scholarly journals Cuban Isolate of Bean golden yellow mosaic virus is a Member of the Mesoamerican BGYMV Group

Plant Disease ◽  
2001 ◽  
Vol 85 (9) ◽  
pp. 1030-1030 ◽  
Author(s):  
A. L. Echemendía ◽  
P. L. Ramos ◽  
R. Peral ◽  
A. Fuentes ◽  
G. González ◽  
...  
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Virus Isolate ◽  
Iterative Sequence ◽  
Yellow Mosaic Virus ◽  
Economic Losses ◽  
Polymorphism Analysis ◽  
Southern Mexico ◽  
Bean Plants ◽  
Golden Yellow

In Cuba, the emergence of bean golden mosaic was associated with high populations of Bemisia tabaci in common bean (Phaseolus vulgaris L.) plantings in the 1970s (1). During the last two decades, the disease has caused significant economic losses, forcing some growers to abandon bean production. In Holguín, one of the main bean producing provinces of the country, about 2,000 ha of beans were abandoned in 1991 due to the high incidence of this whitefly-transmitted virus. At that time, yield losses associated with this disease reached 90 to 100% in farmer's fields. In spite of various control measures, the disease affected 33, 28, and 6.5% of the total area planted in Cuba to common bean in 1990, 1992, and 1996, respectively. For this investigation, common bean leaves showing systemic yellowing symptoms were collected in fields located in the provinces of Havana, Matanzas, and Holguín during 1998-1999. Sap and total DNA leaf extracts were used to inoculate healthy bean plants by manual and biolistic procedures, respectively. Characteristic yellowing symptoms were more efficiently reproduced using a particle gun device than by manual inoculation (18/20 plants and 5/20 plants, respectively, for a Holguín virus isolate). DNA extracts were further analyzed by polymerase chain reaction using two degenerate primer sets: PAL1v1978-PAR1c715 and PAL1c1960-PAR1v722 (2). Fragments of approximately 1.4 and 1.2 kb were amplified and cloned. Restriction fragment length polymorphism analysis of the cloned 1.4-kb fragments was performed with BglII, HincII, SalI, EcoRI, PstI, and XbaI, indicating that selected isolates from the three Cuban provinces shared identical restriction patterns. The nucleotide sequence obtained from two clones of a virus isolate from Holguín, was compared to sequences available for other begomoviruses using BLAST. The Cuban isolate shared up to 94% nt sequence identity with various strains of Bean golden yellow mosaic virus (BGYMV) in the first 250 nt of the rep gene. For the common region (CR), scores were 93% for BGYMV-GA (Guatemala), 92% for BGYMV-MX (southern Mexico) and BGYMV-PR (Puerto Rico), and 91% for BGYMV-DR (Dominican Republic). The iterative sequence ATGGAG was identified in the CR of the Cuban BGYMV isolate, as reported for other BGYMV isolates. Finally, the Cuban begomovirus, hereafter referred to as BGYMV-CU, shared nt and aa sequence identities of 94 and 100%, respectively, with the coat protein gene of BGYMV-MX. We conclude that the begomovirus isolated from mosaic-affected common bean plants in the province of Holguín is a member of the Mesoamerican BGYMV group (3). References: (1) N. Blanco and C. Bencomo. Cienc. Agric. 2:39, 1978. (2) M. R. Rojas et al. Plant Dis. 77:340, 1993. (3) Morales and Anderson, Arch. Virol. 146:415, 2001.

scholarly journals Begomoviruses infecting common beans (Phaseolus vulgaris L.) in production areas in Cuba

2018 ◽  
Vol 16 (2) ◽  
pp. e1006
Author(s):  
Lidia Chang-Sidorchuk ◽  
Heidy González-Alvarez ◽  
Yamila Martínez-Zubiaur
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Dna Sequences ◽  
Rolling Circle Amplification ◽  
Yellow Mosaic Virus ◽  
Polymorphism Analysis ◽  
Tobacco Leaf ◽  
Golden Yellow ◽  
Production Areas ◽  
Leaf Curl

