scholarly journals Virulence and Molecular Diversity in Colletotrichum lindemuthianum from South, Central, and North America

1997 ◽  
Vol 87 (12) ◽  
pp. 1184-1191 ◽  
Author(s):  
R. S. Balardin ◽  
A. M. Jarosz ◽  
J. D. Kelly
Keyword(s):  
United States ◽  
Molecular Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Area ◽  
The United States ◽  
Colletotrichum Lindemuthianum ◽  
Gene Pools ◽  
Molecular Polymorphism ◽  
South Central ◽  
Differential Cultivars

Isolates of Colletotrichum lindemuthianum (138 total) from Argentina, Brazil, the Dominican Republic, Honduras, Mexico, and the United States were characterized into 41 races based on virulence to 12 differential cultivars of Phaseolus vulgaris. These 41 races were categorized into two groups: those found over a wide geographic area and those restricted to a single country. Races 7, 65, and 73 were widespread. Race 73 was the most common (28%). Race 7 was found once in Argentina and Mexico but at a higher frequency in the United States. Race 65 was found repeatedly in Brazil and the United States. Although 39% of the races were detected repeatedly and three races were widespread, no race was isolated from both P. vulgaris gene pools. Phenetic analyses showed no obvious patterns correlated with virulence clusters. No geographic pattern was evident. Molecular polymorphism generated by random amplified polymorphic DNA confirmed the extensive variability in virulence of C. lindemuthianum. Virulence phenotypes were grouped into 15 clusters. The two largest clusters contained isolates from all the geographic regions sampled. Molecular polymorphism was observed among isolates from races 65 and 73 within and among countries, except among Bra-zilian isolates of race 65. The genetic diversity of C. lindemuthianum was greatest in Mexico and Honduras. Our data suggest that C. lindemuthianum may not be highly structured to specific Phaseolus gene pools.

scholarly journals Interaction between Colletotrichum lindemuthianum Races and Gene Pool Diversity in Phaseolus vulgaris

1998 ◽  
Vol 123 (6) ◽  
pp. 1038-1047 ◽  
Author(s):  
R.S. Balardin ◽  
J.D. Kelly
Keyword(s):  
United States ◽  
Principal Component Analysis ◽  
Phaseolus Vulgaris ◽  
Dominican Republic ◽  
Gene Pool ◽  
Principal Component ◽  
The United States ◽  
Colletotrichum Lindemuthianum ◽  
Gene Pools ◽  
Phenetic Analysis

Sixty-two genetically diverse modern and traditional Phaseolus vulgaris L. cultivars from Brazil, the Dominican Republic, Honduras, Mexico, the Netherlands, and the United States, representative of the Andean and Middle American gene pools, were selected to study the interaction with distinct races of Colletotrichum lindemuthianum (Sacc. & Magnus) Lams.-Scrib. Principal component and phenetic analyses were conducted on the disease reaction to inoculation with 34 races of C. lindemuthianum from Argentina, Brazil, Colombia, Costa Rica, the Dominican Republic, Honduras, Mexico, Peru, and the United States. The principal component analysis revealed four clusters in which only one cluster consisted of cultivars from both gene pools. Bean genotypes clustered based on the gene pool origin of the resistance genes present, regardless of the actual gene pool of the host genotype. Middle American genotypes in cluster A carried Andean resistance genes. Further grouping of genotypes based on overall level of resistance within each gene pool was observed. Clusters A and C consisted of the most resistant genotypes from both gene pools. The distribution of genotypes generated by the phenetic analysis, placed the most resistant and susceptible genotypes of the anthracnose differential series at the extremities of the phenogram, providing support for the range in genotypic resistance exhibited by members of the differential series. Races of C. lindemuthianum isolated from Middle American genotypes showed broad virulence on germplasm from both gene pools, whereas races with Andean reaction showed high virulence only on Andean germplasm. The reduced virulence of Andean races on Middle American genotypes suggests selection of virulence factors congruent with diversity in P. vulgaris. In addition, races of C. lindemuthianum formed two clusters corresponding to the Middle American and Andean reaction groups based on the phenetic analysis. In the principal component analysis, most races with the Andean reaction were observed in the clusters C and D, except races 15 and 23 which clustered with Middle American races in cluster B. Only races 38, 39 and 47 from the Dominican Republic showed high similarity in both multivariate analyses and clustered based on geographic origin. Races from other countries showed no geographic effect. The overlapping of specific races, however, with races from different reaction groups might indicate that this group of isolates possesses factors of virulence to both host gene pools. Data based on virulence supports variability in C. lindemuthianum structured with diversity in P. vulgaris.

