scholarly journals Incidence of Tomato spotted wilt virus (Bunyaviridae) and Tobacco Thrips in Virginia-Type Peanuts in North Carolina

Plant Disease ◽  
2000 ◽  
Vol 84 (4) ◽  
pp. 459-464 ◽  
Author(s):  
L. E. Garcia ◽  
R. L. Brandenburg ◽  
J. E. Bailey
Keyword(s):  
North Carolina ◽  
Tomato Spotted Wilt Virus ◽  
Disease Incidence ◽  
Block Design ◽  
Virus Titer ◽  
Field Trials ◽  
Preliminary Evaluation ◽  
Tomato Spotted Wilt ◽  
Tobacco Thrips ◽  
Wilt Virus

Virginia-type peanut (Arachis hypogaea) cultivars were monitored for incidence of Tomato spotted wilt virus (TSWV) and abundance of Frankliniella fusca, the tobacco thrips, in North Carolina during 1995 and 1996. A preliminary evaluation of 225 peanut genotypes for TSWV-resistant or -tolerant genotypes was conducted in 1995. The incidence of TSWV in cultivar NC-9 was twice that of cultivar NC-V11. In 1996, field trials designed to evaluate TSWV susceptibility were conducted with three widely grown commercial peanut cultivars in North Carolina. They were NC-9, NC-V11, and NC-12C, a newly released cultivar. A randomized complete block design was utilized at three locations. Disease incidence was evaluated weekly from 2 weeks postplanting until 2 weeks prior to harvest. Mechanical inoculation of the three cultivars resulted in no difference in relative leaf virus titer as determined from optical density readings following DAS-ELISA for 4 successive weeks beginning at 13 days postinoculation. NC-9 ranked highest in incidence of disease (7%), followed by NC-12C (6%) and NC-V11 (5%). Thrips counts were greatest on NC-V11, followed by NC-9 and NC-12C. Disease incidence overall was 5.96%, but ranged from 3.08 to 11.15% among the three sites. Yield was affected by the temporal occurrence of symptoms beginning at the fifth week postplanting. Greatest yield losses occurred in those plants with the earliest visible foliar symptoms.

scholarly journals Survival and Reproductive Success of Tobacco Thrips on Three Tomato Spotted Wilt Virus Infected and Noninfected Peanut Cultivars

2000 ◽  
Vol 27 (2) ◽  
pp. 49-52 ◽  
Author(s):  
L. E. Garcia ◽  
G. G. Kennedy ◽  
R. L. Brandenburg
Keyword(s):  
North Carolina ◽  
Reproductive Success ◽  
Tomato Spotted Wilt Virus ◽  
Mixed Model ◽  
Mixed Model Analysis ◽  
Tomato Spotted Wilt ◽  
Frankliniella Fusca ◽  
Tobacco Thrips ◽  
Peanut Cultivars ◽  
Wilt Virus

Abstract A comparison of the survival and reproductive success of Frankliniella fusca (Hinds) (Thysanoptera: Thripidae) on tomato spotted wilt virus (Bunyviridae: Tospovirus) (TSWV) infected and uninfected peanut plants was conducted under greenhouse conditions in North Carolina. Three cultivars—NC 9, NC-V11, and NC 12C—adapted to North Carolina production practices were evaluated. A total of 180 individually caged plants, in three replicates, were infested with 20 female F. fusca each. Adult and larval thrips were collected after 30 d on the plants. Final counts were square root transformed and a mixed model analysis of variance conducted. Effects of cultivar and the virus-by-cultivar interaction were not statistically significant. TSWV-infected plants had significantly fewer adult and larval F. fusca than did uninfected plants for adults (P = 0.04) and for larvae (P = 0.01). This study reports on an alternative method of assessing TSWV resistance among peanut cultivars and the trend appears to support the conclusions of a previous field study, which found NC 9 more susceptible to TSWV than either NC-V11 or NC 12C.

scholarly journals Management of Tomato spotted wilt virus in Flue-Cured Tobacco with Acibenzolar-S-Methyl and Imidacloprid

Plant Disease ◽  
2001 ◽  
Vol 85 (3) ◽  
pp. 292-296 ◽  
Author(s):  
A. S. Csinos ◽  
H. R. Pappu ◽  
R. M. McPherson ◽  
M. G. Stephenson
Keyword(s):  
Field Trial ◽  
Tomato Spotted Wilt Virus ◽  
Disease Incidence ◽  
Field Trials ◽  
Growth And Yield ◽  
Yield Potential ◽  
Phytophthora Parasitica ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Effect On Growth

