Molecular Mapping of YrSP and Its Relationship with Other Genes for Stripe Rust Resistance in Wheat Chromosome 2BL

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important disease of wheat worldwide. Resistance is the best way to control the disease. YrSP, a gene originally from ‘Spaldings Prolific’ wheat and providing resistance to a broad spectrum of races, is used for differentiating P. striiformis f. sp. tritici races but its chromosomal location is not clear. To map YrSP, a near-isogenic line (AvSYrSPNIL) was backcrossed to the recurrent parent, Avocet S. Genetic analysis of the BC7F1, BC8, BC7F2, and BC7F3 progenies confirmed a single dominant gene for resistance. In total, 182 BC7F2 plants and their derived BC7F3 lines were phenotyped with an avirulent P. striiformis f. sp. tritici race and genotyped with simple-sequence repeat (SSR), single-nucleotide polymorphism (SNP), and sequence-tagged site (STS) markers. A linkage map was constructed with 3 SSR, 17 SNP, and 3 STS markers covering 23.3 centimorgans (cM). Markers IWA638 and dp269 were 0.6 cM proximal and 1.5 cM distal, respectively, to YrSP. The gene was mapped in chromosome bin 2BL-C-0.5, physically within the proximal 50% of the chromosome 2BL arm. Allelism tests based on F2 phenotypes indicated that YrSP is closely linked to but not allelic with genes Yr5, Yr7, Yr43, Yr44, and Yr53. Infection type data from tests with 10 historical and currently predominant P. striiformis f. sp. tritici races in the United States also demonstrated differences in specificity between YrSP and the other genes. The specificity of YrSP is useful in differentiating P. striiformis f. sp. tritici races and studying the plant–pathogen interactions, and the information of chromosomal location of the gene and its tightly linked markers should be useful in developing resistant cultivars when combined with other genes for resistance to stripe rust.

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Molecular Mapping of Stripe Rust Resistance Gene Yr76 in Winter Club Wheat Cultivar Tyee

Phytopathology ◽

10.1094/phyto-01-16-0045-fi ◽

2016 ◽

Vol 106 (10) ◽

pp. 1186-1193 ◽

Cited By ~ 28

Author(s):

C. Xiang ◽

J. Y. Feng ◽

M. N. Wang ◽

X. M. Chen ◽

D. R. See ◽

...

Keyword(s):

Ssr Markers ◽

Stripe Rust ◽

Molecular Mapping ◽

Mapping Population ◽

Puccinia Striiformis ◽

Single Gene ◽

Dominant Gene ◽

The United States ◽

Stripe Rust Resistance ◽

Isogenic Line

Tyee, one of the wheat cultivars used to differentiate races of Puccinia striiformis f. sp. tritici in the United States, was identified to have a single gene for all-stage resistance, tentatively named YrTye. To map the gene, Tyee was crossed with ‘Avocet Susceptible’ (AvS). Genetic analysis of the F1, F2, F2:3, and BC1 progenies confirmed a single dominant gene for resistance to race PSTv-37 that is avirulent to YrTye. A mapping population of 135 F2 plants was phenotyped with PSTv-37 and the derived F2:3 lines were tested with races PSTv-37, PSTv-40, and PSTv-79. The F2 mapping population was genotyped with simple sequence repeat (SSR) markers. A genetic map comprising 13 SSR markers located YrTye in chromosome 3AS flanked distally by SSR marker wmc11 and proximally by wmc532 at 2.6 and 3.4 cM, respectively. Amplification of Chinese Spring 3A deletion lines placed the gene in the distal bin 3AS4-0.45 to 1.00. Because YrTye is different from all formally named Yr genes in chromosomal location, we permanently name the gene Yr76. A near-isogenic line of spring common wheat was developed and selected by testing F3 lines derived from a AvS*4/Tyee cross with Tyee-avirulent and virulent races and the flanking markers. The specific SSR alleles flanking Yr76 were validated using cultivars and breeding lines with and without the gene, and showed high polymorphisms. The specificity of Yr76 is useful in differentiating P. striiformis f. sp. tritici races, and its tightly linked markers will be useful in developing resistant cultivars when combining the gene with other genes for resistance to stripe rust.