Viral diseases caused by begomoviruses are economically important for their depressing impact on common bean production in Cuba. Mayabeque is a Cuban province where this crop is significantly grown and affectations by Bean golden yellow mosaic virus (BGYMV) have been detected in the last 30 years. Integrated pest management (IPM) programs in this crop have allowed controlling the disease for a long time. However, in prospections of the last years, an increase of the incidence of various yellowing symptoms typical of begomoviruses has been observed in common bean production areas. DNA was extracted from leaf samples taken from symptomatic plants. Non-radioactive nucleic acid hybridization and a specific PCR assay were used to detect BGYMV, Common bean severe mosaic virus, Common bean mottle virus, and Tobacco leaf curl Cuba virus. Of the 218 bean plants sampled, 89.5 % was positive to BGYMV; the presence of the rest of the begomovirus species was between 3 and 4% (3.08% of CBMoV, 3.08% of TbLCCuV and 4.32% of CBSMV). The viral DNA from some samples was analyzed by rolling circle amplification (RCA), by restriction fragment length polymorphism analysis using restriction enzymes, and by cloning and sequencing of the viral components. The DNA sequences from BGYMV isolates showed 98% of identity with the isolates reported in Cuba in 2003. The infection by Tobacco leaf curl Cuba virus (TbLCCuV) was confirmed also in fields in the Cuban western region. This is the first work where the DNA-B of TbLCCuV is identified. These studies will help to strengthen phytosanitary surveillance and management programs implemented in the country to control the whitefly-begomovirus complex in this economically important crop.

scholarly journals Two Newly Described Begomoviruses of Macroptilium lathyroides and Common Bean

2003 ◽  
Vol 93 (7) ◽  
pp. 774-783 ◽  
Author(s):  
A. M. Idris ◽  
E. Hiebert ◽  
J. Bird ◽  
J. K. Brown
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Dna Sequences ◽  
Western Hemisphere ◽  
Yellow Mosaic Virus ◽  
Nucleotide Identity ◽  
Caribbean Region ◽  
Golden Yellow ◽  
The Caribbean ◽  
Macroptilium Lathyroides

Macroptilium lathyroides, a perennial weed in the Caribbean region and Central America, is a host of Macroptilium yellow mosaic Florida virus (MaYMFV) and Macroptilium mosaic Puerto Rico virus (MaMPRV). The genomes of MaYMFV and MaMPRV were cloned from M. lathyroides and/or field-infected bean and the DNA sequences were determined. Cloned A and B components for both viruses were infectious when inoculated to M. lathyroides and common bean. Comparison of the DNA sequences for cloned A and B components with well-studied begomovirus indicated that MaMPRV (bean and M. lathyroides) and MaYMFV (M. lathyroides) are unique, previously undescribed begomo-viruses from the Western Hemisphere. Phylogenetic analysis of viral A components indicated that the closest relative of MaYMFV are members of the Bean golden yellow mosaic virus (BGYMV) group, at 76 to 78% nucleotide identity, whereas the closest relative for the A component of MaMPRV was Rhynchosia golden mosaic virus at 78% nucleotide identity. In contrast, BGYMV is the closest relative for the B component of both MaYMFV and MaMPRV, with which they share ≈68.0 and ≈72% identity, respectively. The incongruent taxonomic placement for the bipartite components for MaMPRV indicates that they did not evolve entirely along a common path. MaYMFV and MaMPRV caused distinctive symptoms in bean and M. lathyroides and were transmissible by the whitefly vector and by grafting; however, only MaYMFV was mechanically transmissible. The experimental host range for the two viruses was similar and included species within the families Fabaceae and Malvaceae, but only MaYMFV infected Malva parviflora and soybean. These results collectively indicate that MaMPRV and MaYMFV are new, previously undescribed species of the BGYMV group, a clade previously known to contain only strains and isolates of BGYMV from the Caribbean region that infect Phaseolus spp. Both MaYMFV and MaMPRV may pose an economic threat to bean production in the region.

scholarly journals Begomoviruses Associated with Bean Golden Mosaic Disease in Nicaragua

