scholarly journals First Report of Beet soil-borne virus on Sugar Beet in Iran

Plant Disease ◽  
2002 ◽  
Vol 86 (2) ◽  
pp. 187-187 ◽  
Author(s):  
Sh. Farzadfar ◽  
R. Pourrahim ◽  
A. R. Golnaraghi ◽  
N. Shahraeen
Keyword(s):  
Sugar Beet ◽  
Chenopodium Quinoa ◽  
Polymyxa Betae ◽  
Main Field ◽  
First Report ◽  
Root Extracts ◽  
Beet Root ◽  
Tissue Blot Immunoassay ◽  
Sugar Beet Root ◽  
Fibrous Roots

Sugar beet is a main field crop in Iran and is cultivated in 186,000 ha. During the summer of 2001, sugar beet (Beta vulgaris) plants with pale, often upright, narrow, and rolled leaves were collected from the six main beet cultivation provinces of Iran (Fars, Ghazvin, Kermanshah, Khorasan, Semnan, and Isfahan). Roots of symptomatic plants were small, often with constriction, and exhibited warty outgrowth, proliferation of fibrous roots, and vascular necrosis. Beet soil-borne virus (BSBV) and Beet necrotic yellow vein (BNYVV, genus Benyvirus) were detected in sugar beet root samples by tissue-blot immunoassay (TBIA) using BSBV- and BNYVV-specific monoclonal antibodies (As-0576.1 and As-0799.1/CG6-F4, respectively; DSMZ Plant Virus Collection, Germany). Root extracts of sugar beet plants infected with BSBV, were infective by mechanical inoculation to Chenopodium quinoa causing necrotic ring spots. BSBV was detected in inoculated plants by TBIA. Laboratory tests using TBIA on 2,387 randomly collected samples showed that BSBV was present in 406 plants (17%) and BNYVV was present in 1,347 plants (56.43%). BSBV resembles BNYVV, the causal agent of sugar beet rhizomania, morphologically and in its transmission by Polymyxa betae (1). BNYVV has been reported previously from Iran (2). To our knowledge, this is the first report of BSBV occurring on sugar beet in Iran. References: (1) M. Ivanovic and I. Macfarlane. Annu. Rep. Rothamsted Exp. Stn. Page 190, 1982. (2) K. Izadpanah et al. Iran. J. Plant Pathol. 32:155, 1996.

scholarly journals First report of Pythium ultimum causing damping-off of Sugar beet (Beta vulgaris. L) in Montana, USA

Plant Disease ◽  
2020 ◽  
Author(s):  
Mohamed Fizal Khan ◽  
Md. Ehsanul Haque ◽  
Peter Hakk ◽  
Md. Ziaur Rahman Bhuyian ◽  
Yangxi Liu ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Pythium Ultimum ◽  
Pathogenicity Test ◽  
Damping Off ◽  
First Report ◽  
Beet Root ◽  
Pcr Products ◽  
Seed Rot ◽  
Sugar Beet Root

