scholarly journals Epidemiology of Xiphinema americanum and Tomato ringspot virus on Red Raspberry, Rubus idaeus

Plant Disease ◽  
2008 ◽  
Vol 92 (3) ◽  
pp. 364-371 ◽  
Author(s):  
J. N. Pinkerton ◽  
J. Kraus ◽  
R. R. Martin ◽  
R. P. Schreiner
Keyword(s):  
Pacific Northwest ◽  
Rubus Idaeus ◽  
Ringspot Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infested Soil ◽  
Red Raspberry ◽  
Rate Of Spread ◽  
Tomato Ringspot Virus ◽  
Systemic Spread ◽  
Gravid Females

Population dynamics of Xiphinema americanum and transmission of Tomato ringspot virus (ToRSV) were studied in a red raspberry field in Washington State. Population densities of X. americanum were highest in the winter, lowest in the summer, and were correlated with precipitation (R2 = 0.42). All nematode stages were present throughout the year. Gravid females were observed only in the spring, indicating one generation per year. The sequence of the coat protein of the ToRSV from this field was similar to those of other raspberry isolates from the Pacific Northwest. Cucumber seedlings were planted in soil collected monthly from the field and were evaluated for nematode transmission of ToRSV by enzyme-linked immunosorbent assay (ELISA). The proportion of assay plants infected with ToRSV was negatively correlated with nematode densities (R2 = 0.31). In another study, ToRSV was detected by ELISA in fine roots of raspberry plants 5 months after planting in field soil infested with viruliferous nematodes, in all subterranean portions of plants after 12 months, and in all aerial portions the second year. The rate of spread of ToRSV in a raspberry field was 70 cm per year. These results suggest that the rate of ToRSV spread is limited by systemic spread of virus in plants when nematode-infested soil is not transported in the field.

EFFECTS OF TOMATO RINGSPOT VIRUS ON THE GROWTH AND YIELD OF RED RASPBERRY

1975 ◽  
Vol 55 (3) ◽  
pp. 749-754 ◽  
Author(s):  
JACK A. FREEMAN ◽  
R. STACE-SMITH ◽  
HUGH A. DAUBENY
Keyword(s):  
Growth Parameters ◽  
Fruit Size ◽  
Rubus Idaeus ◽  
Ringspot Virus ◽  
Growth And Yield ◽  
Red Raspberry ◽  
Tomato Ringspot Virus

Tomato ringspot virus (TomRSV) was graft-inoculated into 10 raspberry (Rubus idaeus L.) cultivars in 1970 and plants of root cuttings from infected and virus-free (VF) plants were established in the field in 1971. Data were recorded on the growth of the newly propagated plants in 1971 and on growth and yield in 1972, 1973 and 1974. TomRSV infection resulted in retarding the growth of the young plants arising from the root cuttings of 8 of the 10 cultivars. Chlorotic ringspot markings were evident on many of the young plants arising from the root cuttings, but in subsequent years very few foliar markings were observed. A year after the plants were established, the canes of six cultivars were shorter than those of VF clones. The cultivars Avon, Latham, Lloyd George, and Mailing Jewel had significantly shorter canes in each of the 3 yr that measurements were taken. Only two cultivars, Carnival and Lloyd George, showed a reduction in yield in the 1st cropping year. By the 3rd cropping year, Avon, Glen Clova, Latham, Lloyd George and Meeker all showed a significant reduction in yield. Avon and Lloyd George also showed reduced fruit size. When all growth parameters were considered, the cultivar ranking in order of decreasing susceptibility was as follows: Lloyd George, Avon, Latham, Glen Clova, Meeker, Matsqui, Mailing Jewel, Carnival, Puyallup, and Canby.

scholarly journals EFFECTS OF TOMATO RINGSPOT VIRUS ON DRUPELET SET OF RED RASPBERRY CULTIVARS

1975 ◽  
Vol 55 (3) ◽  
pp. 755-759 ◽  
Author(s):  
HUGH A. DAUBENY ◽  
R. STACE-SMITH ◽  
JACK A. FREEMAN
Keyword(s):  
Adverse Effects ◽  
Male Fertility ◽  
Rubus Idaeus ◽  
Female Fertility ◽  
Ringspot Virus ◽  
Red Raspberry ◽  
Male And Female ◽  
Pollen Quality ◽  
Tomato Ringspot Virus

