scholarly journals Cytotoxic Activity and Apoptosis Induction of Ethanolic Extract of Pericarps of Mangosteen (Garcinia mangostana Linn.) on WiDr Cells and Interaction Study of Alpha-mangosteen to IKK and VEGF Based on Molecular Docking

2013 ◽  
Vol 4 (1) ◽  
pp. 470
Author(s):  
Annishfia Lailatur Rohmah ◽  
Fikri Amalia ◽  
Erlina Rivanti ◽  
Dyaningtyas Dewi Pamungkas Putri ◽  
Nunuk Aries Nurulita
Keyword(s):  
Colon Cancer ◽  
Molecular Docking ◽  
Cancer Cells ◽  
Cytotoxic Effect ◽  
Ethanolic Extract ◽  
Apoptosis Induction ◽  
Colon Cancer Cells ◽  
Garcinia Mangostana ◽  
Double Staining Method ◽  
Native Ligand

One of the compounds found efficacious as an anti-proliferative on colon cancer is alpha-mangosteen, a xanthon compound that is abundant in pericarp of mangosteen. This study focused to evaluate anticancer activity of the ethanolic extract of pericarp of mangosteen (Garcinia mangostana Linn.) on WiDr colon cancer cells and to observe the affinity of alpha-mangosteen in inhibiting IKK and VEGF. Cytotoxic effect was tested by MTT assay and apoptosis induction was evaluated by double staining method on WiDr colon cancer cells, while interaction between alpha-mangosteen to the receptors was measured by molecular docking. The ethanolic extract was proven to have cytotoxic activity against WiDr colon cancer cells with IC50 of 25 µg/mL. In addition, the observation of apoptosis induction by double-staining method showed that apoptosis associated the cytotoxic effect of ethanolic extract of pericarp of mangosteen (EPM) on WiDr colon cancer cells. Molecular docking showed that active compounds in pericarp of mangosteen could compete with the native ligand of VEGF because the docking score of alpha-mangosteen was almost equal with native ligand. From these results we could be concluded that the cytotoxic effect of EPM to WiDr cells was mediated by cell apoptosis. This extract was potential to be developed as chemopreventive agent.Keywords : Garcinia mangostana Linn., cytotoxic effect, apoptosis, WiDr cell, molecular docking 

scholarly journals Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells

2012 ◽  
Vol 3 (2) ◽  
pp. 404 ◽  
Author(s):  
Yurista Gilang ◽  
Adam Hermawan ◽  
Aditya Fitriasari ◽  
Riris Istighfari Jenie
Keyword(s):  
Colon Cancer ◽  
Cytotoxic Effect ◽  
Staining Method ◽  
Apoptosis Induction ◽  
Double Staining ◽  
Colon Cancer Cells ◽  
Cytotoxic Effects ◽  
Chemotherapy Agent ◽  
Ic50 Value ◽  
Double Staining Method

Therapy of colon cancer by using 5-FU often causes problems of resistance. This encourages the development of co-chemotherapy agent. One of the compounds that could potentially be used as a co-chemotherapy agent is hesperidin. This study was conducted to determine the cytotoxic effects of hesperidin, 5-FU and the combination of them, as well as apoptosis induction in colon cancer cells WiDr. Cytotoxic effect of hesperidin, 5-FU, and its combination were observed using MTT assay. Observation of apoptosis was done by double staining method using ethidium bromide-acridin orange. Until 48 hours incubation, hesperidin showed no cytotoxic effects. Cytotoxic effects of 5-FU was observed after 48 hours with the IC50 value of 422 µM. However, hesperidin improved the cytotoxic effects of 5-FU at 48 hour incubation. Either single treatment of hesperidin 200µM or 5-FU 1500 µM did not trigger apoptosis, but combination of them led to the emergence of signs of apoptosis. Based on this study,it can be concluded thathesperidin is potential to be developed as a co-chemotherapy agent of 5-FU on colon cancer but still need further study on its molecular mechanisms.Keywords : hesperidin, 5-fluorouracil, WiDr cells, cytotoxic, apoptosis

scholarly journals Antioxidant and Chemoprevention Activity of Camelia Sinensis-Annona muricata Extract Combination against WiDr Cells Line

2021 ◽  
Vol 21 (2) ◽  
pp. 130-137
Author(s):  
Nazariah Putri ◽  
Iwan Dewanto ◽  
Rifki Febriansah
Keyword(s):  
Colon Cancer ◽  
Molecular Docking ◽  
Cancer Cells ◽  
Epigallocatechin Gallate ◽  
Ethanolic Extract ◽  
Assay Method ◽  
Antioxidant Compounds ◽  
Annona Muricata ◽  
Colon Cancer Cells ◽  
Ic50 Value

