scholarly journals First Report on the Occurrence of Grapevine leafroll-associated virus 5 in Chilean Grapevines

Plant Disease ◽  
2010 ◽  
Vol 94 (8) ◽  
pp. 1067-1067 ◽  
Author(s):  
E. A. Engel ◽  
P. F. Escobar ◽  
P. A. Rivera ◽  
P. D. T. Valenzuela
Keyword(s):  
Amino Acid ◽  
Protein Gene ◽  
Amino Acid Identity ◽  
Specific Primers ◽  
Sauvignon Blanc ◽  
Reverse Transcription Pcr ◽  
Reference Isolate ◽  
Grapevine Virus A ◽  
Rupestris Stem Pitting ◽  
Stem Pitting

Grapevine leafroll is one of the most widespread and economically damaging viral diseases of grapevines. At least eight distinct Grapevine leafroll-associated viruses (GLRaVs), all members of the Closteroviridae family, have been associated with this disease (4). GLRaV-5 was recently reported in vineyards from Argentina (2). To determine if GLRaV-5 was present in Chilean grapevines, in addition to the previously reported GLRaV-1, -2, -3, -4, -7, and -9 (1), 45 dormant cane samples from 12 different cultivars were collected from different geographic regions of Chile and screened by reverse transcription-PCR. Two of the forty-five samples (cvs. Sauvignon Blanc and Superior) collected from the III (700 km north of Santiago) and VI (150 km south of Santiago) regions of Chile, respectively, were found to be infected with GLRaV-5 using two different pairs of virus-specific primers. The first pair of primers, LR5-1F: 5′-CCCGTGATACAAGGTAGGACA-3′ and LR5-1R: 5′-CAGACTTCACCTCCTGTTAC-3′ (3), was used to amplify a 690-bp fragment corresponding to a partial region of the coat protein gene. The sequences obtained from the two positive samples (GenBank Accession Nos. HM214148 and HM214149) shared 97 and 94% of nucleotide identities, respectively, with the corresponding fragment of a reference GLRaV-5 isolate (GenBank Accession No. EU815935). Both samples shared 99% of amino acid identity with the same reference isolate. A second pair of primers, LR5upF: 5′-CTCTGCTTTTCTGCTGGCA-3′ and LR5doR: 5′-TATCTTTTATCTCCCGATAAACGAG-3′ (4) that amplified a 160-bp fragment of the HSP70h gene was also used. The positive Chilean samples (GenBank Accession Nos. HM214150 and HM214151) shared in both cases 98% nucleotide and 98% amino acid identities with the corresponding fragment of a reference GLRaV-5 isolate (Accession No. AF039552). The two GLRaV-5-positive plants were additionally infected with other viruses previously reported in Chile (1). The cv. Sauvignon Blanc sample was also infected with GLRaV-2, Grapevine fleck virus, and Grapevine rupestris stem pitting-associated virus. The cv. Superior sample was also infected with GLRaV-3, GLRaV-4, and Grapevine virus A. References: (1) E. A. Engel et al. J. Virol. Methods 163:445, 2010. (2) S. Gomez et al. Virus Genes 38:184, 2009. (3) X. Good and J. Monis. Phytopathology 91:274, 2001. (4) V. I. Maliogka et al. J. Virol. Methods 154:41, 2008.

scholarly journals First Report of Grapevine Syrah virus-1 in Chilean Grapevines

Plant Disease ◽  
2010 ◽  
Vol 94 (5) ◽  
pp. 633-633 ◽  
Author(s):  
E. A. Engel ◽  
P. A. Rivera ◽  
P. D. T. Valenzuela
Keyword(s):  
Amino Acid ◽  
Positive Sample ◽  
Economic Damage ◽  
First Report ◽  
Reverse Transcription Pcr ◽  
Putative Coat Protein ◽  
Methyltransferase Gene ◽  
Rupestris Stem Pitting ◽  
Commercial Antibodies ◽  
Stem Pitting