Tomato ringspot virus (Tom RSV) had particularly adverse effects on drupelet set of the red raspberry (Rubus idaeus L.) cultivars Avon and Lloyd George. It affected Matsqui, Puyallup and Newburgh to a lesser extent. In Tom RSV-infected Newburgh, reduced drupelet set occurred only on those plants showing decline symptoms. With Avon and Lloyd George, reduced drupelet set appeared to involve reductions in male and female fertility. In Matsqui, Puyallup and Newburgh, male fertility as determined by pollen quality did not seem to be affected. Tom RSV did not affect drupelet set of Canby, Carnival, Glen Clova, Latham, Malling Jewel and Meeker.

scholarly journals Salicylic Acid-Dependent Restriction of Tomato ringspot virus Spread in Tobacco Is Accompanied by a Hypersensitive Response, Local RNA Silencing, and Moderate Systemic Resistance

2011 ◽  
Vol 24 (6) ◽  
pp. 706-718 ◽  
Author(s):  
Juan Jovel ◽  
Melanie Walker ◽  
Hélène Sanfaçon
Keyword(s):  
Salicylic Acid ◽  
Hypersensitive Response ◽  
Rna Silencing ◽  
Lesion Size ◽  
Ringspot Virus ◽  
Viral Rna ◽  
Systemic Resistance ◽  
Tomato Ringspot Virus ◽  
Systemic Spread ◽  
Transgenic Lines

Tomato ringspot virus (ToRSV, a Nepovirus sp.) systemically infects many herbaceous plants. Viral RNA accumulates in symptomatic leaves and in young, asymptomatic leaves that emerge late in infection. Here, we show that systemic infection by ToRSV is restricted in tobacco. After an initial hypersensitive response in inoculated leaves, only a few plants showed limited systemic symptoms. Viral RNA did not usually accumulate to detectable levels in asymptomatic leaves. ToRSV-derived small-interfering RNAs and PR1a transcripts were only detected in tissues that contained viral RNA, indicating local induction of RNA silencing and salicylic acid (SA)-dependent defense responses. Lesion size and viral systemic spread were reduced with SA pretreatment but enhanced in NahG transgenic lines deficient in SA accumulation, suggesting that SA-dependent mechanisms play a key role in limiting ToRSV spread in tobacco. Restriction of virus infection was enhanced in transgenic lines expressing the P1-HC-Pro suppressor of silencing. Knocking down the SA-inducible RNA-dependent RNA polymerase 1 exacerbated the necrotic reaction but did not affect viral systemic spread. ToRSV-infected tobacco plants were susceptible to reinoculation by ToRSV or Tobacco mosaic virus, although a small reduction in lesion size was observed. This moderate systemic resistance suggests inefficient induction or spread of RNA silencing and systemic acquired resistance signal molecules.

scholarly journals Caneburning Effects on Weeds, Primocane Growth, and Fruit Yield in Pacific Northwest Red Raspberry

HortScience ◽  
2017 ◽  
Vol 52 (5) ◽  
pp. 692-699
Author(s):  
Yushan Duan ◽  
Thomas W. Walters ◽  
Timothy W. Miller
Keyword(s):  
Weed Control ◽  
Pacific Northwest ◽  
Early Spring ◽  
Rubus Idaeus ◽  
Fruit Yield ◽  
Average Yield ◽  
Red Raspberry ◽  
Residual Herbicide ◽  
Yield Trials ◽  
Commercial Field