Plant Disease ◽  
2011 ◽  
Vol 95 (8) ◽  
pp. 901-906 ◽  
Author(s):  
J. Karkashian ◽  
E. D. Ramos-Reynoso ◽  
D. P. Maxwell ◽  
P. Ramírez
Keyword(s):  
Mosaic Virus ◽  
Northern Region ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Bean Plants ◽  
Three Samples ◽  
Geographical Regions ◽  
Golden Yellow ◽  
Virus Sequence ◽  
Viral Sequences

Begomovirus spp. cause substantial losses in bean crops in tropical and subtropical regions of the Americas. The predominant Begomovirus sp. in Central America associated with golden mosaic symptoms in bean is Bean golden yellow mosaic virus (BGYMV). However, Calopogonium golden mosaic virus was previously found to infect bean crops in the northern region of Costa Rica. The objective of this research was to identify Begomovirus spp. that infect bean plants in different geographical regions of Nicaragua. In all, 126 samples of young bean leaves with symptoms of golden mosaic were collected from eight different regions of Nicaragua. Using DNA hybridization with specific probes, 120 samples tested positive for BGYMV, 14 samples tested positive for Squash yellow mild mottle virus, and 7 samples tested positive for Calopogonium golden mosaic virus. Sequence analysis of polymerase chain reaction-amplified products from three samples (MA-9 Managua, BE-8 Rivas, and SO-9 Granada) also indicated that the symptoms of golden mosaic in bean are associated with viral sequences from three different Begomovirus spp. Management of bean golden mosaic disease must take into account that BGYMV is the predominant virus (95% of the samples) and that 12% of the samples exhibited possible mixed infections or recombination events in the south and central geographical regions of Nicaragua.

scholarly journals Two Genes from Phaseolus coccineus Confer Resistance to Bean Golden Yellow Mosaic Virus in Common Bean

2007 ◽  
Vol 132 (4) ◽  
pp. 530-533 ◽  
Author(s):  
Juan M. Osorno ◽  
Carlos G. Muñoz ◽  
James S. Beaver ◽  
Feiko H. Ferwerda ◽  
Mark J. Bassett ◽  
...  
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Interspecific Cross ◽  
Dominant Gene ◽  
Recessive Gene ◽  
Phaseolus Coccineus ◽  
Yellow Mosaic Virus ◽  
Sources Of Resistance ◽  
Sequence Characterized Amplified Region ◽  
Golden Yellow

Bean golden yellow mosaic virus (BGYMV), incited by a whitefly (Bemisia tabaci Gennadius) transmitted geminivirus, is an important disease that can limit common bean (Phaseolus vulgaris L.) production in Central America, the Caribbean, and southern Florida. Only a few genes are currently deployed in BGYMV-resistant common bean cultivars. The identification of novel sources of resistance would help bean breeders broaden the genetic base of resistance to this important virus. Phaseolus coccineus L. germplasm accession G35172 was found by International Center for Tropical Agriculture scientists to be resistant to BGYMV. Populations derived from an interspecific cross between P. vulgaris and P. coccineus were evaluated to study the inheritance of resistance to BGYMV. Segregation ratios of F2 plants and other populations suggest that BGYMV resistance from P. coccineus is controlled by two genes. A recessive gene, with the proposed symbol bgm-3, confers resistance to leaf chlorosis and a dominant gene, with the proposed name Bgp-2, prevents pod deformation in the presence of BGYMV. Results from allelism tests with previously reported BGYMV resistance genes (bgm, bgm-2, and Bgp) and the absence of the SR-2 sequence-characterized amplified region marker for bgm support the hypothesis that bgm-3 and Bgp-2 are different genes for BGYMV resistance.

scholarly journals NAC Candidate Gene Marker for bgm-1 and Interaction With QTL for Resistance to Bean Golden Yellow Mosaic Virus in Common Bean

2021 ◽  
Vol 12 ◽  
Author(s):  
Alvaro Soler-Garzón ◽  
Atena Oladzad ◽  
James Beaver ◽  
Stephen Beebe ◽  
Rian Lee ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Common Bean ◽  
Mosaic Virus ◽  
Genome Wide Association Study ◽  
Stop Codon ◽  
Genetic Resistance ◽  
Yellow Mosaic Virus ◽  
Gene Marker ◽  
Exon 2 ◽  
Golden Yellow