scholarly journals Geographical Distribution, Pathotype Characterization, and Molecular Diversity of Colletotrichum lindemuthianum in Greece and Resistance of Greek Bean Cultivars

Plant Disease ◽  
2007 ◽  
Vol 91 (11) ◽  
pp. 1379-1385 ◽  
Author(s):  
G. A. Bardas ◽  
O. Koutita ◽  
K. Tzavella-Klonari
Keyword(s):  
Molecular Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Agricultural Practices ◽  
Rflp Analysis ◽  
Population Diversity ◽  
Internal Transcribed Spacers ◽  
Colletotrichum Lindemuthianum ◽  
Main Cluster ◽  
Differential Cultivars ◽  
First Time

Thirty-five Colletotrichum lindemuthianum isolates were obtained from bean (Phaseolus vulgaris) fields in 23 prefectures of Greece during a survey conducted in 2003 and 2004. Race characterization based on the standardized set of 12 differential cultivars demonstrated the presence of races 2, 6, and 22, which are reported for first time in Greece, while race 22 is reported for the first time in the world. In addition, pathotype diversity showed significant correlation with the geographical origin of these isolates. All three races caused disease symptoms only on cultivars of Andean origin, suggesting that Greek isolates originated from South American countries. Virulence spectrum of the same isolates was also examined on a set of 30 Greek bean cultivars showing seven types of virulence. Based on their reactions to the pathogen isolates, Greek cultivars were divided into nine groups. Among these cultivars, two (Ithomi FS60 and Larisa) were resistant to all tested isolates, including the reference isolates. Molecular diversity was detected using random amplified polymorphic DNA (RAPD) primers and the restriction fragment length polymorphism (RFLP) analysis of the ribosomal DNA internal transcribed spacers, which revealed two main clusters of isolates. Thirty-two out of 35 isolates belonged to the same main cluster in both methods, indicating that Greek isolates have genotypic similarities. This study provides the information on population diversity of C. lindemuthianum, which can be useful in agricultural practices and in plant breeding programs.

The Empire's New Frontiers

2003 ◽  
Vol 102 (667) ◽  
pp. 383-387 ◽  
Author(s):  
Michael T. Klare
Keyword(s):  
United States ◽  
Nineteenth Century ◽  
Great Britain ◽  
The United States ◽  
Late Nineteenth Century ◽  
Natural Behavior ◽  
Central Eurasia ◽  
South Central ◽  
Strategic Position ◽  
Late Nineteenth

The United States … wants to enhance its own strategic position in south-central Eurasia, much as Great Britain attempted in the late nineteenth century. This effort encompasses anti-terrorism and the pursuit of oil, but many in Washington also see it as an end in itself—as the natural behavior of a global superpower engaged in global dominance.

scholarly journals 692. Effects of Regional Climatic Variability on West Nile Virus Outbreaks in the United States

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S249-S250
Author(s):  
Raghesh Varot Kangath ◽  
Buddhika Maduraperuma ◽  
Juliana Souza Borges ◽  
Rajasreepai Ramachandrapai
Keyword(s):  
United States ◽  
High Temperature ◽  
Population Density ◽  
Climatic Variability ◽  
The United States ◽  
Climatic Data ◽  
Cca Ordination ◽  
Neuroinvasive Disease ◽  
South Central ◽  
Risk Areas

