Anticarcinogenic Effects of the Ethanolic Extract ofSalix aegyptiacain Colon Cancer Cells: Involvement of Akt/PKB and MAPK Pathways

2013 ◽  
Vol 65 (7) ◽  
pp. 1045-1058 ◽  
Author(s):  
Shabnam Enayat ◽  
Müşerref Şeyma Ceyhan ◽  
Arif Ahmet Başaran ◽  
Mayda Gürsel ◽  
Sreeparna Banerjee
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Ethanolic Extract ◽  
Colon Cancer Cells ◽  
Mapk Pathways

scholarly journals Cytotoxic Activity and Apoptosis Induction of Ethanolic Extract of Pericarps of Mangosteen (Garcinia mangostana Linn.) on WiDr Cells and Interaction Study of Alpha-mangosteen to IKK and VEGF Based on Molecular Docking

2013 ◽  
Vol 4 (1) ◽  
pp. 470
Author(s):  
Annishfia Lailatur Rohmah ◽  
Fikri Amalia ◽  
Erlina Rivanti ◽  
Dyaningtyas Dewi Pamungkas Putri ◽  
Nunuk Aries Nurulita
Keyword(s):  
Colon Cancer ◽  
Molecular Docking ◽  
Cancer Cells ◽  
Cytotoxic Effect ◽  
Ethanolic Extract ◽  
Apoptosis Induction ◽  
Colon Cancer Cells ◽  
Garcinia Mangostana ◽  
Double Staining Method ◽  
Native Ligand

One of the compounds found efficacious as an anti-proliferative on colon cancer is alpha-mangosteen, a xanthon compound that is abundant in pericarp of mangosteen. This study focused to evaluate anticancer activity of the ethanolic extract of pericarp of mangosteen (Garcinia mangostana Linn.) on WiDr colon cancer cells and to observe the affinity of alpha-mangosteen in inhibiting IKK and VEGF. Cytotoxic effect was tested by MTT assay and apoptosis induction was evaluated by double staining method on WiDr colon cancer cells, while interaction between alpha-mangosteen to the receptors was measured by molecular docking. The ethanolic extract was proven to have cytotoxic activity against WiDr colon cancer cells with IC50 of 25 µg/mL. In addition, the observation of apoptosis induction by double-staining method showed that apoptosis associated the cytotoxic effect of ethanolic extract of pericarp of mangosteen (EPM) on WiDr colon cancer cells. Molecular docking showed that active compounds in pericarp of mangosteen could compete with the native ligand of VEGF because the docking score of alpha-mangosteen was almost equal with native ligand. From these results we could be concluded that the cytotoxic effect of EPM to WiDr cells was mediated by cell apoptosis. This extract was potential to be developed as chemopreventive agent.Keywords : Garcinia mangostana Linn., cytotoxic effect, apoptosis, WiDr cell, molecular docking 

scholarly journals Ethanolic Extract of Citrus reticulata Peel Inhibits the Migration of WiDr Colon Cancer Cells

2020 ◽  
Vol 11 (2) ◽  
pp. 60
Author(s):  
Yoni Astuti ◽  
Aulia Primasari
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Migration And Invasion ◽  
Citrus Reticulata ◽  
Colon Cancer Cells ◽  
Migration Assay ◽  
Widr Cell ◽  
Cancer Line

Colorectal cancer is third rank on the cancer cases in Indonesia. To cure the cancer needs big cost and lot of effort. On the other side, the side effect of medicine or chemotherapy on patient need to reduce. Cancer cell spread to other tissue based on its migration and invasion ability. Citrus reticulata peel contains flavonoid such as Tangeretin and Nobiletin, both of this compounds have anticancer activity. The aims of this study is to reveals the potency of ethanol extract of Citrus reticulata peel on the inhibition of migration on WiDr colon cancer cells. The toxicity of ethanol extract of Citurs reticulata peel on WiDr colon cancer line was measured using 3-(4,5-dimethyltiazol-2-il)-2,5-diphenyltrazolium bromide (MTT) assay and investigate the cell migration was using scratch wound healing assay. The ethanol extract of Citrus reticulata peel showed the value of inhibitory concentration 50 (IC50) was 184.5 μg/mL, this result categorize as moderate cytotoxic. Meanwhile the migration assay showed that the deceleration of migration occurred on 0.5 IC50, 0.33 IC50 and 0.25 IC50 during 24 h and 36 h incubation, event thought there were not significant different (p>0.05). The ethanol extract of Citrus reticulata peel has a potential migration inhibition on WiDr cell line.Keywords: Citrus reticulata, WiDr cell line, migration

Anti‐tumor activity of the ethanolic extract of Micromeria fruticosa on human breast and colon cancer cells

