scholarly journals Attenuation of chronic‐binge alcohol‐induced liver injury by Lactobacillus rhamnosus GG culture supernatant via inhibition of intestinal barrier dysfunction (653.12)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Min Zhang ◽  
Yuhua Wang ◽  
Yanlong Liu ◽  
Cuiqing Zhao ◽  
Craig McClain ◽  
...  
Keyword(s):  
Liver Injury ◽  
Culture Supernatant ◽  
Lactobacillus Rhamnosus ◽  
Intestinal Barrier ◽  
Barrier Dysfunction ◽  
Lactobacillus Rhamnosus Gg ◽  
Intestinal Barrier Dysfunction

scholarly journals Lactobacillus rhamnosus GG culture supernatant ameliorates acute alcohol-induced intestinal permeability and liver injury

2012 ◽  
Vol 303 (1) ◽  
pp. G32-G41 ◽  
Author(s):  
Yuhua Wang ◽  
Yanlong Liu ◽  
Anju Sidhu ◽  
Zhenhua Ma ◽  
Craig McClain ◽  
...  
Keyword(s):  
Liver Injury ◽  
Intestinal Permeability ◽  
Culture Supernatant ◽  
Ex Vivo ◽  
Liver Steatosis ◽  
Lactobacillus Rhamnosus ◽  
Intestinal Barrier ◽  
Chow Diet ◽  
Mrna Levels ◽  
Lactobacillus Rhamnosus Gg

Endotoxemia is a contributing cofactor to alcoholic liver disease (ALD), and alcohol-induced increased intestinal permeability is one of the mechanisms of endotoxin absorption. Probiotic bacteria have been shown to promote intestinal epithelial integrity and protect barrier function in inflammatory bowel disease (IBD) and in ALD. Although it is highly possible that some common molecules secreted by probiotics contribute to this action in IBD, the effect of probiotic culture supernatant has not yet been studied in ALD. We examined the effects of Lactobacillus rhamnosus GG culture supernatant (LGG-s) on the acute alcohol-induced intestinal integrity and liver injury in a mouse model. Mice on standard chow diet were supplemented with supernatant from LGG culture (109 colony-forming unit/mouse) for 5 days, and one dose of alcohol at 6 g/kg body wt was administered via gavage. Intestinal permeability was measured by FITC-FD-4 ex vivo. Alcohol-induced liver injury was examined by measuring the activity of alanine aminotransferase (ALT) in plasma, and liver steatosis was evaluated by triglyceride content and Oil Red O staining of the liver sections. LGG-s pretreatment restored alcohol-induced reduction in ileum mRNA levels of claudin-1, intestine trefoil factor (ITF), P-glycoprotein (P-gp), and cathelin-related antimicrobial peptide (CRAMP), which play important roles on intestinal barrier integrity. As a result, LGG-s pretreatment significantly inhibited the alcohol-induced intestinal permeability, endotoxemia and subsequently liver injury. Interestingly, LGG-s pretreatment increased ileum mRNA expression of hypoxia-inducible factor (HIF)-2α, an important transcription factor of ITF, P-gp, and CRAMP. These results suggest that LGG-s ameliorates the acute alcohol-induced liver injury by promoting HIF signaling, leading to the suppression of alcohol-induced increased intestinal permeability and endotoxemia. The use of bacteria-free LGG culture supernatant provides a novel strategy for prevention of acute alcohol-induced liver injury.

scholarly journals Diurnal variations in intestinal barrier integrity and liver pathology in mice: implications for alcohol binge

2018 ◽  
Vol 314 (1) ◽  
pp. G131-G141 ◽  
Author(s):  
Robin M. Voigt ◽  
Christopher B. Forsyth ◽  
Maliha Shaikh ◽  
Lijuan Zhang ◽  
Shohreh Raeisi ◽  
...  
Keyword(s):  
Liver Injury ◽  
Intestinal Permeability ◽  
Intestinal Barrier ◽  
Time Of Day ◽  
Chow Diet ◽  
Barrier Dysfunction ◽  
Barrier Integrity ◽  
Intestinal Barrier Dysfunction ◽  
Daily Variations ◽  
Intestinal Barrier Integrity

Recent studies suggest that circadian rhythms regulate intestinal barrier integrity, but it is not clear whether there are daily variations in barrier integrity. This study investigated daily variations in intestinal barrier integrity, including whether there are differences in alcohol-induced intestinal barrier dysfunction after an alcohol binge at different times of day and whether this is associated with concurrent liver injury. C57BL6/J male mice were fed a standard chow diet, an alcohol-containing liquid diet, or an alcohol control diet for 4 wk. During week 5 (i.e., on days 43–45), mice received three once-daily gavages of alcohol (6 g/kg) or the control (phosphate-buffered saline) at the same time each day. Immediately after the binge on the second day, intestinal permeability was assessed. Four hours after the third and final binge, mice were euthanized and tissue samples collected. The results demonstrated diet-specific and outcome-specific effects of time, alcohol, and/or time by alcohol interaction. Specifically, the alcohol binge robustly influenced markers of intestinal barrier integrity, and liver markers were robustly influenced by time of day. Only intestinal permeability (i.e., sucralose) demonstrated a significant effect of time and also showed a binge by time interaction, suggesting that the time of the alcohol binge influences colonic permeability. NEW & NOTEWORTHY This study investigated daily variations in intestinal barrier integrity, including whether there are differences in alcohol-induced intestinal barrier dysfunction after an alcohol binge at different times of day and whether this is associated with concurrent liver injury. We conclude that 1) alcohol binge significantly impacted markers of intestinal permeability, 2) time of day significantly affected liver outcomes, and 3) the time of day influenced colonic permeability.

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