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Molecular Mapping of a Stripe Rust Resistance Gene in Spring Wheat Cultivar Zak

Phytopathology ◽

10.1094/phyto-99-10-1209 ◽

2009 ◽

Vol 99 (10) ◽

pp. 1209-1215 ◽

Cited By ~ 32

Author(s):

X. X. Sui ◽

M. N. Wang ◽

X. M. Chen

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Spring Wheat ◽

Rust Resistance ◽

Molecular Mapping ◽

Puccinia Striiformis ◽

Yellow Rust ◽

Dominant Gene ◽

The United States ◽

Stripe Rust Resistance

Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici, is one of the most devastating foliar diseases of wheat (Triticum aestivum) worldwide. Growing resistant cultivars is the best approach for control of the disease. Although the stripe rust resistance in spring wheat cv. Zak has been circumvented by a group of races of the pathogen predominant in the United States since 2000, the resistance genes in Zak were unknown. To identify and map the genes for resistance to stripe rust, Zak was crossed with susceptible wheat genotype ‘Avocet Susceptible’. Seedlings of the parents and F1, F2, and F3 progeny were tested with P. striiformis f. sp. tritici races PST-43 and PST-45 under controlled greenhouse conditions. Genetic analysis determined that Zak has a single dominant gene, designated as YrZak, conferring race-specific all-stage resistance. Resistance gene analog polymorphism (RGAP), simple sequence repeat (SSR), and sequence-tagged site (STS) techniques were used to identify molecular markers linked to YrZak. A linkage group of three RGAP, three SSR, and three STS markers was constructed for YrZak using 205 F3 lines. Amplification of the complete set of Chinese Spring nulli-tetrasomic lines with RGAP marker Xwgp102 indicated that YrZak is present on chromosome 2B. The three SSR markers further mapped YrZak to the long arm of chromosome 2B. Amplification of chromosome 2B deletion lines with SSR marker Xgwm501 further confirmed that YrZak is on chromosome 2BL. To determine the genetic distance between YrZak and Yr5, which also is present on chromosome 2BL, 300 F2 plants from cross Zak/Yr5 were tested with PST-43. Six susceptible plants were identified from the F2 population, indicating that YrZak and Yr5 are ≈42 centimorgans apart. The results of race reactions and chromosomal locations indicated that YrZak is different from previously identified genes for resistance to stripe rust. This gene should be useful in monitoring virulence changes in the pathogen population and in studying host–pathogen interactions.

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Molecular Mapping of the Stripe Rust Resistance Gene Yr69 on Wheat Chromosome 2AS

Plant Disease ◽

10.1094/pdis-05-15-0555-re ◽

2016 ◽

Vol 100 (8) ◽

pp. 1717-1724 ◽

Cited By ~ 15

Author(s):

Liyuan Hou ◽

Juqing Jia ◽

Xiaojun Zhang ◽

Xin Li ◽

Zujun Yang ◽

...

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Rust Resistance ◽

Expressed Sequence Tag ◽

Molecular Mapping ◽

Puccinia Striiformis ◽

Dominant Gene ◽

Introgression Line ◽

Stripe Rust Resistance ◽

Resistance Specificity

Wheat is one of the major food crops in the world. Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an economically important disease that affects wheat worldwide. The discovery of novel resistance genes and the deployment of effectively resistant cultivars are important for the ongoing control of wheat stripe rust and the maintenance of the agricultural productivity of wheat. CH7086, a new stripe rust-resistant wheat introgression line, was selected by crossing susceptible cultivars with the resistant Thinopyrum ponticum-derived partial amphiploid Xiaoyan 7430. The resistance of CH7086 is effective against all current Chinese P. striiformis f. sp. tritici races. CH7086 was crossed with the stripe rust-susceptible cultivars to develop F1, F2, F3, and BC1 populations for genetic analysis. Segregation in the F2 and BC1 populations and F2:3 lines were tested for resistance against the P. striiformis f. sp. tritici race CYR32. This test showed that CH7086 carries a single dominant gene for stripe rust resistance, which was temporarily designated YrCH86. The closest of the eight simple sequence repeat (SSR) and expressed sequence tag-SSR markers flanking the locus were X2AS33, which is 1.9 cM distal, and Xmag3807, which is 3.1 cM proximal. The resistance gene and its polymorphic markers were placed in deletion bin 2AS-0.78-1.00 using the ‘Chinese Spring’ nullisomic-tetrasomic, ditelosomic, and deletion lines. The tests of both allelism and resistance specificity suggested that the resistance gene found in CH7086 was not Yr17, which was the only current formally named Yr gene on chromosome 2AS. Thus, YrCH86 appeared to be a new locus and was permanently designated Yr69.