Abstract Background Transmission of WNV to humans in the United States typically occurs between June and September since warm temperatures accelerate mosquito life cycle. Precipitation can cause increase in aquatic breeding but outbreaks often depends upon human water management. We examine epidemiology, patterns of WNV disease transmission, and identification of high-risk areas in the United States from 2003 to 2014. Methods Trends and relationships of WNV cases and climatic factors were analyzed among the regions of the United States from 2003 to 2014. Human WNV tabulate data and climatic data were obtained from Centers for Disease Control, and NOAA and Climate Data Guide, respectively. Canonical correspondence analysis (CCA) was performed using variables: (i) neuroinvasive disease cases, non-neuroinvasive disease cases, deaths, presumptiveviremic blood donors, (ii) precipitation, temperature, Palmer Drought Severity Index (PDSI) and population density. The CCA ordination was explained the variability between WNV disease cases andclimatic variables. Biplots were used to visualize the associations between WNV cases and climatic anomalies. Results We compared the state wise WNV disease cases in relation to climatic and population density in the United States from 2003 to 2014. A total of 4,064 cases in 2006, 956 cases in 2010 and, 2,141 cases in 2014 were reported in the 32 states of the United States. Colorado state reported the highest WNV cases in 2003 (2,947 cases; 33%), followed by Texas in 2012 (1,868 cases; 35%) and California in 2014 (801 case; 37%). CCA ordination showed distinguishable clustering patterns between south central (Texas, Louisiana, Mississippi, Arkansas, and Oklahoma) and northern Great Plains (North Dakota, South Dakota, and Nebraska) regions (Figure 1). High temperature and prolong drought were the most important variable predictor for high WNV outbreak. Conclusion Vector control methods focusing on prevention must be implemented to avoid epidemics of WNV if high temperature is leading to an unusual drought especially at the risk areas, such as Texas and California. However, high temperature with moist spell anomalies in the south central region showed a negative influence on WNV outbreak. Disclosures All authors: No reported disclosures.

scholarly journals Performance of Landscape Roses Grown with Minimal Input in the North-central, Central, and South-central United States

2017 ◽  
Vol 27 (5) ◽  
pp. 718-730 ◽  
Author(s):  
David C. Zlesak ◽  
Randy Nelson ◽  
Derald Harp ◽  
Barbara Villarreal ◽  
Nick Howell ◽  
...  
Keyword(s):  
United States ◽  
National Level ◽  
The United States ◽  
The South ◽  
Flower Number ◽  
North Central ◽  
The North ◽  
South Central ◽  
Central United States ◽  
Plant Hardiness

Landscape roses (Rosa sp.) are popular flowering shrubs. Consumers are less willing or able to maintain landscape beds than in years past and require plants that are not only attractive, but well-adapted to regional climatic conditions, soil types, and disease and pest pressures. Marketing and distribution of rose cultivars occurs on a national level; therefore, it is difficult for U.S. consumers in the U.S. Department of Agriculture (USDA) Plant Hardiness Zones 3 to 5 to identify well-adapted, cold-hardy cultivars. Identifying suitable cultivars that have strong genetic resistance to pests and disease and that will tolerate temperature extremes without winter protection in the USDA Plant Hardiness Zones 3 to 5 is of tremendous value to consumers and retailers in northern states. Twenty landscape rose cultivars, primarily developed in north-central North America, were evaluated at five locations in the United States (three in the north-central United States, one in the central United States, and one in the south-central United States) using the low-input, multiyear Earth-Kind® methodology. Six roses had ≥75% plant survival at the end of the study and were in the top 50% of performers for overall mean horticultural rating at each of the three north-central U.S. sites: ‘Lena’, ‘Frontenac’, ‘Ole’, ‘Polar Joy’, ‘Sunrise Sunset’, and ‘Sven’. Five of these six roses met the same criteria at the central United States (exception ‘Lena’) and the south-central United States (exception ‘Polar Joy’) sites. Cultivar, rating time, and their interaction were highly significant, and block effects were not significant for horticultural rating for all single-site analyses of variance. Significant positive correlations were found between sites for flower number, flower diameter, and overall horticultural rating. Significant negative correlations were found between flower number and diameter within each site and also between black spot (Diplocarpon rosae) lesion size from a previous study and overall horticultural rating for three of the five sites. Cane survival ratings were not significantly correlated with overall horticultural rating, suggesting some cultivars can experience severe winter cane dieback, yet recover and perform well. Data from this study benefit multiple stakeholders, including nurseries, landscapers, and consumers, with evidence-based regional cultivar recommendations and breeders desiring to identify regionally adapted parents.

