scholarly journals Evaluation of Arachis Species for Resistance to Tomato Spotted Wilt Virus

2002 ◽  
Vol 29 (2) ◽  
pp. 79-84 ◽  
Author(s):  
J. H. Lyerly ◽  
H. T. Stalker ◽  
J. W. Moyer ◽  
K. Hoffman
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Insect Pests ◽  
Disease Incidence ◽  
Diploid Species ◽  
Breeding Programs ◽  
Arachis Species ◽  
Tomato Spotted Wilt ◽  
Arachis Hypogaea L ◽  
Cultivated Species ◽  
Wilt Virus

Abstract Tomato spotted wilt virus (TSWV) is an important plant pathogen with a wide host range, including the domesticated peanut (Arachis hypogaea L.). After initial outbreaks on peanut during the 1980s, the virus has spread to all peanut-producing states in the U.S. TSWV is transmitted by several species of thrips which are difficult to control with insecticides; therefore, control of TSWV most likely will come from selecting resistant genotypes in breeding programs. Although moderate levels of resistance have been discovered in A. hypogaea, complete virus resistance has not been found. Several Arachis species have desirable genes for plant resistances and tolerate many disease and insect pests better than the cultivated species. The objectives of this study were to (a) evaluate TSWV disease incidence and severity in accessions of Arachis species, and (b) compare levels of TSWV resistance in diploid species to selected A. hypogaea genotypes. In this study, 46 diploid Arachis spp. accessions were evaluated in the greenhouse by artificial inoculation tests for resistance to TSWV. Nine Arachis accessions were observed with no disease symptoms when TSWV isolate 10 was used as opposed to A. hypogaea lines that ranged from moderately to highly susceptible. Additional testing with more virulent isolates identified A. diogoi accession GKP 10602 and A. correntina accession GKP 9530 as highly resistant to the virus. These two accessions are being used as parents in crossing programs to incorporate TSWV resistance genes into A. hypogaea.

scholarly journals First Report of Botrytis Blight of Peanut Caused by Botrytis cinerea in Georgia

Plant Disease ◽  
2005 ◽  
Vol 89 (8) ◽  
pp. 910-910 ◽  
Author(s):  
J. E. Woodward ◽  
T. B. Brenneman ◽  
R. C. Kemerait ◽  
A. K. Culbreath ◽  
J. R. Clark
Keyword(s):  
Botrytis Cinerea ◽  
Disease Incidence ◽  
Block Design ◽  
Gray Mold ◽  
American Phytopathological Society ◽  
Breeding Programs ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
Arachis Hypogaea L ◽  
Wilt Virus

Because of the importance of spotted wilt caused by Tomato spotted wilt virus (TSWV), most peanut (Arachis hypogaea L.) breeding programs in the southeastern United States are focusing on developing resistance to TSWV. Many of the cultivars with improved resistance to TSWV are late maturing, requiring 150 days to reach optimum maturity. This factor could greatly impact disease problems at harvest. During November of 2004, an unknown disease was observed on peanut cvs. Georgia 02-C and Hull in a commercial field in Appling County. Symptoms included wilting stems with water-soaked lesions and a dense, gray mold growing on infected tissues. Final disease incidence was less than 5%. For isolation, diseased tissue was surface sterilized by soaking in 0.5% sodium hypochlorite for 1 min, air dried, plated on potato dextrose agar (PDA), and incubated at 20°C. Botrytis cinerea Pers.:Fr., causal agent of Botrytis blight, was isolated from the margins of infected tissue. Mycelia were initially white but became gray after 72 h at which time tall, branched, septate conidiophores formed. Mature, unicellular, ellipsoid, hyaline conidia (8.9 × 10.4 μm) formed in botryose heads (1). Hard, black, irregular-shaped sclerotia formed after 2 weeks. Stems of greenhouse-grown peanut plants (cv. Georgia Green) were inoculated with PDA plugs colonized with either B. cinerea or B. allii Munn. Inoculations were made 3 cm below the last fully expanded leaf on wounded and nonwounded tissue. Noncolonized PDA plugs served as controls (n = 9). Plants were arranged in a dew chamber at 20°C in a randomized complete block design. Lesions and spore masses identical to those observed in the field appeared 3 to 5 days after being inoculated with B. cinerea. The B. allii inoculations caused only superficial lesions. After 5 days, mean lesion lengths for B. cinerea were 59 and 37 mm for wounded and nonwounded inoculations, respectively. B. cinerea was recovered from 100% of the symptomatic tissues. Botrytis blight is considered a late-season disease that occurs in cool, wet weather (3). Symptoms similar to those of Botrytis blight were observed on mature and over-mature peanut in Georgia and have been cited as “unpublished observations” (2); however, to our knowledge, this is the first report of the disease in Georgia. Although Botrytis blight is not considered a major peanut disease, it may become more prevalent at harvest as producers utilize late-maturing cultivars to manage spotted wilt. References: (1) H. L. Barnett and B. B. Hunter. Illustrated Guide of Imperfect Fungi. 4th ed. The American Phytopathological Society, St. Paul, MN, 1998. (2) K. H. Garren and C. Wilson. Peanut Diseases. Pages 262–333 in: The Peanut, the Unpredictable Legume. The National Fertilizer Assoc. Washington D.C. 1951. (3) D. M. Porter. Botrytis blight. Pages 10–11 in: Compendium of Peanut Diseases. 2nd ed. N. Kokalis-Burelle et al., eds. The American Phytopathological Society, St. Paul, MN. 1997.

