scholarly journals Quantitative Resistance to Bean dwarf mosaic virus in Common Bean Is Associated with the Bct Gene for Resistance to Beet curly top virus

Plant Disease ◽  
2009 ◽  
Vol 93 (6) ◽  
pp. 645-648 ◽  
Author(s):  
P. N. Miklas ◽  
Y.-S. Seo ◽  
R. L. Gilbertson
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Quantitative Resistance ◽  
Bipartite Begomovirus ◽  
Beet Curly Top Virus ◽  
Host Interaction ◽  
Bean Plants ◽  
Qualitative Resistance ◽  
The Difference ◽  
Bean Dwarf Mosaic Virus

The dominant resistance gene, Bct, in common bean (Phaseolus vulgaris) confers qualitative resistance to Beet curly top virus, a leafhopper-transmitted geminivirus in the genus Curtovirus. To determine whether this gene confers resistance to other geminiviruses, bean plants of a recombinant inbred population were sap-inoculated with Bean dwarf mosaic virus (BDMV), a whitefly-transmitted bipartite begomovirus in the genus Begomovirus. Results indicated that Bct (or tightly linked gene) is associated with quantitative resistance to BDMV; thus, the Bct locus is associated with resistance to a bean-infecting begomovirus and curtovirus. The difference in the nature of the resistance to these geminiviruses may indicate a role for minor genes in begomovirus resistance or differences in the virus–host interaction. The Bct locus, whether it acts alone or represents a cluster of tightly linked genes, will be useful in breeding for broad-spectrum begomovirus resistance in common bean.

scholarly journals Next-Generation Sequencing-Based Detection of Common Bean Viruses in Wild Plants from Tanzania and Their Mechanical Transmission to Common Bean Plants

Plant Disease ◽  
2021 ◽  
Author(s):  
Beatrice Mwaipopo ◽  
Minna-Liisa Rajamäki ◽  
Neema Ngowi ◽  
Susan N’chimbi Msolla ◽  
P Njau ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Common Bean ◽  
Mosaic Virus ◽  
Viral Disease ◽  
Wild Plants ◽  
Next Generation ◽  
Rt Pcr ◽  
Bean Plants ◽  
Generation Sequencing ◽  
Common Bean Plants

Viral diseases are a major threat for common bean production. In recent surveys, >15 different viruses belonging to 11 genera were shown to infect common bean (Phaseolus vulgaris L.) in Tanzania. Management of viruses requires an understanding of how they survive from one season to the next. In this study, we explored the possibility that alternative host plants have a central role in the survival of common bean viruses. We used next-generation sequencing (NGS) techniques to sequence virus-derived small interfering RNAs, together with conventional reverse transcription-polymerase chain reaction (RT-PCR) to detect viruses in wild plants. Leaf samples for RNA extraction and NGS were collected from 1,430 wild plants around and within common bean fields in four agricultural zones in Tanzania. At least partial genome sequences of viruses potentially belonging to 25 genera were detected. The greatest virus diversity was detected in the eastern and northern zones, whereas wild plants in the Lake zone and especially in the southern highlands zone showed only a few viruses. RT-PCR analysis of all the collected plant samples confirmed the presence of yam bean mosaic virus and peanut mottle virus in wild legume plants. Of all viruses detected, only two viruses, cucumber mosaic virus and a novel bromovirus related to cowpea chlorotic mottle virus and brome mosaic virus, were mechanically transmitted from wild plants to common bean plants. The data generated in this study are crucial for development of viral disease management strategies and predicting crop viral disease outbreaks in different agricultural regions in Tanzania and beyond.

Analysis of maize–common bean intercrops in semi-arid Kenya

1994 ◽  
Vol 123 (2) ◽  
pp. 191-198 ◽  
Author(s):  
C. J. Pilbeam ◽  
J. R. Okalebo ◽  
L. P. Simmonds ◽  
K. W. Gathua
Keyword(s):  
Common Bean ◽  
Plant Density ◽  
Research Station ◽  
Land Equivalent Ratio ◽  
Bean Plants ◽  
Plant Densities ◽  
The Mean ◽  
The Difference ◽  
Short Rains ◽  
Seed Yields