Genetic resistance is the primary means for control of Bean golden yellow mosaic virus (BGYMV) in common bean (Phaseolus vulgaris L.). Breeding for resistance is difficult because of sporadic and uneven infection across field nurseries. We sought to facilitate breeding for BGYMV resistance by improving marker-assisted selection (MAS) for the recessive bgm-1 gene and identifying and developing MAS for quantitative trait loci (QTL) conditioning resistance. Genetic linkage mapping in two recombinant inbred line populations and genome-wide association study (GWAS) in a large breeding population and two diversity panels revealed a candidate gene for bgm-1 and three QTL BGY4.1, BGY7.1, and BGY8.1 on independent chromosomes. A mutation (5 bp deletion) in a NAC (No Apical Meristem) domain transcriptional regulator superfamily protein gene Phvul.003G027100 on chromosome Pv03 corresponded with the recessive bgm-1 resistance allele. The five bp deletion in exon 2 starting at 20 bp (Pv03: 2,601,582) is expected to cause a stop codon at codon 23 (Pv03: 2,601,625), disrupting further translation of the gene. A Tm-shift assay marker named PvNAC1 was developed to track bgm-1. PvNAC1 corresponded with bgm-1 across ∼1,000 lines which trace bgm-1 back to a single landrace “Garrapato” from Mexico. BGY8.1 has no effect on its own but exhibited a major effect when combined with bgm-1. BGY4.1 and BGY7.1 acted additively, and they enhanced the level of resistance when combined with bgm-1. Tm-shift assay markers were generated for MAS of the QTL, but their effectiveness requires further validation.

scholarly journals Screening Metagenomic Data for Viruses Using the E-Probe Diagnostic Nucleic Acid Assay

2014 ◽  
Vol 104 (10) ◽  
pp. 1125-1129 ◽  
Author(s):  
A. H. Stobbe ◽  
W. L. Schneider ◽  
P. R. Hoyt ◽  
U. Melcher
Keyword(s):  
Nucleic Acid ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Metagenomic Data ◽  
Data Sets ◽  
Virus Species ◽  
Data Set ◽  
Golden Yellow ◽  
Nucleic Acid Assay ◽  
Ngs Data

Next generation sequencing (NGS) is not used commonly in diagnostics, in part due to the large amount of time and computational power needed to identify the taxonomic origin of each sequence in a NGS data set. By using the unassembled NGS data sets as the target for searches, pathogen-specific sequences, termed e-probes, could be used as queries to enable detection of specific viruses or organisms in plant sample metagenomes. This method, designated e-probe diagnostic nucleic acid assay, first tested with mock sequence databases, was tested with NGS data sets generated from plants infected with a DNA (Bean golden yellow mosaic virus, BGYMV) or an RNA (Plum pox virus, PPV) virus. In addition, the ability to detect and differentiate among strains of a single virus species, PPV, was examined by using probe sets that were specific to strains. The use of probe sets for multiple viruses determined that one sample was dually infected with BGYMV and Bean golden mosaic virus.

scholarly journals Detecting bean golden yellow mosaic virus in bean breeding lines and in the common legume weed Macroptilium lathyroides in Puerto Rico.

1969 ◽  
Vol 85 (3-4) ◽  
pp. 165-176
Author(s):  
Lydia I. Rivera-Vargas ◽  
Vilmaris Bracero-Acosta ◽  
James S. Beaver ◽  
Dan E. Purcifull ◽  
Jane E. Polston ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Phaseolus Vulgaris L ◽  
Breeding Lines ◽  
Golden Yellow ◽  
Enzymelinked Immunosorbent Assay ◽  
The Common ◽  
Polymerase Chain ◽  
Macroptilium Lathyroides