Sugar beet (Beta vulgaris L.) is a globally important crop for sugar. In May 2019, sugar beet seedlings were observed with wilting, lodging and a few were dead in Glendive (46.970170, -104.838204), Montana. Symptoms appeared near the soil line as the stem (hypocotyl) turned dark brown to black with characteristic thread-like infections which resembled Pythium damping-off. It affected approximately 10% of the growing seedlings. Diseased sugar beet root tissues were excised with a sterile scalpel and small pieces (10 mm²) were surface sterilized with 70 % ethanol for 30 seconds, rinsed twice with autoclaved water, air-dried and transferred to potato dextrose agar (PDA) media amended with pimaricin-vancomycin-PCNB (Conway, 1985). Four plates were incubated at 25° C in the dark (Masago et al., 1977) and two weeks later white, dense colony was observed (Zhang et al., 2018). The terminal smooth, globose oogonia (average 18.5 µm in diameter) and antheridia (average 14.5 × 9.5 µm) extended below the oogonium were observed via VWR N. A. 0.30 microscope. The morphological features of the four isolates were consistent with Pythium ultimum Trow (Watanabe, 2002). Genomic DNAs (NORGEN BIOTEK CORP, Fungi DNA Isolation Kit #26200) of four isolates were used for polymerase chain reaction (PCR) with the ITS6-ITS7 primers (Taheri et al., 2017). Subsequently, PCR products were flushed by E.Z.N.A ®Cycle Pure Kit, OMEGA and four samples were sent for Sanger sequencing to GenScript (GenScript, Piscataway, NJ). The sequences were identical and submitted to GenBank, NCBI (accession no. MN398593). The NCBI Blast analysis showed 100% sequence homology to Pythium ultimum with the following GenBank accessions; KF181451.1, KF181449.1 and AY598657.2. Pathogenicity test was done on sugar beet with the same isolates in the greenhouse. Two week old, pythium culture was mixed with vermiculite and perlite mixer (PRO-MIX FLX) in the plastic trays (24´´ x 15´´× 3˝), (22 °C, 75% Relaive Humidity). Sterile water (500 ml/each tray) was added in the mixer to provide sufficient moisture. Twenty seeds of cv. Hilleshog 4302 were sown in the tray, and the trays were replicated thrice with inoculated and mock treatments. Plants were watered as needed to maintain adequate soil moisture conducive for plant growth and disease development. Seven days after sowing, 50% and 100% germination was observed in the inoculated and control treatments, respectively. At the beginning of the second week, 30% post-emergence damping-off was observed in the inoculated treatments. Diseased seedlings were gently pulled out from the pots where similar symptoms were observed in the sugar beet seedlings as described previously. No incidence of disease was observed in mock-treated seedlings. Consistent reisolation of Pythium ultimum was morphologically and molecularly confirmed from the diseased seedlings, thus fulfilling Koch’s postulates. Pythium spp identification is prerequisite to develop effective management of pre and post-emergence damping-off. Pythium ultimum was previously reported in Nebraska to cause sugar beet seed rot and pre-emergence damping-off (Harvenson 2006). To our knowledge, this is the first report of Pythium ultimum causing damping-off on sugar beet in the Sidney factory district in Montana.

scholarly journals First Report of Beet virus Q on Sugar Beet in Iran

Plant Disease ◽  
2005 ◽  
Vol 89 (12) ◽  
pp. 1359-1359 ◽  
Author(s):  
Sh. Farzadfar ◽  
R. Pourrahim ◽  
A. R. Golnaraghi ◽  
A. Ahoonmanesh
Keyword(s):  
Sugar Beet ◽  
Type A ◽  
Single Strand Conformation Polymorphism ◽  
Natural Occurrence ◽  
Polymyxa Betae ◽  
Rt Pcr ◽  
First Report ◽  
Beet Root ◽  
Almost All ◽  
Beet Virus Q