SummaryMaize (Zea mays L.) and common bean (Phaseolus vulgaris L.) were each sown at four plant densities, including zero, in a bivariate factorial design at Kiboko Rangeland Research Station, Kenya during the long and short rains of 1990. The design gave nine intercrops with different proportions of maize and beans, and six sole crops, three of maize and three of beans. Seed yields in both the sole crops were not significantly affected by plant density, so the mean yield was used to calculate the Land Equivalent Ratio (LER), which averaged 1·09 in the long rains but only 0·87 in the short rains. These low values were apparently due to the fact that beans failed to nodulate and fix nitrogen in the study area. The difference in LER between seasons was probably caused by differences in the amount and distribution of rain in relation to crop growth. Maize was more competitive than bean, each maize plant being equivalent to between 0·7 and 3·4 bean plants depending upon the treatment and the season.

scholarly journals Cuban Isolate of Bean golden yellow mosaic virus is a Member of the Mesoamerican BGYMV Group

Plant Disease ◽  
2001 ◽  
Vol 85 (9) ◽  
pp. 1030-1030 ◽  
Author(s):  
A. L. Echemendía ◽  
P. L. Ramos ◽  
R. Peral ◽  
A. Fuentes ◽  
G. González ◽  
...  
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Virus Isolate ◽  
Iterative Sequence ◽  
Yellow Mosaic Virus ◽  
Economic Losses ◽  
Polymorphism Analysis ◽  
Southern Mexico ◽  
Bean Plants ◽  
Golden Yellow

In Cuba, the emergence of bean golden mosaic was associated with high populations of Bemisia tabaci in common bean (Phaseolus vulgaris L.) plantings in the 1970s (1). During the last two decades, the disease has caused significant economic losses, forcing some growers to abandon bean production. In Holguín, one of the main bean producing provinces of the country, about 2,000 ha of beans were abandoned in 1991 due to the high incidence of this whitefly-transmitted virus. At that time, yield losses associated with this disease reached 90 to 100% in farmer's fields. In spite of various control measures, the disease affected 33, 28, and 6.5% of the total area planted in Cuba to common bean in 1990, 1992, and 1996, respectively. For this investigation, common bean leaves showing systemic yellowing symptoms were collected in fields located in the provinces of Havana, Matanzas, and Holguín during 1998-1999. Sap and total DNA leaf extracts were used to inoculate healthy bean plants by manual and biolistic procedures, respectively. Characteristic yellowing symptoms were more efficiently reproduced using a particle gun device than by manual inoculation (18/20 plants and 5/20 plants, respectively, for a Holguín virus isolate). DNA extracts were further analyzed by polymerase chain reaction using two degenerate primer sets: PAL1v1978-PAR1c715 and PAL1c1960-PAR1v722 (2). Fragments of approximately 1.4 and 1.2 kb were amplified and cloned. Restriction fragment length polymorphism analysis of the cloned 1.4-kb fragments was performed with BglII, HincII, SalI, EcoRI, PstI, and XbaI, indicating that selected isolates from the three Cuban provinces shared identical restriction patterns. The nucleotide sequence obtained from two clones of a virus isolate from Holguín, was compared to sequences available for other begomoviruses using BLAST. The Cuban isolate shared up to 94% nt sequence identity with various strains of Bean golden yellow mosaic virus (BGYMV) in the first 250 nt of the rep gene. For the common region (CR), scores were 93% for BGYMV-GA (Guatemala), 92% for BGYMV-MX (southern Mexico) and BGYMV-PR (Puerto Rico), and 91% for BGYMV-DR (Dominican Republic). The iterative sequence ATGGAG was identified in the CR of the Cuban BGYMV isolate, as reported for other BGYMV isolates. Finally, the Cuban begomovirus, hereafter referred to as BGYMV-CU, shared nt and aa sequence identities of 94 and 100%, respectively, with the coat protein gene of BGYMV-MX. We conclude that the begomovirus isolated from mosaic-affected common bean plants in the province of Holguín is a member of the Mesoamerican BGYMV group (3). References: (1) N. Blanco and C. Bencomo. Cienc. Agric. 2:39, 1978. (2) M. R. Rojas et al. Plant Dis. 77:340, 1993. (3) Morales and Anderson, Arch. Virol. 146:415, 2001.

scholarly journals The N-terminus of the Begomovirus Nuclear Shuttle Protein (BV1) Determines Virulence or Avirulence in Phaseolus vulgaris

2007 ◽  
Vol 20 (12) ◽  
pp. 1523-1534 ◽  
Author(s):  
Y.-C. Zhou ◽  
E. R. Garrido-Ramirez ◽  
M. R. Sudarshana ◽  
S. Yendluri ◽  
R. L. Gilbertson
Keyword(s):  
Amino Acids ◽  
Common Bean ◽  
Mosaic Virus ◽  
Bipartite Begomoviruses ◽  
Yellow Mosaic Virus ◽  
Bipartite Begomovirus ◽  
Nuclear Shuttle Protein ◽  
Variable Surface ◽  
Begomovirus Genome ◽  
N Terminus

