The role of FabH in Listeria monocytogenes fatty acid composition

The sensitivity of Listeria monocytogenes Scott A and ATCC 19114 to antimicrobial compounds was altered when bacterial membrane lipid composition was modified by growth in the presence of added fatty acids. Analysis of cellular fatty acid composition by gas-liquid chromatography indicated that L. monocytogenes Scott A cells contained 0.97, 2.32, 0.81, and 0.72% (relative) of C14:0, C16:0, C18:0, and C18:l, respectively. In the presence of exogenously supplied C14:0, C16:0, C18:0, and C18:l, the percentages increased to 14.03, 30.92, 16.30, and 27.90%. Average MICs for L. monocytogenes Scott A and ATCC 19114 to sodium chloride, tertiary butylhydroquinone, methyl paraben, and propyl paraben were 10.0%, 81, 1406, and 544 μg/ml, respectively. Growing either strain in the presence of 50 μg/ml of either exogenously added C14:0 or C18:0 fatty acids increased their resistance to the four antimicrobial compounds. However, growth in the presence of C18:1 led to increased sensitivity to the antimicrobial agents. The results indicate that the susceptibility of L. monocytogenes to antimicrobial agents is related to the lipid composition of the cell membrane. Consequently, food preservation processes which alter fatty acid composition of L. monocytogenes could result in changes in antimicrobial susceptibility.

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Nisin induces changes in membrane fatty acid composition of Listeria monocytogenes nisin-resistant strains at 10°C and 30°C

Journal of Applied Microbiology ◽

10.1111/j.1365-2672.1997.tb03294.x ◽

1997 ◽

Vol 82 (1) ◽

pp. 32-38 ◽

Cited By ~ 131

Author(s):

A.S. Mazzotta ◽

T.J. Montville

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Listeria Monocytogenes ◽

Acid Composition ◽

Membrane Fatty Acid ◽

Membrane Fatty Acid Composition ◽

Resistant Strains

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Role of lipids in theNeurospora crassa membrane. I. Influence of fatty acid composition on membrane lipid phase transitions

The Journal of Membrane Biology ◽

10.1007/bf01905208 ◽

1977 ◽

Vol 32 (1) ◽

pp. 33-47 ◽

Cited By ~ 15

Author(s):

Kenneth J. Friedman

Keyword(s):

Fatty Acid Composition ◽

Phase Transitions ◽

Fatty Acid ◽

Acid Composition ◽

Membrane Lipid ◽

Lipid Phase ◽

Lipid Phase Transitions

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Regulation Of The Fatty Acid Composition Of Platelet Phospholipids

10.1055/s-0038-1652949 ◽

1981 ◽

Author(s):

M L McKean ◽

J B Smith ◽

M J Silver

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Unsaturated Fatty Acids ◽

De Novo ◽

Membrane Phospholipids ◽

De Novo Biosynthesis ◽

Coa Transferase

The fatty acid composition of cell membrane phospholipids does not remain constant after de novo biosynthesis, but undergoes continual remodelling. One of the major routes for remodelling probably includes the deacylation-reacylation steps of the Lands Pathway. This has been shown to be important for the incorporation of long chain, polyunsaturated fatty acids into phospholipids by liver and brain. An understanding of the mechanisms involved in these processes in platelets is especially important in light of the large stores of arachidonic acid (AA) in platelet phospholipids and the role of AA in hemostasis and thrombosis. Previous results from this laboratory have shown that the turnover of radioactive AA, 8,11,14-eicosatrienoic and 5,8,11,14,17-eicosapentaenoic acids in the phospholipids of resting platelets is more rapid than the turnover of radioactive C16 and C18 saturated and unsaturated fatty acids. However, little is known about how fatty acids, especially AA and its homologues, are incorporated into platelet phospholipids during de novo biosynthesis or how they are exchanged during remodelling.At least three enzymes are involved in the deacylation- reacylation of phospholipids: phospholipase A2; acyl CoA synthetase; and acyl CoA transferase. We have studied acyl CoA transferase and have found considerable activity in human platelet membranes. Experiments are in progress to determine the substrate specificity and other properties of this enzyme.

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