scholarly journals Anastomosis Grouping of Rhizoctonia solani Associated with Black Scurf and Stem Canker of Potato in South Africa

Plant Disease ◽  
2004 ◽  
Vol 88 (1) ◽  
pp. 83-83 ◽  
Author(s):  
M. Truter ◽  
F. C. Wehner
Keyword(s):  
South Africa ◽  
Rhizoctonia Solani ◽  
Rating Scale ◽  
Solanum Tuberosum L ◽  
Potato Cultivar ◽  
Stem Canker ◽  
Potato Production ◽  
Black Scurf ◽  
Chi Square ◽  
Chi Square Analysis

Rhizoctonia disease (black scurf of tubers and stem canker) of potato (Solanum tuberosum L.) caused by Rhizoctonia solani Kühn was first recorded in South Africa in 1918 (3). Although the sclerotial form on tubers is one of the most common potato diseases in the country, it is not known which anastomosis groups (AGs) of R. solani are involved. Between 1999 and 2001, R. solani was isolated from 28 plant and 56 soil samples collected in 7 (Eastern Free State, Gauteng, KwaZulu-Natal, Limpopo, Mpumalanga, Northern Cape, and Sandveld) of the 14 potato-production regions of South Africa and screened for hyphal anastomosis with tester strains of R. solani AG-1 to AG-10 according to Carling et al. (1). Of the 411 isolates from tubers with black scurf symptoms, 408 were AG-3 and three were AG-5. Symptomless tubers yielded two AG-3 isolates and three AG-5 isolates. Of 39 isolates from symptomatic stems and roots, 32 were AG-3, five were AG-4, and two were AG-5. Of the 127 isolates obtained from soil, 86, 28, 7, 3, and 3 were AG-3, AG-4, AG-5, AG-7, and AG-8, respectively. More than one AG was isolated from five of the seven regions. Virulence of 40 isolates representative of the above AGs was determined in triplicate on sprouts growing from seed tubers of potato cultivar Up-to-Date in a sand/soil mixture as described by Carling and Leiner (2) but using cultures grown in cornmeal/sand instead of colonized agar disks as inoculum. Damage to sprouts (lesions, girdling, and death) was assessed after 28 days at 16 to 28°C according to the 0 to 4 rating scale (2). Chi-square analysis of the data indicated that AG-3 was the most virulent, with isolates from sclerotia on tubers and lesions on stems more aggressive than those from symptomless tubers or soil. AG-4 and AG-5 caused significantly less disease than AG-3, but none of the AG-7 and AG-8 isolates showed any virulence to potato sprouts. References: (1) D. E. Carling et al. Phytopathology 77:1609, 1987. (2) D. E. Carling and R. H. Leiner. Phytopathology 80:930, 1990. (3) E. M. Doidge. S. Afr. Fruit Growers 5:6, 1918.

scholarly journals Anastomosis Groups and Pathogenicity of Rhizoctonia solani and Binucleate Rhizoctonia from Potato in South Africa

Plant Disease ◽  
2015 ◽  
Vol 99 (12) ◽  
pp. 1790-1802 ◽  
Author(s):  
N. Muzhinji ◽  
M. Truter ◽  
J. W. Woodhall ◽  
J. E. van der Waals
Keyword(s):  
South Africa ◽  
Rhizoctonia Solani ◽  
Stem Canker ◽  
Black Scurf ◽  
Bootstrap Support ◽  
Internal Transcribed Spacer Region ◽  
Binucleate Rhizoctonia ◽  
Anastomosis Groups ◽  
Potato Plants ◽  
Comprehensive Survey

A survey of anastomosis groups (AG) of Rhizoctonia spp. associated with potato diseases was conducted in South Africa. In total, 112 Rhizoctonia solani and 19 binucleate Rhizoctonia (BNR) isolates were recovered from diseased potato plants, characterized for AG and pathogenicity. The AG identity of the isolates was confirmed using phylogenetic analysis of the internal transcribed spacer region of ribosomal DNA. R. solani isolates recovered belonged to AG 3-PT, AG 2-2IIIB, AG 4HG-I, AG 4HG-III, and AG 5, while BNR isolates belonged to AG A and AG R, with frequencies of 74, 6.1, 2.3, 2.3, 0.8, 12.2, and 2.3%, respectively. R. solani AG 3-PT was the most predominant AG and occurred in all the potato-growing regions sampled, whereas the other AG occurred in distinct locations. Different AG grouped into distinct clades, with high maximum parsimony and maximum-likelihood bootstrap support for both R. solani and BNR. An experiment under greenhouse conditions with representative isolates from different AG showed differences in aggressiveness between and within AG. Isolates of AG 2-2IIIB, AG 4HG-III, and AG R were the most aggressive in causing stem canker while AG 3-PT, AG 5, and AG R caused black scurf. This is the first comprehensive survey of R. solani and BNR on potato in South Africa using a molecular-based approach. This is the first report of R. solani AG 2-2IIIB and AG 4 HG-I causing stem and stolon canker and BNR AG A and AG R causing stem canker and black scurf on potato in South Africa.