At least 58 viruses have been reported to infect grapevines, causing economic damage globally. Our lab has reported previously the presence of more than 10 viral species in Chilean grapevines (2,3). Grapevine Syrah virus-1 (GSyV-1) is a novel marafivirus recently described in California vineyards (1). Grapevine virus Q (GVQ) was described shortly after GSyV-1 and both genomes share more than 99% nucleotide identity (4). Since GSyV-1 and GVQ correspond to the same viral species, the name GSyV-1 will be used in the current note to avoid confusion. Forty dormant cane samples from 12 different cultivars were collected from different regions of Chile and screened by reverse transcription-PCR. One of the 40 samples (cv. Syrah) collected from the VI region of Chile was found to be infected with GSyV-1 using two different pairs of GSyV-1-specific primers. The first pair of primers GSyV-1Det-F: 5′-CAAGCCATCCGTGCATCTGG -3′ and GSyV-1Det-R: 5′-GCCGATTTGGAACCCGATGG -3′ (1), was used to amplify a 297-bp fragment corresponding to a partial region of the putative methyltransferase gene. The sequence (GenBank Accession No. GU566025) shared 87% nucleotide and 100% amino acid identities with the corresponding fragment of a Californian GSyV-1 isolate (GenBank Accession No. FJ436028). Since there are no commercial antibodies available for GSyV-1 detection, a second pair of primers, GVQCP-F: 5′-TCCCAGCTTCAGGGTGAATT -3′ and GVQCP-R: 5′-GCATTGCTGCGCATTGGAGG -3′ (4), that amplified a 720-bp fragment of the putative coat protein gene was also used. The sequence of 720 bp from the Chilean sample (GenBank Accession No. GU566024) shared 92% nucleotide and 98% amino acid identities with the corresponding fragment of a Californian GSyV-1 isolate (GenBank Accession No. FJ436028). The GSyV-1-positive sample was also infected with Grapevine fleck virus and Grapevine rupestris stem pitting-associated virus that have been reported previously in Chile. To our knowledge, this is the first report of GSyV-1 in Chile. Further studies will help to establish the incidence and effects of this virus in Chilean grapevines. References: (1) M. Al Rwahnih et al. Virology 387:395, 2009. (2) E. Engel et al. J. Virol. Methods. 163:445, 2010. (3) P. F. Escobar et al. Plant Dis. 92:1474, 2008. (4) S. Sabanadzovic et al. Virology 394:1, 2009.

scholarly journals Incidência de vírus em videiras no Nordeste brasileiro e caracterização molecular parcial de isolados virais locais

2015 ◽  
Vol 45 (3) ◽  
pp. 379-385 ◽  
Author(s):  
Aricléia de Moraes Catarino ◽  
Thor Vinícius Martins Fajardo ◽  
Gilvan Pio-Ribeiro ◽  
Marcelo Eiras ◽  
Osmar Nickel
Keyword(s):  
Grapevine Virus ◽  
Rt Pcr ◽  
Grapevine Fanleaf Virus ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Rupestris Stem Pitting ◽  
Stem Pitting

Os objetivos deste trabalho foram identificar as espécies virais presentes em vinhedos comerciais de duas regiões do Nordeste do Brasil e realizar a caracterização molecular parcial de isolados de três espécies virais. A diagnose foi realizada por meio de RT-PCR em tempo real para a detecção de Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine leafroll-associated virus 2, 3 e 4 (GLRaV-2, -3 e -4), Grapevine fleck virus (GFkV), Grapevine rupestris vein feathering virus (GRVFV) e Grapevine fanleaf virus (GFLV). Exceto para GFLV, os vírus avaliados estão amplamente disseminados nas áreas amostradas, frequentemente em altas incidências e em infecções múltiplas, de até 98% e 76,4%, na Zona da Mata e no Vale do São Francisco, respectivamente. Isolados locais de GVA, GVB e GLRaV-3 foram parcialmente caracterizados com base na sequência completa de nucleotídeos do gene da proteína capsidial e apresentaram alta porcentagem de identidade de nucleotídeos com outros isolados brasileiros: 91,2% (GVA), 99,8% (GVB) e 99,7% (GLRaV-3)

scholarly journals First Report on the Occurrence of Grapevine leafroll-associated virus 7 and 9 in Chilean Grapevines

Plant Disease ◽  
2008 ◽  
Vol 92 (8) ◽  
pp. 1252-1252 ◽  
Author(s):  
E. A. Engel ◽  
P. Escobar ◽  
C. Montt ◽  
S. Gómez-Talquenca ◽  
P. D. T. Valenzuela
Keyword(s):  
Amino Acid ◽  
Mixed Infection ◽  
Amino Acid Identity ◽  
Microarray Hybridization ◽  
Rt Pcr ◽  
Specific Primers ◽  
Hybridization Pattern ◽  
Acid Identity ◽  
Pcr Product ◽  
Plant Pathol