Peanut plants in Queensland infected with tomato spotted wilt virus

1961 ◽  
Vol 12 (2) ◽  
pp. 239 ◽  
Author(s):  
K Helms ◽  
NE Grylls ◽  
GS Purss
Keyword(s):  
Arachis Hypogaea ◽  
Tomato Spotted Wilt Virus ◽  
Disease Incidence ◽  
Infective Virus ◽  
Tomato Spotted Wilt ◽  
Arachis Hypogaea L ◽  
Tagetes Minuta ◽  
New Hosts ◽  
The Mean ◽  
Wilt Virus

A disease of peanut (Arachis hypogaea L.) in Queensland, previously referred to as "chlorosis", was identified as being caused by the tomato spotted wilt virus. Symptoms of the disease on peanut are described. Erigeron bonariensis L., Tagetes minuta L., and Trifolium subterraneurn L. are recorded as new hosts. The disease was not seed-transmitted, although seeds developed lesions and were malformed. Infective virus was found in tissues of the integuments of immature seed. The maximum disease incidence recorded in an individual crop was 5.5%. The mean seed yield of diseased plants was 12.7 g per plant, whereas that of healthy plants was 128.2 g.

scholarly journals The First Report of Tomato spotted wilt virus on Gerbera and Chrysanthemun in Venezuela

Plant Disease ◽  
2014 ◽  
Vol 98 (8) ◽  
pp. 1161-1161 ◽  
Author(s):  
E. Marys ◽  
A. Mejías ◽  
E. Rodríguez-Román ◽  
D. Avilán ◽  
T. Hurtado ◽  
...  
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Disease Incidence ◽  
The United States ◽  
Cut Flowers ◽  
Rt Pcr ◽  
Tomato Spotted Wilt ◽  
Arachis Hypogaea L ◽  
Pcr Products ◽  
Ornamental Crops ◽  
Wilt Virus

Gerbera (Gerbera jamesonii) and Chrysanthemum (family Asteraceae) are two of the top 10 cut flowers of the world, with great commercial value. Since 1998, Venezuela began a floral industry to produce and export fresh cut gerbera and chrysanthemum, with 40% of nurseries concentrated in Altos Mirandinos (Miranda State, north central region of the country). For the past 2 years, greenhouse-grown gerbera and chrysanthemum have been observed displaying symptoms resembling those associated with tospoviruses. Symptomatic plants showed concentric rings, irregular chlorotic blotches, and deformation on leaves. Disease incidence was estimated at 30%. Mechanical inoculation with extracts of symptomatic leaves reproduced the typical concentric ring symptoms on indicator plants Arachis hypogaea L. cv. San Martín, Capsicum chinense, and G. jamesonii 6 to 15 days after inoculation. In initial tests, leaves from each 30 symptomatic gerbera and chrysanthemum species from several greenhouse facilities in Altos Mirandinos reacted positively when tested by DAS-ELISA with polyclonal antisera (ATCC, Rockville, MD) raised against Tomato spotted wilt virus (TSWV). Total RNA was extracted with the RNeasy Plant Mini kit (QIAGEN, Hilden, Germany) from two gerbera and two chrysanthemum ELISA-positive samples. The TSWV coat protein gene was amplified by conventional reverse transcription (RT)-PCR using primers CP1 TSWV (TTAACTTACAGCTGCTTT) and CP2 TSWV (CAAAGCATATAAGAACTT) (1). A single DNA product of ~823 bp was amplified from all samples. RT-PCR products were directly sequenced in both orientations and sequences were deposited in GenBank (Accession Nos. KF146700 and KF146701 derived from chrysanthemum, KF146702 and KF146703 derived from gerbera). The resulting sequences showed over 99% identity with each other. and were found to be closely related (over 99%) with TSWV isolates deposited in GenBank originating from different hosts from France (FR693058, FR693055), Montenegro (GU339506, GU339508, GU355940), Italy (HQ830187), New Zealand (KC494501), South Korea (KC261967), and the United States (AY744476). To our knowledge, this is the first confirmed report of TSWV infecting gerbera and chrysanthemum in Venezuela. The relatively widespread occurrence of TSWV in Miranda State underscores the need for systematic surveys to assess its incidence and impact on ornamental crops so that appropriate management tactics can be developed. Reference: (1) R. A. Mumford et al. J. Virol. Methods 57:109, 1996.