The BV1 gene of the bipartite Begomovirus genome encodes a nuclear shuttle protein (NSP) that is also an avirulence determinant in common bean. The function of the NSP of two common bean-infecting bipartite begomoviruses, Bean dwarf mosaic virus (BDMV) and Bean golden yellow mosaic virus (BGYMV), was investigated using a series of hybrid DNA-B components expressing chimeric BDMV and BGYMV NSP, and genotypes of the two major common bean gene pools: Andean (cv. Topcrop) and Middle American (cvs. Alpine and UI 114). BDMV DNA-A coinoculated with HBDBG4 (BDMV DNA-B expressing the BGYMV NSP) and HBDBG9 (BDMV DNA-B expressing a chimeric NSP with the N-terminal 1 to 42 amino acids from BGYMV) overcame the BDMV resistance of UI 114. This established that the BDMV NSP is an avirulence determinant in UI 114, and mapped the domain involved in this response to the N-terminus, which is a variable surface-exposed region. BDMV DNA-A coinoculated with HBDBG10, expressing a chimeric NSP with amino acids 43 to 92 from BGYMV, was not infectious, revealing an essential virus-specific domain. In the BGYMV background, the BDMV NSP was a virulence factor in the Andean cv. Topcrop, whereas it was an avirulence factor in the Middle American cultivars, particularly in the absence of the BGYMV NSP. The capsid protein (CP) also played a gene pool–specific role in viral infectivity; it was dispensable for infectivity in the Andean cv. Topcrop, but was required for infectivity of BDMV, BGYMV, and certain hybrid viruses in the Middle American cultivars. Redundancy of the CP and NSP, which are nuclear proteins involved directly or indirectly in viral movement, provides a masking effect that may allow the virus to avoid host defense responses.

Indigenous rhizobial strains SEMIA 4108 and SEMIA 4107 for common bean inoculation: A biotechnological tool for cleaner and more sustainable agriculture

2021 ◽  
pp. 1-11
Author(s):  
Bruno Britto Lisboa ◽  
Thomas Müller Schmidt ◽  
Arthur Henrique Ely Thomé ◽  
Raul Antonio Sperotto ◽  
Camila Gazolla Volpiano ◽  
...  
Keyword(s):  
Grain Yield ◽  
Common Bean ◽  
Field Experiments ◽  
Rhizobium Phaseoli ◽  
Productivity Losses ◽  
Bean Plants ◽  
Rrna Sequencing ◽  
Low Efficiency ◽  
Type Strains ◽  
Common Bean Plants

Summary Inoculation of symbiotic N2-fixing rhizobacteria (rhizobia) in legumes is an alternative to reduce synthetic N fertiliser input to crops. Even though common bean benefits from the biological N2 fixation carried out by native rhizobia isolates, the low efficiency of this process highlights the importance of screening new strains for plant inoculation. Two rhizobial strains (SEMIA 4108 and SEMIA 4107) previously showed great potential to improve the growth of common beans under greenhouse conditions. Thus, this study evaluated the growth and grain yield of common bean plants inoculated with those strains in field experiments. The rhizobial identification was performed by 16S rRNA sequencing and the phylogeny showed that SEMIA 4108 and SEMIA 4107 are closely related to Rhizobium phaseoli, within a clade containing other 18 Rhizobium spp. type strains. Common bean plants inoculated with SEMIA 4107 showed similar productivity to N-fertilised (N+) plants in the first experiment (2016/17) and higher productivity in the second experiment (2018/19). The development of inoculated plants was different from that observed for N+. Nonetheless, comparing inoculated treatments with N-fertilised control, no yield or productivity losses at the end of the growing process were detected. Our results showed that inoculation of the rhizobial isolates SEMIA 4108 and SEMIA 4107 improved the growth and grain yield of common bean plants. The observed agronomical performance confirms that both strains were effective and can sustain common bean growth without nitrogen fertilisation under the edaphoclimatic conditions of this study.

The visualization of local lesions caused by the common bean mosaic virus

1972 ◽  
Vol 14 (5) ◽  
pp. 369-370 ◽  
Author(s):  
J. Polák ◽  
J. Chod
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Local Lesions ◽  
The Common
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