Grapevine is one of the oldest horticultural crops and represents a highly valuable agricultural commodity. So far, nine distinct Grapevine leafroll-associated viruses (GLRaVs) within the Closteroviridae family have been found to be associated with grapevine leafroll disease (3). Previous studies have demonstrated a high incidence of GLRaV-1, -2, and -3 in Chile (2). To determine if other GLRaVs were present, 21 dormant cane samples were screened with a comprehensive 70-mer oligonucleotide microarray designed to simultaneously detect all grapevine viruses with total or partial genomic sequence available. The array contained 570 unique probes designed against specific regions of more than 40 viral genomes (E. Engel et al., 15th ICVG [Abstr.], 2006). One sample (cv. Black Seedless) showing a microarray hybridization pattern compatible with a mixed infection of GLRaV-7 and GLRaV-1 was analyzed by ELISA using GLRaV-7 specific antibodies (Agritest, Valenzano, Italy) and reverse transcription (RT)-PCR using virus-specific primers LR7-F: 5′- TAT ATC CCA ACG GAG ATG GC -3′ and LR7-R: 5′- ATG TTC CTC CAC CAA AAT CG -3′ (based on GenBank Accession No. Y15987). The serological analysis confirmed the presence of GLRaV-7 with further confirmation by the RT-PCR product of 502 bp corresponding to a fragment of the HSP70h gene that was cloned and sequenced. The Chilean GLRaV-7 sequence (GenBank Accession No. EU334662) showed 94% nucleotide and 95% amino acid identity when compared with a corresponding region of another GLRaV-7 isolate from Albania (GenBank Accession No. Y15987). GLRaV-1 infection was confirmed by ELISA (Bioreba AG, Reinach, Switzerland) and RT-PCR. A second sample (cv. Tintorera) showing microarray hybridization pattern compatible with a mixed infection of GLRaV-9 and Grapevine virus A (GVA) was analyzed by RT-PCR using virus-specific primers LR9-F: 5′- CGG CAT AAG AAA AGA TGG CAC -3′ and LR9-R: 5′- TCA TTC ACC ACT GCT TGA AC -3′ (1). The RT-PCR product of 393 bp corresponding to a fragment of the HSP70h gene was cloned and sequenced (GenBank Accession No. EU334663), showing 94% nucleotide and 95% amino acid identity when compared with a corresponding region of another GLRaV-9 isolate from the United States (GenBank Accession No. AY297819). Since there are no commercial antibodies available for GLRaV-9 detection, a second pair of primers, LR9-F1: 5′- AAA GGT TTC TGC TGG TTA CC -3′ and LR9-R1: 5′- CTT TCA GAA CAG TCC TCC TC -3′ that amplified a fragment of ORF1a was also used. The 301-bp product was cloned and sequenced (GenBank Accession No. EU588989) showing 93.7% nucleotide and 98% amino acid identity when compared with a corresponding region of another GLRaV-9 isolate (GenBank Accession No. AY297819). GVA infection was confirmed by ELISA (Bioreba AG) and RT-PCR. To our knowledge, this is the first report of GLRaV-7 and GLRaV-9 in Chile. Further studies will help determine the effect and incidence of these viruses in Chilean grapevines. References: (1) R. Alkowni et al. J. Plant Pathol. 86:123, 2004. (2) N. Fiore et al. J. Plant Pathol. 90:125, 2008. (3) G. P. Martelli and E. Boudon-Padieu. Options Méditerr. B55, 2006.

scholarly journals Desempenho agronômico de videiras com e sem sintomas de viroses, e comparação molecular de isolados virais

2015 ◽  
Vol 50 (7) ◽  
pp. 541-550 ◽  
Author(s):  
Monique Bezerra Nascimento ◽  
Thor Vinícius Martins Fajardo ◽  
Marcelo Eiras ◽  
Ana Beatriz Costa Czermainski ◽  
Osmar Nickel ◽  
...  
Keyword(s):  
Grapevine Virus ◽  
Rt Pcr ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Rupestris Stem Pitting ◽  
Stem Pitting