scholarly journals Formation of canker lesions on stems and black scurf on tubers in experimentally inoculated potato plants by isolates of AG2-1, AG3 and AG5 of Rhizoctonia solani: a pilot study and literature review

2008 ◽  
Vol 18 (3-4) ◽  
pp. 223 ◽  
Author(s):  
M.J. LEHTONEN ◽  
P.S. WILSON ◽  
P. AHVENNIEMI
Keyword(s):  
Pilot Study ◽  
Rhizoctonia Solani ◽  
Stem Canker ◽  
Potato Production ◽  
Anastomosis Group ◽  
Black Scurf ◽  
Potato Tubers ◽  
Potato Plants ◽  
Production Areas ◽  
Potato Crops

Development of black scurf on potato tubers (cv. Nicola) was compared in plants inoculated with isolates of Rhizoctonia solani of three anastomosis groups (AG2-1, AG3 and AG5) which occur in potato crops in Finland. All isolates induced stem canker lesions but only isolates of AG3 formed efficiently black scurf on progeny tubers. Among the AG2-1 and AG5 isolates tested, only one AG2-1 isolate formed a few sclerotia on 13.5 % of the progeny tubers in one experiment. The data indicate that isolates of AG3 differ from those of AG2-1 and AG5 in having a higher ability to form sclerotia on tubers. Therefore, while AG2-1 and AG5 isolates have a broader host range, AG3 is more efficient in producing black scurf, which provides this anastomosis group with more efficient means of dissemination on seed potatoes. These differences probably explain the predominance of AG3 (98.9 % of isolates) in potato crops in Finland and other northern potato production areas.;

scholarly journals First Report of Rhizoctonia solani AG 4HG-III Causing Potato Stem Canker in South Africa

Plant Disease ◽  
2014 ◽  
Vol 98 (6) ◽  
pp. 853-853 ◽  
Author(s):  
N. Muzhinji ◽  
J. W. Woodhall ◽  
M. Truter ◽  
J. E. van der Waals
Keyword(s):  
South Africa ◽  
Rhizoctonia Solani ◽  
Stem Canker ◽  
Black Scurf ◽  
Limpopo Province ◽  
Control Treatment ◽  
First Report ◽  
Potato Diseases ◽  
Plant Pathol ◽  
Barley Grains