During the 2001 growing season, a survey was conducted to determine the incidence of Beet necrotic yellow vein virus (BNYVV), Beet soilborne virus (BSBV), and Beet virus Q (BVQ) in Iran. A total of 2,816 random and 76 samples with rhizomania were collected from 131 fields in the main sugar beet cultivation areas of 13 provinces in Iran. All samples were tested using a tissue-blot immunoassay (TBIA) with commercial BNYVV (As-0799.1/CG6-F4), BSBV (As-0576.1), and BSBV/BVQ (As-0576.2) antisera provided by S. Winter (DSMZ, Braunschweig, Germany). For randomly collected samples, the highest incidence of virus infection was found for BNYVV (52.3%), followed by BSBV (9.5%) and BVQ (1.5%). Co-infection of BNYVV with BSBV or BVQ was 6.6% and 0.9%, respectively. Infection with both BSBV and BVQ was found in 16 (0.6%) samples. In addition, 0.4% (12) of the samples was infected with all three viruses. Our results indicated the presence of BVQ in samples from 10 fields located in Azarbayejan-e-gharbi, Esfahan, Fars, Kermanshah, Khorasan, Lorestan, and Semnan provinces of Iran, with or without rhizomania-like symptoms. The presence of viruses was confirmed using reverse transcription-polymerase chain reaction (RT-PCR) of RNA from 81, 19, and 14 root samples with positive reaction in TBIA to BNYVV, BSBV, and BVQ, respectively, with previously described primers (3,4). The primers specifically amplified fragments of 501 bp, 602 bp, 399 bp, and 291 bp of the BNYVV RNAs 1 and 4, BSBV RNA-2, and BVQ RNA-1, respectively. Our results indicated that the samples tested were also positive using RT-PCR. The putative vector for BNYVV, BSBV, and BVQ, Polymyxa betae, was also detected in 161 samples (from 127 fields) by amplification of a 170-bp fragment of the P. betae repetitive EcoRI-like fragments using previously described primers (4). RT-PCR products from 72 BNYVV-positive sugar beet root samples from 58 fields that also gave positive reactions in TBIA were analyzed using single-strand conformation polymorphism (SSCP) as previously described with extracts from root beards of the susceptible sugar beet cvs. OPUS and IC1 grown in the soils infested with BNYVV types A and B (provided by A. Meunier, Unite de Phytopathologie-UCL-AGRO-BAPA, Louvain-la-Neuve, Belgium) as positive controls (3). The patterns obtained with SSCP were uniform and showed widespread occurrence of BNYVV type A in almost all provinces surveyed. The fragments obtained for BNYVV RNAs 1 and 4 of an isolate from Qazvin (BNQ1) were sequenced (GenBank Accession Nos. AY703452 and AY703455) and compared with other sequences available in GenBank using Clustal W, which revealed 99.3 and 99.6% identity with the Japanese S (D84410) and Italian type A (AF197552) isolates, respectively. The economic importance of BVQ and its interactions with other sugar beet soilborne viruses remains a matter of debate. BNYVV and BSBV have been previously reported from Iran (1,2). To our knowledge, this is the first report of the natural occurrence of BVQ in sugar beets in Iran. References: (1) Sh. Farzadfar et al. Plant Dis. 86:187, 2002. (2) K. Izadpanah et al. Iran. J. Plant Pathol. 32:155, 1996. (3) R. Koenig et al. J. Gen. Virol. 76:2051, 1995. (4) A. Meunier et al. Appl. Environ. Microbiol. 69:2356, 2003.

Computing Model for Vibratory Digging-Out of Sugar Beet Roots

2014 ◽  
Vol 1 (2) ◽  
pp. 52-60
Author(s):  
V. Bulgakov ◽  
V. Adamchuk ◽  
H. Kaletnyk
Keyword(s):  
Differential Equations ◽  
Sugar Beet ◽  
Vertical Plane ◽  
Forward Motion ◽  
Admissible Velocity ◽  
Beet Root ◽  
Optimum Speed ◽  
Analytical Research ◽  
Sugar Beet Root ◽  
The Given

