from typical leaf lesions were made onto water agar, subcultured onto potato dextrose agar, and maintained at 20°C. A representative culture was lodged in the Western Australian Culture Collection Herbarium, Department of Agriculture and Food Western Australia (WAC 13466) and as a herbarium specimen in the Plant Pathology Herbarium, Plant Biosecurity Science (BRIP 54721). Amplification of the internal transcribed spacer (ITS)1 and (ITS)2 regions flanking the 5.8S rRNA gene were carried out with universal primers ITS1 and ITS4 (4). The PCR products were sequenced and BLAST analyses used to compare sequences with those in GenBank. The sequence had 99% nucleotide identity with the corresponding sequence in GenBank for Magnaporthe oryzae B.C. Couch, the causal agent of rice blast, the most important fungal disease of rice worldwide (1). Additional sequencing with the primers Bt1a/Bt1b for the β-tubulin gene, primers ACT-512F/ACT-783R for the actin gene, and primers CAL-228F/CAL-737R for the calmodulin gene showed 100% identity in each case with M. oryzae sequences in GenBank, confirming molecular similarity with other reports, e.g., (1). The relevant sequence information for a representative isolate has been lodged in GenBank (GenBank Accession Nos. JQ911754 for (ITS) 1 and 2; JX014265 for β-tubulin; JX035809 for actin; and JX035808 for calmodulin). Isolates also showed morphological similarity with M. oryzae as described in other reports, e.g., (3). Spores of M. oryzae were produced on rice agar under “black light” at 21°C for 4 weeks. Under 30/28°C (day/night), 14/12 h (light/dark), rice cv. Quest was grown for 7 weeks, and inoculated by spraying a suspension 5 × 105 spores/ml onto foliage until runoff occurred. Inoculated plants were placed under a dark plastic covering for 72 h to maximize humidity levels around leaves, and subsequently maintained under >90% RH conditions. Typical symptoms of rice blast appeared within 14 days of inoculation and were as described above. Infection studies were successfully repeated and M. oryzae was readily reisolated from leaf lesions. No disease symptoms were observed nor was M. oryzae isolated from water-inoculated control rice plants. There have been previous records of rice blast in the Northern Territory (2) and Queensland, Australia (Australian Plant Pest Database), but this is the first report of M. oryzae in Western Australia, where it could potentially be destructive if conditions prove conducive. References: (1) B. C. Couch and L. M. Kohn. Mycologia 94:683, 2002; (2) J. B. Heaton. The Aust. J. Sci. 27:81, 1964; (3) C. V. Subramanian. IMI Descriptions of Fungi and Bacteria No 169, Pyricularia oryzae, 1968; (4) T. J. White et al. PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds. Academic Press, New York, 1990.


Author(s):  
MH Kabir ◽  
HR Nayan ◽  
MA Abedin ◽  
MB Meah

Wheat blast (Magnaporthe oryzae Triticum) in Bangladesh and South America is recognized as one major limiting factor of wheat production. Its control using chemical pesticides raises concerns about food safety and pesticide resistance, which have dictated the need for alternative blast management approach, nutrient supplementation could be an ecofriendly alternative. Experiments were carried out under confined net house condition for two consecutive cropping seasons. Single doses of the nutrients (Si, B and Zn) were incorporated during soil preparation. Plants of the wheat blast susceptible variety BARI Gom-26 were inoculated with spores (1 x 107 spores ml-1) of Magnaporthe oryzae Triticum at blast vulnerable pre-heading stage of 52 days age. Typical wheat blast symptoms of spike bleaching from top to downward appeared on sight 14 days after inoculation i.e., 66 days age of the crop. Incidence and severity of blast bleaching of spike were scored for four times starting from 68 days age @ three day’s interval. None of the nutrients could stop the incidence of blast on wheat; however, some nutrients reduced the blast incidence significantly. Solo application of Si, B and Zn or combination of two caused significant reduction of spike bleaching. With the mixed application of Si, B and Zn, > 47% reduction of wheat blast severity was obtained. The results revealed that the soil application of silicon, zinc and boron had a synergistic effect on the intensity of blast disease of wheat. Int. J. Agril. Res. Innov. Tech. 11(2): 76-84, Dec 2021


Author(s):  
Chandra Shekhar Biswas ◽  
Afsana Hannan ◽  
Abul Monsur ◽  
G H M Sagor

Global food security is seriously threatened due to increased frequency and occurrence of fungal diseases. One example is wheat blast caused by Magnaporthe oryzae is a fungal diseases of rice, wheat, and other grasses, that can destroy the whole food production to sustain millions of people. Wheat blast was first detected in february 2016 with a serious outbreak in Asia. Assessment of the available germplasms to stress tolerant/resistant is one of the best options for developing stress tolerant crop varieties. In this study, a total of sixteen wheat cultivars were collected and test their disease severity to blast pathogen Magnaporthe oryzae pv. Triticum (MoT). Among the varieties, BARI Gom 33 exhibited partially resistance against blast pathogen, whereas all other genotypes become susceptible to MoT. Different yield and yield contributing characters of both resistant and susceptible cultivars were also evaluated and found no significant differences among them. To understand the underlying mechanism of resistance in BARI Gom 33, antioxidant enzyme activity, concentration of reactive oxygen species and cellular damage after fungal infection were also evaluated and found that activities of ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POD) were higher in BARI Gom 33 than BARI Gom 25 and BARI Gom 31. The hydrogen peroxide (H2O2) and malondealdehyde (MDA) content in BARI Gom 33 was low compare to BARI Gom 25 and BARI Gom 31, which may due to greater increase of the APX, CAT and POD in resistant genotypes. Thus, it may suggest that a more efficient antioxidative defense system in BARI Gom 33 during the infection process of M. oryzae restricts the cell damage caused by the fungus. The identified genotypes can either be used directly in the blast prone area or as a source of resistance to further development of blast resistance high yielding wheat variety.


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