Resumo: O objetivo deste trabalho foi avaliar os efeitos de viroses em videiras sintomáticas e assintomáticas sobre as variáveis agronômicas relacionadas ao vigor das plantas e à qualidade enológica da uva, e comparar os isolados virais obtidos nessas duas condições. Realizaram-se dois experimentos com quatro cultivares. Todas as plantas foram indexadas, por meio da reação em cadeia da polimerase via transcrição reversa (RT-PCR) em tempo real, quanto à provável ocorrência dos seguintes vírus: Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine leafroll-associated virus (GLRaV-1 ao -4, GLRaV-4 estirpe 5), Grapevine rupestris stem pitting-associated virus (GRSPaV) e Grapevine fleck virus (GFkV). As variáveis avaliadas foram: número de gemas brotadas e não brotadas, número de ramos com ou sem cachos, número total de gemas, número de cachos, massa de cachos frescos, massa total de bagas, massa do engaço, número de bagas por cacho, massa média de baga, sólidos solúveis totais, acidez total titulável, pH, massa de ramos podados ou diâmetros do tronco do porta-enxerto e da copa. Os efeitos negativos foram mais pronunciados nas plantas com sintomas de viroses; no entanto, constatou-se frequentemente que plantas sem sintomas também estavam infectadas. A análise molecular de GRSPaV, GVA e GLRaV-2, isolados de plantas sintomáticas e assintomáticas, resultou em alta percentagem de identidade de nucleotídeos entre isolados homólogos.

scholarly journals First Detection of Rupestris stem pitting associated virus Particles by Antibody to a Recombinant Coat Protein

Plant Disease ◽  
2003 ◽  
Vol 87 (5) ◽  
pp. 510-514 ◽  
Author(s):  
Natasa Petrovic ◽  
Baozhong Meng ◽  
Maja Ravnikar ◽  
Irena Mavric ◽  
Dennis Gonsalves
Keyword(s):  
Coat Protein ◽  
Polyclonal Antiserum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Grapevine Virus ◽  
Virus Particles ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Rupestris Stem Pitting ◽  
Recombinant Coat Protein ◽  
Stem Pitting

Rupestris stem pitting associated virus (RSPaV), a member of the genus Foveavirus, is associated with the Rupestris stem pitting component of the Rugose wood (RW) disease complex of grapevines. Heretofore, particles of RSPaV have not been visualized. In this work, flexuous rod particles approximately 723 nm in length were detected in the sap of infected grapevines by immunosorbent electron microscopy (ISEM), using a polyclonal antiserum produced to a recombinant coat protein of RSPaV. Particles of RSPaV were detected in tissue culture-, greenhouse-, and field-grown grapevines infected with RSPaV, but not in healthy control plants. Detection of virus particles by ISEM corresponded with detection of RSPaV by Western blot, enzyme-linked immunosorbent assay, and reverse transcription-polymerase chain reaction. Virus particles were decorated with the antibodies specific to RSPaV but not with antibodies to Grapevine virus A or Grapevine virus B, two other viruses believed to be associated with RW. This definitive identification of RSPaV particles will help define the etiology of RW.

scholarly journals First Detection of Grapevine leafroll-associated virus 4 in Chilean Grapevines

Plant Disease ◽  
2008 ◽  
Vol 92 (10) ◽  
pp. 1474-1474 ◽  
Author(s):  
P. F. Escobar ◽  
N. Fiore ◽  
P. D. T. Valenzuela ◽  
E. A. Engel
Keyword(s):  
Coat Protein ◽  
Protein Gene ◽  
Nucleotide Identity ◽  
Viral Diseases ◽  
Thompson Seedless ◽  
Specific Primers ◽  
Partial Region ◽  
Reverse Transcription Pcr ◽  
Plant Pathol ◽  
Commercial Antibodies