Bean golden yellow mosaic virus (BGYMV) is a geminivirus transmitted by whiteflies (Genus: Bemisia). This virus causes significant fosses in common bean (Phaseolus vulgaris L.). Serological techniques such as enzymelinked immunosorbent assay (ELISA) have been widely used for detection of viruses. We evaluated existing monoclonal antibodies (3F7,2G5 and 5C5) for the detection of BGYMV isolates in bean fines in Puerto Rico. Monoclonal antibody 3F7 was the most effective in detecting the virus in tissues of line DOR 364 and susceptible cuftivars Top Crop and Quest. However, it was not effective in the detection of BGYMV in lines of DOR 303, which showed typical symptoms. Sampfes from Macroptilium lathyroides, a weed that might be a possible reservoir of the virus, were also tested for viraf infection. ELISA tests were inconclusive for detection of geminiviruses in M. lathyroides. Polymerase Chain Reaction (PCR) was also used to complement BGYMV diagnosis in M. lathyroides and in bean lines that showed symptoms but were negative for the ELfSA test. Two sets of primers, specific for Begomovirus such as BGYMV, were used in PCR experiments. Using PCR, we were able to detect the virus in the line DOR 303 and in M. lathyroides tissues.

scholarly journals Mosca-Branca, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) em feijoeiro: Características gerais, bioecologia e métodos de controle

2017 ◽  
Vol 10 (1) ◽  
pp. 01-08
Author(s):  
Anderson Gonçalves da Silva ◽  
Arlindo Leal Boiça Junior ◽  
Bruno Henrique Sardinha de Souza ◽  
Eduardo Neves Costa ◽  
James da Silva Hoelhert ◽  
...  
Keyword(s):  
Common Bean ◽  
Bemisia Tabaci ◽  
Crop Production ◽  
Insect Pests ◽  
Economic Losses ◽  
Common Beans ◽  
Bean Plants ◽  
De Se ◽  
Before And After ◽  
The Common

Resumo. A cultura do feijoeiro pode ser infestada por insetos que afetam a produção antes e após a colheita, tendo como estimativa de perdas causadas nos rendimentos pelas pragas variando de 33 a 86%. Dentre essas pragas a mosca-branca Bemisia tabaci (Genn.) Autor merece destaque. Esta ocasiona danos diretos decorrentes de sua alimentação e indiretos que ocorrem por meio da excreção açucarada de honeydew ou “mela” e simbiose com a fumagina. No entanto, o dano mais sério causado pela B. tabaci é a transmissão de viroses como o mosaico-dourado-do-feijoeiro, provocando perdas econômicas que podem variar de 30% a 100%. Desse modo, o objetivo do presente estudo é disponibilizar informações a respeito de aspectos importantes de B. tabaci, como: histórico e distribuição geográfica, bioecologia e dinâmica populacional, plantas hospedeiras, métodos de controle adotados, dentre outros, a fim de se fornecer subsídios para futuras pesquisas sobre a mosca-branca em feijão.Whitefly Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) in common beans: General characteristics, bioecology, and methods of controlAbstract. Common bean plants are infested by insects, which can ultimately affect the crop production before and after harvest, with estimated losses ranging from 33 to 86%. Among the insect pests infesting the common beans the whitefly Bemisia tabaci (Genn.) stands out. This species cause direct injury by feeding on the plants and indirect injury by excreting sugary honeydew that is after colonized by the sooty mold. In addition, the most serious damage caused by B. tabaci is the transmission of virus diseases, especially the common bean golden mosaic, responsible for economic losses varying from 30 to 100%. This review aims at providing information on important aspects of B. tabaci including its geographical distribution, bioecology, population dynamics, host plants, and methods of pest control. We expect that this review can provide valuable subsidies for future studies on the whitefly in common beans.

Genomic sequence variability of an Italian Zucchini yellow mosaic virus isolate

2019 ◽  
Vol 156 (1) ◽  
pp. 325-332
Author(s):  
Giovanni Bubici ◽  
Beatriz Navarro ◽  
Anna Vittoria Carluccio ◽  
Marina Ciuffo ◽  
Francesco Di Serio ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Isolate ◽  
Genomic Sequence ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Sequence Variability
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