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Rapid identification of a stripe rust resistance gene YrXK in Chinese wheat line Xike01015 using specific locus amplified fragment (SLAF) sequencing

Plant Disease ◽

10.1094/pdis-12-20-2648-re ◽

2021 ◽

Author(s):

Cai Sun ◽

Yike Liu ◽

Qiang Li ◽

Baotong Wang ◽

Shuhui Chen ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Gene Annotation ◽

Dominant Gene ◽

Rapid Identification ◽

Wheat Breeding ◽

Stripe Rust Resistance ◽

Phenotypic Variance ◽

F2 Population ◽

Chinese Wheat

Wheat stripe rust, an airborne fungal disease and caused by Puccinia striiformis Westend. f. sp. tritici (Pst), is one of the most devastating diseases on wheat. It is the most effective and economical measure for the diseases control to identify high-level resistance genes and apply in wheat breeding. Chinese wheat cultivar Xike01015 presents high levels of all stage resistance (ASR) to the current predominant Pst race CYR33. In this study, a single dominant gene, designated as YrXk, was identified in Xike01015 conferring resistance to CYR33 with genetic analysis of F2 and BC1 population from cross of Mingxian169 (susceptible) and Xike01015. The specific length amplified fragment sequencing (SLAF-seq) strategy was used to construct linkage map in the F2 population. QTL analysis mapped YrXk to a 12.4 Mb segment on chromosome1BS, explaining over 86.96% phenotypic variance. Gene annotation in the QTL region identified three differential expressed candidate genes , TraesCS1B02G168600.1, TraesCS1B02G170200.1, and TraesCS1B02G172400.1. The qRT-PCR results displayed that TraesCS1B02G170200.1 and TraesCS1B02G168600.1 significantly up-regulated and down-regulated, respectively, and TraesCS1B02G170200.1 slightly up-regulated after changed with CYR33 in the seedling stage, which indicating these genes may function in wheat resistance to stripe rust. The results of this study can be used in wheat breeding for improving resistance to stripe rust.

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Inheritance and Molecular Mapping of Barley Genes Conferring Resistance to Wheat Stripe Rust

Phytopathology ◽

10.1094/phyto-95-0884 ◽

2005 ◽

Vol 95 (8) ◽

pp. 884-889 ◽

Cited By ~ 22

Author(s):

Vihanga Pahalawatta ◽

Xianming Chen

Keyword(s):