Genotype × Seeding Rate Interaction among TSWV-Resistant, Runner-Type Peanut Cultivars1

2003 ◽  
Vol 30 (2) ◽  
pp. 108-111 ◽  
Author(s):  
W. D. Branch ◽  
J. A. Baldwin ◽  
A. K. Culbreath
Keyword(s):  
Coastal Plain ◽  
Tomato Spotted Wilt Virus ◽  
Disease Incidence ◽  
Seeding Rate ◽  
Green It ◽  
Tomato Spotted Wilt ◽  
Arachis Hypogaea L ◽  
Peanut Production ◽  
Rate Interaction ◽  
Wilt Virus

Abstract Tomato spotted wilt virus (TSWV) resistant, runner-type peanut (Arachis hypogaea L.) cultivars are the most important defense to control spotted wilt disease in southeast U.S. peanut production. The objective of this 3-yr (1999–01) study was to evaluate six TSWV-resistant, runner-type cultivars (Southern Runner, Florida MDR 98, C-99R, ViruGard, Georgia Green, and Georgia-OIR) at three different seeding rates (3, 5, and 7 seed/30.5 cm) in single conventional row patterns for possible genotype (GE) × seeding rate (SR) interaction at the Univ. of Georgia, Coastal Plain Experiment Station. The combined split-plot analyses of variance resulted in highly significant (P ≤ 0.01) GE × SR interaction, which indicates that not all six runner-type cultivars performed the same at each of these three seeding rates. A good example was the TSWV-resistant, runner-type peanut cultivar Georgia Green. It performed subpar at the below normal or lowest seeding rate; whereas at the highest seeding rate, Georgia Green and Georgia-OIR produced the highest pod yields and dollar value returns per hectare among all of these runner-type cultivars. TSWV disease incidence was also significantly lower for the TSWV-resistant Georgia Green cultivar at each of the two higher seeding rates compared to the lowest seeding rate.

Variation Among Virginia Market-Type Peanut Genotypes in Susceptibility to Tomato Spotted Wilt Virus Vectored by Thrips (Thysanoptera: Thripidae)

2008 ◽  
Vol 35 (2) ◽  
pp. 92-100 ◽  
Author(s):  
S. D. Riniker ◽  
R. L. Brandenburg ◽  
G. G. Kennedy ◽  
T. G. Isleib ◽  
D. L. Jordan
Keyword(s):  
North Carolina ◽  
Tomato Spotted Wilt Virus ◽  
Field Tests ◽  
Breeding Line ◽  
Breeding Lines ◽  
Tomato Spotted Wilt ◽  
Foliar Symptoms ◽  
Arachis Hypogaea L ◽  
Highly Correlated ◽  
Wilt Virus

Abstract Tomato spotted wilt virus (TSWV), a thrips-vectored tospovirus, is an important pathogen of peanut (Arachis hypogaea L.). Development of tolerant cultivars has proven to be one of the most promising methods to manage the disease. Twenty-four genotypes of virginia market-type peanut were monitored in field tests for thrips damage, and TSWV incidence and severity during 2004 and 2005 in North Carolina. The cultivar Gregory had a higher density of adult thrips in foliage than any other genotype, while breeding lines N01057 and N03054E had the lowest density. No significant correlation was detected between thrips density or injury and TSWV incidence. Line N03036EJ had the greatest TSWV incidence, but did not differ from cultivars Gregory or Perry in incidence. Line N00033 had the least TSWV incidence and differed from the cultivars Gregory and Perry. The occurrence of late-season chlorosis or peanut yellowing death (PYD) was highly correlated with TSWV infection (P < 0.0001). Breeding line N02051ol had the greatest incidence of PYD, but did not differ statistically from Gregory or Perry. Lines N03023EF and N01083 had the least PYD incidence. Plants infected with TSWV not expressing foliar symptoms were found in far greater abundance than plants that were infected and symptomatic. Line N03036EJ had the greatest proportion of infected but asymptomatic plants; line N03054E had the least. Susceptible lines are more likely to become infected, rather than just more likely to show spotted wilt symptoms.