Grapevine leafroll is one of the most widespread and economically relevant viral diseases of grapevines. At least nine distinct Grapevine leafroll-associated viruses (GLRaVs), all members of the Closteroviridae family, have been associated with this disease in grapevine. Grapevine leafroll-associated virus 4 (GLRaV-4), currently classified as a Closteroviridae member under the Ampelovirus genus, was initially described in California. To determine if GLRaV-4 was present in Chilean grapevines, in addition to the previously reported GLRaV-1, -2, -3, -7, and -9 (1,2), 35 dormant cane samples from 12 different cultivars were collected from different regions of Chile and screened by reverse transcription-PCR. Two of the 35 samples (both cv. Thompson Seedless) collected from the III and VI regions of Chile were found to be infected with GLRaV-4 using two different pairs of GLRaV-4 specific primers. The first pair of primers, HSPV-F: 5′- ACA TTC TCC ACC TTG TGC TTT T -3′ and HSPC-R: 5′- CAT ACA AGC GAG TGC AAT TAC -3′ (3), was used to amplify a 321-bp fragment corresponding to a partial region of the HSP70h gene. The sequence (GenBank Accession Nos. EU746618 and EU746619) from both positive samples shared 98.4% nucleotide identity and approximately 99% identity with the corresponding fragment of a Californian GLRaV-4 isolate (GenBank Accession No. AF039553). Since there are no commercial antibodies available for GLRaV-4 detection, a second pair of primers, LR4CPINT-F: 5′- GAG AGT GAC AAG CAC CAG GTG C -3′ and LR4CPFIN-R: 5′- TCA CCT CCT GTT GCC CA -3′ (4), that amplified a 492-bp fragment of the coat protein gene was also used. The sequences of the 492-bp fragment from both Chilean samples (GenBank Accession Nos. EU746620 and EU746621) shared 99.6% nucleotide identity with one another and had 96.5% identity with an Israeli GLRaV-4 isolate (GenBank Accession No. AM176759). To our knowledge, this is the first report of GLRaV-4 in Chile. Further studies will help to establish the effects and incidence of this virus in Chilean grapevines. References: (1) E. Engel et al. Plant Dis. 92:1252, 2008 (2) N. Fiore et al. J. Plant Pathol. 90:125, 2008. (3) F. Osman et al. J. Virol. Methods 141:22, 2007. (4) P. Saldarelli et al. J. Plant Pathol. 88:203, 2006.

scholarly journals A Survey of Virginia Vineyards Revealed High Incidences of Grapevine Rupestris Stem Pitting-Associated Virus, Grapevine Red Blotch Virus, and Two Mealybug Species

2019 ◽  
Vol 20 (4) ◽  
pp. 207-214 ◽  
Author(s):  
Taylor Jones ◽  
Mizuho Nita
Keyword(s):  
Virus Disease ◽  
Grapevine Virus ◽  
Factors Affecting ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Grapevine Pinot Gris Virus ◽  
Potential Factors ◽  
Pseudococcus Viburni ◽  
Rupestris Stem Pitting ◽  
Stem Pitting

We investigated the prevalence of viruses infecting grapevines in Virginia, identity of disease vectors, and potential factors affecting virus incidence. Tested viruses were grapevine leafroll-associated virus (GLRaV-1 and -4), grapevine fleck virus (GFkV), grapevine virus A (GVA), grapevine virus B (GVB), grapevine rupestris stem pitting-associated virus (GRSPaV), tomato ringspot virus (ToRSV), grapevine vein clearing virus (GVCV), grapevine red blotch virus (GRBV), and grapevine Pinot gris virus (GPGV). We documented wide distributions of GRSPaV (54%) and GRBV (24%) and common occurrences of grape (Pseudococcus maritimus) and Gill’s (Ferrisia gilli) mealybugs among vineyards. This is the first report of GLRaV-1, GLRaV-4, GVA, GVB, GRSPaV, and obscure mealybug (Pseudococcus viburni) in Virginia. We also documented significant association (P ≤ 0.05) of the presence of mealybugs and GVA and GVB. With younger vines, significantly lower incidences were found for viruses that were listed (i.e., tested for a certification) by the Foundation Planting Service’s and the National Clean Plant Network’s grape programs. On the other hand, there was a lack of the age effect on incidence of GRSPaV and GRBV, which were not listed until recently. These results suggest the importance of clean plant material and vector management for grapevine virus disease management in Virginia.

scholarly journals Genetic Variability among ampC Genes from Acinetobacter Genomic Species 3