Resistance Gene ◽

Microsatellite Marker ◽

Stripe Rust ◽

Molecular Mapping ◽

Puccinia Striiformis ◽

Infection Type ◽

Dominant Gene ◽

Wheat Stripe Rust ◽

Rust Pathogen ◽

Type Data

Most barley cultivars are resistant to stripe rust of wheat that is caused by Puccinia striiformis f. sp. tritici. The barley cv. Steptoe is susceptible to all identified races of P. striiformis f. sp. hordei (PSH), the barley stripe rust pathogen, but is resistant to most P. striiformis f. sp. tritici races. To determine inheritance of the Steptoe resistance to P. striiformis f. sp. tritici, a cross was made between Steptoe and Russell, a barley cultivar susceptible to some P. striiformis f. sp. tritici races and all tested P. striiformis f. sp. hordei races. Seedlings of parents and F1, BC1, F2, and F3 progeny from the barley cross were tested with P. striiformis f. sp. tritici races PST-41 and PST-45 under controlled greenhouse conditions. Genetic analyses of infection type data showed that Steptoe had one dominant gene and one recessive gene (provisionally designated as RpstS1 and rpstS2, respectively) for resistance to races PST-41 and PST-45. Genomic DNA was extracted from the parents and 150 F2 plants that were tested for rust reaction and grown for seed of F3 lines. The infection type data and polymorphic markers identified using the resistance gene analog polymorphism (RGAP) technique were analyzed with the Mapmaker computer program to map the resistance genes. The dominant resistance gene in Steptoe for resistance to P. striiformis f. sp. tritici races was mapped on barley chromosome 4H using a linked microsatellite marker, HVM68. A linkage group for the dominant gene was constructed with 12 RGAP markers and the microsatellite marker. The results show that resistance in barley to the wheat stripe rust pathogen is qualitatively inherited. These genes might provide useful resistance against wheat stripe rust when introgressed into wheat from barley.

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Development of resistance gene analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust

Genome ◽

10.1139/g01-028 ◽

2001 ◽

Vol 44 (4) ◽

pp. 509-516 ◽

Cited By ~ 34

Author(s):

Z X Shi ◽

X M Chen ◽

R F Line ◽

H Leung ◽

C R Wellings

Keyword(s):

Molecular Markers ◽

Resistance Gene ◽

Stripe Rust ◽

Puccinia Striiformis ◽

Resistance Gene Analog ◽

Stripe Rust Resistance ◽

Isogenic Line ◽

Wheat Cultivars ◽

Development Of Resistance ◽

Rye Translocations

The Yr9 gene, which confers resistance to stripe rust caused by Puccinia striiformis f.sp. tritici (P. s. tritici) and originated from rye, is present in many wheat cultivars. To develop molecular markers for Yr9, a Yr9 near-isogenic line, near-isogenic lines with nine other Yr genes, and the recurrent wheat parent 'Avocet Susceptible' were evaluated for resistance in the seedling stage to North American P. s. tritici races under controlled temperature in the greenhouse. The resistance gene analog polymorphism (RGAP) technique was used to identify molecular markers for Yr9. The BC7:F2 and BC7:F3 progeny, which were developed by backcrossing the Yr9 donor wheat cultivar Clement with 'Avocet Susceptible', were evaluated for resistance to stripe rust races. Genomic DNA was extracted from 203 BC7:F2 plants and used for cosegregation analysis. Of 16 RGAP markers confirmed by cosegregation analysis, 4 were coincident with Yr9 and 12 were closely linked to Yr9 with a genetic distance ranging from 1 to 18 cM. Analyses of nulli-tetrasomic 'Chinese Spring' lines with the codominant RGAP marker Xwgp13 confirmed that the markers and Yr9 were located on chromosome 1B. Six wheat cultivars reported to have 1B/1R wheat-rye translocations and, presumably, Yr9, and two rye cultivars were inoculated with four races of P. s. tritici and tested with 9 of the 16 RGAP markers. Results of these tests indicate that 'Clement', 'Aurora', 'Lovrin 10', 'Lovrin 13', and 'Riebesel 47/51' have Yr9 and that 'Weique' does not have Yr9. The genetic information and molecular markers obtained from this study should be useful in cloning Yr9, in identifying germplasm that may have Yr9, and in using marker-assisted selection for combining Yr9 with other stripe rust resistance genes.Key words: molecular markers, Puccinia striiformis f.sp. tritici, resistance gene analog polymorphism, Triticum aestivum.

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Molecular Mapping of Stripe Rust Resistance Gene Yr81 in a Common Wheat Landrace Aus27430

Plant Disease ◽

10.1094/pdis-06-18-1055-re ◽

2019 ◽

Vol 103 (6) ◽

pp. 1166-1171 ◽

Cited By ~ 14

Author(s):

Mesfin Gessese ◽

Harbans Bariana ◽

Debbie Wong ◽

Matthew Hayden ◽

Urmil Bansal

Keyword(s):