scholarly journals First Report of Tomato spotted wilt virus on Chrysanthemum in Serbia

Plant Disease ◽  
2013 ◽  
Vol 97 (1) ◽  
pp. 150-150 ◽  
Author(s):  
I. Stanković ◽  
A. Bulajić ◽  
A. Vučurović ◽  
D. Ristić ◽  
K. Milojević ◽  
...  
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Disease Incidence ◽  
N Gene ◽  
Impatiens Necrotic Spot Virus ◽  
Maximum Parsimony Tree ◽  
Rt Pcr ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
Negative Controls ◽  
Wilt Virus

In July 2011, greenhouse-grown chrysanthemum hybrid plants (Chrysanthemum × morifolium) with symptoms resembling those associated with tospoviruses were observed in the Kupusina locality (West Bačka District, Serbia). Disease incidence was estimated at 40%. Symptomatic plants with chlorotic ring spots and line patterns were sampled and tested by double antibody sandwich (DAS)-ELISA using polyclonal antisera (Bioreba AG, Reinach, Switzerland) against the two of the most devastating tospoviruses in the greenhouse floriculture industry: Tomato spotted wilt virus (TSWV) and Impatiens necrotic spot virus (INSV) (2). Commercial positive and negative controls and extracts from healthy chrysanthemum tissue were included in each ELISA. TSWV was detected serologically in 16 of 20 chrysanthemum samples and all tested samples were negative for INSV. The virus was mechanically transmitted from ELISA-positive chrysanthemum samples to five plants each of both Petunia × hybrida and Nicotiana tabacum ‘Samsun’ using chilled 0.01 M phosphate buffer (pH 7) containing 0.1% sodium sulfite. Inoculated plants produced local necrotic spots and systemic chlorotic/necrotic concentric rings, consistent with symptoms caused by TSWV (1). The presence of TSWV in ELISA-positive chrysanthemum plants and N. tabacum‘Samsun’ was further confirmed by conventional reverse transcription (RT)-PCR. Total RNAs were extracted with an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). RT-PCR was performed with the One-Step RT-PCR Kit (Qiagen) using primers TSWVCP-f/TSWVCP-r specific to the nucleocapsid protein (N) gene (4). A Serbian isolate of TSWV from tobacco (GenBank Accession No. GQ373173) and RNA extracted from a healthy chrysanthemum plant were used as positive and negative controls, respectively. An amplicon of the correct predicted size (738-bp) was obtained from each of the plants assayed, and that derived from chrysanthemum isolate 529-11 was purified (QIAqick PCR Purification Kit, Qiagen) and sequenced (JQ692106). Sequence analysis of the partial N gene, conducted with MEGA5 software, revealed the highest nucleotide identity of 99.6% (99% amino acid identity) with 12 TSWV isolates deposited in GenBank originating from different hosts from Italy (HQ830186-87, DQ431237-38, DQ398945), Montenegro (GU355939-40, GU339506, GU339508), France (FR693055-56), and the Czech Republic (AJ296599). The consensus maximum parsimony tree obtained on a 705-bp partial N gene sequence of TSWV isolates available in GenBank revealed that Serbian TSWV isolate 529-11 from chrysanthemum was clustered in the European subpopulation 2, while the Serbian isolates from tomato (GU369723) and tobacco (GQ373172-73 and GQ355467) were clustered in the European subpopulation 1 denoted previously (3). The distribution of TSWV in commercial chrysanthemum crops is wide (2). To our knowledge, this is the first report of TSWV infecting chrysanthemum in Serbia. Since chrysanthemum popularity and returns have been rising rapidly, the presence of TSWV may significantly reduce quality of crops in Serbia. References: (1) Anonymous. OEPP/EPPO Bull. 34:271, 2004. (2) Daughtrey et al. Plant Dis. 81:1220, 1997. (3) I. Stanković et al. Acta Virol. 55:337, 2011. (4) A. Vučurović et al. Eur. J. Plant Pathol. 133:935, 2012.