Black scurf and stem canker caused by Rhizoctonia solani Kühn (teleomorph: Thanathephorus cucumeris Frank Donk) are potato diseases of worldwide economic importance (4). R. solani consists of 13 anastomosis groups (AGs) of which AG 3-PT is considered the dominant causal agent of potato diseases globally (1,4). However, other AGs such as AG 2-1, 5, and 8 have been reported to cause potato diseases (1,4). In February 2013, potato stem samples (cv. Mondial) displaying dark brown lesions resembling those caused by Rhizoctonia stem canker were obtained from a commercial field in Limpopo Province, South Africa. Symptomatic tissue was disinfected with 1% NaOCl for 1 min, rinsed in sterile water, and 4-mm stem pieces excised from the margins of symptomatic tissues and plated on 2% water agar supplemented with 20 mg/l of chloramphenicol. Single hyphal tips taken from fungal isolates identified as R. solani based on morphological traits (3) were transferred to potato dextrose agar. DNA was isolated from the resulting cultures and ITS region of rDNA was sequenced as previously described (2). The resulting sequences of three of the isolates, Rh 81, Rh 82, and Rh 83 (KF712285, KF712286, and KF712287), were 99% similar to those of AG 4 HG-III found in GenBank (DQ102449 and AF354077). Therefore, based on molecular methods, these three isolates were identified as R. solani AG4 HG-III. To determine pathogenicity of the AG4 HG-III isolates, certified disease free mini-tubers (Generation 0, cv. Mondial, produced in tunnels) were used in pot trials. PDA plugs of each isolate were added to 10 g of barley grains, which had been sterilized by autoclaving for two consecutive days at 121°C for 30 min, and were incubated for 14 days until fully colonized. Ten colonized barley grains were placed 10 mm above each mini-tuber planted in 5l pots containing sterile potting mixture of sand:clay:pinebark (1:1:1). Ten tubers were inoculated with each isolate. Uninoculated, sterile barley grains were applied to the control treatment. Mini-tubers were grown in a greenhouse maintained at 22°C with light for a 12 h day. After 7 weeks, five plants for each isolate were destructively sampled and assessed for stem canker symptoms. At 120 days after sowing, the remaining five plants per treatment were assessed for blemishes on progeny tubers. The stem canker incidences of plants inoculated with Rh 81, Rh 82, and Rh 83 were 25, 25, and 50%, respectively, whereas no symptoms were observed in control plants. Sclerotia formation and blemishes were not observed on any of the progeny tubers, which might indicate that these strains are only able to infect stems, or that environmental conditions were not suitable for tuber blemish or black scurf development. R. solani AG4 HG-III was consistently re-isolated from symptomatic stems displaying brown lesions, and the identity of the re-isolates were confirmed by molecular tests as previously described, thereby fulfilling Koch's postulates. To our knowledge, this is the first report of R. solani AG4 HG-III causing stem canker on potato in South Africa and worldwide. Knowledge of which AGs are present in crop production systems is important when considering disease management strategies such as crop rotation and fungicide treatments (3). References: (1) C. Campion et al. Eur. J. Plant. Pathol. 109:983, 2003. (2) N. Muzhinji et al. Plant Dis. 98:570, 2014. (3) L. Tsror. J. Phytopathol. 158:649, 2010. (4) J. W. Woodhall et al. Plant. Pathol. 56:286, 2007.

scholarly journals Relative Contribution of Seed Tuber- and Soilborne Inoculum to Potato Disease Development and Changes in the Population Genetic Structure of Rhizoctonia solani AG 3-PT under Field Conditions in South Africa

Plant Disease ◽  
2018 ◽  
Vol 102 (1) ◽  
pp. 60-66 ◽  
Author(s):  
Norman Muzhinji ◽  
James W. Woodhall ◽  
Mariette Truter ◽  
Jacquie E. van der Waals
Keyword(s):  
South Africa ◽  
Rhizoctonia Solani ◽  
Gene Diversity ◽  
Seed Tuber ◽  
Management Strategies ◽  
Stem Canker ◽  
Molecular Techniques ◽  
Tuber Initiation ◽  
Fixation Index ◽  
Potato Disease

Understanding the contribution of seed tuber- and soilborne inocula of Rhizoctonia solani AG 3-PT in causing potato disease epidemics is an important step in implementing effective management strategies for the pathogen. A 2-year study was conducted to evaluate the contribution of each source of inoculum using an integrative experimental approach combining field trials and molecular techniques. Two distinct sets of genetically marked isolates were used as seed tuberborne and soilborne inocula in a mark-release-recapture experiment. Disease assessments were done during tuber initiation and at tuber harvest. Both inoculum sources were found to be equally important in causing black scurf disease, whereas soilborne inocula appeared to be more important for root and stolon infection, and seedborne inocula contributed more to stem canker. However, seed tuber-transmitted genotypes accounted for 60% of the total recovered isolates when genotyped using three polymerase chain reaction restriction fragment length polymorphism markers. The changes in population structure of the experimental R. solani population over the course of the growing season and across two growing seasons were investigated using eight microsatellite markers. The populations at different sampling times were somewhat genetically differentiated, as indicated by Nei’s gene diversity (0.24 to 0.27) and the fixation index (FST). The proportion of isolates with genotypes that differed from the inoculants ranged from 13 to 16% in 2013 and 2014, respectively, suggesting the possibility of emergence of new genotypes in the field. Because both soilborne and tuberborne inocula are critical, it is important to ensure the use of pathogen-free seed tubers to eliminate seed tuberborne inoculum and the introduction of new genotypes of R. solani for sustainable potato production in South Africa.