Stripe Rust ◽

Rust Resistance ◽

Molecular Mapping ◽

Puccinia Striiformis ◽

Stripe Rust Resistance ◽

Chromosomal Assignment ◽

Resistance Locus ◽

Sources Of Resistance ◽

Stripe Rust Resistance Gene ◽

Ril Population

The deployment of diverse sources of resistance in new cultivars underpins durable control of rust diseases. Aus27430 exhibited a moderate level of stripe rust resistance against Puccinia striiformis f. sp. tritici (Pst) pathotypes currently prevalent in Australia. Aus27430 was crossed with the susceptible parent Avocet S (AvS) and subsequent filial generations were raised. Monogenic segregation observed among Aus27430/AvS F3 families was confirmed through stripe rust screening of an F6 recombinant inbred line (RIL) population, and the resistance locus was temporarily named YrAW5. Selective genotyping using an Illumina iSelect 90K wheat SNP bead chip array located YrAW5 in chromosome 6A. Genetic mapping of the RIL population with linked 90K SNPs that were converted into PCR-based marker assays, as well as SSR markers previously mapped to chromosome 6A, confirmed the chromosomal assignment for YrAW5. Comparative analysis of other stripe rust resistance genes located in chromosome 6A led to the formal designation of YrAW5 as Yr81. Tests with a marker linked with Yr18 also demonstrated the presence of this gene in Aus27430. Yr18 interacted with Yr81 to produce stripe rust responses lower than those produced by RILs carrying these genes individually. Although gwm459 showed higher recombination with Yr81 compared with the other flanking marker KASP_3077, it amplified the AvS allele in 80 cultivars, whereas KASP_3077 amplified AvS allele in 67 cultivars. Both markers can be used in marker-assisted selection after confirming parental polymorphism.

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Molecular identification and mapping of a novel stripe rust resistance gene in wheat resistance line CH5389

Ciência Rural ◽

10.1590/0103-8478cr20170846 ◽

2018 ◽

Vol 48 (5) ◽

Author(s):

Haixian Zhan ◽

Huijuan Guo ◽

Linyi Qiao ◽

Liping Mao ◽

Shuosheng Zhang

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Dominant Gene ◽

Genomic Analysis ◽

Resistance Breeding ◽

Genetic Distances ◽

Stripe Rust Resistance ◽

Comparative Genomic

ABSTRACT: Stripe rust, caused by Puccinia striiformis is one of the most destructive diseases of wheat worldwide. CH5389 is a wheat-Thinopyrum intermedium derived line conferring stripe rust resistance. Genetic analyses of seedlings of F2 populations and F2:3 families developed by crossing CH5389 and susceptible common wheat revealed that stripe rust resistance in CH5389 was controlled by a single dominant gene that was designated YrCH5389. Eight SSR and EST-PCR polymorphic markers on chromosome 3AL were identified in F2 population of CH5389/Taichung29. The YrCH5389 was flanked by EST marker BE405348 and SSR marker Xwmc388 on chromosome 3AL with genetic distances of 2.2 and 4.6 cM, respectively. Comparative genomic analysis demonstrated that the orthologous genomic region of YrCH5389 covered 990 kb in rice, 640 kb in Brachypodium, and 890 kb in sorghum. Based on the locations of the markers, the resistance gene was located to chromosome deletion bin 3AL-0.85-1.00. Because there are no officially named stripe rust resistance genes on the 3AL chromosome, the YrCH5389 should be designated as a new resistance gene. These linkage markers could be useful for marker-assisted selection in wheat resistance breeding.

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Genetics of stripe rust resistance in two barley (Hordeum vulgare L.) genotypes

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.79.2.16 ◽

2019 ◽

Vol 79 (02) ◽

Author(s):

Vishnu Kumar ◽

S. C. Bhardwaj ◽

A. S. Kharub ◽

G. P. Singh

Keyword(s):

