Morphological and Molecular Characterization of Sclerotinia sclerotiorum on Sugar Beet in Montana, USA

2021 ◽  
Author(s):  
Mohamed F. R Khan ◽  
Md. Ziaur Rahman Bhuyian ◽  
Dilip Lashman ◽  
Presley Mosher ◽  
Timothy Cymbaluk ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Sugar Beet ◽  
Sclerotinia Sclerotiorum ◽  
Internal Transcribed Spacer ◽  
Economic Activities ◽  
Internal Transcribed Spacer Region ◽  
Molecular Identity ◽  
South Central ◽  
Morphological And Molecular Characterization

In this contribution, we report on a survey of leaf blight on sugar beet in the Sidney factory district of Montana caused by the soilborne fungus Sclerotinia sclerotiorum. In addition to morphological and pathological characterization of the pathogen on sugar beet, we confirmed the molecular identity of the fungus by DNA sequence analysis of the ribosomal Internal Transcribed Spacer region. Our findings are significant because the sugar beet production in south-central and eastern Montana contributes more than $100 million in economic activities. The incidence of this emerging disease will require changes in conventional disease management.

scholarly journals Overview of Agaricus Subrufescens Strains used in the Last 15 Years in Brazil and other Countries and Current Potential Materials for the Future

2021 ◽  
Author(s):  
Diego Cunha Zied ◽  
Wagner G. Vieira Junior ◽  
Douglas M. M. Soares ◽  
Cassius V. Stevani ◽  
Eustáquio S. Dias ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
High Weight ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Agaricus Blazei ◽  
Internal Transcribed Spacer Region ◽  
Commercial Use ◽  
Agaricus Subrufescens ◽  
Current Potential

Abstract The mushroom Agaricus subrufescens has been synonymous with Agaricus blazei and Agaricus brasiliensis during the last decades and there has been much discussion with regards to the origin, distribution, and nomenclature of this mushroom. Therefore, we conducted a genetic and morphological characterization of the mycelium and mushroom of four commercial strains currently cultivated in Brazil (ABL CS7, ABL 18/01, ABL 98/11, and ABL 16/01) together with an assessment of their agronomic behavior and compared these results with those of other strains used during the last 15 years. All the A. subrufescens strains characterized here are phylogenetically related to the Americas/Europe specimens, bearing an internal transcribed spacer region of type A (ABL 16/01) or both types A and B (ABL 18/01, ABL 98/11, and ABL CS7). We did not find any correlation between the morphological characteristics of the mycelial colonies and the agronomic behavior of the strains. Strains ABL 98/11 and ABL 16/01 produced the best yields and morphological characteristics for the mushrooms, indicating their high weight, which enhances the commercialization of the mushroom and justifies their longstanding commercial use over the last 15 years.

First Report of Leaf Blight of Sugar Beet (Beta vulgaris) Caused by Sclerotinia sclerotiorum in Minnesota, U.S.A.

2021 ◽  
pp. PHP-02-21-0048-
Author(s):  
Mohamed F. R. Khan ◽  
Md. Ziaur Rahman Bhuiyan ◽  
Yangxi Liu ◽  
Dilip Lakshman ◽  
Mark Bloomquist
Keyword(s):  
United States ◽  
Sugar Beet ◽  
Beta Vulgaris ◽  
Sclerotinia Sclerotiorum ◽  
Internal Transcribed Spacer ◽  
Economic Loss ◽  
The United States ◽  
Morphological Data ◽  
Varietal Resistance ◽  
First Time

Minnesota is the top sugar beet (Beta vulgaris L.) producing state in the United States. In 2020, sugar beet plants were observed for the first time in which the two to three oldest leaves had light brown to dark brown necrotic leaf lesions that eventually became yellow or brown and died but remained attached to the plant. Morphological data and sequences of internal transcribed spacer regions identified the pathogen as Sclerotinia sclerotiorum. Because over 90% of the plants in identified fields were infected it was difficult to quantify loss in yield or quality caused by this disease. All fields with symptomatic plants had soybean or edible beans in the rotation. One field planted to several different varieties indicated that all the varieties were symptomatic. It will be useful to determine any economic loss caused by S. sclerotiorum and any known varietal resistance to this pathogen.

Morphological and molecular characterization of Raphidascaris (Ichthyascaris) lophii (Wu, 1949) (Nematoda, Anisakidae) from marine fishes from China, with a key to the species of the subgenus Ichthyascaris

2012 ◽  
Vol 57 (3) ◽  
Author(s):  
Zhen Xu ◽  
Lu-Ping Zhang ◽  
Bao-Chen Liu ◽  
Liang Li
Keyword(s):  
South China Sea ◽  
South China ◽  
Internal Transcribed Spacer ◽  
Marine Fishes ◽  
Accurate Diagnosis ◽  
Target Sequence ◽  
Nucleotide Polymorphisms ◽  
Morphological And Molecular Characterization ◽  
Zeus Faber

AbstractThe little known ascaridoid nematode Raphidascaris (Ichthyascaris) lophii (Wu, 1949) is redescribed and illustrated based on newly collected specimens from the five different marine fishes: Lophius litulon (Jordan) (Lophiiformes: Lophiidae), Lophiomus setigerus (Vahl) (Lophiiformes: Lophiidae), Antennarius hispidus (Bloch et Schneider) (Lophiiformes: Antennaridae), Zeus faber Linnaeus (Zeiformes: Zeidae) and Ostichthys japonicus (Cuvier) (Beryciformes: Holocentridae) from the East and South China Sea. This species differs from all congeners in the subgenus Ichthyascaris by the length of the ventricular appendix (0.52–0.98 mm long), the number and arrangement of caudal papillae (26–32 pairs of precloacal, 3–4 pairs of paracloacal and 8–11 pairs of postcloacal) and the length of the spicules (0.49–0.88 mm long, representing 3.08–4.70% of body length). In addition, nematodes collected from these five different fishes have been characterized using molecular methods by sequencing and analysing the internal transcribed spacer (ITS) of ribosomal DNA. No variation in size and nucleotide polymorphisms is detected within the target sequence among all samples analysed. These data contribute to facilitate an accurate diagnosis of this poorly known nematode. An identification key to the species of the subgenus Ichthyascaris is also provided.

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