The new design mathematical model of the sugar beet roots vibration digging-out process with the plowshare vibration digging working part has been created. In this case the sugar beet root is simulated as a solid body , while the plowshare vibration digging working part accomplishes fl uctuations in the longitudinal - vertical plane with the given amplitude and frequency in the process of work . The aim of the current research has been to determine the dependences between the design and kinematic parameters of the sugar beet roots vibra- tion digging-out technological process from soil , which provide the ir non-damage. Methods . For the aim ac- complishment, the methods of design mathematical models constructing based on the classical laws of me- chanics are applied. The solution of the obtained differential equations is accomplished with the PC involve- ment. Results . The differential equations of the sugar beet root’s motion in course of the vibration digging-out have been comprised . They allow to determine the admissible velocity of the vibration digging working part’s forward motion depending on the angular parameters of the latter. In the result of the computational simula- tion i.e., the solution of the obtained analytical dependence by PC, the graphic dependences of the admissible velocity of plowshare v ibration digging working part’s forward motion providing the extraction of the sugar beet root from soil without the breaking-off of its tail section have been determined. Conclusions . Due to the performed analytical research , it has been established that γ = 13 ... 16 ° , β = 20 ... 30 ° should be considered as the most reasonable values of γ and β angles of the vibration digging working part providing both its forward motion optimum speed and sugar beet root digging-out from the soil without damage . On the ground of the data obtained from the analytical rese arch, the new vibration digging working parts for the sugar beet roots have been designed; also the patents of Ukraine for the inventions have been obtained for them.

Notes on Behaviour of Pemphigus betae Doane (Homoptera: Aphididae) Infected with Entomophthora aphidis Hoffm.

1958 ◽  
Vol 90 (7) ◽  
pp. 439-440 ◽  
Author(s):  
A. M. Harper
Keyword(s):  
Sugar Beet ◽  
Acyrthosiphon Pisum ◽  
Soil Surface ◽  
First Record ◽  
Department Of Agriculture ◽  
Beet Root ◽  
Pemphigus Betae ◽  
Below Ground ◽  
Sugar Beet Root ◽  
Annapolis Valley

Normally the sugar-beet root aphid, Pemphigus betae Doane, lives and feeds on sugar-beet roots below ground during the summer and fall. However, in many beet fields between Lethbridge and Monarch, Alberta, in September, 1956, a large number of these aphids were found on the soil surface and on the crowns and leaves of the plants. Many of the aphids had crawled up the plants and, even after death, remained clinging to the leaves (Fig. 1). This clinging reaction seemed similar to that of grasshoppers infected with Entomophthora grylli Fresen. Mr. R. B. Baird, Entomology Laboratory, Canada Department of Agriculture, Belleville, Ontario, identified the organism causing the disease destroying these aphids as Entomophthora aphidis Hoffm. This is the first record of this disease killing subterranean aphids in Canada. The only other reports of E. aphidis on root aphids are those of Maxson (1916) in Colorado and Charles (1941) in California. In Canada, it has been previously reported as a factor in control of the pea aphid, Acyrthosiphon pisum (Harr.), in the Annapolis Valley, Nova Scotia (MacLeod, 1953).

scholarly journals Control of Volunteer Glyphosate-Resistant Canola in Glyphosate-Resistant Sugar Beet

2015 ◽  
Vol 29 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Vipan Kumar ◽  
Prashant Jha
Keyword(s):  
Sugar Beet ◽  
Field Experiments ◽  
Agricultural Research ◽  
Effective Control ◽  
Additional Advantage ◽  
Leaf Stage ◽  
Beet Root ◽  
Control Volunteer ◽  
Sugar Beet Root ◽  
Herbicide Programs