Stripe Rust ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Dominant Gene ◽

Malting Quality ◽

Stripe Rust Resistance ◽

Yield Reduction ◽

Adult Plant ◽

Hordeum Vulgare L ◽

F2 Population

Stripe rust inflicted by the pathogen Puccinia striiformis Westend. f. sp. hordei Eriks can cause marked grain yield reduction and deterioration of the malting quality in barley. Two barley genotypes, DWRB137 (DWR28/DWRUB64) and DWRB143 (DWRB73/DWR83) were identified as resistant (R) for three stripe rust races viz., 57 (0S0), M (1S0) and Q (5S0) at seedling and adult plant stages. These genotypes were crossed with two stripe rust susceptible (S) barley cultivars, RD2035 and Lakhan to study mode of inheritance of stripe rust resistance. All the F1 plants showed resistance against stripe rust race, 57 (0S0) indicating that the resistance (R) is dominant. The F2 generations derived from the crosses namely, Lakhan/DWRB137, Lakhan/DWRB143, RD2035/DWRB137 and RD2035/DWRB143 were tested with inoculum of race, 57 (0S0) under artificially inoculated conditions. The observed frequency of segregants in each F2 generation fit well in a theoretical ratio of 3(R):1(S) (χ 2(T) less than 0.01) indicating that the resistance against the tested race is controlled by a single dominant gene in the genotypes, DWRB137 and DWRB143. To validate the F2 hypothesis, the F3 progenies were also tested under above described conditions and followed discrete segregation of 1 (R): 2 (Segregating): 1(S) ratio in all the four crosses. Test of allelism was also conducted to establish the identity of resistance gene(s) present in the resistant genotypes. The F2 population derived from DWRB137/DWRB143 (R × R) cross fit to 15(R):1(S) ratio showing that the two genotypes had different resistance genes.

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Genetic Analysis and Molecular Mapping of Wheat Genes Conferring Resistance to the Wheat Stripe Rust and Barley Stripe Rust Pathogens

Phytopathology ◽

10.1094/phyto-95-0427 ◽

2005 ◽

Vol 95 (4) ◽

pp. 427-432 ◽

Cited By ~ 29

Author(s):

Vihanga Pahalawatta ◽

Xianming Chen

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Molecular Mapping ◽

Puccinia Striiformis ◽

Dominant Gene ◽

Wheat Genotypes ◽

Barley Cultivars ◽

Barley Stripe Rust ◽

Rust Pathogens ◽

Barley Genotypes

Stripe rust is one of the most important diseases of wheat and barley worldwide. On wheat it is caused by Puccinia striiformis f. sp. tritici and on barley by P. striiformis f. sp. hordei Most wheat genotypes are resistant to P. striiformis f. sp. hordei and most barley genotypes are resistant to P. striiformis f. sp. tritici. To determine the genetics of resistance in wheat to P. striiformis f. sp. hordei, crosses were made between wheat genotypes Lemhi (resistant to P. striiformis f. sp. hordei) and PI 478214 (susceptible to P. striiformis f. sp. hordei). The greenhouse seedling test of 150 F2 progeny from the Lemhi × PI 478214 cross, inoculated with race PSH-14 of P. striiformis f. sp. hordei, indicated that Lemhi has a dominant resistance gene. The single dominant gene was confirmed by testing seedlings of the F1, BC1 to the two parents, and 150 F3 lines from the F2 plants with the same race. The tests of the F1, BC1, and F3 progeny with race PSH-48 of P. striiformis f. sp. hordei and PST-21 of P. striiformis f. sp. tritici also showed a dominant gene for resistance to these races. Cosegregation analyses of the F3 data from the tests with the two races of P. striiformis f. sp. hordei and one race of P. striiformis f. sp. tritici suggested that the same gene conferred the resistance to both races of P. striiformis f. sp. hordei, and this gene was different but closely linked to Yr21, a previously reported gene in Lemhi conferring resistance to race PST-21 of P. striiformis f. sp. tritici. A linkage group consisting of 11 resistance gene analog polymorphism (RGAP) markers was established for the genes. The gene was confirmed to be on chromosome 1B by amplification of a set of nullitetrasomic Chinese Spring lines with an RGAP marker linked in repulsion with the resistance allele. The genetic information obtained from this study is useful in understanding interactions between inappropriate hosts and pathogens. The gene identified in Lemhi for resistance to P. striiformis f. sp. hordei should provide resistance to barley stripe rust when introgressed into barley cultivars.

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