Characterization of Rhizoctonia spp. Causing Root and Stem Rot of Miniature Rose

Root and stem rot of miniature rose (Rosa hybrida L.) was observed in commercial glasshouse-grown roses in Gifu prefecture, Japan, during the summer and fall of 1997 and 1998. One hundred and fifty-three isolates of Rhizoctonia spp. were obtained from infected roots and stems. Of the 153 isolates, 9 had binucleate and 144 had multinucleate vegetative hyphal cells. Binucleate Rhizoctonia failed to anastomose with tester isolates of anastomosis groups (AG)-A through -S (not including AG-J and AG-M). Of 144 isolates identified as R. solani, 83.3% were AG 2-2 IIIB and 16.7% were AG 4 HG-I. Five isolates from each group caused severe rot and mortality on cuttings during rooting. Pathogenicity of Rhizoctonia spp. varied on three different ages of miniature roses cv. Silk. Isolates of AG 4 HG-I caused root and stem rot and mortality on 15-, 25-, and 40-day-old plants, whereas isolates of AG-2-2 IIIB caused root and stem rot and mortality on 15- and 25-day-old plants, but light root rot on 40-day-old plants. Isolates of binucleate Rhizoctonia caused root and stem rot and mortality only on 15-day-old plants.

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rDNA-based characterization of a new binucleate Rhizoctonia spp. causing root rot on kale in Brazil

European Journal of Plant Pathology ◽

10.1007/s10658-007-9175-z ◽

2007 ◽

Vol 119 (4) ◽

pp. 469-475 ◽

Cited By ~ 6

Author(s):

Eiko E. Kuramae ◽

Alexandre L. Buzeto ◽

Andreia K. Nakatani ◽

Nilton L. Souza

Keyword(s):

Root Rot ◽

Binucleate Rhizoctonia

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Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia

Pesticidi i fitomedicina ◽

10.2298/pif1802097v ◽

2018 ◽

Vol 33 (2) ◽

pp. 97-107 ◽

Cited By ~ 1

Author(s):

Mira Vojvodic ◽

Brankica Tanovic ◽

Milica Mihajlovic ◽

Petar Mitrovic ◽

Ivana Vico ◽

...

Keyword(s):

Root Rot ◽

Disease Incidence ◽

Morphological Characteristics ◽

Limiting Factors ◽

Black Root Rot ◽

Binucleate Rhizoctonia ◽

Cultivar Selection ◽

The Usa ◽

Agricultural Business

Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet.

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Characterization of Rhizoctonia-Like Fungi Isolated from Agronomic Crops and Turfgrasses in Mississippi

Plant Disease ◽

10.1094/pdis-91-3-0260 ◽

2007 ◽

Vol 91 (3) ◽

pp. 260-265 ◽

Cited By ~ 13

Author(s):

M. Tomaso-Peterson ◽

L. E. Trevathan

Keyword(s):

Diverse Group ◽

First Report ◽

Binucleate Rhizoctonia ◽

Anastomosis Groups ◽

Agronomic Crops ◽

Sharp Eyespot ◽

Rhizoctonia Zeae

Twenty-three isolates of Rhizoctonia spp. from agronomic crops and turfgrasses were characterized by cytological and pathological methods in order to establish the identity, pathogenicity, and virulence of Rhizoctonia spp. and anastomosis groups that occur on these hosts in Mississippi. Twelve isolates were identified as R. solani, including the five anastomosis groups (AGs) AG-1-IB, AG-2-2, AG-4, AG-5, and AG-13. Rhizoctonia zeae, R. oryzae, and eight binucleate Rhizoctonia sp., including R. cerealis, also were identified. R. solani AG-4 isolates were consistently the most virulent isolates on all hosts in pathogenicity evaluations. Pathogenicity of AG-2-2 and AG-5 isolates, binucleate Rhizoctonia spp., and R. oryzae varied between hosts. Two AG-2-2 isolates from bermudagrass or wheat were determined to be clonal isolates, with numerous self-anastomosis reactions. R. solani (AG-1-IB) was pathogenic on all graminaceous hosts. R. cerealis produced sharp eyespot symptoms on wheat and corn and minimal symptoms on cotton and soybean. This is a first report of R. cerealis as a pathogen of corn. R. zeae isolates were pathogenic on all hosts, including cotton and soybean. These results indicate that a diverse group of Rhizoctonia spp. occurs as pathogens on a wide variety of agronomic crops and turfgrasses in Mississippi.