Tomato spotted wilt virus (TSWV) continues to be a major production constraint in flue-cured tobacco in Georgia. Pretransplant applications of acibenzolar-S-methyl (Actigard) and imidacloprid (Admire and Provado) were evaluated in field trials, singly and in combination, in four locations in 1999. Acibenzolar-S-methyl was also evaluated for its effect on growth and yield, potential phytotoxicity, and activity against tobacco blackshank incited by Phytophthora parasitica var. nicotianae. Acibenzolar-S-methyl alone and with imidacloprid significantly reduced TSWV incidence in all four locations, but the disease incidence in the imidacloprid-treated plots was lower in only one of the four sites. Applications of acibenzolar-S-methyl starting at 4 weeks posttransplant had no effect on TSWV incidence, size, or yield of tobacco compared with nontreated plots. Acibenzolar-S-methyl did not reduce blackshank in a field trial. In the greenhouse, rates of acibenzolar-S-methyl ranging from 0.25 to 8 g a.i. per 7,000 plants showed reductions in growth that did not seem to be related to rate. Pretransplant applications of acibenzolar-S-methyl were critical to the suppression of TSWV, while initial applications made posttransplant had no effect, suggesting that plants must be protected prior to introduction into the field.

Screening the Peanut Core Collection for Resistance to Tomato Spotted Wilt Virus

1996 ◽  
Vol 23 (1) ◽  
pp. 57-61 ◽  
Author(s):  
W. F. Anderson ◽  
C. C. Holbrook ◽  
A. K. Culbreath
Keyword(s):  
Core Collection ◽  
Tomato Spotted Wilt Virus ◽  
Disease Incidence ◽  
Field Trials ◽  
Stepwise Multiple Regression ◽  
Sources Of Resistance ◽  
Plant Introductions ◽  
Resistant Cultivars ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Abstract Tomato spotted wilt virus (TSWV) is among the greatest yield-reducing viruses affecting peanut (Arachis hypogaea L.). The best known method of control of TSWV is through the use of resistant cultivars. Unfortunately, only a few peanut genotypes with moderate levels of resistance are known. The objectives of this study were to identify additional sources of resistance to TSWV and to determine whether plant descriptor information is associated with reaction to TSWV in the field. Peanut plant introductions from a core collection were evaluated for resistance to TSWV in field trials from 1991 to 1993. Great variability was found among PIs for reaction to natural TSWV epidemics at Attapulgus, GA. Accessions which exhibited potential resistance in 1991 or 1992 were reevaluated in subsequent years. Disease pressure was high in 1993 and 27 accessions exhibited significantly greater resistance than Florunner, and one (PI 262049) had lower disease incidence than Southern Runner. Only minor relationships existed between TSWV incidence and plant descriptor traits (growth habit and maturity) using the stepwise multiple regression procedure. Peanut accessions with resistance to TSWV were found among all maturity levels and growth habits. These accessions provide additional parents and may provide additional genes for resistance that may be useful in developing resistant cultivars.

Effect of Imazapic and 2,4-DB on Peanut Yield, Grade, and Seed Germination

2010 ◽  
Vol 37 (1) ◽  
pp. 78-82 ◽  
Author(s):  
W. H. Faircloth ◽  
E. P. Prostko
Keyword(s):  
Seed Germination ◽  
Tomato Spotted Wilt Virus ◽  
Seed Quality ◽  
Field Trials ◽  
The Other ◽  
Peanut Seed ◽  
Stand Establishment ◽  
South Georgia ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Abstract Recurrent problems with peanut seed germination and stand establishment have raised concerns about the potential effects of herbicides on peanut seed quality. Field trials were conducted in south Georgia in 2005 and 2006 to evaluate the effects of imazapic and 2,4-DB on peanut yield, grade, incidence of tomato spotted wilt virus (TSWV), and seed germination. Imazapic at 71 g ai ha−1, applied at 30 and 40 days after planting (DAP), and 2,4-DB at 270 g ai ha−1, applied at 75 or 90 DAP, had no effect on peanut yield, grade, incidence of TSWV, and seed germination when averaged over two locations (Dawson and Tifton) and three cultivars (Georgia Green, Georgia-01R, and C-99R). Normal cultivar yield variations were observed; however, Georgia-01R had reduced standard (25 C) and cold (15 C) germination when compared to the other cultivars.