A large percentage of North American processing red raspberry (Rubus idaeus L.) is produced in maritime areas of western regions in Washington, British Columbia, and Oregon. Most of these raspberry plantings are caneburned; they are treated with herbicides in early spring to remove the first-emerging primocanes in an attempt to reduce their competition with floricanes during flowering and fruiting and thereby increase fruit yield. Trials were conducted in northwestern Washington to determine the effect of carfentrazone and oxyfluorfen applied for primocane management in three currently grown raspberry cultivars. Although caneburning did provide early season weed control in treated plots, weed control fell below 70% in the commercial ‘Meeker’ field more quickly each year: at 138 days after treatment (DAT) in 2010, 71 DAT in 2011, and 47 DAT in 2012, compared with greater than 80% control at the end of each season when the soil residual herbicide terbacil was applied. Oxyfluorfen provided longer suppression of primocane growth than did carfentrazone, particularly in ‘Cascade Bounty’ but also to a lesser extent in ‘Meeker’. Caneburning did not improve raspberry yield in five of the six tested years for ‘Meeker’, or in any of the five tested years for ‘Cascade Bounty’ or the single year for ‘Coho’, although the trend was for greater yield with caneburning across all cultivars. Oxyfluorfen improved 3-year ‘Meeker’ average yield 43% from nontreated raspberry in the commercial field trial, compared with a 29% increase with carfentrazone.

scholarly journals First Report of Raspberry bushy dwarf virus on Red Raspberry and Grapevine in Slovenia

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1148-1148 ◽  
Author(s):  
I. Mavrič ◽  
M. Viršček Marn ◽  
D. Koron ◽  
I. Žežlina
Keyword(s):  
Polyclonal Antiserum ◽  
Rubus Idaeus ◽  
Dwarf Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Natural Occurrence ◽  
Red Raspberry ◽  
Rt Pcr ◽  
First Report ◽  
Raspberry Bushy Dwarf Virus ◽  
Das Elisa

In 2002, severe vein yellowing and partial or complete yellowing of leaves was observed on some shoots of red raspberry (Rubus idaeus) cvs. Golden Bliss and Autumn Bliss. Sap of infected plants of cv. Golden Bliss was inoculated onto Chenopodium quinoa and Nicotiana benthamiana. Faint chlorotic spots were observed on inoculated leaves of C. quinoa approximately 14 days after inoculation but no systemic symptoms appeared. No symptoms were observed on N. benthamiana. Raspberry bushy dwarf virus (RBDV) was detected in the original raspberry plant using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with polyclonal antiserum (Loewe Biochemica, Sauerlach, Germany). Systemic infections of inoculated C. quinoa and N. benthaminana were confirmed using DAS-ELISA. In 2001 and 2002, unusual virus symptoms were observed on grapevine grafts (Vitis vinifera) of cv. Laški Rizling. Symptoms appeared as curved line patterns and yellowing of the leaves. No nepoviruses were found in symptomatic plants, but RBDV was confirmed using DAS-ELISA. RBDV infection was later confirmed in grapevine cv. Štajerska Belina with similar symptoms. RBDV was transmitted mechanically from grapevine to C. quinoa where it was detected by immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR). IC-RT-PCR was used to amplify a part of the coat protein gene of the virus from raspberry and grapevine, and the amplification products were sequenced (1). The obtained sequence shared at least 93% nucleotide sequence identity with other known RBDV sequences, which confirmed the serological results. To our knowledge, this is the first report of the natural occurrence of RBDV in grapevine and also of RBDV infection of red raspberry in Slovenia. Reference: (1) H. I. Kokko et al. Biotechniques 20:842, 1996.

scholarly journals Transformation of Plum with the Papaya Ringspot Virus Coat Protein Gene and Reaction of Transgenic Plants to Plum Pox Virus

1995 ◽  
Vol 120 (6) ◽  
pp. 943-952 ◽  
Author(s):  
Ralph Scorza ◽  
Laurene Levy ◽  
Vern Damsteegt ◽  
Luz Marcel Yepes ◽  
John Cordts ◽  
...  
Keyword(s):  
Coat Protein ◽  
Kanamycin Resistance ◽  
Ringspot Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plum Pox Virus ◽  
Papaya Ringspot Virus ◽  
Indolebutyric Acid ◽  
Systemic Spread ◽  
Foreign Genes ◽  
Virus Coat