2008 ◽  
Vol 53 (3) ◽  
pp. 1177-1184 ◽  
Author(s):  
Alejandro Beceiro ◽  
Astrid Pérez ◽  
Felipe Fernández-Cuenca ◽  
Luis Martínez-Martínez ◽  
Alvaro Pascual ◽  
...  
Keyword(s):  
Amino Acid ◽  
Protein Sequences ◽  
Amino Acid Identity ◽  
Clinical Isolates ◽  
Pcr Analysis ◽  
Nucleotide Sequencing ◽  
Moderate Degree ◽  
E Coli ◽  
Reverse Transcription Pcr ◽  
Genomic Species

ABSTRACT As a part of a nationwide study in Spain, 15 clinical isolates of Acinetobacter genomic species 3 (AG3) were analyzed. The main objective of the study was to characterize the ampC genes from these isolates and to determine their involvement in β-lactam resistance in AG3. The 15 AG3 isolates showed different profiles of resistance to ampicillin (range of MICs, 12 to >256 μg/ml). Nucleotide sequencing of the 15 ampC genes yielded 12 new AmpC enzymes (ADC-12 to ADC-23). The 12 AG3 enzymes showed 93.7 to 96.1% amino acid identity with respect to the AmpC enzyme from Acinetobacter baumannii (ADC-1 enzyme). Eight out of fifteen ampC genes were expressed in Escherichia coli cells under the control of a common promoter, and with the exception of one isolate (isolate 65, which showed lower β-lactam MICs), significant differences in overall β-lactam MICs for E. coli cells expressing AG3 ampC genes were not revealed. No significant differences in ampC gene expression in AG3 clinical isolates were revealed by reverse transcription-PCR analysis. A detailed analysis of the 12 AmpC protein sequences revealed that amino acid replacements (in comparison with those of ADC-1) occurred mainly in the same positions, although none were located in important functional domains such as the Ω- loop or conserved β-lactamase motifs. Kinetic experiments performed with three representative AmpC enzymes (ADC-14, -16, and -18) in some cases revealed dramatic changes in Km and k cat values for β-lactams. No ISAba1 was detected upstream of the ampC genes. Our results reveal 12 new ampC genes in AG3. The enzymes showed a moderate degree of variability, and they are tentatively named ADC-12 to ADC-23.

scholarly journals Viral Diversity in Autochthonous Croatian Grapevine Cultivars

Plant Disease ◽  
2017 ◽  
Vol 101 (7) ◽  
pp. 1230-1235 ◽  
Author(s):  
Darko Vončina ◽  
Maher Al Rwahnih ◽  
Adib Rowhani ◽  
Mona Gouran ◽  
Rodrigo P. P. Almeida
Keyword(s):  
Coastal Region ◽  
Germplasm Collection ◽  
Grapevine Virus A ◽  
Group I ◽  
High Infection ◽  
Grapevine Pinot Gris Virus ◽  
Leaf Deformation ◽  
Rupestris Stem Pitting ◽  
First Time ◽  
Stem Pitting

A survey was conducted on nine autochthonous grapevine cultivars grown along the Croatian coastal region. In total, 48 vines (44 from germplasm collection, 4 from vineyards) originating from 23 sites were tested for 26 viruses using molecular methods. Results revealed high infection rates with Grapevine leafroll-associated virus 3 (GLRaV-3); Grapevine virus A (GVA, both 91.7%); Grapevine fleck virus (GFkV, 87.5%); and Grapevine rupestris stem pitting-associated virus (GRSPaV, 83.3%). Other detected viruses were: Grapevine fanleaf virus (GFLV); Grapevine leafroll-associated viruses 1, 2, and strains of 4 (GLRaV-1, GLRaV-2, GLRaV-4); Grapevine viruses B, D, F (GVB, GVD, GVF); Grapevine red globe virus (GRGV); Grapevine vein feathering virus (GVFV); Grapevine Syrah virus 1 (GSyV-1); and Grapevine Pinot gris virus (GPGV). No virus-free vine was found. Mixed infections were determined in all vines, the number of viruses in a single vine ranged from three to nine. GLRaV-3 variant typing confirmed presence of group I, II, and III. Four vines with leaf deformation and mottling were positive for GPGV. Seven viruses (GLRaV-4-like group, GVD, GVE, GVF, GRGV, GSyV-1, and GVFV) were detected for the first time in Croatia. This survey confirmed the deteriorated sanitary status of autochthonous Croatian grapevine cultivars.

Sign in / Sign up

Export Citation Format

Share Document