Variation Among Virginia Market-Type Peanut Genotypes in Susceptibility to Tomato Spotted Wilt Virus Vectored by Thrips (Thysanoptera: Thripidae)

2008 ◽  
Vol 35 (2) ◽  
pp. 92-100 ◽  
Author(s):  
S. D. Riniker ◽  
R. L. Brandenburg ◽  
G. G. Kennedy ◽  
T. G. Isleib ◽  
D. L. Jordan
Keyword(s):  
North Carolina ◽  
Tomato Spotted Wilt Virus ◽  
Field Tests ◽  
Breeding Line ◽  
Breeding Lines ◽  
Tomato Spotted Wilt ◽  
Foliar Symptoms ◽  
Arachis Hypogaea L ◽  
Highly Correlated ◽  
Wilt Virus

Abstract Tomato spotted wilt virus (TSWV), a thrips-vectored tospovirus, is an important pathogen of peanut (Arachis hypogaea L.). Development of tolerant cultivars has proven to be one of the most promising methods to manage the disease. Twenty-four genotypes of virginia market-type peanut were monitored in field tests for thrips damage, and TSWV incidence and severity during 2004 and 2005 in North Carolina. The cultivar Gregory had a higher density of adult thrips in foliage than any other genotype, while breeding lines N01057 and N03054E had the lowest density. No significant correlation was detected between thrips density or injury and TSWV incidence. Line N03036EJ had the greatest TSWV incidence, but did not differ from cultivars Gregory or Perry in incidence. Line N00033 had the least TSWV incidence and differed from the cultivars Gregory and Perry. The occurrence of late-season chlorosis or peanut yellowing death (PYD) was highly correlated with TSWV infection (P < 0.0001). Breeding line N02051ol had the greatest incidence of PYD, but did not differ statistically from Gregory or Perry. Lines N03023EF and N01083 had the least PYD incidence. Plants infected with TSWV not expressing foliar symptoms were found in far greater abundance than plants that were infected and symptomatic. Line N03036EJ had the greatest proportion of infected but asymptomatic plants; line N03054E had the least. Susceptible lines are more likely to become infected, rather than just more likely to show spotted wilt symptoms.

scholarly journals Tomato spotted wilt virus in peanut tissue types and physiological effects related to disease incidence and severity*

2005 ◽  
Vol 54 (4) ◽  
pp. 431-440 ◽  
Author(s):  
D. Rowland ◽  
J. Dorner ◽  
R. Sorensen ◽  
J. P. Beasley ◽  
J. Todd
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Disease Incidence ◽  
Physiological Effects ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

scholarly journals Evaluation of Arachis Species for Resistance to Tomato Spotted Wilt Virus

2002 ◽  
Vol 29 (2) ◽  
pp. 79-84 ◽  
Author(s):  
J. H. Lyerly ◽  
H. T. Stalker ◽  
J. W. Moyer ◽  
K. Hoffman
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Insect Pests ◽  
Disease Incidence ◽  
Diploid Species ◽  
Breeding Programs ◽  
Arachis Species ◽  
Tomato Spotted Wilt ◽  
Arachis Hypogaea L ◽  
Cultivated Species ◽  
Wilt Virus

Abstract Tomato spotted wilt virus (TSWV) is an important plant pathogen with a wide host range, including the domesticated peanut (Arachis hypogaea L.). After initial outbreaks on peanut during the 1980s, the virus has spread to all peanut-producing states in the U.S. TSWV is transmitted by several species of thrips which are difficult to control with insecticides; therefore, control of TSWV most likely will come from selecting resistant genotypes in breeding programs. Although moderate levels of resistance have been discovered in A. hypogaea, complete virus resistance has not been found. Several Arachis species have desirable genes for plant resistances and tolerate many disease and insect pests better than the cultivated species. The objectives of this study were to (a) evaluate TSWV disease incidence and severity in accessions of Arachis species, and (b) compare levels of TSWV resistance in diploid species to selected A. hypogaea genotypes. In this study, 46 diploid Arachis spp. accessions were evaluated in the greenhouse by artificial inoculation tests for resistance to TSWV. Nine Arachis accessions were observed with no disease symptoms when TSWV isolate 10 was used as opposed to A. hypogaea lines that ranged from moderately to highly susceptible. Additional testing with more virulent isolates identified A. diogoi accession GKP 10602 and A. correntina accession GKP 9530 as highly resistant to the virus. These two accessions are being used as parents in crossing programs to incorporate TSWV resistance genes into A. hypogaea.