scholarly journals Hubungan lamanya hemodialisis dengan tingkat depresi pada pasangan hidup pasien gagal ginjal kronik di RSUP Prof. Dr. R. D. Kandou Manado

e-CliniC ◽  
2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Vicka V. A. Tartum ◽  
Theresia M. D. Kaunang ◽  
Christofel Elim ◽  
Neni Ekawardani
Keyword(s):  
Rating Scale ◽  
Chronic Renal Disease ◽  
Chronic Kidney Failure ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Loved One ◽  
Hamilton Depression Rating Scale ◽  
Chi Square Analysis ◽  
The Impact

Abstract: Depression is a disturbance in the function of human nature associated with feelings of sadness and accompanying symptoms, including changes in sleep patterns and appetite, anhedonia, concentration, psychomotor, fatigue, despair and helplessness. Depression can be obtained by any person, including a person suffering from chronic diseases such as chronic renal disease requiring hemodialysis therapy for life. However, the impact of depression was not only felt by the patient, but also the patient's family, especially spouses of patients would be very easy to get depressed as a result of seeing a loved one suffer, so will affect the support and motivation given to patients, especially in patients undergoing hemodialysis should undergo dialysis for life, so a lot of depression in patients and their families, especially spouses of patients. This study aimed to determine the relationship of the length of hemodialysis with the level of depression in spouses of hemodialysis patients in Prof. Dr. R. D. Kandou Hospital Manado. This was an observational analystical study with a cross sectional design. This study used Hamilton Depression Rating Scale questionaire (HDRS). Data were processed by using SPSS 20.0. The chi-square analysis test showed a p value of 0.105 (p > 0.05). Conclusion: There was no correlation between the duration of hemodialysis with the degree of depression of chronic kidney failure patient’s live spouses.Keywords: depression, chronic kidney disease, hemodialysis, spouse.Abstrak: Depresi adalah terganggunya fungsi manusia yang berkaitan dengan alam perasaan yang sedih dan gejala penyertanya, termasuk perubahan pada pola tidur dan nafsu makan, anhedonia, konsentrasi, psikomotor, kelelahan rasa putus asa dan tidak berdaya. Depresi bisa didapatkan oleh setiap orang termasuk seseorang yang menderita penyakit kronik seperti penyakit ginjal kronik yang membutuhkan terapi hemodialisis seumur hidup. Dampak depresi pun tidak hanya dirasakan oleh pasien, keluarga pasien terutama pasangan hidup pasien akan sangat mudah mendapatkan depresi akibat melihat orang yang dicintai menderita, sehingga akan memengaruhi dukungan dan motivasi yang akan diberikan kepada pasien, terutama pada pasien yang menjalani hemodialisis yang harus menjalani proses cuci darah seumur hidup, sehingga banyak terjadi depresi pada pasien dan keluarganya terutama pasangan hidup pasien. Penelitian ini bertujuan untuk mengetahui hubungan lamanya hemodialisis dengan tingkat depresi pada pasangan hidup pasien hemodialisis di RSUP Prof. Dr. R. D. Kandou Manado. Metode penelitian yang digunakan ialah analitik observasional dengan pendekatan potong lintang. Penelitian ini menggunakan kuesioner Hamilton Depression Rating Scale (HDRS) dan data diolah menggunakan SPSS 20.0. Uji analisis chi-square mendapatkan nilai p sebesar 0,105 (p >0,05). Simpulan: Tidak terdapat hubungan antara lama hemodialisis dengan tingkat depresi pasangan hidup pasien penyakit ginjal kronik.Kata kunci: depresi, penyakit ginjal kronik, hemodialisis, pasangan hidup

scholarly journals First Report of Rhizoctonia solani AG4 HG-II Infecting Potato Stems in Idaho

Plant Disease ◽  
2012 ◽  
Vol 96 (11) ◽  
pp. 1701-1701 ◽  
Author(s):  
J. W. Woodhall ◽  
P. S. Wharton ◽  
J. C. Peters
Keyword(s):  
Rhizoctonia Solani ◽  
Seed Tuber ◽  
Its Region ◽  
Stem Canker ◽  
Potato Production ◽  
Koch’S Postulates ◽  
First Report ◽  
Rdna Its ◽  
Plant Pathol ◽  
Koch's Postulates