scholarly journals Dissecting the Financial Relationship between Industry and Academic Neurosurgery

Neurosurgery ◽  
2020 ◽  
Author(s):  
Mustafa Motiwala ◽  
Michael J Herr ◽  
Sripraharsha S Jampana Raju ◽  
Jock Lillard ◽  
Sonia Ajmera ◽  
...  
Keyword(s):  
United States ◽  
New England ◽  
Geographic Location ◽  
Bimodal Distribution ◽  
The United States ◽  
Median Number ◽  
Career Stage ◽  
Entire Cohort ◽  
South Central ◽  
And Gender

Abstract BACKGROUND Established by the Centers for Medicare and Medicaid Services (CMS), the Open Payments Database (OPD) has reported industry payments to physicians since August 2013. OBJECTIVE To evaluate the frequency, type, and value of payments received by academic neurosurgeons in the United States over a 5-yr period (2014-2018). METHODS The OPD was queried for attending neurosurgeons from all neurosurgical training programs in the United States (n = 116). Information from the OPD was analyzed for the entire cohort as well as for comparative subgroup analyses, such as career stage, subspecialty, and geographic location. RESULTS Of all identified neurosurgeons, 1509 (95.0%) received some payment from industry between 2014 and 2018 for a total of 106 171 payments totaling $266 407 458.33. A bimodal distribution was observed for payment number and total value: 0 to 9 (n = 438) vs > 50 (n = 563) and 0-$1000 (n = 418) vs >$10 000 (n = 653), respectively. Royalty/License was the most common type of payment overall (59.6%; $158 723 550.57). The median number (40) and value ($8958.95) of payments were highest for mid-career surgeons. The South-Central region received the most money ($117 970 036.39) while New England received the greatest number of payments (29 423). Spine surgeons had the greatest median number (60) and dollar value ($20 551.27) of payments, while pediatric neurosurgeons received the least (8; $1108.29). Male neurosurgeons received a greater number (31) and value ($6395.80) of payments than their female counterparts (11, $1643.72). CONCLUSION From 2014 to 2018, payments to academic neurosurgeons have increased in number and value. Dollars received were dependent on geography, career stage, subspecialty and gender.

Knockdown Resistance-Associated Mutations Dominate Populations of the Common Bed Bug (Hemiptera: Cimicidae) Across the South Central United States

2019 ◽  
Vol 56 (6) ◽  
pp. 1678-1683 ◽  
Author(s):  
Jeffrey G Holleman ◽  
Grant A Robison ◽  
Ian J Bellovich ◽  
Warren Booth
Keyword(s):  
United States ◽  
Insecticide Resistance ◽  
The United States ◽  
Knockdown Resistance ◽  
The South ◽  
Α Subunit ◽  
Old World ◽  
Bed Bug ◽  
South Central ◽  
Central United States

Abstract Despite awareness of the mutations conferring insecticide resistance in the bed bug, Cimex lectularius L. (Hemiptera: Cimicidae), within the United States few studies address the distribution and frequency of these. Within the United States, studies have focused on collections made along the East Coast and Midwest, documenting the occurrence of two mutations (V419L and L925I) within the voltage-gated sodium channel α-subunit gene shown to be associated with knockdown resistance (kdr) to pyrethroids. Here, the distribution and frequency of the V419L and L925I site variants is reported from infestations sampled within Oklahoma and its immediately adjacent states. Additionally, the presence of a mutation previously undocumented in the United States (I935F) is noted. While novel in the United States, this mutation has previously been reported in Australian and Old World populations. No infestations were found to harbor wild-type individuals, and hence susceptible, at each of the three sites. Instead, ~21% were found to possess the resistant mutation at the L925I site (haplotype B), ~77% had mutations at both the V419L and L925I sites (haplotype C), and 2% possessed the mutation at the L936F site (haplotype Ab). The high frequency of haplotype C corresponds to previous studies in the United States, and contrasts dramatically with those of the Old World and Australia. The data presented here provide insight into the contemporary occurrence of kdr-associated insecticide resistance in the South Central United States, a region for which data have previously been absent. These data suggest that New World and Old World/Australian infestations are likely to have originated from different origins.

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