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1 ◽  
Author(s):  
Eman Abu-Gharbieh ◽  
Waseem El-Huneidi ◽  
Naglaa G. Shehab ◽  
Khuloud Bajbouj ◽  
Arya Vinod ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Ethanolic Extract ◽  
Colon Cancer Cells ◽  
Human Breast ◽  
Anti Tumor Activity ◽  
Micromeria Fruticosa

scholarly journals Antioxidant and Chemoprevention Activity of Camelia Sinensis-Annona muricata Extract Combination against WiDr Cells Line

2021 ◽  
Vol 21 (2) ◽  
pp. 130-137
Author(s):  
Nazariah Putri ◽  
Iwan Dewanto ◽  
Rifki Febriansah
Keyword(s):  
Colon Cancer ◽  
Molecular Docking ◽  
Cancer Cells ◽  
Epigallocatechin Gallate ◽  
Ethanolic Extract ◽  
Assay Method ◽  
Antioxidant Compounds ◽  
Annona Muricata ◽  
Colon Cancer Cells ◽  
Ic50 Value

Antioxidant compounds have an essential role in inhibiting the process of cell proliferation and have a chemopreventive effect. This study aims to trace the presence of antioxidant compounds allegedly contained in tea leaves (Camelia Sinensis L.) and soursop leaves (Anonna muricata L.) and investigate their potency as chemopreventive agents. Research steps include (1) identify the active compounds using thin-layer chromatography (TLC); (2) find out the potential compounds against cancer cells by molecular docking using Autodock Vina; (3) conduct a potential antioxidant test using free radicals DPPH (1,1-diphenyl-2-pikrihidrazil); and (4) identify the cytotoxic effect on WiDr colon cancer cells test using MTT Assay method. The results showed that the ethanolic extract of Camellia sinensis-Annona muricata leaf combination was suspected of containing flavonoid compounds with Rf values of 0.66 and 0.68. Besides, the DPPH antioxidant test showed an IC50 value of 26.9 μg/mL. Cytotoxic potential against WiDr cells resulted in an IC50 value of 41 μg/mL. Furthermore, the molecular docking test of epigallocatechin gallate (EGCG) and Acetogenin compounds against bcl-xl target proteins showed the docking score of -8.1 kcal/mol and -6.7 kcal/mol, respectively. It concluded that the extract combination of Camelia Sinensis-Annona muricata leaf had strong potency as an antioxidant and chemopreventive agent against the WiDr colon cancer cells line.

scholarly journals The Cytotoxic Activity of Solanum Nigrum Ethanolic Extract on Widr Human Colon Cancer Cells

2011 ◽  
Vol 2 (3) ◽  
pp. 291
Author(s):  
Astrid Ayu Maruti ◽  
Ilham Augusta F. ◽  
Dyaningtyas Dewi Pamungkas Putri ◽  
Adam Hermawan ◽  
Muthi' Ikawati
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Mtt Assay ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Human Colon ◽  
Solanum Nigrum ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells

Solanum nigrum L. or Leunca in Indonesia has been traditionally used as a herbal plant, which is believed to have anti-tumor properties, although the mechanism for the activity remains unknown. The resecarch aim to examine the cytotoxic effect of the ethanolic extract of Solanum nigrum on WiDr human colon cancer cells. In this study, we prepared an ethanol extract from herb of Solanum nigrum and investigated the mechanism involved in its growth-inhibitory effect on WiDr human colon cancer cells. Herbs of Solanum nigrum dry powder is extracted with 70% ethanol then added into the WiDr cell culture in 96 wells plate in various concentration : 50, 100, 250, and 500 µg/ml. Cytotoxicity of the Solanum nigrum ethanolic extract  was analyzed with MTT assay on WiDr human colon cancer cell lines. Results from the MTT assay showed WiDr cells was weakly suppressed in the presence of the extract. The result of the assay also showed a very close correlation between the Solanum nigrum extract concentration and the surviving cell numbers which means the extract caused cell death in a dose-dependent fashion in WiDr cancer cells with the IC50 of 359,23 µg/ml. Collectively, the research suggest further studies to explore other chemopreventive possibilites of Solanum nigrum ethanolic extract.Keywords : colon cancer, MTT assay, cytotoxic, WiDr, Solanum nigrum

scholarly journals Activation of Akt and MAPK pathways enhances the tumorigenicity of CD133+ primary colon cancer cells

2010 ◽  
Vol 31 (8) ◽  
pp. 1376-1380 ◽  
Author(s):  
Y. K. Wang ◽  
Y. L. Zhu ◽  
F. M. Qiu ◽  
T. Zhang ◽  
Z. G. Chen ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Mapk Pathways ◽  
Primary Colon Cancer ◽  
Primary Colon

Leptin is a growth factor for human colon cancer cells

2001 ◽  
Vol 120 (5) ◽  
pp. A493-A493
Author(s):  
J HARDWICK ◽  
G VANDENBRINK ◽  
S VANDEVENTER ◽  
M PEPPELENBOSCH
Keyword(s):  
Colon Cancer ◽  
Growth Factor ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells
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