Antioxidant compounds have an essential role in inhibiting the process of cell proliferation and have a chemopreventive effect. This study aims to trace the presence of antioxidant compounds allegedly contained in tea leaves (Camelia Sinensis L.) and soursop leaves (Anonna muricata L.) and investigate their potency as chemopreventive agents. Research steps include (1) identify the active compounds using thin-layer chromatography (TLC); (2) find out the potential compounds against cancer cells by molecular docking using Autodock Vina; (3) conduct a potential antioxidant test using free radicals DPPH (1,1-diphenyl-2-pikrihidrazil); and (4) identify the cytotoxic effect on WiDr colon cancer cells test using MTT Assay method. The results showed that the ethanolic extract of Camellia sinensis-Annona muricata leaf combination was suspected of containing flavonoid compounds with Rf values of 0.66 and 0.68. Besides, the DPPH antioxidant test showed an IC50 value of 26.9 μg/mL. Cytotoxic potential against WiDr cells resulted in an IC50 value of 41 μg/mL. Furthermore, the molecular docking test of epigallocatechin gallate (EGCG) and Acetogenin compounds against bcl-xl target proteins showed the docking score of -8.1 kcal/mol and -6.7 kcal/mol, respectively. It concluded that the extract combination of Camelia Sinensis-Annona muricata leaf had strong potency as an antioxidant and chemopreventive agent against the WiDr colon cancer cells line.

Apoptotic and cytotoxic effect of bioactive fractions of Rhus trilobata on colon cancer cells CaCo-2

2016 ◽  
Vol 259 ◽  
pp. S193-S194
Author(s):  
L.E. Híjar-Soto ◽  
C. González-Horta ◽  
D. Chávez-Flores ◽  
B. Sánchez-Ramírez
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Cytotoxic Effect ◽  
Colon Cancer Cells

scholarly journals COMBINATION OF HEDYOTIS CORYMBOSA L. AND TINOSPORA CRISPA ETHANOLIC EXTRACT INCREASE CISPLATIN CYTOTOXICITY ON T47D BREAST CANCER CELLS

2018 ◽  
Vol 11 (7) ◽  
pp. 171 ◽  
Author(s):  
Rollando Rollando
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cytotoxic Effect ◽  
Ethanolic Extract ◽  
T47d Cell ◽  
Apoptosis Induction ◽  
Cell Accumulation ◽  
Cell Cycle Modulation

Objective: Ursolic acid was a compound found in Hedyotis corymbosa L., (HCoL) while berberine found in Tinospora crispa (TCa) which are proven to have cytotoxic effect to cancer cells. This research aims to review the effect of cisplatin, ethanolic extract of HCoL and TCa to the sensitivity increase on breast cancer cells, which will be confirmed through apoptosis induction and cell cycle modulation.Methods: The cytotoxic effect was tested using 3-(4,5-dimethylthiazol-2-il)-2,5-diphenyltetrazolium bromide assay on T47D cell using the IC50 parameter. The combination was tested by determining their combination index (CI) and cell viability. The combination effect of apoptosis induction and cell cycle modulation was observed using flow cytometry method.Results: The cytotoxic test result of the combination shows CI value of below 1 at the concentration of HCoL ethanolic extract as much as 1 μg/mL, TCa ethanolic extract as much as 6 μg/mL, and cisplatin as much as 2,5 μM. The combination of HCoL ethanolic extract, TCa ethanolic extract, and cisplatin results in phase S cell accumulation (29.98%) on breast cancer cell T47D and was able to induce apoptosis.Conclusion: The result proves that ethanolic extract of HCoL and TCa can be developed as a cochemotherapeutic agent with cisplatin to increase the effectivity of breast cancer treatment.

scholarly journals Combination of Tangeretin and 5-Fluorouracil Modulates Cell Cycle and Induce Apoptosis on WiDr Cells

2012 ◽  
Vol 3 (1) ◽  
pp. 364
Author(s):  
Luthfia Indriyani ◽  
Adam Hermawan ◽  
Riris Istighfari Jenie
Keyword(s):  
Cell Cycle ◽  
Colon Cancer ◽  
Cancer Treatment ◽  
Cancer Cells ◽  
Mtt Assay ◽  
Cytotoxic Effect ◽  
Annexin V ◽  
Potential Effect ◽  
Apoptosis Induction ◽  
Cell Cycle Modulation

Co-chemotherapeutics approaches are increasing in cancer treatment in order mainly to suppress the resistence phenomenon of cancer treatment and to enhance the cytotoxic effect of the main chemotherapeutics agent. Tangeretin has been known to have cytotoxic effect to some cancer cells through some pathways in the cells. To explore the potential effect of tangeretin as co-chemotherapeutics agent this research was subjected to study the cytotoxic effect of tangeretin in combination with 5-Fluoro Uracil (5-FU) on WiDr colon cancer cells covering the modulation of cell cycle and apoptosis induction. Cytotoxic effect was examined by using MTT assay while apoptotis induction was determined by annexin-V flowcytometry. Under MTT assay, tangeretin showed weak cytotoxic activity on the cells. However, tangeretin significantly enhanced the cytotoxic effect of 5-FU on the cells. This co-chemotherapeutics effect likely correlated with cell cycle modulation effect, especially in inducing polyploidy phenomenon as expressed in the flowcytometric graph of the DNA content. This combination also increased apoptosis induction. These result suggest that tangeretin is potential to be developed as co-chemotherapeutic agent for 5-Fu on colon cancer and further molecular mechanism need to be explored.Keywords: Tangeretin, 5-Fluorourasil, WiDr, cell cycle, apoptosis.

Sign in / Sign up

Export Citation Format

Share Document