Anti‐tumor activity of the ethanolic extract of
            Micromeria fruticosa
            on human breast and colon cancer cells

(1) Background: Calcium electroporation (CaEP) is based on the application of electrical pulses to permeabilize cells (electroporation) and allow cytotoxic doses of calcium to enter the cell. (2) Methods: In this work, we have used doxorubicin-resistant (DX) and non-resistant models of human breast cancer (MCF-7/DX, MCF-7/WT) and colon cancer cells (LoVo, LoVo/DX), and investigated the susceptibility of the cells to extracellular Ca2+ and electric fields in the 20 ns–900 ns pulse duration range. (3) Results: We have observed that colon cancer cells were less susceptible to PEF than breast cancer cells. An extracellular Ca2+ (2 mM) with PEF was more disruptive for DX-resistant cells. The expression of glycoprotein P (MDR1, P-gp) as a drug resistance marker was detected by the immunofluorescent (CLSM) method and rhodamine-123 efflux as an MDR1 activity. MDR1 expression was not significantly modified by nanosecond electroporation in multidrug-resistant cells, but a combination with calcium ions significantly inhibited MDR1 activity and cell viability. (4) Conclusions: We believe that PEF with calcium ions can reduce drug resistance by inhibiting drug efflux activity. This phenomenon of MDR mechanism disruption seems promising in anticancer protocols.

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Cytotoxic Activity and Apoptosis Induction of Ethanolic Extract of Pericarps of Mangosteen (Garcinia mangostana Linn.) on WiDr Cells and Interaction Study of Alpha-mangosteen to IKK and VEGF Based on Molecular Docking

Indonesian Journal of Cancer Chemoprevention ◽

10.14499/indonesianjcanchemoprev4iss1pp470-476 ◽

2013 ◽

Vol 4 (1) ◽

pp. 470

Author(s):

Annishfia Lailatur Rohmah ◽

Fikri Amalia ◽

Erlina Rivanti ◽

Dyaningtyas Dewi Pamungkas Putri ◽

Nunuk Aries Nurulita

Keyword(s):

Colon Cancer ◽

Molecular Docking ◽

Cancer Cells ◽

Cytotoxic Effect ◽

Ethanolic Extract ◽

Apoptosis Induction ◽

Colon Cancer Cells ◽

Garcinia Mangostana ◽

Double Staining Method ◽

Native Ligand

One of the compounds found efficacious as an anti-proliferative on colon cancer is alpha-mangosteen, a xanthon compound that is abundant in pericarp of mangosteen. This study focused to evaluate anticancer activity of the ethanolic extract of pericarp of mangosteen (Garcinia mangostana Linn.) on WiDr colon cancer cells and to observe the affinity of alpha-mangosteen in inhibiting IKK and VEGF. Cytotoxic effect was tested by MTT assay and apoptosis induction was evaluated by double staining method on WiDr colon cancer cells, while interaction between alpha-mangosteen to the receptors was measured by molecular docking. The ethanolic extract was proven to have cytotoxic activity against WiDr colon cancer cells with IC50 of 25 µg/mL. In addition, the observation of apoptosis induction by double-staining method showed that apoptosis associated the cytotoxic effect of ethanolic extract of pericarp of mangosteen (EPM) on WiDr colon cancer cells. Molecular docking showed that active compounds in pericarp of mangosteen could compete with the native ligand of VEGF because the docking score of alpha-mangosteen was almost equal with native ligand. From these results we could be concluded that the cytotoxic effect of EPM to WiDr cells was mediated by cell apoptosis. This extract was potential to be developed as chemopreventive agent.Keywords : Garcinia mangostana Linn., cytotoxic effect, apoptosis, WiDr cell, molecular docking

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Ethanolic Extract of Citrus reticulata Peel Inhibits the Migration of WiDr Colon Cancer Cells

Indonesian Journal of Cancer Chemoprevention ◽

10.14499/indonesianjcanchemoprev11iss2pp60-66 ◽

2020 ◽

Vol 11 (2) ◽

pp. 60

Author(s):

Yoni Astuti ◽

Aulia Primasari

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Migration And Invasion ◽

Citrus Reticulata ◽

Colon Cancer Cells ◽

Migration Assay ◽

Widr Cell ◽

Cancer Line

Colorectal cancer is third rank on the cancer cases in Indonesia. To cure the cancer needs big cost and lot of effort. On the other side, the side effect of medicine or chemotherapy on patient need to reduce. Cancer cell spread to other tissue based on its migration and invasion ability. Citrus reticulata peel contains flavonoid such as Tangeretin and Nobiletin, both of this compounds have anticancer activity. The aims of this study is to reveals the potency of ethanol extract of Citrus reticulata peel on the inhibition of migration on WiDr colon cancer cells. The toxicity of ethanol extract of Citurs reticulata peel on WiDr colon cancer line was measured using 3-(4,5-dimethyltiazol-2-il)-2,5-diphenyltrazolium bromide (MTT) assay and investigate the cell migration was using scratch wound healing assay. The ethanol extract of Citrus reticulata peel showed the value of inhibitory concentration 50 (IC50) was 184.5 μg/mL, this result categorize as moderate cytotoxic. Meanwhile the migration assay showed that the deceleration of migration occurred on 0.5 IC50, 0.33 IC50 and 0.25 IC50 during 24 h and 36 h incubation, event thought there were not significant different (p>0.05). The ethanol extract of Citrus reticulata peel has a potential migration inhibition on WiDr cell line.Keywords: Citrus reticulata, WiDr cell line, migration

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The phytoestrogen Genistein inhibits the anti-tumor activity of cisplatin in MCF-7 breast and HT29 colon cancer cells

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-0033-1336640 ◽

2013 ◽

Vol 121 (03) ◽

Cited By ~ 1

Author(s):

H Xiao Juan ◽

M Xie ◽

P Diel

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Colon Cancer Cells ◽

Anti Tumor Activity ◽

Mcf 7

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