Transgenic plum plants expressing the papaya ringspot virus (PRV) coat protein (CP) were produced by Agrobacterium-mediated transformation of hypocotyl slices. Hypocotyl slices were cocultivated with Agrobacterium tumefaciens strain C58/Z707 containing the plasmid pGA482GG/CPPRV-4. This plasmid carries the PRVCP gene construct and chimeric NPTII and GUS genes. Shoots were regenerated on Murashige and Skoog salts, vitamins, 2% sucrose, 2.5 μm indolebutyric acid, 7.5 μm thidiazuron, and appropriate antibiotics for selection. Integration of the foreign genes was verified through kanamycin resistance, GUS assays, polymerase chain reaction (PCR), and Southern blot analyses. Four transgenic clones were identified. Three were vegetatively propagated and graft-inoculated with plum pox virus (PPV)-infected budwood in a quarantine, containment greenhouse. PPV infection was evaluated over a 2- to 4-year period through visual symptoms, enzyme-linked immunosorbent assay, and reverse transcriptase PCR assays. While most plants showed signs of infection and systemic spread of PPV within l-6 months, one plant appeared to delay the spread of virus and the appearance of disease symptoms. Virus spread was limited to basal portions of this plant up to 19 months postinoculation, but, after 32 months symptoms were evident and virus was detected throughout the plant. Our results suggest that heterologous protection with PRVCP, while having the potential to delay PPV symptoms and spread throughout plum plants, may not provide an adequate level of long-term resistance.

scholarly journals Post-Plant Nematicides for the Control of Root Lesion Nematode in Red Raspberry

2010 ◽  
Vol 20 (5) ◽  
pp. 856-862 ◽  
Author(s):  
Inga A. Zasada ◽  
Thomas W. Walters ◽  
John N. Pinkerton
Keyword(s):  
Foliar Application ◽  
Rubus Idaeus ◽  
Infested Soil ◽  
Pratylenchus Penetrans ◽  
Root Lesion Nematode ◽  
Red Raspberry ◽  
Population Densities ◽  
Quillaja Saponaria ◽  
Lesion Nematode ◽  
Sampling Times

To identify a post-plant nematicide to control root lesion nematode [RLN (Pratylenchus penetrans)] in red raspberry (Rubus idaeus), a number of nematicides was tested in soil-only and plant-based experiments. In soil-only experiments, soil naturally infested with RLN was drenched with the nematicides and nematode survival was assessed 7 and 14 days after treatment. Fosthiazate and oxamyl reduced RLN recovery 92% and 52% across trials and sampling times, respectively, compared with the nontreated control. Other nematicides that resulted in moderate, and sometimes inconsistent, control of RLN were soapbark (Quillaja saponaria) saponins, 1,3-dichloropropene, and methomyl. In plant-based experiments, ‘Meeker’ red raspberry was established in pots with RLN-infested soil mixed with greenhouse soil and the nematicides were applied as soil drenches or as a foliar application. Nematode recovery and cane and root weights were quantified as measurements of nematicide toxicity and phytotoxicity, respectively. Similar to soil-only experiments, fosthiazate and oxamyl were the most effective nematicides tested in reducing RLN population densities in established red raspberry. Fosthiazate and oxamyl significantly reduced RLN per gram dry root population densities by 97% and 87%, respectively, compared with the infested, nontreated control. None of the other nematicides reduced RLN population densities compared with the infested, nontreated controls. There was no phytotoxicity to red raspberry associated with any of the nematicides.

scholarly journals Distinguishing Pacific Northwest Red Raspberry Cultivars using RAPD Markers

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 833G-833 ◽  
Author(s):  
Patrick P. Moore
Keyword(s):  
Pacific Northwest ◽  
Genetic Map ◽  
Dna Markers ◽  
Rapd Markers ◽  
Rubus Idaeus ◽  
Randomly Amplified Polymorphic Dna ◽  
Red Raspberry ◽  
Base Sequences

Randomly amplified polymorphic DNA markers (RAPDs) were used to distinguish among seven Pacific Northwest red raspberry (Rubus idaeus L.) cultivars. Random 10-base sequences were used to distinguish among `Chilcotin', `Chilliwack', `Comox', `Meeker', `Qualicum', `Tulameen', and `Willamette'. The seven cultivars could be distinguished even though there is considerable relatedness among the cultivars. `Chilliwack' and `Comox' share `Skeena' as a parent, and `Chilliwack' is a parent of `Qualicum'. `Willamette' is a parent of `Meeker'. This technology shows promise as a means of distinguishing cultivars and developing a genetic map to aid in breeding.

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