Occurrence of glyphosate-resistant (GR) canola volunteers in GR sugar beet is a management concern for growers in the Northern Great Plains. Field experiments were conducted at the Southern Agricultural Research Center near Huntley, MT, in 2011 and 2012 to evaluate effective herbicide programs to control volunteer GR canola in GR sugar beet. Single POST application of triflusulfuron methyl alone at the two-leaf stage of sugar beet was more effective at 35 compared with 17.5 g ai ha−1. However, rate differences were not evident when triflusulfuron methyl was applied as a sequential POST (two-leaf followed by [fb] six-leaf stage of sugar beet) program (17.5 fb 17.5 or 35 fb 35 g ha−1). Volunteer GR canola plants in the sequential POST triflusulfuron methyl–containing treatments produced little biomass (11 to 15% of nontreated plots) but a significant amount of seeds (160 to 661 seeds m−2). Ethofumesate (4,200 g ai ha−1) PRE followed by sequential POST triflusulfuron methyl (17.5 or 35 g ha−1) provided effective control (94 to 98% at 30 d after treatment [DAT]), biomass reduction (97%), and seed prevention of volunteer GR canola. There was no additional advantage of adding either desmedipham + phenmedipham + ethofumesate premix (44.7 g ha−1) or ethofumesate (140 g ha−1) to the sequential POST triflusulfuron methyl–only treatments. The sequential POST ethofumesate-only (140 fb 140 g ha−1) treatment provided poor volunteer GR canola control at 30 DAT, and the noncontrolled plants produced 6,361 seeds m−2, which was comparable to the nontreated control (7,593 seeds m−2). Sequential POST triflusulfuron methyl–containing treatments reduced GR sugar beet root and sucrose yields to 18 and 20%, respectively. Consistent with GR canola control, sugar beet root and sucrose yields were highest (95 and 91% of hand-weeded plots, respectively) when the sequential POST triflusulfuron methyl–containing treatments were preceded by ethofumesate (4,200 g ha−1) PRE. Growers should utilize these effective herbicide programs to control volunteer GR canola in GR sugar beet. Because of high canola seed production potential, as evident from this research, control efforts should be aimed at preventing seed bank replenishment of the GR canola volunteers.

TECHNOLOGICAL QUALITIES OF SUGAR BEET ROOT CROPS AT DIFFERENT SOWING PERIODS

2021 ◽  
pp. 33-39
Author(s):  
Rafik I. Enikiev ◽  
Damir R. Islamgulov
Keyword(s):  
Sugar Beet ◽  
Beet Root ◽  
Root Crops ◽  
Sugar Beet Root

scholarly journals Influence of fertilization and nitrate-nitrogen position in soil profile on the sugar beet root yield and quality

2008 ◽  
Vol 53 (2) ◽  
pp. 83-90
Author(s):  
Goran Jacimovic ◽  
Branko Marinkovic ◽  
Jovan Crnobarac ◽  
Darinka Bogdanovic ◽  
Lazar Kovacev ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Nitrate Nitrogen ◽  
Quality Parameters ◽  
Sugar Yield ◽  
Root Yield ◽  
Negative Effects ◽  
Beet Root ◽  
Yield And Quality ◽  
Sugar Beet Root ◽  
Root Quality

Researches, which have lasted for two years, were carried out on long-term trial field at Rimski Sancevi, Novi Sad, Serbia. In this trial, the eight fertilization variants of N, P2O5 and K2O increased amounts were studied. Sugar beet root and tops yields were determined, as well as the elements of technological sugar beet root quality. Based on these results, percentage of sugar utilization and refined sugar yield was defined. In the spring, before applying of N fertilizer, amount of nitrate nitrogen in the soil and its influence on yield and quality was determined. The highest root yield in 2002 was produced at the variant N100 P150 K150, and in 2003 at the variant N150 P150 K150. However, in both years, referring to the variant N100 P100 K100, the differences were not statistically significant. Increasing of nitrogen amounts had negative effects on refined sugar yield. Amounts of NO3-N in the soil in spring, before sugar beet sowing, in 2002 had significant influence on root yield and refined sugar yield. In the year 2003, which was highly dry, high correlation ratio were gained between amounts of NO3-N in the soil and root quality parameters, but it wasn't significant between nitrogen amounts and root and refined sugar yield.

Sugar beet root as an organ for sucrose accumulation

1981 ◽  
pp. 209-213 ◽  
Author(s):  
Valentina P. Kholodova ◽  
Yuliya P. Bolyakina ◽  
Anatoli B. Meshcheriakov ◽  
Marina S. Orlova
Keyword(s):  
Sugar Beet ◽  
Sucrose Accumulation ◽  
Beet Root ◽  
Sugar Beet Root
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