scholarly journals First Report of Tomato spotted wilt virus on Chrysanthemum in Serbia

Plant Disease ◽  
2013 ◽  
Vol 97 (1) ◽  
pp. 150-150 ◽  
Author(s):  
I. Stanković ◽  
A. Bulajić ◽  
A. Vučurović ◽  
D. Ristić ◽  
K. Milojević ◽  
...  
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Disease Incidence ◽  
N Gene ◽  
Impatiens Necrotic Spot Virus ◽  
Maximum Parsimony Tree ◽  
Rt Pcr ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
Negative Controls ◽  
Wilt Virus

In July 2011, greenhouse-grown chrysanthemum hybrid plants (Chrysanthemum × morifolium) with symptoms resembling those associated with tospoviruses were observed in the Kupusina locality (West Bačka District, Serbia). Disease incidence was estimated at 40%. Symptomatic plants with chlorotic ring spots and line patterns were sampled and tested by double antibody sandwich (DAS)-ELISA using polyclonal antisera (Bioreba AG, Reinach, Switzerland) against the two of the most devastating tospoviruses in the greenhouse floriculture industry: Tomato spotted wilt virus (TSWV) and Impatiens necrotic spot virus (INSV) (2). Commercial positive and negative controls and extracts from healthy chrysanthemum tissue were included in each ELISA. TSWV was detected serologically in 16 of 20 chrysanthemum samples and all tested samples were negative for INSV. The virus was mechanically transmitted from ELISA-positive chrysanthemum samples to five plants each of both Petunia × hybrida and Nicotiana tabacum ‘Samsun’ using chilled 0.01 M phosphate buffer (pH 7) containing 0.1% sodium sulfite. Inoculated plants produced local necrotic spots and systemic chlorotic/necrotic concentric rings, consistent with symptoms caused by TSWV (1). The presence of TSWV in ELISA-positive chrysanthemum plants and N. tabacum‘Samsun’ was further confirmed by conventional reverse transcription (RT)-PCR. Total RNAs were extracted with an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). RT-PCR was performed with the One-Step RT-PCR Kit (Qiagen) using primers TSWVCP-f/TSWVCP-r specific to the nucleocapsid protein (N) gene (4). A Serbian isolate of TSWV from tobacco (GenBank Accession No. GQ373173) and RNA extracted from a healthy chrysanthemum plant were used as positive and negative controls, respectively. An amplicon of the correct predicted size (738-bp) was obtained from each of the plants assayed, and that derived from chrysanthemum isolate 529-11 was purified (QIAqick PCR Purification Kit, Qiagen) and sequenced (JQ692106). Sequence analysis of the partial N gene, conducted with MEGA5 software, revealed the highest nucleotide identity of 99.6% (99% amino acid identity) with 12 TSWV isolates deposited in GenBank originating from different hosts from Italy (HQ830186-87, DQ431237-38, DQ398945), Montenegro (GU355939-40, GU339506, GU339508), France (FR693055-56), and the Czech Republic (AJ296599). The consensus maximum parsimony tree obtained on a 705-bp partial N gene sequence of TSWV isolates available in GenBank revealed that Serbian TSWV isolate 529-11 from chrysanthemum was clustered in the European subpopulation 2, while the Serbian isolates from tomato (GU369723) and tobacco (GQ373172-73 and GQ355467) were clustered in the European subpopulation 1 denoted previously (3). The distribution of TSWV in commercial chrysanthemum crops is wide (2). To our knowledge, this is the first report of TSWV infecting chrysanthemum in Serbia. Since chrysanthemum popularity and returns have been rising rapidly, the presence of TSWV may significantly reduce quality of crops in Serbia. References: (1) Anonymous. OEPP/EPPO Bull. 34:271, 2004. (2) Daughtrey et al. Plant Dis. 81:1220, 1997. (3) I. Stanković et al. Acta Virol. 55:337, 2011. (4) A. Vučurović et al. Eur. J. Plant Pathol. 133:935, 2012.

scholarly journals First Report of Botrytis Blight of Peanut Caused by Botrytis cinerea in Georgia

