scholarly journals The Novel Pig In Vitro Maturation System to Improve Developmental Competence of Oocytes Derived from Atretic Nonvascularized Follicles

2016 ◽  
Vol 95 (4) ◽  
pp. 76-76 ◽  
Author(s):  
A. Okamoto ◽  
M. Ikeda ◽  
A. Kaneko ◽  
C. Kishida ◽  
M. Shimada ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Developmental Competence ◽  
The Novel

scholarly journals Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilization and developmental competence of porcine oocytes

2017 ◽  
Vol 52 (6) ◽  
pp. 969-975 ◽  
Author(s):  
T‐V Nguyen ◽  
F Tanihara ◽  
LTK Do ◽  
Y Sato ◽  
M Taniguchi ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
In Vitro Maturation ◽  
Developmental Competence ◽  
Acid Supplementation

scholarly journals Impact of L-carnitine supplementation on the in vitro developmental competence and cryotolerance of buffalo embryos

2021 ◽  
pp. 3164-3169
Author(s):  
Mohamed M. M. El-Sokary ◽  
Al-Shimaa Al-H. H. El-Naby ◽  
Amal R. Abd El Hameed ◽  
Karima Gh. M. Mahmoud ◽  
T. H. Scholkamy
Keyword(s):  
Embryo Culture ◽  
In Vitro Maturation ◽  
Culture Media ◽  
Effective Concentration ◽  
Developmental Competence ◽  
Carnitine Supplementation ◽  
Oocyte Developmental Competence ◽  
High Lipid ◽  
Significant Difference

Background and Aim: Despite many trials, buffalo embryos have poor cryosurvivability because of their high lipid content. L-carnitine was found to be a lipid-reducing agent when added to oocyte and embryo culture media. The study aimed to determine the most effective concentration of L-carnitine to improve the oocyte developmental competence and cryotolerance of buffalo embryos. Materials and Methods: In vitro maturation and embryo culture media were supplemented with four concentrations of L-carnitine: 0 (control), 0.25, 0.5, and 1 mM. Good-quality embryos on 7 days were vitrified using mixtures of dimethyl sulfoxide and ethylene glycol at two concentrations (3.5 and 7 M). Results: The result showed that the cleavage and morula rates were significantly (p<0.05) higher in the 0.5 mM group. Blastocyst rates were significantly (p<0.05) higher at both 0.5 and 1 mM. The rates of viable embryos directly after thawing were significantly (p<0.05) increased in the 0.5 mM group. No significant difference was found in embryos cultured for 24 h after warming among all the groups. Conclusion: The addition of L-carnitine at a concentration of 0.5 mM to the culture media improves the oocyte developmental competence and cryotolerance of buffalo embryos directly after warming but not after 24 h of culture. Nevertheless, further studies must identify how L-carnitine exerts its beneficial micromechanisms.

scholarly journals Milrinone Treatment of Bovine Oocytes During In Vitro Maturation Benefits Production of Nuclear Transfer Embryos by Improving Enucleation Rate and Developmental Competence

2012 ◽  
Vol 58 (4) ◽  
pp. 476-483 ◽  
Author(s):  
Kenji NARUSE ◽  
Kosuke IGA ◽  
Manabu SHIMIZU ◽  
Naoki TAKENOUCHI ◽  
Satoshi AKAGI ◽  
...  
Keyword(s):  
Nuclear Transfer ◽  
In Vitro Maturation ◽  
Developmental Competence ◽  
Bovine Oocytes

Iloprost affects in vitro maturation and developmental competence of bovine oocytes

2020 ◽  
Vol 157 ◽  
pp. 286-296
Author(s):  
Ilona Kowalczyk-Zieba ◽  
Dorota Boruszewska ◽  
Katarzyna Suwik ◽  
Joanna Staszkiewicz-Chodor ◽  
Joanna Jaworska ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Developmental Competence ◽  
Bovine Oocytes

scholarly journals α-Tocopherol modifies the expression of genes related to oxidative stress and apoptosis during in vitro maturation and enhances the developmental competence of rabbit oocytes

2018 ◽  
Vol 30 (12) ◽  
pp. 1728 ◽  
Author(s):  
M. Arias-Álvarez ◽  
R. M. García-García ◽  
J. López-Tello ◽  
P. G. Rebollar ◽  
A. Gutiérrez-Adán ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
In Vitro Maturation ◽  
Cumulus Cell ◽  
Rabbit Model ◽  
Developmental Competence ◽  
Antioxidant Agents ◽  
Junction Protein

The developmental competence of in vitro maturation (IVM) oocytes can be enhanced by antioxidant agents. The present study investigated, for the first time in the rabbit model, the effect of adding α-tocopherol (0, 100, 200 and 400 µM) during IVM on putative transcripts involved in antioxidant defence (superoxide dismutase 2, mitochondrial (SOD2), glutathione peroxidase 1 (GPX1), catalase (CAT)), cell cycle regulation and apoptosis cascade (apoptosis tumour protein 53 (TP53), caspase 3, apoptosis-related cysteine protease (CASP3)), cell cycle progression (cellular cycle V-Akt murine thymoma viral oncogene homologue 1 (AKT1)), cumulus expansion (gap junction protein, alpha 1, 43 kDa (GJA1) and prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclo-oxygenase) (PTGS2)) and metabolism (glucose-6-phosphate dehydrogenase (G6PD)). Meiotic progression, mitochondrial reallocation, cumulus cell apoptosis and the developmental competence of oocytes after IVF were also assessed. Expression of SOD2, CAT, TP53, CASP3 and GJA1 was downregulated in cumulus–oocyte complexes (COCs) after IVM with 100 μM α-tocopherol compared with the group without the antioxidant. The apoptotic rate and the percentage of a non-migrated mitochondrial pattern were lower in COCs cultured with 100 μM α-tocopherol, consistent with better-quality oocytes. In fact, early embryo development was improved when 100 μM α-tocopherol was included in the IVM medium, but remained low compared with in vivo-matured oocytes. In conclusion, the addition of 100 μM α-tocopherol to the maturation medium is a suitable approach to manage oxidative stress and apoptosis, as well as for increasing the in vitro developmental competence of rabbit oocytes.

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