Isolation and Molecular Characterization of Bovine Trichophyton verrucosum Strains Based on Sequence Analysis of Internal Transcribed Spacer Region (ITS1) and Microsatellite Loci

In this contribution, we report on a survey of leaf blight on sugar beet in the Sidney factory district of Montana caused by the soilborne fungus Sclerotinia sclerotiorum. In addition to morphological and pathological characterization of the pathogen on sugar beet, we confirmed the molecular identity of the fungus by DNA sequence analysis of the ribosomal Internal Transcribed Spacer region. Our findings are significant because the sugar beet production in south-central and eastern Montana contributes more than $100 million in economic activities. The incidence of this emerging disease will require changes in conventional disease management.

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Internal Transcribed Spacer (ITS) Region Targeted Molecular Characterization of Macroalgal Diversity Along the Overlooked Expanse of Gulf of Kachchh, India

Proceedings of the National Academy of Sciences India Section B Biological Sciences ◽

10.1007/s40011-021-01273-8 ◽

2021 ◽

Author(s):

Dhara Dixit ◽

Nikunj Balar ◽

Mrugesh Trivedi ◽

Poornima Suthar ◽

C. R. K. Reddy ◽

...

Keyword(s):

Molecular Characterization ◽

Internal Transcribed Spacer ◽

Its Region ◽

Gulf Of Kachchh

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Sequence analysis and molecular characterization of genes required for the biosynthesis of type 1 capsular polysaccharide in Staphylococcus aureus.

Journal of Bacteriology ◽

10.1128/jb.176.22.7005-7016.1994 ◽

1994 ◽

Vol 176 (22) ◽

pp. 7005-7016 ◽

Cited By ~ 100

Author(s):

W S Lin ◽

T Cunneen ◽

C Y Lee

Keyword(s):

Staphylococcus Aureus ◽

Sequence Analysis ◽

Molecular Characterization ◽

Capsular Polysaccharide

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Molecular Characterization of Two Lactate Dehydrogenase Genes with a Novel Structural Organization on the Genome of Lactobacillus sp. Strain MONT4

Applied and Environmental Microbiology ◽

10.1128/aem.70.10.6290-6295.2004 ◽

2004 ◽

Vol 70 (10) ◽

pp. 6290-6295 ◽

Cited By ~ 5

Author(s):

Jennifer Weekes ◽

Gülhan Ü. Yüksel

Keyword(s):

Escherichia Coli ◽

Lactate Dehydrogenase ◽

Sequence Analysis ◽

Molecular Characterization ◽

Northern Blot ◽

Structural Organization ◽

Genomic Organization ◽

The Individual

ABSTRACT Two lactate dehydrogenase (ldh) genes from Lactobacillus sp. strain MONT4 were cloned by complementation in Escherichia coli DC1368 (ldh pfl) and were sequenced. The sequence analysis revealed a novel genomic organization of the ldh genes. Subcloning of the individual ldh genes and their Northern blot analyses indicated that the genes are monocistronic.

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Overview of Agaricus Subrufescens Strains used in the Last 15 Years in Brazil and other Countries and Current Potential Materials for the Future

10.21203/rs.3.rs-310100/v1 ◽

2021 ◽

Author(s):

Diego Cunha Zied ◽

Wagner G. Vieira Junior ◽

Douglas M. M. Soares ◽

Cassius V. Stevani ◽

Eustáquio S. Dias ◽

...

Keyword(s):

Internal Transcribed Spacer ◽

High Weight ◽

Morphological Characteristics ◽

Morphological Characterization ◽

Agaricus Blazei ◽

Internal Transcribed Spacer Region ◽

Commercial Use ◽

Agaricus Subrufescens ◽

Current Potential

Abstract The mushroom Agaricus subrufescens has been synonymous with Agaricus blazei and Agaricus brasiliensis during the last decades and there has been much discussion with regards to the origin, distribution, and nomenclature of this mushroom. Therefore, we conducted a genetic and morphological characterization of the mycelium and mushroom of four commercial strains currently cultivated in Brazil (ABL CS7, ABL 18/01, ABL 98/11, and ABL 16/01) together with an assessment of their agronomic behavior and compared these results with those of other strains used during the last 15 years. All the A. subrufescens strains characterized here are phylogenetically related to the Americas/Europe specimens, bearing an internal transcribed spacer region of type A (ABL 16/01) or both types A and B (ABL 18/01, ABL 98/11, and ABL CS7). We did not find any correlation between the morphological characteristics of the mycelial colonies and the agronomic behavior of the strains. Strains ABL 98/11 and ABL 16/01 produced the best yields and morphological characteristics for the mushrooms, indicating their high weight, which enhances the commercialization of the mushroom and justifies their longstanding commercial use over the last 15 years.

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