The fungus Rhizoctonia solani is the causal agent of stem canker and black scurf of potato (Solanum tuberosum). R. solani is a species complex consisting of 13 anastomosis groups (AGs) designated AG1 to 13 (2, 3). Stems of potato (cv. Russet Norkotah) with brown lesions were recovered from one field in Kimberley, Idaho, in August 2011. Using previously described methods (3), R. solani was recovered from the symptomatic stems and one representative isolate (J15) was selected for further characterization. Sequencing of the rDNA ITS region of isolate J15 was undertaken as previously described (3) and the resulting rDNA ITS sequence (HE667745) was 99% identical to sequences of other AG4 HG-II isolates in GenBank (AF354072 and AF354074). Pathogenicity of the isolate was determined by conducting the following experiment. Mini-tubers of cv. Santé were planted individually in 1-liter pots containing John Innes Number 3 compost (John Innes Manufacturers Association, Reading, UK). Pots were either inoculated with J15, an isolate of AG3-PT (Rs08), or were not inoculated. Each treatment was replicated four times. Inoculum consisted of five 10-mm-diameter potato dextrose agar plugs, fully colonized by the appropriate isolate, placed in the compost approximately 40 mm above each seed tuber. Pots were held in a controlled environment room at 21°C with 50% relative humidity and watered as required. After 21 days, plants were assessed for disease. No symptoms of the disease were present in non-inoculated plants. In the Rs08 (AG3-PT) inoculated plants, all stems displayed large brown lesions and 20% of the stems had been killed. No stem death was observed in J15 (AG4 HG-II) inoculated plants. However, brown lesions were observed in three of the four J15 (AG4 HG-II) inoculated plants. These lesions were less severe than in plants inoculated with the Rs08(AG3-PT) inoculated plants and were present in 40% of the main stems. In the J15 (AG4 HG-II) inoculated pots, R. solani AG4 HG-II was reisolated from the five symptomatic stems, thereby satisfying Koch's postulates. To our knowledge, this is the first report of AG4 HG-II causing disease on potatoes in Idaho. AG4 has been isolated from potato previously from North Dakota, although the subgroup was not identified (1). The only previous report where AG4 HG-II was specifically determined to cause disease on potato was in Finland, but the isolate could not be maintained and Koch's postulates were not completed (3). The present study shows that AG4 HG-II can cause stem disease in potatoes, although disease does not develop as severely or as consistently as for AG3-PT. However, as demonstrated with isolates of AG2-1 and AG5, even mild stem infection can reduce tuber yield by as much as 12% (4). AG4 HG-II is a pathogen of sugar beet in Idaho, which was grown previously in this field. This history may have contributed to high levels of soilborne inoculum required to produce disease on potato. References: (1) N. C. Gudmestad et al. Page 247 in: J. Vos et al. eds. Effects of Crop Rotation on Potato Production in the Temperate Zones. Kluwer, Dordrecht, Netherlands, 1989. (2) M. J. Lehtonen et al. Agric. Food Sci. 18:223, 2009. (3) J. W. Woodhall et al. Plant Pathol. 56:286, 2007. (4) J. W. Woodhall et al. Plant Pathol. 57:897, 2008.

scholarly journals In vitro evaluation of fungicidal responses on the growth of pathogenic Rhizoctonia solani Kuhn, antagonistic binucleate Rhizoctonia and Trichoderma harzianum Rifai

1970 ◽  
Vol 39 (1) ◽  
pp. 107-110
Author(s):  
Md Maniruzzaman Khandaker ◽  
Md Khurshed Alam Bhuiyan ◽  
Abul Khair
Keyword(s):  
Boric Acid ◽  
Rhizoctonia Solani ◽  
Key Words ◽  
Trichoderma Harzianum ◽  
Stem Canker ◽  
Black Scurf ◽  
Binucleate Rhizoctonia ◽  
Crop Fields ◽  
Potato Plants

Two pathogenic isolates of Rhizoctonia solani Kuhn causing stem canker/black scurf disease of potato plants and four antagonist isolates, two of binucleate Rhizoctonia and two of Trichoderma harzianum Rifai were isolated from crop fields and evaluated in vitro for their fungicidal responses against eight fungicides. Vitavax was effective in inhibiting the growth of R. solani and binucleate Rhizoctonia but it did not inhibit the growth of T. harzianum at 100 ppm concentration. Terraclor Super X, Dithane M 45 and Boric acid are the fungicides which at 100 ppm concentration did not inhibit the growth of antagonist isolates of T. harzianum and binucleate Rhizoctonia but inhibited the growth of isolates of R. solani to some extent. The in vitro findings suggest that any one of these three fungicides along with antagonist isolates of binucleate Rhizoctonia and T. harzianum can be used as biocontrol agents to reduce soil borne inocula of R. solani. Key words: Rhizoctonia solani; Binucleate; Trichoderma harzianum; Fungicide DOI: 10.3329/bjb.v39i1.5534Bangladesh J. Bot. 39(1): 107-110, 2010 (June)

scholarly journals 1094 Depression Is Not Associated With The Presence Of Or The Severity Of Obstructive Sleep Apnea: Retrospective Study Of 841 Polysomnography Subjects