Plant Disease ◽  
2005 ◽  
Vol 89 (8) ◽  
pp. 910-910 ◽  
Author(s):  
J. E. Woodward ◽  
T. B. Brenneman ◽  
R. C. Kemerait ◽  
A. K. Culbreath ◽  
J. R. Clark
Keyword(s):  
Botrytis Cinerea ◽  
Disease Incidence ◽  
Block Design ◽  
Gray Mold ◽  
American Phytopathological Society ◽  
Breeding Programs ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
Arachis Hypogaea L ◽  
Wilt Virus

Because of the importance of spotted wilt caused by Tomato spotted wilt virus (TSWV), most peanut (Arachis hypogaea L.) breeding programs in the southeastern United States are focusing on developing resistance to TSWV. Many of the cultivars with improved resistance to TSWV are late maturing, requiring 150 days to reach optimum maturity. This factor could greatly impact disease problems at harvest. During November of 2004, an unknown disease was observed on peanut cvs. Georgia 02-C and Hull in a commercial field in Appling County. Symptoms included wilting stems with water-soaked lesions and a dense, gray mold growing on infected tissues. Final disease incidence was less than 5%. For isolation, diseased tissue was surface sterilized by soaking in 0.5% sodium hypochlorite for 1 min, air dried, plated on potato dextrose agar (PDA), and incubated at 20°C. Botrytis cinerea Pers.:Fr., causal agent of Botrytis blight, was isolated from the margins of infected tissue. Mycelia were initially white but became gray after 72 h at which time tall, branched, septate conidiophores formed. Mature, unicellular, ellipsoid, hyaline conidia (8.9 × 10.4 μm) formed in botryose heads (1). Hard, black, irregular-shaped sclerotia formed after 2 weeks. Stems of greenhouse-grown peanut plants (cv. Georgia Green) were inoculated with PDA plugs colonized with either B. cinerea or B. allii Munn. Inoculations were made 3 cm below the last fully expanded leaf on wounded and nonwounded tissue. Noncolonized PDA plugs served as controls (n = 9). Plants were arranged in a dew chamber at 20°C in a randomized complete block design. Lesions and spore masses identical to those observed in the field appeared 3 to 5 days after being inoculated with B. cinerea. The B. allii inoculations caused only superficial lesions. After 5 days, mean lesion lengths for B. cinerea were 59 and 37 mm for wounded and nonwounded inoculations, respectively. B. cinerea was recovered from 100% of the symptomatic tissues. Botrytis blight is considered a late-season disease that occurs in cool, wet weather (3). Symptoms similar to those of Botrytis blight were observed on mature and over-mature peanut in Georgia and have been cited as “unpublished observations” (2); however, to our knowledge, this is the first report of the disease in Georgia. Although Botrytis blight is not considered a major peanut disease, it may become more prevalent at harvest as producers utilize late-maturing cultivars to manage spotted wilt. References: (1) H. L. Barnett and B. B. Hunter. Illustrated Guide of Imperfect Fungi. 4th ed. The American Phytopathological Society, St. Paul, MN, 1998. (2) K. H. Garren and C. Wilson. Peanut Diseases. Pages 262–333 in: The Peanut, the Unpredictable Legume. The National Fertilizer Assoc. Washington D.C. 1951. (3) D. M. Porter. Botrytis blight. Pages 10–11 in: Compendium of Peanut Diseases. 2nd ed. N. Kokalis-Burelle et al., eds. The American Phytopathological Society, St. Paul, MN. 1997.

Peanut plants in Queensland infected with tomato spotted wilt virus

1961 ◽  
Vol 12 (2) ◽  
pp. 239 ◽  
Author(s):  
K Helms ◽  
NE Grylls ◽  
GS Purss
Keyword(s):  
Arachis Hypogaea ◽  
Tomato Spotted Wilt Virus ◽  
Disease Incidence ◽  
Infective Virus ◽  
Tomato Spotted Wilt ◽  
Arachis Hypogaea L ◽  
Tagetes Minuta ◽  
New Hosts ◽  
The Mean ◽  
Wilt Virus

A disease of peanut (Arachis hypogaea L.) in Queensland, previously referred to as "chlorosis", was identified as being caused by the tomato spotted wilt virus. Symptoms of the disease on peanut are described. Erigeron bonariensis L., Tagetes minuta L., and Trifolium subterraneurn L. are recorded as new hosts. The disease was not seed-transmitted, although seeds developed lesions and were malformed. Infective virus was found in tissues of the integuments of immature seed. The maximum disease incidence recorded in an individual crop was 5.5%. The mean seed yield of diseased plants was 12.7 g per plant, whereas that of healthy plants was 128.2 g.

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