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Characterization of isolates of binucleate Rhizoctonia spp. associated with strawberry black root rot complex using fatty acid methyl ester (FAME) profiles

Journal of General Plant Pathology ◽

10.1007/s10327-006-0286-8 ◽

2006 ◽

Vol 72 (5) ◽

pp. 318-322 ◽

Cited By ~ 2

Author(s):

Masaru Matsumoto ◽

Takemi Yoshida

Keyword(s):

Fatty Acid ◽

Methyl Ester ◽

Fatty Acid Methyl Ester ◽

Root Rot ◽

Acid Methyl Ester ◽

Black Root Rot ◽

Binucleate Rhizoctonia ◽

Acid Methyl

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Anastomosis Groups and Pathogenicity of Rhizoctonia solani and Binucleate Rhizoctonia from Potato in South Africa

Plant Disease ◽

10.1094/pdis-02-15-0236-re ◽

2015 ◽

Vol 99 (12) ◽

pp. 1790-1802 ◽

Cited By ~ 17

Author(s):

N. Muzhinji ◽

M. Truter ◽

J. W. Woodhall ◽

J. E. van der Waals

Keyword(s):

South Africa ◽

Rhizoctonia Solani ◽

Stem Canker ◽

Black Scurf ◽

Bootstrap Support ◽

Internal Transcribed Spacer Region ◽

Binucleate Rhizoctonia ◽

Anastomosis Groups ◽

Potato Plants ◽

Comprehensive Survey

A survey of anastomosis groups (AG) of Rhizoctonia spp. associated with potato diseases was conducted in South Africa. In total, 112 Rhizoctonia solani and 19 binucleate Rhizoctonia (BNR) isolates were recovered from diseased potato plants, characterized for AG and pathogenicity. The AG identity of the isolates was confirmed using phylogenetic analysis of the internal transcribed spacer region of ribosomal DNA. R. solani isolates recovered belonged to AG 3-PT, AG 2-2IIIB, AG 4HG-I, AG 4HG-III, and AG 5, while BNR isolates belonged to AG A and AG R, with frequencies of 74, 6.1, 2.3, 2.3, 0.8, 12.2, and 2.3%, respectively. R. solani AG 3-PT was the most predominant AG and occurred in all the potato-growing regions sampled, whereas the other AG occurred in distinct locations. Different AG grouped into distinct clades, with high maximum parsimony and maximum-likelihood bootstrap support for both R. solani and BNR. An experiment under greenhouse conditions with representative isolates from different AG showed differences in aggressiveness between and within AG. Isolates of AG 2-2IIIB, AG 4HG-III, and AG R were the most aggressive in causing stem canker while AG 3-PT, AG 5, and AG R caused black scurf. This is the first comprehensive survey of R. solani and BNR on potato in South Africa using a molecular-based approach. This is the first report of R. solani AG 2-2IIIB and AG 4 HG-I causing stem and stolon canker and BNR AG A and AG R causing stem canker and black scurf on potato in South Africa.

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Chemical characterization of glomerular and tubular basement membranes of cattle of different ages

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/0005-2736(78)90294-8 ◽

1978 ◽

Vol 514 (2) ◽

pp. 225-238 ◽

Cited By ~ 13

Author(s):

J.P.M. Langeveld ◽

J.H. Veerkamp ◽

L.A.H. Monnens ◽

U.J.G. van Haelst

Keyword(s):

Chemical Characterization ◽

Basement Membranes ◽

Different Ages

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Characterization of Fusarium oxysporum Isolates Obtained from Cucumber in China by Pathogenicity, VCG, and RAPD

Plant Disease ◽

10.1094/pdis.2004.88.6.645 ◽

2004 ◽

Vol 88 (6) ◽

pp. 645-649 ◽

Cited By ~ 45

Author(s):

D. J. Vakalounakis ◽

Z. Wang ◽

G. A. Fragkiadakis ◽

G. N. Skaracis ◽

D.-B. Li

Keyword(s):

Fusarium Oxysporum ◽

Random Amplified Polymorphic Dna ◽

Vegetative Compatibility ◽

Stem Rot ◽

Chinese Isolate ◽

Vegetative Compatibility Group ◽

Group I ◽

Compatibility Group ◽

First Time

Thirty-four isolates of Fusarium oxysporum, obtained in China from cucumber plants showing either Fusarium wilt (F. oxysporum f. sp. cucumerinum) or root and stem rot (F. oxysporum f. sp. radicis-cucumerinum) symptoms, were characterized by pathogenicity, vegetative compatibility, and random amplified polymorphic DNA (RAPD). Of these, 23 isolates were identified by pathogenicity as F. oxysporum f. sp. cucumerinum, and one as F. oxysporum f. sp. radicis-cucumerinum, while 10 isolates were avirulent on cucumber, melon, sponge gourd, and pumpkin. The Chinese isolates of F. oxysporum f. sp. cucumerinum were assigned to RAPD groups III and XXI and to vegetative compatibility group (VCG) 0183, four new VCGs, 0184 to 0187, and a single-member VCG included in the artificial VCG 018-. The Chinese isolate of F. oxysporum f. sp. radicis-cucumerinum was assigned to RAPD group I and bridging VCG 0260/0261. The occurrence of F. oxysporum f. sp. radicis-cucumerinum on cucumber is reported for the first time in China.

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