SLEEP ◽  
2020 ◽  
Vol 43 (Supplement_1) ◽  
pp. A416-A416
Author(s):  
K Im ◽  
L Kim ◽  
R Immen
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Rating Scale ◽  
Numerical Rating Scale ◽  
Significant Proportion ◽  
Chi Square ◽  
Relative Paucity ◽  
Obstructive Sleep ◽  
Significant Difference ◽  
Chi Square Analysis

Abstract Introduction Both depression and obstructive sleep apnea (OSA) are very common medical conditions. Studies showed a co-occurrence of depression and OSA with a higher prevalence of one if the other is present. However, there is relative paucity of studies assessing the rate of depression based on the OSA severity. Methods Retrospective analysis of data collected from patients undergoing polysomnography (PSG) at an academic sleep disorders center was performed. A total of 841 subjects were included and stratified into four groups using AHI. A Chi-square analysis was applied to assess the association of varying levels of AHI and the presence of depression. Results Although a significant proportion of patients with AHI greater than 5 endorsed depression (60/165 in group with AHI 15 or greater and 115/278 in group with AHI between 5 and 15), this finding was also replicated in patients with AHI less than 5 (86/202 in AHI between 1 and 5 and 88/196 in those with AHI less than 1). As there was significant difference in rate of depression among women (54.1%) and men (26.1%) (p <0.0001), Chi-square analysis was performed for the rate of depression based on the level of AHI, adjusted for gender. In women the rate of depression from the most severe AHI to less severe AHI group were 0.48, 0.53, 0.60, and 0.53 respectively and in men it was 0.30, 0.27, 0.20, and 0.27 respectively, with no statistical difference between any groups. Conclusion Among patients who seek PSG assessment, depression appears to be more prevalent than the general public. Rate of depression is much higher among women than men in this group. However, the presence of OSA or severity of OSA does not have any correlation with the rate of depression in both women and men. These findings might be suggestive of the complexity of the association between depression and OSA. One limitation of this study is the dichotomous nature of depression (presence or absence of). The finding from this study warrants a future study utilizing a numerical rating scale of depression for severity measure to correlate it with the severity of OSA. Support NA.

scholarly journals Accuracy of ultrasound-guided versus palpation-based carpometacarpal joint injections: A randomized pilot study in cadavers

Ultrasound ◽  
2018 ◽  
Vol 26 (4) ◽  
pp. 245-250
Author(s):  
Armen Derian ◽  
Julia Amundson ◽  
Karl Abi-Aad ◽  
Ricardo Vasquez-Duarte ◽  
Douglas Johnson-Greene
Keyword(s):  
Ultrasound Guidance ◽  
Rating Scale ◽  
Carpometacarpal Joint ◽  
Ultrasound Guided ◽  
Chi Square ◽  
Rater Reliability ◽  
Carpometacarpal Joints ◽  
Significant Difference ◽  
Chi Square Analysis ◽  
Joint Injections

Objective To determine the accuracy of ultrasound guidance compared to palpation in performing carpometacarpal joint injections in cadavers. Design In all, 36 carpometacarpal joints were randomized to either ultrasound-guided or palpation-based injections, with 1 cc of blue latex solution injected into each joint. The specimens were then dissected and the distribution of the latex was assessed by two independent, blinded raters. Injection accuracy was evaluated on a four-point quartile rating scale of 1–4, corresponding to the amount of the latex solution within the joint (1 = 0–25%, 2 = 26–50%, 3 = 51–75%, 4 = 76–100%). Inter-rater reliability was a secondary measure. Results The mean rating of accuracy was 2.1 for both palpation-based and ultrasound-guided injections. There was no statistically significant difference in accuracy between the two injectors. Chi-square analysis testing differences in accuracy for the two conditions was not statistically significant. The Cronbach’s alpha for rater 2 was 0.74, which represents an acceptable level of reliability. A Friedman’s Chi-square for the two raters was 2.3 ( p = 0.13), indicating no significant difference between raters. Conclusion Ultrasound guidance did not improve the accuracy of carpometacarpal joint injections in cadavers. However, the high inter-rater reliability attests to the value of the novel assessment scale.

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