Response of Eight Peanut (Arachis hypogaea L.) Cultivars to the Herbicide AC 263,222

1995 ◽  
Vol 22 (1) ◽  
pp. 76-80 ◽  
Author(s):  
John S. Richburg ◽  
John W. Wilcut ◽  
Albert K. Culbreath ◽  
Craig K. Kvien
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Field Experiments ◽  
Stem Rot ◽  
Lower Percentage ◽  
Tomato Spotted Wilt ◽  
Arachis Hypogaea L ◽  
Main Effect ◽  
Peanut Cultivars ◽  
Ac 263,222 ◽  
Wilt Virus

Abstract Field experiments conducted in 1992 and 1993 in Georgia evaluated the response of the peanut cultivars Florunner, Georgia Runner, Goldkist GK 7, NC 7, NC-V11, Southern Runner, Sunrunner, and AgraTech VC-1 to AC 263,222 applied early-postemergence (EPOST) at 71 g ha-1. Visual injury, which was less than 13%, was independent of cultivar, and injury was not evident on any cultivar midseason. AC 263,222 slightly reduced canopy width consistently on all cultivars. The only significant effect on percentage fancy pods, medium, No. 1, sound splits, damaged, and other kernels was the main effect of cultivar. There was a significant cultivar, AC 263,222, and cultivar-by-AC 263,222 effect on extra large kernels (ELK) and jumbo kernel percentages. Southern Runner and NC-V11 had significantly lower percentage jumbo and ELK, respectively when treated with AC 263,222. In addition, Florunner and Sunrunner also had numerically less percentage jumbo kernels. Reductions in percentages of ELK and jumbo were probably the result of AC 263,222-induced maturity delays. However, these reductions were not reflected in peanut yields. The occurence of late and early leaf spot, tomato spotted wilt virus (TSWV), and southern stem rot was independent of both cultivar and AC 263,222.

scholarly journals Management of Tomato spotted wilt virus in Flue-Cured Tobacco with Acibenzolar-S-Methyl and Imidacloprid

Plant Disease ◽  
2001 ◽  
Vol 85 (3) ◽  
pp. 292-296 ◽  
Author(s):  
A. S. Csinos ◽  
H. R. Pappu ◽  
R. M. McPherson ◽  
M. G. Stephenson
Keyword(s):  
Field Trial ◽  
Tomato Spotted Wilt Virus ◽  
Disease Incidence ◽  
Field Trials ◽  
Growth And Yield ◽  
Yield Potential ◽  
Phytophthora Parasitica ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Effect On Growth

Tomato spotted wilt virus (TSWV) continues to be a major production constraint in flue-cured tobacco in Georgia. Pretransplant applications of acibenzolar-S-methyl (Actigard) and imidacloprid (Admire and Provado) were evaluated in field trials, singly and in combination, in four locations in 1999. Acibenzolar-S-methyl was also evaluated for its effect on growth and yield, potential phytotoxicity, and activity against tobacco blackshank incited by Phytophthora parasitica var. nicotianae. Acibenzolar-S-methyl alone and with imidacloprid significantly reduced TSWV incidence in all four locations, but the disease incidence in the imidacloprid-treated plots was lower in only one of the four sites. Applications of acibenzolar-S-methyl starting at 4 weeks posttransplant had no effect on TSWV incidence, size, or yield of tobacco compared with nontreated plots. Acibenzolar-S-methyl did not reduce blackshank in a field trial. In the greenhouse, rates of acibenzolar-S-methyl ranging from 0.25 to 8 g a.i. per 7,000 plants showed reductions in growth that did not seem to be related to rate. Pretransplant applications of acibenzolar-S-methyl were critical to the suppression of TSWV, while initial applications made posttransplant had no effect, suggesting that plants must be protected prior to introduction into the field.

scholarly journals Survey by ELISA of Thrips (Thysanoptera: Thripidae) Vectored Tomato Spotted Wilt Virus Distribution in Foliage and Flowers of Field-Infected Peanut1

1995 ◽  
Vol 22 (2) ◽  
pp. 141-149 ◽  
Author(s):  
Kenya K. Kresta ◽  
Forrest L. Mitchell ◽  
J. W. Smith
Keyword(s):  
Arachis Hypogaea ◽  
Immunosorbent Assay ◽  
Tomato Spotted Wilt Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Concentration ◽  
Tomato Spotted Wilt ◽  
Arachis Hypogaea L ◽  
Wilt Virus ◽  
Virus Distribution

Abstract The foliage of individual field-infected peanut plants (Arachis hypogaea L.) was examined by enzyme-linked immunosorbent assay (ELISA) for the presence of tomato spotted wilt virus (TSWV). TSWV was detected commonly in terminals (folded quadrifoliates) and flowers, the vector's feeding and breeding niches. Reconstructions of the assayed plants demonstrated that the virus concentration varied from plant to plant and symptoms were significantly correlated to virus concentration in individual leaves and terminals. The virus was not distributed uniformly throughout individual plants but was concentrated in young, developing terminal tissue. The presence of symptoms was indicative of detectable virus 95% of the time.

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